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1.
Rev. cir. (Impr.) ; 74(4): 426-431, ago. 2022. ilus
Article in Spanish | LILACS-Express | LILACS | ID: biblio-1407931

ABSTRACT

Resumen El verde de indocianina es un tinte que se ha utilizado en medicina durante varias décadas. Tiene una serie de aplicaciones, incluida la cirugía reconstructiva y las quemaduras. Permite detectar áreas de tejido con perfusión reducida, lo que reduce el riesgo de complicaciones posoperatorias en forma de procesos de cicatrización alterados y necrosis. La técnica de imágenes que utiliza este tinte, permite observar los cambios en la fluorescencia en tiempo real y que, se ha demostrado, ocurren entre las capas superficiales y profundas en las quemaduras. Esto permite un diagnóstico cualitativo y cuantitativo de la profundidad de la quemadura, lo que se traduce en la elección de un tratamiento adicional. Se aprecia la importancia particular de este método en la prevención de la necrosis cutánea con el complejo areola-pezón durante la reconstrucción mamaria simultánea. Se necesitan más ensayos controlados aleatorios prospectivos para considerarlo el "método de elección" en la práctica clínica.


Indocyanine green is a dye that has been used in medicine for several decades. It has a number of applications, including reconstructive surgery and burns. It allows the detection of areas of tissue with reduced perfusion, which reduces the risk of postoperative complications in the form of altered healing processes and necrosis. The imaging technique that uses this dye allows us to observe the changes in fluorescence in real time that have been shown to occur between the superficial and deep layers in burns. This allows a qualitative and quantitative diagnosis of the depth of the burn, which results in the choice of additional treatment. The particular importance of this method in the prevention of skin necrosis with the areolanipple complex is appreciate during simultaneous breast reconstruction. More prospective randomized controlled trials are needed to consider it the 'method of choice' in clinical practice.


Subject(s)
Humans , Burns/diagnosis , Coloring Agents/therapeutic use , Indocyanine Green/therapeutic use , Wound Healing , Fluorescence , Mastectomy
2.
Arq. odontol ; 58: 63-86, 2022. tab, graf, ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1380554

ABSTRACT

Objetivo: O objetivo dessa revisão sistemática e meta-análise foi avaliar a acurácia de dispositivos de indução de fluorescência e do método radiográfico para diagnóstico da cárie dentária em dentes decíduos. Métodos: Buscas no PubMed, Web of Science, Scopus, Embase, Open Grey e Google Scholar foram realizadas em agosto de 2021. Referências que preenchiam os critérios de elegibilidade foram incluídas. O risco de viés dos artigos incluídos foi avaliado com a ferramenta QUADAS-2. Foram realizadas meta-analises com o MedCalc. Resultados fornecidos em área sob a curva (ASC), intervalo de confiança (IC) e erro padrão (EP). Resultados: Títulos/resumos de 619 referências foram avaliados por dois revisores calibrados de forma independente. Cinquenta e seis artigos foram selecionados para leitura do texto completo. Onze artigos foram incluídos na revisão-sistemática e meta-análise. Os artigos incluídos apresentaram baixo risco de viés. A ASC para diagnóstico de cárie em esmalte com o laser DIAGNOdent foi de 75,8% (IC = 68,1% - 83,6% / EP = 3,9% / I2 = 67,95%) e para o diagnóstico de cárie em dentina foi de 80,6% (ASC = 80,6% / IC = 65,7% - 95,6% / EP = 7,6% / I2 = 89,24%). A ASC para o diagnóstico de cárie em esmalte com a radiografia interproximal foi de 61,7% (IC = 51,4% - 72,1% / EP = 5,3% / I2 = 44,51%) e a ASC para diagnóstico de cárie em dentina com a radiografia interproximal foi de 73,7% (IC = 45,1% - 100% / EP = 14,6% / I2 = 96,97%). Conclusão: O método radiográfico e os dispositivos de indução de fluorescência são boas opções para diagnóstico de cárie em dentes decíduos. Ambos apresentam melhores resultados em lesões em dentina. Os métodos que utilizam fluorescência tiveram melhores resultados em esmalte quando comparados à radiografia.


