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1.
Medicentro (Villa Clara) ; 24(2): 373-391, abr.-jun. 2020. graf
Article in Spanish | LILACS | ID: biblio-1124998

ABSTRACT

RESUMEN El Laboratorio de Cirugía Experimental de la Universidad de Ciencia Médicas de Villa Clara cuenta con la tecnología necesaria para el montaje y desarrollo de técnicas y habilidades quirúrgicas y microquirúrgicas, las cuales son utilizadas por los profesionales y estudiantes en la docencia y la investigación biomédica. Se realizaron varias innovaciones con la recuperación de equipos, instrumentos y materiales en desuso; se creó el gimnasio quirúrgico conformado por modelos inanimados sintéticos, y se implementó el modelo «pollo¼, como técnica alternativa al uso de animales vivos en la experimentación animal. Se han desarrollado: importantes proyectos de investigación, entrenamientos de cirugía y microcirugía experimental, diversos programas docentes, y exitosos cursos internacionales con estudiantes y residentes. El laboratorio constituye un valioso aporte de alternativas éticas, económicas y sanitarias, que han favorecido la formación de recursos humanos y un servicio científico-técnico de calidad para la docencia y la investigación.


ABSTRACT The Laboratory of Experimental Surgery at the University of Medical Sciences of Villa Clara has the necessary technology for the assembly and development of skills, as well as, surgical and microsurgical techniques, which are used by professionals and students in teaching and biomedical research. Several innovations were made with the recovery of equipments, instruments and disused materials; the surgical gymnasium was made up of synthetic inanimate models, and the "chicken" model was implemented as an alternative technique to the use of live animals in animal experimentation. Important research projects, training in surgery and experimental microsurgery, diverse teaching programs, and successful international courses with students and residents have been developed. The laboratory constitutes a valuable contribution of ethical, economic and sanitary alternatives, which have favored the formation of human resources and a qualified scientific-technical service for teaching and research.


Subject(s)
Laboratory Equipment , Laboratory Experiment , Animals, Laboratory
2.
Fisioter. Mov. (Online) ; 33: e003339, 2020. graf
Article in English | LILACS | ID: biblio-1133897

ABSTRACT

Abstract Introduction: Many treatment modalities are used for muscle tissue recovery. Photobiomodulation is a modality that can be employed to improve the quality of tissue repair. The use of fractal dimension (FD) is an innovative methodology in the quantitative evaluation of treatment efficacy. Objective: Use FD as a quantitative analysis method to evaluate the effect of photobiomodulation of 904 nanometers (nm) in the initial phase of the muscle regeneration process. Method: Thirty male Wistar rats were divided into three groups: Control Group (CG), Injured and Untreated Group (IUT), and Injured and Treated Group (IT). Muscle injury was induced by cryoinjury in the central region of the anterior tibial (AT) belly of the left posterior limb. This was performed by an iron rod that was previously immersed in liquid nitrogen. Applications started 24 hours after the injury and occurred daily for five days. They were performed at two points in the lesion area. The rats were euthanized on the seventh day. The AT muscles were removed and frozen in liquid nitrogen. Then, the histological sections were stained using the Hematoxylin-Eosin (HE) technique and submitted to FD analysis performed by the box-counting method using ImageJ software. The Kolmogorov-Smirnov test was used for data normality, and the Kruskall-Wallis test and Dunn's post-test were used for group comparison (p<0.05%). Results: Differences between IT and IUT groups were statistically significant, and it was possible to observe the reduction of fractability with p=0.0034. Conclusion: FD is a useful tool for the analysis of skeletal muscle disorganization in the initial phase of regeneration and confirms the potentially beneficial effects of photobiomodulation to this process.


Resumo Introdução: Diversas modalidades de tratamento são utilizadas para recuperação do tecido muscular, dentre elas a fotobiomodulação pode ser empregada para melhorar a qualidade da regeneração e a dimensão fractal se apresenta como uma metodologia inovadora na avaliação quantitativa da eficácia do tratamento. Objetivo: Utilizar a dimensão fractal como método de análise quantitativa do efeito do Laser de Arseneto de Gálio (AsGa) na fase inicial do processo de regeneração muscular. Método: Foram utilizados trinta ratos Wistar, machos divididos em: Grupo Controle (CT), Grupo lesado e não tratado (LNT) e Grupo Lesado e tratado (LT). A lesão muscular foi induzida por criolesão na região central do ventre do músculo tibial anterior (TA) do membro posterior esquerdo, por meio de uma haste de ferro previamente imersa em nitrogênio líquido. As aplicações foram iniciadas 24 horas após a lesão, diariamente, durante cinco dias, em dois pontos na área da lesão. No sétimo dia os animais foram eutanasiados; o músculo TA retirado, congelado em nitrogênio líquido e os cortes histológicos corados com a técnica de Hematoxilina-Eosina para serem então submetidos à análise de dimensão fractal realizada pelo método boxcounting através do software Image J. Para a normalidade dos dados utilizou-se Kolmogorov Smirnov, para as comparações teste de Kruskall-Wallis com pós teste de Dunn (p<0,05%). Resultados: A comparação entre LT e LNT foi estatisticamente significativa, sendo possível observar a redução da fractabilidade com p=0,0034. Conclusão: A dimensão fractal é uma ferramenta útil para análise da desorganização músculo esquelética na fase inicial da regeneração e mostra o potencial efeito benéfico da fotobiomodulação nesse processo.


