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1.
Rev. chil. enferm. respir ; 38(1): 20-25, mar. 2022. ilus, tab
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1388168

ABSTRACT

INTRODUCCIÓN: Las Vasculitis Asociadas a Anticuerpos Anticitoplasma de Neutrófilos (VAA) son infrecuentes, pero de amplio espectro de presentación. Si bien el consenso de clasificación de Chapel Hill del año 2012, sigue vigente, la tendencia actual es clasificarlas de acuerdo al marcador inmunológico: anti-Proteinasa 3 (PR3) o anti-mieloperoxidasa (MPO). Las manifestaciones pulmonares clásicas son la hemorragia alveolar y los nódulos pulmonares. En los últimos 10 años se ha descrito la enfermedad pulmonar difusa (EPD). Los estudios epidemiológicos son escasos, y suelen representar en su mayoría poblaciones norteamericanas o europeas. El objetivo es describir las características del compromiso pulmonar al debut en VAA en un centro universitario. PACIENTES Y MÉTODO: De un total de 190 pacientes con diagnóstico de VAA se incluyeron 170 en seguimiento en nuestro centro. Se revisaron aspectos clínicos, demográficos, laboratorio e imagenológicos de los pacientes incluidos. RESULTADOS: De los 170 pacientes, 112 (65,88%) presentaron compromiso pulmonar. 106 (94,64%) de los pacientes fueron anticuerpos anti citoplasma de neutrófilos (ANCA) positivos; de estos, 56 (53,27%) MPO (+) y 39 (36,45%) PR-3 (+). Un tercio de los pacientes de ambos grupos presentó hemorragia alveolar. En los pacientes MPO (+) predomina la EPD (53,5%) y en PR-3 (+) los nódulos pulmonares (69,23%). Destaca la baja frecuencia de patología obstructiva asociada. CONCLUSIONES: El compromiso pulmonar en las VAA es prevalente y heterogéneo. En nuestra serie, destaca la frecuencia de EPD en VAA MPO (+), lo que releva la importancia del estudio con ANCA en paciente con diagnóstico y seguimiento por EPD.


INTRODUCTION: Antineutrophil Cytoplasmic Antibodies (ANCA) associated vasculitis (AAV) are uncommon, but of broad spectrum of presentation. Although the 2012 Chapel Hill classification consensus remains valid, the current trend is to classify them according to the immunological marker: anti-Proteinase 3 antibody (PR-3) or anti-Myeloperoxidase antibody (MPO). The classic pulmonary manifestations are alveolar hemorrhage and pulmonary nodules. Interstitial lung disease (ILD) has been described in the last 10 years. Epidemiological studies are scarce, and they usually represent mostly North American or European populations. The objective is to describe the characteristics of lung involvement upon debut in AAV in a university center. PATIENTS AND METHODS: Of a total of 190 patients diagnosed with AAV, 170 were included in follow-up at our center. Clinical, demographic, laboratory and imaging aspects of the included patients were reviewed. RESULTS: Of the 170 patients, 112 (65.88%) had lung involvement. 106 (94.64%) of the patients were ANCA (+); of these, 56 (53.27%) MPO (+) and 39 (36.45%) PR-3 (+). One third of the patients in both groups had alveolar hemorrhage. In MPO (+) patients, ILD predominates (53.5%) and in PR-3 (+) pulmonary nodules (69.23%). The low frequency of associated obstructive pathology stands out. CONCLUSIONS: Pulmonary manifestations in AAVs are frequent and heterogeneous. Locally, the association of ILD and AAV MPO (+) stands out, which highlights the importance of ANCA study in patients with diagnosis and follow-up by ILD.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Lung Diseases, Interstitial/diagnosis , Lung Diseases, Interstitial/etiology , Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/complications , Biomarkers/analysis , Retrospective Studies , Follow-Up Studies , Lung Diseases, Interstitial/epidemiology , Lung Diseases, Interstitial/diagnostic imaging , Antibodies, Antineutrophil Cytoplasmic , Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/classification , Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/epidemiology , Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/diagnostic imaging , Inflammation/etiology , Antibodies/analysis
2.
Arq. Asma, Alerg. Imunol ; 6(1): 127-133, jan.mar.2022. ilus
Article in English, Portuguese | LILACS | ID: biblio-1400122

ABSTRACT

A doença de Castleman é um distúrbio linfoproliferativo raro, podendo se manifestar sob a forma de massas localizadas ou como doença multicêntrica. A doença de Castleman multicêntrica é caracterizada por adenopatias generalizadas, visceromegalias, manifestações autoimunes e infecções recorrentes. Este artigo apresenta o relato de caso de anemia hemolítica autoimune por anticorpos quentes em paciente com doença de Castleman multicêntrica. Resposta eficaz foi obtida com uso de corticoterapia sistêmica e tocilizumabe.


Castleman disease is a rare lymphoproliferative disorder that can manifest as localized masses or as multicentric disease. Multicentric Castleman disease is characterized by generalized adenopathies, visceromegaly, autoimmune manifestations, and recurrent infections. This article presents the case report of a patient with multicentric Castleman's disease and autoimmune hemolytic anemia by warm antibodies. Effective response was obtained with systemic corticotherapy and tocilizumab.


Subject(s)
Humans , Male , Adult , Castleman Disease , Anemia, Hemolytic, Autoimmune , Patients , Adrenal Cortex Hormones/therapeutic use , Antibodies, Monoclonal, Humanized , Lymphoproliferative Disorders , Antibodies
3.
Medicina UPB ; 41(1): 85-90, mar. 2022. Ilus
Article in Spanish | LILACS, COLNAL | ID: biblio-1362717

ABSTRACT

Se presenta el caso de una mujer en la quinta década de la vida que ingresa al servicio de urgencias con manifestaciones gastrointestinales consistentes en vómito e hipo. Después de múltiples estudios e intervenciones por especialistas, se piensa en el origen central de los síntomas. Se realiza una resonancia magnética nuclear (RMN) cerebral que permite el enfoque del caso y posterior confirmación del diagnóstico de una enfermedad dentro del espectro de la neuromielitis óptica, positiva para anticuerpos anti-acuaporinas-4. El tratamiento con esteroide y anticuerpo monoclonal (Rituximab) llevan a un control adecuado de la enfermedad.