Aim: This systematic review and meta-analysis aimed to evaluate the accuracy of the fluorescence induction devices and radiographic method for diagnosing dental caries in primary teeth. Methods: Searches in PubMed, Web of Science, Scopus, Embase, Open Grey, and Google Scholar were conducted in August 2021. References that met the eligibility criteria were included. The risk of bias of the included articles was assessed with QUADAS-2. Meta-analyses were performed with MedCalc. Results were provided as area under the curve (AUC), confidence interval (CI), and standard error (SE). Results: Titles/abstracts of 619 references were evaluated by two trained reviewers independently. Fifty-six articles were selected for full-text reading. Eleven articles were included in the systematic review and meta-analysis. The included articles showed a low risk of bias. The AUC for the diagnosis of caries on enamel with the DIAGNOdent laser was 75.8% (CI = 68.1% - 83.6% / SE = 3.9% / I2 = 67.95%), whereas for the diagnosis of caries on dentin, it was 80.6% (AUC = 80.6% / CI = 65.7% - 95.6% / SE = 7.6% / I2 = 89.24%). The AUC for the diagnosis of enamel caries with the interproximal radiograph was 61.7% (CI = 51.4% - 72.1% / SE = 5.3% / I2 = 44.51%) and the AUC for the diagnosis of dentin caries with the interproximal radiograph was 73.7% (CI = 45.1% - 100% / SE = 14.6% / I2 = 96.97%). Conclusion: Radiography and fluorescence devices are useful tools for the diagnosis of caries in deciduous teeth. Both showed better results in dentin lesions. The methods using fluorescence had better results in enamel when compared to radiography.


Subject(s)
Radiography , Dental Caries , Fluorescence , Systematic Review
3.
Braz. J. Pharm. Sci. (Online) ; 58: e19692, 2022. graf
Article in English | LILACS | ID: biblio-1384014

ABSTRACT

Abstract The development of stable cell lines producing recombinant proteins is very time-consuming and laborious. One of the practical approaches successfully performed is Fluorescence-Activated Cell Sorting (FACS). A mutated chimeric tissue plasminogen activator (mt-PA) was developed by removing the first three domains of t-PA, insertion of GHRP sequence and mutation toward resistance to plasminogen activator inhibitor-1 (PAI-1). In the current study, a new stable CHO-DG44 cell line producing mt-PA was developed by two sequential clonal selections: FACS and clonal-selection by limiting dilution. Furthermore, the expression was more evaluated using two different expression media. Finally, the high-producing clones were selected based on the dot blot and amidolytic activity test. The transfection efficiency of CHO-DG44 cells was 38% as measured by flow cytometry on green fluorescent protein (GFP). After performing FACS on stable cell pools, the expression yield was increased to fifty-fold. In terms of growth profile, CD-DG44 showed higher viability and cell density results than ProCHO5 medium. The expression of mt-PA was significantly higher in CD-DG44 than in ProCHO5, 765 and 280 IU/mL, respectively. Our data indicated that selection of an appropriate expression medium played a critical role in the development of potent producing stable cells by FACS.


Subject(s)
Tissue Plasminogen Activator , Process Optimization , Flow Cytometry/methods , Fluorescence , Cell Count/instrumentation , Clone Cells/classification , Plasminogen Activator Inhibitor 1/adverse effects , Green Fluorescent Proteins
4.
Rev. cuba. med ; 60(3): e1679, 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1347521

ABSTRACT

La desproporcional y alta frecuencia de órdenes médicas de anticuerpos frente al citoplasma del neutrófilo (ANCA, por sus siglas en inglés) dirigidas a nuestros laboratorios clínicos evidencia el sobreuso de la prueba de ANCA. El uso indiscriminado de esta aumenta los gastos sin beneficio de salud. El laboratorio clínico es el eslabón de la cadena diagnóstica que más siente el uso excesivo de las solicitudes de ANCA, básicamente porque genera resultados falsos positivos que comprometen la utilidad clínica de la prueba, además de recargar innecesariamente el trabajo diario del laboratorio. La prueba de ANCA es una herramienta diagnóstica muy útil para las vasculitis sistémicas primarias, pero su valor en situaciones no vasculíticas así como en otras condiciones inflamatorias y en enfermedades infecciosas o tumorales, no ha sido demostrado.1,2 El descubrimiento de los ANCA cambió...(AU)