Subject(s)
Rats , Regeneration , Muscle, Striated , Laser Therapy , Wounds, Penetrating , Fractals , Animals, Laboratory
3.
Rev. bioét. (Impr.) ; 27(3): 542-548, jul.-set. 2019.
Article in Portuguese | LILACS | ID: biblio-1041991

ABSTRACT

Resumo O uso de animais para fins didáticos e de pesquisa requer cuidados específicos. Atualmente, vigora no Brasil a Lei 11.794/2008, que rege parâmetros legais de manejo e conduta neste caso. Esta lei foi acompanhada da instalação ou adequação de comissões de ética em instituições que utilizam animais para ensino e investigação, bem como da criação do Conselho Nacional de Controle de Experimentação Animal. No entanto, apesar dos avanços, especialmente na legislação, ainda não foi consolidada nenhuma grande mudança de comportamento de pesquisadores e alunos de graduação que manuseiam animais em laboratório. A divulgação de informações deixa a desejar, e a prática acaba por repercutir a carência de reflexão ética. Este artigo busca averiguar o atual conhecimento bioético de alunos de graduação e professores com o objetivo de estimular mudanças de conduta. Aprovado CEP-Unioeste CAAE 8563417.8.0000.0107


Abstract Used for education and research, laboratory animals require special care on their handling. Brazilian Law 11,794/2008 establishes the legal parameters for animal manipulation and welfare. It was accompanied by the obligatory installation of the Institutional Ethics Committees on the Use of Animals and the creation of the National Council for Animal Experimentation Control. There have been advances in the field of animal bioethics legislation. However, considering the behavior of those who handle the animals in laboratory environment, especially undergraduate students, these advances are insufficient: the information does not reach them and their attitudes remain in need of ethical reflection. This article seeks to investigate the current bioethical knowledge of undergraduate students and teachers in order to stimulate changes in conduct. Aprovado CEP-Unioeste CAAE: 78563417.8.0000.0107


Resumen El uso de animales con fines didácticos y de investigación requiere cuidados específicos. Actualmente, rige en Brasil la Ley 11.794/2008 que regula los parámetros legales de manejo y conducta en estos casos. Esta ley estuvo acompañada de la instalación o adecuación de comisiones de ética en instituciones que utilizan animales para enseñanza e investigación, así como de la creación del Consejo Nacional de Control de Experimentación Animal. No obstante, a pesar de los avances, especialmente en la legislación, aún no se ha consolidado ninguna gran transformación en el comportamiento de los investigadores y alumnos que manipulan animales en el laboratorio. La divulgación de informaciones es insuficiente, y la práctica acaba reflejando la falta de reflexión ética. Este artículo procura identificar el conocimiento bioético actual de los alumnos de grado y de los profesores, con el objetivo de estimular cambios en la conducta. Aprovado CEP-Unioeste CAAE: 78563417.8.0000.0107


Subject(s)
Bioethics , Animal Experimentation , Ethics , Animal Use Alternatives , Animal Care Committees , Animals, Laboratory
4.
Article in English | WPRIM | ID: wpr-739531

ABSTRACT

Scratching is a main behavioral response accompanied by acute and chronic itch conditions, and has been quantified as an objective correlate to assess itch in studies using laboratory animals. Scratching has been counted mostly by human annotators, which is a time-consuming and laborious process. It has been attempted to develop automated scoring methods using various strategies, but they often require specialized equipment, costly software, or implantation of device which may disturb animal behaviors. To complement limitations of those methods, we have adapted machine learning-based strategy to develop a novel automated and real-time method detecting mouse scratching from experimental movies captured using monochrome cameras such as a webcam. Scratching is identified by characteristic changes in pixels, body position, and body size by frame as well as the size of body. To build a training model, a novel two-step J48 decision tree-inducing algorithm along with a C4.5 post-pruning algorithm was applied to three 30-min video recordings in which a mouse exhibits scratching following an intradermal injection of a pruritogen, and the resultant frames were then used for the next round of training. The trained method exhibited, on average, a sensitivity and specificity of 95.19% and 92.96%, respectively, in a performance test with five new recordings. This result suggests that it can be used as a non-invasive, automated and objective tool to measure mouse scratching from video recordings captured in general experimental settings, permitting rapid and accurate analysis of scratching for preclinical studies and high throughput drug screening.