We present a case report of a woman in her 50s admitted to the emergency room with gastrointestinal manifestations consisting of vomiting and hiccups. After a series of studies and interventions by specialists, a brain magnetic resonance imaging (MRI) is performed in order to find the central origin of the symptoms. This allows the approach of the case and subsequent confirmation of the diagnosis of Neuromyelitis optica, positive for anti-acuaporin-4 antibodies. Finally, it seems that treatment with steroids and monoclonal antibodies leads to proper control of the disease.


Se apresenta o caso de uma mulher na quinta década de vida que ingressa ao serviço de urgências com manifestações gastrointestinais consistentes em vômito e soluço. Depois de múltiplos estudos e intervenções por especialistas, se pensa na origemcentral dos sintomas. Se realiza uma ressonância magnética nuclear (RMN) cerebral que permite o enfoque do caso e posterior confirmação do diagnóstico de uma doença dentro do espectro da neuromielite óptica, positiva para anticorpos anti-acuaporinas-4. O tratamento com esteroide e anticorpo monoclonal (Rituximab) levam a um controle adequado da doença.


Subject(s)
Humans , Neuromyelitis Optica , Vomiting , Brain , Magnetic Resonance Imaging , Aquaporins , Hiccup , Antibodies
4.
Rev. bras. ciênc. vet ; 29(1): 41-45, jan./mar. 2022. il.
Article in English | LILACS, VETINDEX | ID: biblio-1393235

ABSTRACT

This study aimed to promote the standardization of an indirect, enzyme-linked immunosorbent assay (ELISA) for the serological detection of B. anserina in Gallus gallusdomesticus. An aliquoted sera from vaccinated chicken with B. anserina antigen (GI), experimental infected chickens with B. anserina (GII) and rustic poultry rearing of G. gallus (GIII) were tested with in-house ELISA developed to detect serum antibodies against B. anserina in G. gallus domesticus. On average, the experimentally infected chickens became positive at 9 DPI a mean ± standard deviation (SD) ODI value of 163.11 ± 70.65. The highest observed Optical Density Index (ODI) was 372.54 ± 132.39, at 26 DPI, and the highest overall ODI value was 626.51. The vaccinated chickens became positive between 8 and 10 DPV, with an ODI of 245.59 at 10 DPV, with an overall maximum ODI of 543.13. A total of 108 blood samples were collected from poultry raised on rustic farms. Of the total samples collected, 58.33% (63/108) were considered positive for B. anserina. The maximum ODI found among these rustic chickens was 283.24. This stardardization provided a sensitivity and specificity of 100%.


Este estudo teve como objetivo promover a padronização de um ensaio imunoenzimático indireto (ELISA) para a detecção sorológica de Borrelia anserina em Gallus gallus domesticus. Um frango vacinado com antígeno de B. anserina (GI), frangos infectados experimentalmente com B. anserina (GII) e frangos criados de forma rústica (GIII) foram testados com ELISA indireto in house desenvolvido para a detecção sorológica contra B. anserina em G. gallus domesticus. Em média, os frangos infectados experimentalmente tornaram-se positivos aos 9º dia pós-inoculação (DPI), um valor do índice de densidade óptica (ODI) médio ± desvio padrão (SD) de 163,11 ± 70,65. O maior ODI observado foi 372,54 ± 132,39, em 26ºDPI, e o maior valor geral de ODI foi 626,51. Os frangos vacinados tornaram-se positivos entre 8º e 10° DPV, com um ODI de 245,59 a 10 DPV, com um ODI máximo geral de 543,13. Um total de 108 amostras de sangue foram coletadas de aves criadas em fazendas rústicas. Do total de amostras coletadas, 58,33% (63/108) foram consideradas positivas para B. anserina. O ODI máximo encontrado entre essas galinhas rústicas foi 283,24. Essa padronização proporcionou sensibilidade e especificidade de 100%.


Subject(s)
Animals , Poultry Diseases/diagnosis , Borrelia/immunology , Enzyme-Linked Immunosorbent Assay/veterinary , Serologic Tests/veterinary , Chickens/immunology , Antibodies/analysis
5.
Rev. bras. ciênc. vet ; 29(1): 64-66, jan./mar. 2022. il.
Article in English | LILACS, VETINDEX | ID: biblio-1395514

ABSTRACT

Trypanosoma vivax is considered the most important pathogenic Trypanosoma for cattle and causes great damage to the dairy and beef cattle industries. This study aimed to evaluate the prevalence of anti-T. vivax antibodies in dairy cattle from the municipality of Tapira, located in the Alto Paranaíba region, Minas Gerais, Brazil. The 74 blood serum samples from dairy cattle were analyzed using an indirect immunofluorescence reaction. The seroprevalence was 82.4 % (61/74), and the highest incidence observed can be correlated with the transit of untested animals, the presence of vectors, and needle sharing by owners. The data allowed defining Tapira as an area of expansion of T. vivax epizootic infections in the state of Minas Gerais.


O Trypanosoma vivax é considerado o mais importante trypanosoma patogênico para bovinos e causa grandes prejuízos na pecuária de corte e leite. Este estudo teve como objetivo avaliar a prevalência anticorpos de anti-Trypanosoma vivax em bovinos leiteiros do município de Tapira, localizado na região do Alto Paranaíba, Minas Gerais, Brasil. As 74 amostras de soro sanguíneo de bovinos leiteiros foram analisadas por meio de reação de imunofluorescência indireta. A soroprevalência foi de 82,4% (61/74), que pode estar relacionada ao trânsito de animais não testados, presença de vetores e compartilhamento de agulhas pelos proprietários. Os dados permitiram definir Tapira como uma área de expansão das infecções epizoóticas por Trypanosoma vivax no estado de Minas Gerais.