Subject(s)
Humans , Antibodies, Antineutrophil Cytoplasmic , Clinical Laboratory Techniques/methods , Systemic Vasculitis , Fluorescence
5.
Einstein (Säo Paulo) ; 19: eRC5638, 2021. graf
Article in English | LILACS | ID: biblio-1249744

ABSTRACT

ABSTRACT Peri-implant diseases, caused by bacteria from biofilm related to dental implants, are one of the main causes of late loss of implants. In this sense, peri-implant diseases are divided into peri-implant mucositis, when it affects only the soft tissues, and peri-implantitis, when there is a bone involvement, which can lead to the failure of dental implant therapy. Thus, biofilm removal is essential for peri-implant health, allowing long-term success in implant therapy. To improve the visualization of oral biofilm, which is usually transparent or colorless, disclosing agents have been routinely used. However, disclosing agents have allergenic potential and can cause staining extrinsically in restorative and prosthetic materials, leading to aesthetic impairment. Thus, the use of fluorescence has been studied as an alternative for visualization of oral biofilm. Therefore, this report describes the use of wide-field optical fluorescence for visualization of oral biofilm associated with implants and teeth, in a routine appointment and follow-up of a partially edentulous patient with peri-implant mucositis. In addition, this report showed wide-field optical fluorescence can be used in a clinical routine of care of patients with dental implants. In this sense, wide-field optical fluorescence allowed easy and immediate visualization of the mature oral biofilm for its adequate removal, evaluation of the quality of restoration to sealing of screw access-hole of implant and identification of cariogenic lesions, without risk of allergic reactions or staining of prostheses and restorations.


RESUMO Doenças peri-implantares, causadas por bactérias de biofilme relacionadas a implantes dentários, são uma das principais causas de perda tardia de implantes. Nesse sentido, as doenças peri-implantares são divididas em mucosite peri-implantar, quando afeta apenas tecidos moles, e peri-implantite, quando há comprometimento ósseo, o que pode levar ao fracasso da terapia com implantes dentários. Assim, a remoção do biofilme é essencial para a saúde peri-implantar, permitindo sucesso a longo prazo na terapia com implantes. A fim de melhorar a visualização do biofilme oral, que geralmente é transparente ou incolor, agentes reveladores têm sido rotineiramente utilizados. No entanto, esses agentes têm potencial alergênico e podem causar manchas extrinsecamente em materiais restauradores e protéticos, levando a prejuízo estético. Assim, o uso da fluorescência tem sido estudado como alternativa para visualização do biofilme oral. Este relato descreve o uso da fluorescência óptica de campo amplo para visualização do biofilme oral associado a implantes e dentes em uma consulta de acompanhamento de rotina de uma paciente parcialmente edêntula com mucosite peri-implantar. Além disso, este relato evidenciou que a fluorescência óptica de campo amplo pode ser utilizada dentro da rotina clínica de atendimento de pacientes com implantes dentários. Nesse sentido, a fluorescência óptica de campo amplo permitiu a visualização fácil e imediata do biofilme oral maduro para sua remoção adequada, a avaliação da qualidade da restauração do selamento do orifício de acesso do parafuso do implante e a identificação de lesões cariogênicas, sem risco de reações alérgicas ou manchamento de próteses e restaurações.


Subject(s)
Humans , Dental Implants/adverse effects , Mucositis , Peri-Implantitis/etiology , Peri-Implantitis/diagnostic imaging , Biofilms , Fluorescence
6.
Chinese Journal of Biotechnology ; (12): 4095-4101, 2021.
Article in Chinese | WPRIM | ID: wpr-921490

ABSTRACT

Human induced pluripotent stem cells (hiPSCs) have the potential to differentiate into multiple cell types. Motor neurons (MNs) differentiated from hiPSCs are important models of many motor neuron diseases. To simplify the identification of MNs, lentivirus vectors were used to transfer MNs-specific promoter HB9 and red fluorescent protein (RFP) gene into hiPSCs-derived human neural stem cells (hNSCs). Stable positive cells hNSCs-HB9-RFP-Puro were obtained after antibiotic selection. Subsequently, the positive cell line was infected with lentiviruses LV-Ngn2-Sox11-GFP and LV-Isl1-Lhx3-Hygro, which overexpressed the MNs differentiation transcription factor, and differentiated to MNs directly. Differentiated mature MNs showed neuron-like structure, expressed RFP and neuron-related markers β-tubulin and choline acetyltransferase (ChAT) under the control of the MNs-specific promoter HB9. The fluorescence reporter system provides a visual method for directed differentiation and identification of MNs, and may promote the applications of MNs in disease models and drug screening.