Subject(s)
Animals , Animals, Laboratory , Behavior, Animal , Body Size , Complement System Proteins , Decision Trees , Drug Evaluation, Preclinical , Humans , Injections, Intradermal , Machine Learning , Methods , Mice , Pruritus , Research Design , Sensitivity and Specificity , Video Recording
5.
Article in English | WPRIM | ID: wpr-760473

ABSTRACT

No abstract available.


Subject(s)
Animals, Laboratory
6.
Biomedical Engineering Letters ; (4): 311-325, 2019.
Article in English | WPRIM | ID: wpr-785521

ABSTRACT

Preclinical neuroimaging allows for the assessment of brain anatomy, connectivity, and function in laboratory animals, such as mice and this imaging field has been a rapidly growing aimed at bridging the translation gap between animal and human research. The progress in the animal research could be accelerated by high-resolution in vivo optical imaging technologies. Optical coherence tomography-based angiography (OCTA) estimates the scattering from moving red blood cells, providing the visualization of functional micro-vessel networks within tissue beds in vivo without a need for exogenous contrast agents. Recent advancement of OCTA methods have expanded its application to neuroimaging of small animal models of brain disorders. In this paper, we overview the recent development of OCTA techniques for blood flow imaging and its preclinical applications in neuroimaging. In specific, a summary of preclinical OCTA studies for traumatic brain injury, cerebral stroke, and aging brain on mice is reviewed.


Subject(s)
Aging , Angiography , Animal Experimentation , Animals , Animals, Laboratory , Brain , Brain Diseases , Brain Injuries , Contrast Media , Erythrocytes , Humans , Mice , Models, Animal , Neuroimaging , Optical Imaging , Stroke , Tomography, Optical Coherence
7.
São Paulo; s.n; 2018. 74 p.
Thesis in Portuguese | LILACS | ID: biblio-909912

ABSTRACT

Doenças como a dengue, chikungunya, Zika e febre amarela urbana são causadas por patógenos transmitidos pela picada de fêmeas infectadas de Ae. aegypti e Ae. albopictus. Fatores bióticos, como a competição na fase larval, e abióticos, como a variação da temperatura, provocam alterações no ciclo de desenvolvimento dessas espécies. O presente estudo avaliou os efeitos da competição intraespecífica e da temperatura na atividade locomotora de fêmeas virgens de Ae. aegypti e Ae. albopictus. Os objetivos do trabalho foram: comparar o tamanho das fêmeas de Ae. aegypti e Ae. albopictus oriundas de criadouros com baixa e alta competição intraespecífica; avaliar o efeito da competição intraespecífica na atividade locomotora de fêmeas virgens de Ae. aegypti e Ae. albopictus, sob regime de 12 horas de claro e 12 horas de escuro, à 25ºC e analisar o padrão da atividade locomotora de fêmeas virgens de Ae. aegypti e Ae. albopictus, sob regime de 12 horas de claro e 12 horas de escuro, sob diferentes temperaturas (20ºC, 25ºC e 30ºC). As colônias dos mosquitos utilizados nos experimentos são provenientes da FIOCRUZ-RJ. Para o experimento de competição, larvas de ambas as espécies foram criadas sob condições de alta (100 larvas) e baixa (20 larvas) densidades. Para isso, foram utilizadas 14 bacias preenchidas com 500 ml de água e 0,2 g de ração para peixes. Após a competição, as fêmeas oriundas de cada densidade foram transferidas para incubadoras para a realização da análise da atividade locomotora. Após a atividade, verificou-se o tamanho dos adultos oriundos de cada densidade larvária, por meio da medição das asas de cada fêmea. Para a análise da atividade locomotora sob diferentes temperaturas, as fêmeas utilizadas foram desenvolvidas em ambiente com quantidade de ovos similares. Foi utilizado o mesmo procedimento para análise da atividade do experimento anterior, porém, cada experimento ocorreu sob diferentes temperaturas: 20°C, 25°C e 30°C. Para a análise dos dados obtidos foram utilizados os testes T e ANOVA multifatorial. Para o experimento de competição, fêmeas de ambas as espécies oriundas de competição de baixa densidade foram maiores do que as oriundas de desenvolvimento sob alta densidade larvária. A atividade das fêmeas de Ae. aegypti e Ae. albopictus de ambas as densidades foi muito semelhante, sugerindo que fêmeas menores tem atividade equivalente à de fêmeas maiores. Já no experimento que avaliou a atividade das fêmeas sob faixas térmicas distintas, notou-se que, em Ae. aegypti, houve diferença significativa na atividade total sob 20°C quando comparadas a 25°C e 30°C, demonstrando que a temperatura mais baixa foi limitante para a atividade desta espécie. Houve diferenças em algumas médias avaliadas na atividade de Ae. albopictus, porém, esta espécie pareceu ter sido menos influenciada com a variação da temperatura. Análises estatísticas confirmaram que as fêmeas de Ae. aegypti respondem de forma distinta das fêmeas de Ae. albopictus quando as temperaturas foram modificadas. O presente estudo ajuda a esclarecer alguns aspectos relacionados ao comportamento dos mosquitos Ae. aegypti e Ae. albopictus, que são considerados de grande importância para a saúde pública