Subject(s)
Animals , Cattle , Trypanosomiasis, Bovine/diagnosis , Cattle Diseases/immunology , Seroepidemiologic Studies , Trypanosoma vivax , Fluorescent Antibody Technique, Indirect/veterinary , Antibodies/analysis
6.
Semina cienc. biol. saude ; 43(1): 119-128, jan./jun. 2022. ilus, tab
Article in English | LILACS | ID: biblio-1354464

ABSTRACT

Introduction: some plants such as turmeric, cinnamon, and okra are known to have therapeutic functions such as antioxidant and anti-inflammatory activity. Furthermore, an immunomodulatory role has been observed in the production of antibodies, in particular immunoglobulin A (IgA), which mediates a variety of protective functions for the organism. Objective: the aim of the present study was to investigate the effect of dietary plants on the production of IgA in healthy Wistar rats. Methods: thus, 48 male Wistar rats of 90 days of age were allocated to four groups. The animals were treated for 14 days with dried turmeric, cinnamon, or okra (50, 50, 12.5 mg/day, respectively) in phosphate buffered saline, or with only phosphate buffered saline by gavage. The animals received water and feed ad libitum. Body mass and relative weight ofperitoneal fat, adrenal gland, kidney, spleen, liver and thymus, biochemical parameters, and IgA levels were analyzed. Results: no significant changes were observed in the body mass, relative weight of organs and tissues, and biochemical parameters. An increase in serum IgA levels was observed in animals treated with turmeric or cinnamon. Conclusion: we conclude that the treatment with turmeric and cinnamon increased IgA production. Therefore, our study supports the idea that dietary supplementation with these plants may improve humoral immunity.


Introdução: algumas plantas como a cúrcuma, a canela e o quiabo são conhecidas por apresentar funções terapêuticas, como atividade antioxidante e anti-inflamatória. Além disso, tem sido observado um papel imunomodulador sobre a produção de anticorpos, em especial a imunoglobulina A (IgA), a qual medeia uma variedade de funções protetoras para o organismo. Objetivo: o objetivo do presente estudo foi investigar o efeito de plantas dietéticas na produção de IgA em ratos Wistar saudáveis. Métodos: destarte, 48 ratos machos Wistar com 90 dias de idade foram alocados em quatro grupos. Os animais foram tratados por 14 dias com cúrcuma seca, canela ou quiabo (50, 50, 12,5 mg/dia, respectivamente) em solução salina tamponada com fosfato ou apenas solução salina tamponada com fosfato, por gavagem. Os animais receberam água e ração ad libitum. Foram analisados a massa corporal e o peso relativo da gordura peritoneal, glândula adrenal, rim, baço, fígado e timo, parâmetros bioquímicos e níveis de IgA. Resultados: não foram observadas alterações significativas na massa corporal, no peso relativo dos órgãos e tecidos e nos parâmetros bioquímicos. Foi observado aumento dos níveis séricos de IgA nos animais tratados com cúrcuma ou canela. Conclusão: podemos concluir que o tratamento com cúrcuma e canela aumentou a produção de IgA. Portanto, nosso estudo suporta a ideia de que a suplementação alimentar com essas plantas pode melhorar a imunidade humoral.


Subject(s)
Rats , Spleen , Thymus Gland , Rats, Wistar , Abelmoschus , Curcuma , Kidney , Liver , Antibodies , Antibody Formation , Plants , Cinnamomum zeylanicum
7.
Article in Chinese | WPRIM | ID: wpr-941025

ABSTRACT

OBJECTIVE@#To express and purify the antigenic peptide of adeno-associated virus (AAV) capsid conserved regions in prokaryotic cells and prepare its rabbit polyclonal antibody.@*METHODS@#The DNA sequence encoding the conserved regions of AAV capsid protein was synthesized and cloned into the vector pET30a to obtain the plasmid pET30a-AAV-CR for prokaryotic expression and purification of the conserved peptides. Coomassie blue staining and Western blotting were used to identify the AAV conserved peptides. Japanese big ear white rabbits were immunized with AAV conserved region protein to prepare polyclonal antibody, with the rabbits injected with PBS as the control group. The antibody titer was determined with ELISA, and the performance of the antibody for recognizing capsid protein sequences of AAV1-AAV10 was assessed with Western blotting and immunofluorescence assay.@*RESULTS@#The plasmid pET30a-AAV-CR was successfully constructed, and a recombinant protein with a relative molecular mass of 17000 was obtained. The purified protein induced the production of antibodies against the conserved regions of AAV capsid in rabbits, and the titer of the purified antibodies reached 1:320 000. The antibodies were capable of recognizing a wide range of capsid protein sequences of AAV1-AAV10.@*CONCLUSION@#We successfully obtained the polyclonal antibodies against AAV capsid conserved region protein from rabbits, which facilitate future studies of AAV vector development and the biological functions of AAV.