Subject(s)
Cell Differentiation , Fluorescence , Humans , Induced Pluripotent Stem Cells , Motor Neurons , Transcription Factors
7.
Gac. méd. boliv ; 43(2): 120-126, dic. 2020. ilus
Article in Spanish | LILACS | ID: biblio-1249991

ABSTRACT

En diferentes regiones de Latinoamérica la infección por T. cruzi y Leishmania se superponen, por lo cual se reportan infecciones mixtas circulantes, debido a esto; deben realizarse pruebas diagnósticas específicas para evitar reacciones cruzadas entre estas dos patologías. OBJETIVO: determinar patrones de fluorescencia que permitan la diferenciación entre Leishmaniasis, enfermedad de Chagas e infección mixta empleando epimastigotes de T. cruzi. MÉTODOS: se empleó la técnica de Inmunofluorescencia Indirecta utilizando epimastigotes de T. cruzi (TcV autóctono) como antígeno figurado frente a un panel de muestras de suero codificados como A, B, C y D correspondientes a pacientes con infección por: Leishmaniasis (A), Infección mixta por Leishmania y Chagas(B), Enfermedad de Chagas (C) y sin ninguna de las dos infecciones (D). RESULTADOS: en los cuatro paneles de muestras se observaron diferentes patrones de intensidad de fluorescencia a nivel de membrana y núcleo de los epimastigotes de T. cruzi (TcV autóctono). CONCLUSIONES: la técnica de Inmunofluorescencia (IFI) con antígenos de epimastigotes de T. cruzi a demostrado utilidad en la diferenciación entre enfermedad de Chagas, Leishmaniasis y/o infecciones mixtas por ambos parásitos en aquellas zonas donde la coexistencia de ambas es habitual


In different regions of Latin America, infection by T. cruzi and Leishmania overlap, for which mixed circulating infections are reported, due to this; Specific diagnostic tests must be performed to avoid cross reactions between these two pathologies. OBJECTIVE: to determine fluorescence patterns that allow the differentiation between Leishmaniasis, Chagas disease and mixed infection using T. cruzi epimastigotes. METHODS: the Indirect Immunofluorescence technique was used using epimastigotes of T. cruzi (autochthonous TcV) as figurative antigen against a panel of serum samples coded as A, B, C and D corresponding to patients with infection by: Leishmaniasis (A) , Mixed infection by Leishmania and Chagas (B), Chagas disease (C) and without either of the two infections (D). RESULTS: in the four sample panels, different patterns of fluorescence intensity were observed at the membrane and nucleus level of the epimastigotes of T. cruzi (autochthonous TcV). CCONCLUSIONS: the Immunofluorescence technique (IFI) with T. cruzi epimastigote antigens has proven useful in differentiating between Chagas disease, Leishmaniasis and / or mixed infections by both parasites in areas where the coexistence of both is common.


Subject(s)
Humans , Trypanosoma cruzi , Leishmaniasis , Fluorescence , Parasites , Chagas Disease , Infections
8.
Int. j. morphol ; 38(5): 1485-1495, oct. 2020. graf
Article in English | LILACS | ID: biblio-1134466