Diseases such as dengue, chikungunya, Zika and urban yellow fever are caused by pathogens transmitted by the bites of infected females of Ae. aegypti and Ae. albopictus. Biotics factors essentially competition on larval stages, and abiotics factors, for instance, temperature variation, occasion adjustments on development cycle of these species. The present study evaluated the effects of intraspecific competition and temperature on locomotor activity of Ae. aegypti and Ae. albopictus. The aims of this study were: to compare the size of females Ae. aegypti and Ae. albopictus from breeding sites with low and high intraspecific competition; to evaluate the effect of intraspecific competition on locomotor activity of virgin females of Ae. aegypti and Ae. albopictus, under regime of 12 hours of light and 12 hours of dark at 25°C and to analyze the pattern of the locomotor activity of virgins females of Ae. aegypti and Ae. albopictus, under regime of 12 hours of light and 12 hours of dark, under different temperatures (20ºC, 25ºC e 30ºC). The mosquitoes colonies used on experiment are from FIOCRUZ-RJ. For the competition experiment, larvae from both species were raised under conditions of high (100 larvae) and low (20 larvae) densities. For this 14 containers filled with 500 ml of water and 0,2 g of fish food were used. After the competition, the females from each density were transferred to incubators to perform locomotor activity analysis. After the activity, the size of adult from each larval density, were verified by wings measurement of each female. For the analysis of the locomotor activity under different temperatures, the females used were developed in an environment with similar amount of eggs. The same procedure was used to the analyze the activity of the previous experiment, however, each experiment happened under different temperatures: 20°C, 25°C e 30°C. For the analysis of the obtained data were applied T tests and ANOVA multivariate. For the competition experiment, females of both species from low density of competition were longer than females from competition under high larval density. The activity of females of Ae. aegypti and Ae. albopictus from both densities was very similar, suggesting that smaller females have equivalent activity to that of larger females. In the experiment that evaluated the activity of females under different thermal bands, it was noticed that in Ae. aegypti there was significant difference in total activity under 20°C when compared with 25°C and 30°C degrees, demonstrating the lower temperature was limiting to activity for this species. There were differences in some averages evaluated in Ae. albopictus activity, however, this species seemed to have been less influenced with the temperature variation. Statistical analysis confirmed that females of Ae. aegypti respond differently than females of Ae. albopictus when the temperatures were modified. The present study helps to clarify some aspects about the behavior of mosquitoes Ae. aegypti and Ae. albopictus which are considered of great concern for public health


Subject(s)
Animals , Aedes/growth & development , Competitive Behavior , Insect Vectors , Locomotion , Temperature , Analysis of Variance , Animals, Laboratory , Larva , Species Specificity
8.
Rev. Inst. Nac. Hig ; 49(2): 52-63, 2018. ilus, graf
Article in Spanish | LILACS, LIVECS | ID: biblio-1096359

ABSTRACT

Se plantea como objetivo describir las técnicas de necropsia en animales de experimentación, una revisión bibliográfica y actualización. Se describieron las técnicas de necropsia en cobayos (Cavia porcellus), conejos (Oryctolaguscuniculus), ratones (Mus musculus), la rata (Rattusnorvegicus (variedad albina), incluyendo peces. La necropsia sistemática incluye una revisión de la historia clínica, y/o datos del experimento realizado. El siguiente paso corresponde al examen externo, donde se describen lesiones sobre el tejido tegumentario, sistema locomotor, mucosas, cavidades naturales y cambios post-mortem. Posteriormente se realiza el desollado, de manera simultánea la apertura de cavidades, con la evaluación de órganos, mediante un exhaustivo examen macroscópico. Este procedimiento continua con la extracción de órganos y toma de muestras. Por último se debe elaborar un informe de necropsia donde el patólogo veterinario emitirá las conclusiones o diagnósticos comentarios, recomendaciones y las apreciaciones enmarcados en la epicrisis del caso. El proceso de necropsia junto con la recolección y envío de muestras apropiadas para la realización de pruebas de laboratorio es trascendental en el proceso de emisión de un diagnóstico, de la práctica de la toma de muestra y envío al laboratorio depende el diagnostico morfológico y etiológico. En conclusión se describieron las técnicas de necropsia y toma de muestras en animales de experimentación con énfasis en cobayos (Cavia porcellus), conejos (Oryctolaguscuniculus), ratones (Mus musculus), la rata (Rattusnorvegicus, variedad albina), incluyendo peces, como una herramienta básica para el diagnostico de patologías en animales de experimentación así como se mencionaron las condiciones de envío de muestras a los distintos laborator