Subject(s)
Animals , Antibodies , Capsid , Capsid Proteins/genetics , Dependovirus/genetics , Prokaryotic Cells , Rabbits , Recombinant Proteins/genetics
8.
Article in Chinese | WPRIM | ID: wpr-936069

ABSTRACT

Objective: To construct a prediction model of pathologic complete response (pCR) in locally advanced rectal cancer patients who received programmed cell death protein-1 (PD-1) antibody and total neoadjuvant chemoradiotherapy by using radiomics based on MR imaging data and to investigate its predictive value. Methods: A clinical diagnostic test study was carried out. Clinicopathalogical and radiological data of 38 patients with middle-low rectal cancer who received PD-1 antibody combined with total neoadjuvant chemoradiotherapy and underwent TME surgery from January 2019 to September 2021 in our hospital were retrospectively collected. Among 38 patients, 23 were males and 15 were females with a median age of 68 (47-79) years and 13 (34.2%) a chieved pCR. These 38 patients were stratified and randomly divided into the training group (n=26) and test group (n=12) for modeling. All the patients underwent rectal MRI before treatment. The clinical, imaging and radiomics features of all the patients were collected, and the clinical feature model and radiomics model were constructed. The receiver operating characteristic (ROC) curves of each model were drawn, and the constructed model was evaluated through the area under the curve (AUC), accuracy, sensitivity, specificity, positive predictive value and negative predictive value. Results: There were no significant differences in age, gender, primary location of tumor and postoperative pathology between the two groups (all P>0.05). Forty-one features were extracted from region of interest in each modality, including 9 first-order features, 24 gray level co-occurrence matrix features and 8 shape features. From 38 patients, 41 features were extracted from each imaging modality of baseline and preoperative DWI and T2WI images, totally 164 features. Only 4 features were preserved after correlation analysis between each pair of features and t-test between pCR and non-pCR subjects. After LASSO cross validation, only the first-order skewness of the baseline DWI image before treatment and the volume in the baseline T2WI image before treatment were retained. The area under the curve, sensitivity, specificity, positive and negative predictive values of the prediction model established by applying these two features in the training group and the test group were 0.856 and 0.844, 77.8% and 100.0%, 88.2% and 75.0%, 77.8% and 66.7%, 88.2% and 100.0%, respectively. The decision curve analysis of the radiomics model showed that the strategy of this model in predicting pCR was better than that in treating all the patients as pCR and that in treating all the patients as non-pCR. Conclusion: The pCR prediction model for rectal cancer patients receiving PD-1 antibody combined with total neoadjuvant radiochemotherapy based on MRI radiomics has the potential to be used in clinical screening or rectal cancer patients who can be spared from radical surgery.


Subject(s)
Aged , Antibodies/therapeutic use , Female , Humans , Magnetic Resonance Imaging/methods , Male , Middle Aged , Neoadjuvant Therapy/methods , Programmed Cell Death 1 Receptor , Rectal Neoplasms/therapy , Retrospective Studies
9.
Repert. med. cir ; 31(2): 182-186, 2022. ilus., tab.
Article in English, Spanish | LILACS, COLNAL | ID: biblio-1390627

ABSTRACT

Introducción: la neuritis óptica requiere un diagnóstico y manejo desafiante, se puede presentar de forma aislada o asociada con trastornos inflamatorios, siendo hasta 25% de la clínica de los síndromes desmielinizantes agudos pediátricos. La incidencia anual estimada de neuritis óptica pediátrica es 0.2 por 100.000 niños, con una preponderancia femenina y una edad media de presentación de 9 a 11 años. Presentación del caso: paciente de 12 años con cuadro agudo de diplopía, dolor ocular izquierdo sin antecedentes relevantes, en quien se encontró parálisis del VI par izquierdo y resonancia magnética cerebral (RMc) con realce en nervio óptico, iniciándose tratamiento con corticoterapia endovenosa, previo descarte de patologías infecciosas, con evolución satisfactoria. Conclusiones: en el espectro de la neuritis óptica es importante el conocimiento de sus diferentes etiologías, debido a que el tratamiento y pronóstico dependen de la causa.


Introduction: optic neuritis (ON) requires a challenging diagnosis and management. It can appear as an isolated condition or in association with inflammatory disorders, being 25% of the clinical manifestations of pediatric acute demyelinating syndromes. The estimated annual incidence of pediatric ON is of 0.2 per 100.000, with a female preponderance and a mean age at onset of 9 to 11 years. Case report: a 12-year-old patient presenting with acute diplopia and left ocular pain, referring no relevant past history. Left sixth nerve palsy and brain magnetic resonance imaging (MRI) showing optic nerve enhancement, were evidenced. Therapy with intravenous corticosteroids was started, once an infectious etiology had been ruled out, with satisfactory progression. Conclusions: in the optic neuritis spectrum disorders, knowledge regarding other potential etiologies is key to treatment and prognosis, which depend on the cause.


Subject(s)
Humans , Female , Child , Optic Neuritis , Myelitis , Antibodies , Multiple Sclerosis
10.
NOVA publ. cient ; 20(38): [25], 2022 enero-junio. gráficos, mapas, tablas e ilustraciones
Article in Spanish | LILACS | ID: biblio-1396942

ABSTRACT

Borrelia burgdorferi, is the spirochete responsible for causing Lyme disease in man and different animals. Objective. Detect specific IgG type antibodies against Borrelia burgdorferi, in canines using the immunofluorescence technique and its correlation with other factors associated with Lyme disease. Methods. Blood was taken for IgG detection against Borrelia burgdorferi sl; Peripheral blood smear of the canines and hemolymph of the ticks to search for spirochetes with Wright staining and finally classification of the ticks using morphometric keys. Results. In the serological test, on average 69.0% of the canines sampled gave positive results in the different titrations. Bacterial structures were spirally visualized both in the peripheral blood of the canines and in the hemolymph of the ticks. The vector found was classified as Rhipicephalus sanguineus, until now not reported in the scientific literature as a carrier of Borrelia, nor associated with the disease.