ABSTRACT

SUMMARY: Axolotl limb regeneration is a fascinating characteristic that has attracted attention for several decades. Our previous studies on axolotl limb regeneration indicated that the satellite cells in the remnant muscles move distally into the blastema to regenerate new muscles that are separated by a gap from remnant muscles. Thereafter, the regenerative muscle fibers start to reconnect with remnant ones. In this study, the reconnection at the individual muscle fiber level was elucidated to test the hypothesis that this reconnection happens synchronously among involved muscles. Three pairs of EGFP+ mid-bud stage blastemas were transplanted onto freshly amputated stumps of RFP+ axolotls at the same thigh position to generate double fluorescence chimeric regenerative hindlimbs. These regenerative limbs were harvested very late far beyond they had reached the late differentiation stage. Fluorescence imaging of these limbs in cross sections revealed that in the proximal remnant part of the muscle fiber, reconnection occurred at a different pace among the muscles. In the major thigh muscle gracilis, the reconnection started from the periphery before it was completed. Furthermore, RFP+ muscle fibers contributed to muscle regeneration in the distal regenerative parts. Intriguingly, this red cell contribution was limited to ventral superficial muscles of the calf. This kind of double fluorescence chimeric limb regeneration model may help increase the understanding of the patterning of axolotl limb regeneration in late stages.


RESUMEN: La regeneración del miembro de Axolotl es una característica fascinante que ha llamado la atención durante varias décadas. Nuestros estudios previos sobre la regeneración del miembro del Axolotl indicaron que las células satélite en los músculos remanentes se mueven distalmente hacia el blastema para regenerar nuevos músculos que están separados por una brecha de músculos remanentes. A partir de entonces, las fibras musculares regenerativas comienzan a reconectarse con las restantes. En este estudio, se aclaró la reconexión a nivel de fibra muscular individual para probar la hipótesis de que esta reconexión ocurre sincrónicamente entre los músculos involucrados. Se trasplantaron tres pares de blastemas EGFP+ en la etapa de yema media en tocones recién amputados de axolotls RFP+ en la misma posición del muslo para generar miembros posteriores regenerativos quiméricos de fluorescencia doble. Estos miembros regenerativos se cosecharon muy tarde mucho más allá de haber alcanzado la etapa de diferenciación tardía. Las imágenes de fluorescencia de estos miembros en secciones transversales revelaron que en la parte remanente proximal de la fibra muscular, la reconexión se produjo a un ritmo diferente entre los músculos. En el músculo grácil, la reconexión comenzó desde la periferia antes de completarse. Además, las fibras musculares RFP+ contribuyeron a la regeneración muscular en las partes regenerativas distales. Curiosamente, esta contribución de glóbulos rojos se limitó a los músculos superficiales ventrales de la pantorrilla. Este tipo de modelo de regeneración quimérica de doble fluorescencia del miembro puede ayudar a aumentar la comprensión del patrón de la regeneración del miembro del Axolotl en etapas tardías.


Subject(s)
Animals , Regeneration/physiology , Extremities/physiology , Ambystoma mexicanum/physiology , Animals, Genetically Modified , Cell Transplantation , Fluorescence
9.
Arq. bras. neurocir ; 39(3): 207-212, 15/09/2020.
Article in English | LILACS | ID: biblio-1362422

ABSTRACT

Metastasis to the calvarium with direct pericranium or dural infiltration may be treated with radical surgical removal in selected cases. We describe microsurgical resection of calvarial metastases with fluorescence-guided technique using 5-aminolevulinic acid (5-ALA) in two female patients with breast cancer. Fluorescence findings were positive in both cases. Margins in the scalp and dural layer were 5-ALA negative at the end of surgical removal. Intraoperative pathology was performed in all cases to confirm if oncological limits were free of disease. One case was 5-ALA positive in the outer layer of the dura-mater and another in the pericranium. At the end of the removal in both cases, the surgicalmargins were 5-ALA fluorescence-free. Intraoperative pathology confirmed oncological limits of the resection. 5-aminolevulinic acid fluorescence-guided surgery for calvarial metastases with pericranium and/or dural extension seems to be a safe and reliable method to aid the surgical margins for complete removal, possibly delaying or avoiding adjuvant irradiation for progression control.