The aim of this study was to describe the necropsy techniques in experimental animals, a literature review and update. Necropsy techniques were described in guinea pigs (Cavia porcellus), rabbits (Oryctolagus cuniculus), mice (Mus musculus), rat (Rattus norvegicus (albino variety), including fish, systematic necropsy includes a review of clinical history, and/or data of the experiment performed. The next step corresponds to the external examination, where lesions are described on the integumentary tissue, locomotor system, mucous membranes, natural cavities and post-mortem changes, followed by flaying, simultaneously opening cavities, with the evaluation of organs, through an exhaustive macroscopic examination. This procedure continues with the extraction of organs and sampling. Finally, a necropsy report must be prepared where the veterinary pathologist will issue the conclusions or diagnoses comments, recommendations and assessments framed in the epicrisis of the case, the necropsy process together with the collection and shipment of appropriate samples for the performance of laboratory tests is crucial in the process of issuing a diagnosis, the practice of sampling and sending to the laboratory depends on the morphological and etiological diagnosis. In conclusion, necropsy and sampling techniques were described in experimental animals with emphasis on guinea pigs (Cavia porcellus), rabbits (Oryctolagus cuniculus), mice (Mus musculus), rat (Rattus norvegicus albino variety), including fish, as a basic tool for the diagnosis of pathologies in experimental animals, as well as the conditions for sending samples to different laboratories.


Subject(s)
Animals , Male , Female , Guinea Pigs , Mice , Rabbits , Rats , Pathology , Specimen Handling , Animal Experimentation , Animals, Laboratory
9.
10.
Rev. Inst. Nac. Hig ; 49(1): 57-75, 2018. ilus, graf
Article in Spanish | LILACS, LIVECS | ID: biblio-1096215

ABSTRACT

La Gerencia de Producción y Servicios Básicos del INHRR responde a las necesidades de las áreas técnicas del Instituto a través de la producción de insumos requeridos para el control de productos de uso y consumo humano, diagnóstico de enfermedades transmisibles, desarrollo de nuevas técnicas y líneas de investigación, orientadas a garantizar la salud integral de la población venezolana; tales como: animales de laboratorio, hemoderivados de uso diagnóstico, cultivos celulares, medios de cultivo, reactivos, colorantes, agua purificada, agua calidad inyectable y estuches biológicos. Además brinda servicios auxiliares de descontaminación, lavado, embalaje, esterilización de materiales y uniformes de laboratorio. Presta servicio de secuenciación de ácidos nucleicos a los laboratorios del Instituto y usuarios externos, así como también asesoría técnica y científica a entes internos, nacionales e internacionales en actividades de su competencia


The Production and Basic Services Management of INHRR responds to the needs of the technical areas of the Institute through the production of inputs required for the control of products of human use and consumption, diagnosis of transmissible diseases, development of new techniques and lines of research aimed at guaranteeing the integral health of the Venezuelan population; such as: laboratory animals, blood products for diagnostic use, cell cultures, culture media, reagents, dyes, purified water, water for injection and biological kits. It also provides auxiliary services of decontamination, washing, packaging and sterilization of materials and laboratory uniforms. Also it provides nucleic acid sequencing service to the Institute´s laboratories and external users, as well as technical and scientific adviser to internal, national and international entities in activities of their competence


Subject(s)
Humans , Male , Female , Reagent Kits, Diagnostic , Biological Products , Culture Media , Health Facilities , Animals, Laboratory , Public Health , History of Medicine
11.
JCPSP-Journal of the College of Physicians and Surgeons Pakistan. 2018; 28 (3): 192-195
in English | IMEMR | ID: emr-163436

ABSTRACT

Objective: To observe the effects of Shisha smoke on submucosal glands of trachea of mice; and compare it with tracheal glands of mice exposed to cigarette smoke


Study Design: Randomised controlled trial


Place and Duration of Study: Department of Anatomy, Islamic International Medical College, Rawalpindi, in collaboration with National Institute of Health [NIH], Islamabad from October 2013 till April 2014


Methodology: Sample comprised of 40 adult male mice of strain BALB/c. They were randomly divided into three groups. Control group was labelled as Group 'C'. The mice in this group were kept in a whole body smoke exposure chamber and were exposed to fresh air. Shisha group was labelled as Group 'SS', and the mice in this group were exposed to Shisha smoke. Mice in the third group labelled as Group CS were exposed to cigarette smoke. All the mice were dissected after an exposure period of eight weeks. Tracheal tissue was stained and examined microscopically for submucosal gland hypertrophy and compared with the control group, using Reid's Index. An Index of more than 0.4 is termed as hypertrophy