Subject(s)
Humans , Borrelia , Lyme Disease , Bacterial Structures , Antibodies
11.
Biomédica (Bogotá) ; 41(4): 745-755, oct.-dic. 2021. tab, graf
Article in English | LILACS | ID: biblio-1355747

ABSTRACT

Abstract | Introduction: Enteric viruses have been associated with the production of a variety of diseases transmitted by the fecal-oral route and carried through contaminated food and water. Given their structure and composition, they are highly resistant to environmental conditions and most of the chemical agents used in the purification processes. Therefore, the systematic monitoring of raw water is necessary to ensure its quality especially when it is used for producing drinking water for human consumption. Objective: We identified the presence of rotavirus and hepatitis A virus by means of the fluoro-immuno-magnetic separation technique (FIMS) in raw water taken from four purification plants and their water supplies in the department of Norte de Santander. Materials and methods: The viruses were captured and separated from the water samples using magnetic microparticles functionalized with monoclonal anti-Hepatitis A and anti-Rotavirus antibodies. Confocal microscopy was used to monitor the viral concentration process and transmission electron microscopy for the morphological visualization of the separated viruses. The reverse transcriptase-coupled polymerase chain reaction (RT-PCR) was applied to confirm the presence of pathogens. Results: The two enteric viruses were identified in the majority of the analyzed water samples including water supply sources. Conclusion: We determined that the FIMS technique together with RT-PCR is highly effective for the detection of viral pathogens in complex matrices such as raw water.


Resumen | Introducción. Los virus entéricos se asocian con una serie de enfermedades transmitidas por vía fecal-oral en alimentos o agua contaminada. Dada su estructura y composición, son muy resistentes a las condiciones ambientales y a la mayoría de los agentes químicos empleados en los procesos de potabilización, por lo cual es necesario un monitoreo sistemático del agua cruda para asegurar su calidad, máxime cuando se emplea como materia prima en la producción de agua potable para consumo humano. Objetivo. Determinar la presencia de rotavirus y del virus de la hepatitis A mediante la técnica de separación fluoro-inmuno-magnética en agua cruda procedente de cuatro plantas de potabilización y sus fuentes hídricas en el departamento de Norte de Santander. Materiales y métodos. Los virus fueron capturados y separados a partir de las muestras de agua, empleando micropartículas magnéticas funcionalizadas con anticuerpos monoclonales anti-hepatitis A y anti-rotavirus. Se empleó microscopía confocal para hacer el seguimiento del proceso de concentración viral y, microscopía electrónica de transmisión, para la visualización morfológica de los virus separados. La reacción en cadena de la polimerasa acoplada a transcriptasa inversa (RT-PCR) se utilizó para confirmar la presencia de los patógenos. Resultados. Los dos virus entéricos se detectaron en la mayoría de las muestras de agua analizadas, incluidas las de sus fuentes hídricas. Conclusión. La técnica de separación fluoro-inmuno-magnética acoplada a RT-PCR fue altamente efectiva en la detección de patógenos virales en matrices complejas como el agua cruda.


Subject(s)
Rotavirus Infections , Raw Water , Magnetic Segregation , Water Purification , Hepatitis A , Antibodies
12.
Rev. Méd. Inst. Mex. Seguro Soc ; 59(5): 412-416, oct. 2021. tab
Article in Spanish | LILACS | ID: biblio-1357981

ABSTRACT

Introducción: los problemas de salud mental derivados de la pandemia por COVID-19, entre ellos la depresión, la ansiedad y el estrés, requieren identificación. Objetivo: validar y adaptar la COVID-19 Anxiety Scale (CAS) al español. Material y métodos: se realizó un estudio transversal en un total de 303 individuos, en los cuales se aplicó la CAS y se identificaron de forma secundaria, como comparación, pruebas de anticuerpos para SARS-CoV-2. Resultados: se hizo el proceso de validación transcultural recomendado por la American Academy of Orthopeadic Surgeon (AAOS) y se obtuvo un alfa de Cronbach de 0.8493 (covarianza promedio interitem de 0.2620). Conclusiones: se logró una versión al español de la CAS válida para utilizarla en la práctica clínica rutinaria.


Background: Mental health problems derived from the COVID-19 pandemic, including depression, anxiety, and stress, need to be identified. Objective: To validate and adapt the COVID-19 Anxiety Scale (CAS) into Spanish. Material and methods: A cross-sectional study was carried out in a total of 303 individuals who answered the Covid-19 Anxiety Scale. Antibody tests for SARS-CoV-2 were identified as a secondary comparison. Results: It was carried out the cross-cultural validation process recommended by the American Academy of Orthopeadic Surgeon (AAOS). It was obtained a Cronbach's alpha of 0.8493 (with an average interitem covariance of 0.2620). Conclusions: It was achieved a valid Spanish version of CAS to be used in routine clinical practice.


Subject(s)
Humans , Male , Female , Anxiety , Mental Health , Validation Study , Depression , SARS-CoV-2 , COVID-19 , Cross-Sectional Studies , Adaptation to Disasters , Health Facilities , Mexico , Antibodies
13.
Rev. chil. enferm. respir ; 37(3): 244-249, sept. 2021. tab
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1388153

ABSTRACT

Resumen Los métodos diagnósticos clásicos para la tuberculosis son de baja sensibilidad o son muy lentos en la obtención de resultados (baciloscopía, cultivo de Koch). De ahí nace la necesidad de nuevos métodos diagnósticos para esta enfermedad. Los biomarcadores surgen como una opción a esta problemática, con un buen rendimiento diagnóstico, costo y accesibilidad. Ellos permiten identificar la respuesta inflamatoria y/o metabólica del huésped, extrapolando la presencia de Mycobacterium tuberculosis; o identifican moléculas propias del patógeno. En la presente revisión se describen biomarcadores que presentan un buen rendimiento diagnóstico basados en metodologías de investigación de alto nivel (estudio de cohortes, prospectivos, muestreo consecutivo o aleatorizado, comparación de rendimiento diagnóstico frente a cultivo). Es necesario el desarrollo de estas nuevas técnicas con el fin de realizar el diagnóstico precoz de la enfermedad y lograr así su tan ansiada eliminación.


The classical laboratory diagnostic methods for tuberculosis have a low sensitivity or take a long time to know their results. New methods are underway. Biomarkers are a good option to improve our diagnostic approach to this disease. They have good performance, low cost and accessibility. They identify a patient's inflammatory or metabolic response to Mycobacterium Tuberculosis or identifies molecules that are typical of the pathogen. In this paper we sum up the biomarkers with a good diag-nostic performance described in well design investigations. Early diagnosis with these new techniques should contribute to the elimination of the disease.


Subject(s)
Humans , Tuberculosis/diagnosis , Biomarkers/analysis , RNA/analysis , Proteins/analysis , Cytokines/analysis , Sensitivity and Specificity , Antibodies/analysis , Mycobacterium tuberculosis/isolation & purification , Mycolic Acids/analysis
14.
Rev. enferm. neurol ; 20(3): 220-230, sep.-dic. 2021. tab
Article in Spanish | LILACS, BDENF | ID: biblio-1372943

ABSTRACT

Introducción: este caso describe el abordaje y aplicación de intervenciones especializadas de enfermería en una mujer de 49 años con encefalitis autoinmune teniendo como base el modelo de Dorothea Orem. Objetivo: identificar los requisitos universales alterados en la persona para restablecerlos mediante acciones de cuidado. Material y métodos: tras la selección de la persona y autorización del cuidador primario se aplicó un instrumento de valoración que permitió realizar diagnósticos utilizando el formato PESS, planeación y ejecución de intervenciones de enfermería, así como evaluación de cada una, por último, la elaboración de un plan de alta. Descripción del caso clínico: mujer de 49 años presentó súbitamente cefalea, alteración del lenguaje, agitación, inatención y labilidad emocional, punción lumbar arrojó leve proceso inflamatorio, descartando etiología vascular e infecciosa sospechando de autoinmunidad. Relevancia: analizar diferentes situaciones clínicas permitiendo plantear soluciones y estrategias para la mejora en el cuidado enfermero. Resultados y conclusiones: resultados satisfactorios, la evolución fue poco a poco, con grandes cambios que le permitieron ser funcional en las actividades de la vida diaria; ahora, a un año de su enfermedad a pesar del deterioro cognitivo puede deambular y alimentarse por sí misma.


Introduction: This case study describes the approach and application of specialized nursing interventions applied to a 49-year-old woman with autoimmune encephalitis based on the Dorothea Orem model. Objective: Identify the universal requirements altered in the person to restore them through care actions. Methodology: After the selection of the person and authorization of the primary caregiver, an assessment instrument was applied that allowed diagnoses to be made using the PESS format, planning and execution of nursing interventions, as well as the evaluation of each one, ending with the elaboration of a high plan. Description of the clinical case: a 49-year-old woman who suddenly presented headache, language alteration, agitation, inattention and emotional lability, lumbar puncture showed a mild inflammatory process, ruling out vascular and infectious etiology, suspecting autoimmunity. Relevance: The case study analyzes different clinical situations allowing solutions and strategies to be proposed to improve nursing care. Results and conclusions: The results were satisfactory, the evolution of the person was slow but with great changes that allowed her to be functional in the activities of daily life, currently one year after his illness, despite the cognitive deterioration, she can walk and feed herself by herself.


Subject(s)
Humans , Female , Middle Aged , Nursing Care , Plasmapheresis , Receptors, N-Methyl-D-Aspartate , Encephalitis , Immunotherapy , Antibodies
15.
Infectio ; 25(3): 169-175, jul.-set. 2021. tab
Article in Spanish | LILACS, COLNAL | ID: biblio-1250088

ABSTRACT

Resumen Objetivo: Verificación del desempeño de las pruebas serológicas rápidas utilizadas en el departamento de Risaralda, Colombia. Métodos: Estudio analitico, de corte transversal. Incluyó muestras de sueros de trabajadores de la salud de la ciudad de Pereira, quienes tuvieron sospecha clínica y epidemiológica por SARS-CoV-2. El procesamiento y validación de las pruebas fue realizado en las instalaciones de la Universidad Tecnológica de Pereira. Se calculó sensibilidad y especificidad de las pruebas rápidas serológicas IgM/IgG usando como prueba de oro la RT-PCR. Resultados: Se incluyeron las muestras de 144 profesionales de la salud. Las pruebas serológicas rápidas evidenciaron ser útiles para identificar o descartar la presencia de anticuerpos IgM e IgG, especialmente en pacientes sintomáticos, en quienes el inicio de los síntomas es superior a 11 días. Discusión: El uso de pruebas rápidas se encuentra en aumento, no solo por la rapidez de sus resultados, sino también por los bajos costos asociados y la necesidad de identificar pacientes no susceptibles, quienes deben priorizar su retorno a actividades laborales en comunidad como parte de la reactivación económica de Colombia. Es necesario confirmar el desempeño de la prueba para aumentar la probabilidad de una adecuada clasificación antes de proceder a su uso rutinario.


Abstract Objective: We aimed to realize a verification of the performance of the rapid serological tests used in Risaralda department. Methods: Analytical, cross-sectional study. Serum samples from health workers in Pereira city, who had a clinical and epidemiological suspicion for SARS-CoV-2 were included. The processing and validation of the tests was carried out at Universidad Tecnológica de Pereira. Sensitivity and specificity of rapid IgM / IgG sero logical tests were calculated using RT-PCR as the gold standard test. Results: 144 samples of health professionals were included. Rapid serological tests useful to identify or rule out the presence of IgM and IgG antibodies, especially in symptomatic patients, in whom the onset of symptoms is longer than 11 days. Discussion: The use of rapid tests is increasing, not only due to the speed of their results, but also due to the low associated costs and the need to identify non-susceptible patients, who must prioritize their return to work activities in the community as part of the economic reactivation of Colombia. It is necessary to confirm the adequate performance of the test to increase the probability of an adequate classification before proceeding with the routine use of this test.