Results: There was significant submucosal gland hypertrophy in groups CS and SS as compared to group C. There was also significant difference in the frequency of mucosal hypertrophy between SS [93.7%] and CS groups [53.3%], which was found statistically significant [p<0.001]


Conclusion: Shisha smoking was significantly associated with mucosal hypertrophy when compared with cigarette smoking and controls. Shisha smoke contains higher level of toxicants as compared to cigarette smoke, and it causes more oxidative damage of tissues


Subject(s)
Animals, Laboratory , Smoking Water Pipes , Trachea/pathology , Trachea/anatomy & histology , Mice
12.
IBJ-Iranian Biomedical Journal. 2018; 22 (2): 90-98
in English | IMEMR | ID: emr-192455

ABSTRACT

Background: Hypothyroidism is associated with dysfunction of the bone turnover with reduced osteoblastic bone formation and osteoclastic bone resorption. Mesenchymal stem cells [MSCs] secrete various factors and cytokines that may stimulate bone regeneration. The aim of this study was to determine the effects of MSCs-conditioned medium [CM] in hypothyroidism male rats after inducing bone defect


Methods: In this study, 24 male rats were randomly assigned to three groups: [I] hypothyroidism + bone defect [HYPO], [II] hypothyroidism + bone defect + CM [HYPO + CM], and [III] no hypothyroidism + bone defect [control]. Four weeks after surgery, the right tibia was removed, and immediately, biomechanical and histological examinations were performed


Results: The results showed a significant reduction in bending stiffness [32.64 +/- 3.99], maximum force [14.63 +/- 1.89], high stress load [7.59 +/- 2.31], and energy absorption [12.68 +/- 2.12] at the osteotomy site in hypothyroidism rats in comparison to the control and hypothyroidism + condition medium groups [p < 0.05]. There was also a significant decrease in the trabecular bone volume [3.86 +/- 3.88] and the number of osteocytes [5800 +/- 859.8] at the osteotomy site in hypothyroidism rats compared to the control and hypothyroidism + condition medium groups [p < 0.01 and p < 0.02, respectively]


Conclusion: The present study suggests that the use of the CM can improve the fracture regeneration and accelerates bone healing at the osteotomy site in hypothyroidism rats


Subject(s)
Animals, Laboratory , Culture Media, Conditioned , Hypothyroidism/veterinary , Osteotomy , Tibial Fractures , Fracture Healing
13.
IBJ-Iranian Biomedical Journal. 2018; 22 (3): 151-159
in English | IMEMR | ID: emr-192464

ABSTRACT

Background: The majority of male patients with spinal cord injury [SCI] suffer from infertility. Nucleotide-binding oligomerization domain-like receptors NOD-like receptors [NLRs] are a kind of receptors that corporate in the inflammasome complex. Recent studies have introduced the inflammasome as the responsible agent for secreting cytokines in semen. Reactive oxygen species [ROS] is one of the elements that trigger inflammasome activation. Genital infections in SCI can lead to ROS generation. We investigated the relation between lipid peroxidation and inflammasome complex activity in testicular tissue of SCI rats


Methods: Adult male rats [n=20], weighting 200- 250 g, were included and divided into four groups: three experimental groups, including SCI1, SCI3, and SCI7, i.e. the rats were subjected to SCI procedure and sacrificed after one, three, and seven days, respectively and a control group. We performed a moderate, midline spinal contusion injury at thoracic level 10. The animals were anesthetized, and testes were collected for measurement of gene expression by real-time PCR. Caudal parts of epididymis were collected for malondialdehyde [MDA] measurement


Results: No NLRP1a mRNA overexpression was seen in the testes of control and SCI groups. After seven days from SCI surgery, NLRP3 mRNA expression was significantly increased in SCI7 animals [p

Conclusion: NLRP3 overexpression occurs due to the increased ROS production in testis tissue of SCI rats


Subject(s)
Animals, Laboratory , Infertility , Lipid Peroxidation/genetics , NLR Family, Pyrin Domain-Containing 3 Protein , Reactive Oxygen Species , Real-Time Polymerase Chain Reaction , Gene Expression , Testis , Rats, Wistar
14.
IBJ-Iranian Biomedical Journal. 2018; 22 (3): 171-179
in English | IMEMR | ID: emr-192466

ABSTRACT

Background: Curcumin, extracted from turmeric, represents enormous potential to serve as an anticancer agent. Telomerase is viewed as a prominent molecular target of curcumin, and transforming growth factor- beta 1 [TGF beta 1] has proven to be a major inhibitory signaling pathway for telomerase activity. In the current study, we aimed to explore suppressive effects of nanocurcumin on telomerase expression through TGF beta 1 pathway in a hepatocellular carcinoma cell line [Huh7]


Methods: MTT assay was used to determine the effect of nonocurcumin on viability of Huh7 cells. RT-PCR was used to analyze the gene expression patterns


Results: MTT assay revealed that nanocurcumin acts in a dose- and time-dependent manner to diminish the cell viability. RT-PCR analysis indicated that nanocurcumin results in augmentation of TGF beta 1 72 hours post treatment and leads to the reduction of telomerase expression 48 and 72 hours post exposure. Also, up-regulation of Smad3 and E2F1 and down-regulation of Smad7 confirmed the effect of nanocurcumin on intermediate components of TGF beta 1 pathway. Furthermore, transfection of the proximal promoter of telomerase triggered a significant reduction in luciferase activity


Conclusion: The data from the present study lead us to develop a deeper understanding of the mechanisms underlying nanocurcumin-mediated regulation of telomerase expression, thereby presenting a new perspective to the landscape of using nanocurcumin as a cancer-oriented therapeutic agent


Subject(s)
Animals, Laboratory , Liver Neoplasms/therapy , Liver Neoplasms/genetics , Curcumin/therapeutic use , Telomerase , Gene Expression , Transforming Growth Factor beta1
15.
Egyptian Journal of Hospital Medicine [The]. 2018; 71 (4): 2885-2891
in English | IMEMR | ID: emr-192543

ABSTRACT

Background: Insulin resistance is a pathological condition characterized by inadequate peripheral tissue metabolic response to circulating insulin. High dietary fructose causes insulin resistance syndrome, primarily due to simultaneous induction of genes involved in glucose, lipid and mitochondrial oxidative metabolism. Rice bran oil [RBO] is a rich source of antioxidants which contribute to higher oxidative stability and longer shelf life than other edible oils


Aim of the work: The current study investigated the effects of the daily intake of RBO on insulin resistant rat liver, as a central organ in carbohydrate metabolism


Materials and methods: Rats were allocated in 5 groups. Animals in groups 1 and 2 received standard diet and standard diet containing RBO, respectively. Group 3: animals fed high fructose diet [HFD], which was categorized into: rats fed HFD either for one month [HFD1] or for 2 months [HFD2]. Group 4, rats were fed HFD containing RBO for one month [HFD1+RBO], while rats in group 5 were fed HFD for 30 days then RBO was added to the diet for another 30 days [HFD2+RBO]


Results and conclusion: addition of RBO to this model improved insulin sensitivity in liver


Subject(s)
Animals, Laboratory , Insulin Resistance , Fructose , Liver , Carbohydrate Metabolism , Rats , Glycogen
16.
Egyptian Journal of Hospital Medicine [The]. 2018; 71 (4): 3049-3055
in English | IMEMR | ID: emr-192565

ABSTRACT

Background: alterations in the level of hormones including thyroid hormones lead to physiological/ clinical abnormalities


Aim of work: this study aimed to illustrate the protective effect of Trigonella, Allium sativum or their mixture against hyperthyroidism induced by L-thyroxin in male albino rats


Materials and methods: thirty albino rats were divided randomly into five groups. Group A: healthy rats, Group B: normal rats were subcutaneously injected with 500 micro g/kg body weight L-thyroxin once daily for two weeks to induce hyperthyroidism, Group C: hyperthyroid rats received oral dose of 250 micro g/kg body weight/day Trigonella extract. Group D: hyperthyroid rats received oral dose of 250 micro g/kg body weight/day Allium sativum extract. Group E: hyperthyroid rats received mixture of Trigonella and Allium sativum extract. At the end of the experiment. Blood samples were collected for biochemical analysis


Results: Hyperthyroid rats had significant increase [P<0.05] in serum levels of triodothyronine [T[3]], thyroxin [T[4]], TG, VLDL,AST,ALP,BUN and uric acid as well as a significant decrease in TSH, LDL, HDL, LDL/HDL and TC, total protein and percentage of body weight change compared to the normal control group. Oral administration of these extracts ameliorated most of the tested parameters


Conclusion: This study concluded that Allium sativum,Trigonella or their mixture have a protective effect against hyperthyroidism


Subject(s)
Animals, Laboratory , Trigonella , Garlic , Rats , Protective Agents , Complex Mixtures , Plant Extracts , Thyroxine
18.
Arab Journal of Gastroenterology. 2017; 18 (2): 87-97
in English | IMEMR | ID: emr-189171

ABSTRACT

Background and study aims: The therapeutic effects of human umbilical cord-derived mesenchymal stem cells [UC-MSCs] exposed to diode laser and/or hepatocyte growth factor [HGF] were compared in mice with experimental liver fibrosis induced by carbon tetra chloride [CCl[4]]


Material and methods: Animal model of liver cirrhosis was induced by intraperitoneal injection of CCl[4] in a dose of 0.4 ml/kg, twice a week for 6 weeks. UC-MSCs were obtained from normal full term placentas and were exposed to diode laser and/or HGF. Before treatment, UC-MSCs were labelled with red fluorescent PKH26. Fifty four male mice weighing 25-35 g were randomly divided into four groups control, stem cells, CCl[4], and treated groups. After the experimental period, body and liver weights were recorded, and the liver specimens were processed for histological examination using haematoxylin and eosin, Periodic Acid-Schiff [PAS], and Masson's Trichrome staining [MT]