Subject(s)
Humans , Male , Female , Adult , Serologic Tests , Health Personnel , SARS-CoV-2 , Cross-Sectional Studies , COVID-19/diagnosis , Antibodies , Occupational Groups , Antigens
17.
Infectio ; 25(2): 114-119, abr.-jun. 2021. tab
Article in Spanish | LILACS, COLNAL | ID: biblio-1250077

ABSTRACT

Resumen Objetivo: Determinar la prevalencia de sífilis, hepatitis B y virus de la inmunodeficiencia humana en una población privada de la libertad de un establecimiento carcelario masculino de Bogotá D.C.-Colombia en 2019. Materiales y métodos: Se realizó un estudio de corte transversal en un establecimiento carcelario masculino de Bogotá, se incluyeron personas privadas de la libertad, mayores de 18 años. Los sujetos fueron sometidos a pruebas de detección de anticuerpos contra el Treponema pallidum, Antígenos de Superficie contra hepatitis B (HBsAg) y Virus de Inmunodeficiencia Humana (VIH) y respondieron un cuestionario estructurado para la descripción de conductas de riesgo. Resultados: Participaron 447 sujetos, ubicados en 7 pabellones del establecimiento carcelario. La prevalencia de sífilis fue del 5.8% (IC95% 3.8 - 8.4), del 1.1% para VIH (IC95% 0.4 - 2.6), y del 0.45% para hepatitis B crónica (IC95% 0.05 - 1.6). Discusión: A pesar de que la prevalencia documentada para estas patologías es más alta que en la población general, los resultados son más bajos que los reporta dos en instituciones de condiciones similares en otras latitudes. Se recomienda que el establecimiento continúe desarrollando políticas de promoción y prevención de estas patologías dentro de su población.


Abstract Objective: To determine the prevalence of syphilis, hepatitis B and the human immunodeficiency virus (HIV) in the male prison population in Bogotá, Colombia in 2019. Materials and methods: A cross-sectional study was carried out in a male prison center in Bogotá, in which sequential sampling, stratified by ward, included people deprived of liberty, over 18 years of age and who voluntarily agreed to participate in the investigation. Subjects underwent tests for antibodies to Treponema pallidum, Surface Antigens against hepatitis B (HBsAg) and Human Immunodeficiency Virus (HIV) and they answered a structured questionnaire for the description of risk behaviors. Results: A total of 447 subjects were included, belonging to 7 prison wards. The prevalence of syphilis was 5.8% (95% CI 3.8 - 8.4), 0.5% for chronic hepatitis B (95% CI 0.05 - 1.6) and 1.1% for HIV (95% CI 0.4 - 2.6). Discussion: Although the documented prevalence for these pathologies is higher than in the general population, the results are lower than those reported in other institutions with similar conditions in other latitudes. It is recommended that the institution continue to strengthen its policies for the promotion and prevention of these pathologies within its population.


Subject(s)
Humans , Male , Adult , Middle Aged , Syphilis , Prevalence , HIV , Hepatitis B , Prisons , Colombia , Policy , Antibodies , Antigens, Surface
18.
Biomédica (Bogotá) ; 41(supl.1): 113-120, mayo 2021. tab
Article in Spanish | LILACS | ID: biblio-1285453

ABSTRACT

Resumen | Introducción. La mayoría de las personas con enfermedad de Chagas desarrolla anticuerpos específicos contra Trypanosoma cruzi. En la infección temprana se producen anticuerpos IgM contra T. cruzi que son reemplazados por IgG durante el curso de la enfermedad. Los primeros síntomas de la enfermedad suelen ser muy leves y atípicos, por lo que a menudo no se detecta en la fase aguda. Objetivos. Evaluar la sensibilidad y la especificidad clínica y analítica, la precisión y la eficacia del UMELISA CHAGAS® con la incorporación de nuevos péptidos sintéticos en la fase sólida representativos de la proteína SAPA (Shed Acute Phase Antigen) y del antígeno TSA (Trypomastigote Surface Antigen). Materiales y métodos. Se evaluó un panel de desempeño de título mixto anti-I cruzi y uno de seroconversión de Chagas, así como muestras de suero positivas y negativas provenientes de zonas endémicas de la enfermedad y muestras positivas de otras enfermedades que podían interferir con la prueba. Las pruebas Bioelisa CHAGAS, Chagatest ELISA recombinante v. 4.0, Chagatest HAI y SD BIOLINE CHAGAS Ab Rapid, se emplearon como referencia. Resultados. Los porcentajes de sensibilidad y especificidad clínica fueron de 97,73 % (IC95% 96,23-99,24) y 99,33 % (IC95% 98,88-99,78), respectivamente. Se obtuvo un 98,96 % de eficacia y una buena precisión. Conclusiones. Los resultados demuestran que la nueva fase sólida del UMELISA CHAGAS® puede utilizarse para el inmunodiagnóstico, la certificación de sangre y la vigilancia epidemiológica en países endémicos y no endémicos con población de alto riesgo.


Abstract | Introduction: Most people with Chagas disease develop specific antibodies against Trypanosoma cruzi. In early infection, IgM antibodies against T. cruzi are produced and later replaced for IgG antibodies during the course of the disease. The first symptoms of the infection may be very mild and atypical, which is why the disease is often not detected in the acute phase. Objectives: To evaluate the clinical and analytical sensitivity, and specificity, accuracy, and efficacy of UMELISA CHAGAS™ with the addition of new synthetic peptides in the solid phase representative of the shed acute phase antigen protein (SAPA) and the trypomastigote surface antigen (TSA). Materials and methods: We evaluated a mixed anti-T. cruzi titer performance panel and a Chagas seroconversion one, as well as positive and negative serum samples from endemic areas of the disease and positive samples for other diseases that may interfere with the assay. The Bioelisa CHAGAS assay, Chaga test recombinant ELISA v.4.0, Chagatest HAI, and SD BIOLINE CHAGAS Ab Rapid were used as reference tests. Results: The sensitivity of the assay was 97.73% (95% CI: 96,23-99,24) and the clinical specificity, 99.33% (95% CI: 98,88-99,78) while the efficacy and the accuracy were 98.96%. Conclusions: Our results show that the new solid phase of UMELISA CHAGAS® can be used for immunodiagnostic, blood certification, and epidemiological surveillance in endemic and non-endemic countries with high-risk populations.