Results: Results showed that administration of UC-MSCs stimulated by diode laser and/or HGF improved body and liver weights, reduced vascular dilatation and congestion, reduced mononuclear cellular infiltration, reduced hepatocyte vacuolation, eosinophilia, and pyknosis. Furthermore, periportal fibrosis was minimized and PAS reaction was increased. These effects were maximum when UC-MSCs were exposed to both diode laser and HGF


Conclusion: UC-MSCs stimulated by both diode laser and HGF proved to be an effective therapeutic option in experimental liver fibrosis induced by CCl[4] in mice


Subject(s)
Animals, Laboratory , Lasers , Intercellular Signaling Peptides and Proteins , Stem Cells , Mesenchymal Stem Cells , Mice , Lasers, Semiconductor , Hepatocyte Growth Factor , Carbon Tetrachloride
19.
Egyptian Journal of Hospital Medicine [The]. 2017; 67 (1): 322-329
in English | IMEMR | ID: emr-189183

ABSTRACT

Introduction: The present study was carried out to evaluate the effect of the beta-lactam antibiotic amoxicillin on the fetuses of albino mice from the morphological and skeletal points of view


Material and methods: Twenty four adult pregnant mice were used in the present study. They were allocated into 3 groups [8 mice each]. The first group served as a control and were injected intraperitoneally [i.p.] with the solvent of the drug and the second and third groups were treated with 205 and 820 mg/kg body weight of amoxicillin for 8 days [gestation days 7-14], respectively


Results: The morphological examination of the fetuses of treated groups showed growth retardation of mice fetuses as represented by the conspicuous decrease in the average body weight and body length in the two treated groups. No external malformations were recorded among fetuses maternally treated with the low dose of the drug. On the other hand, the fetuses maternally treated with the high dose showed mild external morphological malformations. In addition, the skeleton of the two treated groups exhibited incomplete ossification in most skeletal elements


Conclusion: The beta-lactam antibiotic amoxicillin had exerted mild morphological malformations and skeletal abnormalities in mice fetuses maternally treated during organogenesis period of gestation


Subject(s)
Animals, Laboratory , Fetus/drug effects , Monobactams , Mice , Skeleton/drug effects , Pregnancy, Animal
20.
Egyptian Journal of Hospital Medicine [The]. 2017; 67 (1): 330-335
in English | IMEMR | ID: emr-189184

ABSTRACT

Background: food additives are added to most junk and fast foods, especially those for kids. Sodium nitrite is an inorganic salt with widespread applications in the food industry as a color fixative and preservative in meat and fish. Annatto extract is a natural food color obtained from the outer coatings of the seeds of the Annatto tree [Bixa orellana L.]. Monosodium glutamate [MSG], the sodium salt of amino acid glutamate, is a food additive that popularly used all over the world as "flavor enhancer"


Aim of the work: this study was aimed to determine the hazardous effects of sodium nitrite, annatto and monosodium glutamate on some physiological parameters in male albino rats


Materials and methods: this study had been done on fourty male albino rats with an average body weight 100-145 g. The animals were divided into four groups; Group 1: control [untreated group], Group 2: sodium nitrite treated group, Group 3: annatto treated group and Group 4: monosodium glutamate treated group. Blood samples were collected, sera were separated and used for estimation of some biochemical parameters [liver enzymes, kidney functions, glucose, protein profile and lipid profile] and hormonal levels [testosterone, T3 [triiodothyronine] and T4 [thyroxine]]


Results: the biochemical results showed an increase in the activities of liver enzymes [aspartate aminotransferase [ASAT] and alanine aminotransferase [ALAT]], and the levels of glucose, kidney functions [urea, and creatinine], lipid profile [total cholesterol, triglycerides, low density lipoprotein [LDL-C]] and thyroid hormones [thyroxin [T4] and triiodothyronine [T3]] in all treated groups when compared to the control group. A drop in protein profile [total protein, albumin, globulin and A/G ratio], testosterone hormone and HDL level were observed in the treated groups as compared to the control rats


Conclusion: it could be concluded that some food additives like sodium nitrite, annatto, and monosodium glutamate have extreme effects on liver and kidney functions, protein and lipid profiles and also on thyroid and testosterone hormones. So, it is recommended to minimize the use of these additives to protect young children and mature people from these destructive effects


Subject(s)
Animals, Laboratory , Carotenoids , Plant Extracts , Bixaceae , Sodium Glutamate/pharmacology , Rats , Liver Function Tests , Liver/drug effects , Kidney Function Tests , Kidney/drug effects
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