Subject(s)
Chagas Disease/diagnosis , Peptides , Trypanosoma cruzi , Antibodies
19.
Mem. Inst. Invest. Cienc. Salud (Impr.) ; 19(1)abr. 2021. tab, ilus
Article in Spanish | LILACS, BDNPAR | ID: biblio-1337691

ABSTRACT

El embarazo es la única causa natural de inmunización contra el sistema de Antígenos Leucocitarios Humano (HLA). Durante la gestación hay paso de leucocitos fetales a través de la placenta, lo que puede desencadenar en la madre una respuesta inmunológica contra los antígenos HLA fetales de origen paterno, con la consecuente producción de anticuerpos. El objetivo del estudio fue conocer la prevalencia de sensibilización a antígenos HLA inducida por embarazos en mujeres paraguayas y estudiar las características y especificidades de los anticuerpos encontrados. Realizamos un estudio descriptivo, prospectivo, de corte transversal de 319 mujeres paraguayas, que acudieron al Laboratorio Central de Salud Pública entre abril de 2017 y abril de 2018 utilizando la tecnología LUMINEX para la detección de anticuerpos anti- HLA. Se encontraron anticuerpos anti-HLA en 46% de las mujeres multíparas. Se detectaron anticuerpos contra todos los antígenos testados. La gran mayoría de los sueros resultaron ser poliespecíficos. Concluimos que al aumentar el número de gestas no solo aumenta la probabilidad de una mujer de desarrollar anticuerpos anti- HLA, sino que también parece aumentar la cantidad de especificidades desarrolladas y el título de los anticuerpos


Pregnancy is the only natural cause of immunization against the Human Leukocyte Antigen (HLA) system. During pregnancy, fetal leukocytes pass through the placenta, which can trigger an immunological response in the mother against the fetus paternal HLA antigens, with the consequent production of antibodies. The objective of this study was to determine the prevalence of pregnancy-induced HLA antigen sensitization in Paraguayan women and to study the characteristics and specificities of the antibodies found. We conducted a descriptive, prospective, cross-sectional study of 319 Paraguayan women, who attended the Central Laboratory of Public Health between April 2017 and April 2018 using LUMINEX technology to detect anti-HLA antibodies. We found anti-HLA antibodies in 46% of multiparous women. Antibodies against all tested antigens were detected. The vast majority of the sera exhibited multiple specificities. We conclude that increasing the number of gestations not only increases a woman's likelihood of developing anti-HLA antibodies, but it also appears to increase the number of developed specificities and titers of antibodies


Subject(s)
Humans , Female , Pregnancy , Adult , HLA Antigens , Immunity , Antibodies , Pregnancy , Prevalence
20.
Rev. argent. reumatolg. (En línea) ; 32(1): 16-20, mar. 2021. ilus, tab
Article in Spanish | LILACS, BINACIS | ID: biblio-1279754

ABSTRACT

Introducción: El interferón (IFN) tipo I es una citoquina que juega un rol fundamental en la patogenia del Lupus Eritematoso Sistémico (LES). Diferentes niveles de esta citoquina podrían explicar la heterogeneidad de esta patología y ser útil para evaluar la actividad de la misma. Objetivos: Determinar los niveles de IFN tipo I sérico en pacientes con LES y evaluar su utilidad como biomarcador de actividad. Material y Métodos: 16 pacientes con LES (ACR 1997) y 16 controles. Métodos: Actividad de la enfermedad (SLEDAI-2K), daño orgánico (SLICC), IFN tipo I (HEK-Blue-IFNα/β), anticuerpos anti-DNAdc (Inmunofluorescencia Indirecta), anticuerpos anti-ENA (ELISA), C3-C4 (Inmunoturbidimetría). Estadística: InfoStat/Instat/MedCalc. Valores de p<0,05 fueron considerados estadísticamente significativos. Resultados: Se observó un aumento de la concentración de IFN en el grupo LES con respecto al control (p<0,05). Los pacientes con valores de IFN superiores al punto de corte, se asociaron con la presencia de anticuerpos anti-DNAdc (OR:13,33; p<0,05). Pacientes con hipocomplementemia y aquellos con puntaje de SLEDAI-2K mayor a 8 presentaron mayores niveles de IFN comparados con pacientes con complemento normal y menor puntaje de índice, respectivamente (p<0,05). Conclusiones: Estos resultados sugieren la importancia que podría tener la determinación de IFN tipo I para el monitoreo de la actividad del LES.


Introduction: Type I interferon (IFN) is a cytokine that plays a fundamental role in the pathogenesis of Systemic Lupus Erythematosus (SLE). Different levels of this cytokine could explain the heterogeneity of this pathology and be useful to evaluate its activity. Objectives: To determine the serum type I IFN levels in patients with SLE and evaluate its usefulness as a biomarker of activity. Material and Method: 16 patients with SLE (ACR 1997) and 16 controls. Methods: Disease activity (SLEDAI-2K), organ damage (SLICC), type I IFN (HEK-Blue-IFNα/β), anti-dsDNA antibodies (Indirect Immunofluorescence), anti-ENA antibodies (ELISA), C3-C4 (Immunoturbidimetry). Statistics: InfoStat/Instat/MedCalc. P values <0.05 were statistically significant. Results: An increase in IFN concentration was observed in the SLE group respect to the control (p <0.05). Patients with IFN values above the cut-off point were associated with the presence of anti-dsDNA antibodies (OR: 13.33; p<0.05). Hypocomplementemic patients and those with a SLEDAI-2K score greater than 8 had higher IFN levels compared to patients with normal complement and a lower index score, respectively (p<0.05). Conclusions: These results suggest the importance that the determination of IFN type I could have for the monitoring of SLE activity.


Subject(s)
Humans , Lupus Erythematosus, Systemic , Interferon Type I , Antibodies
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