Subject(s)
Skull Base Neoplasms/surgery , Fluorescence , Aminolevulinic Acid , Neoplasm Metastasis , Skull/abnormalities , Skull/surgery , Retrospective Studies , Skull Base Neoplasms/diagnosis , Margins of Excision
10.
Rev. Inst. Adolfo Lutz ; 79: 1-5, 31 mar. 2020. ilus, tab
Article in Portuguese | LILACS, ColecionaSUS, SES-SP, CONASS, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO | ID: biblio-1342948

ABSTRACT

A incerteza de medição representa o nível de confiança no resultado. Para a estimativa da incerteza de medição foi empregado o cálculo do desvio padrão da reprodutibilidade intralaboratorial de 48 ensaios de contagem de bactérias heterotróficas pela técnica da membrana filtrante com detecção por fluorescência pelo uso de substrato fluorogênico em amostras de água purificada contaminadas artificialmente entre 10 e 100 UFC/mL. O valor obtido, 1,3 x 10-3 (log10), indica que a técnica utilizada pode ser uma alternativa para a estimativa da incerteza de medição em ensaios microbiológicos quantitativos de contagem de bactérias heterotróficas em amostras de água purificada. (AU)


Measurement uncertainty represents the confidence level in the result. To estimate the expanded measurement uncertainty, the standard deviation of intra-laboratory reproducibility of 48 heterotrophic bacterial count assays by fluorescence detection by the use of fluorogenic substrate on artificially contaminated purified water samples between 10 and 100 CFU/mL was used. The value obtained, 1.3 x 10-3 (log10), indicates that the technique used can be an alternative to estimate measurement uncertainty in quantitative microbiological heterotrophic bacterial count assays in purified water samples using fluorogenic substrate. (AU)


Subject(s)
Colony Count, Microbial , Water Purification , Uncertainty , Heterotrophic Bacteria , Fluorescence
11.
Einstein (Säo Paulo) ; 18: eAO4954, 2020. graf
Article in English | LILACS | ID: biblio-1056032

ABSTRACT

ABSTRACT Objective: To evaluate the magnetic hyperthermia therapy in glioblastoma tumor-on-a-Chip model using a microfluidics device. Methods: The magnetic nanoparticles coated with aminosilane were used for the therapy of magnetic hyperthermia, being evaluated the specific absorption rate of the magnetic nanoparticles at 300 Gauss and 305kHz. A preculture of C6 cells was performed before the 3D cells culture on the chip. The process of magnetic hyperthermia on the Chip was performed after administration of 20μL of magnetic nanoparticles (10mgFe/mL) using the parameters that generated the specific absorption rate value. The efficacy of magnetic hyperthermia therapy was evaluated by using the cell viability test through the following fluorescence staining: calcein acetoxymethyl ester (492/513nm), for live cells, and ethidium homodimer-1 (526/619nm) for dead cells dyes. Results: Magnetic nanoparticles when submitted to the alternating magnetic field (300 Gauss and 305kHz) produced a mean value of the specific absorption rate of 115.4±6.0W/g. The 3D culture of C6 cells evaluated by light field microscopy imaging showed the proliferation and morphology of the cells prior to the application of magnetic hyperthermia therapy. Fluorescence images showed decreased viability of cultured cells in organ-on-a-Chip by 20% and 100% after 10 and 30 minutes of the magnetic hyperthermia therapy application respectively. Conclusion: The study showed that the therapeutic process of magnetic hyperthermia in the glioblastoma on-a-chip model was effective to produce the total cell lise after 30 minutes of therapy.


RESUMO Objetivo: Avaliar a terapia de magneto-hipertermia em modelo de tumor de glioblastoma on-a-Chip. Métodos: As nanopartículas magnéticas recobertas com aminosilana foram utilizadas para a terapia da magneto-hipertermia, sendo avaliada a taxa de absorção específica das nanopartículas magnéticas em 300 Gauss e 305kHz. Uma pré-cultura de células C6 foi realizada e, seguidamente, foi feito o cultivo das células 3D no chip. O processo de magneto-hipertermia no chip foi realizado após administração de 20μL de nanopartículas magnéticas (10mgFe/mL), utilizando os parâmetros que geraram o valor da taxa de absorção específica. A eficácia da terapia de magneto-hipertermia foi avaliada pela viabilidade celular por meio dos corantes fluorescentes acetoximetiléster de calceína (492/513nm), para células vivas, e etídio homodímero-1 (526/619nm), para células mortas. Resultados: As nanopartículas magnéticas, quando submetidas ao campo magnético alternado (300 Gauss e 305kHz), produziram um valor médio da taxa de absorção específica de 115,4±6,0W/g. A cultura 3D das células C6 avaliada por imagem de microscopia de campo claro mostrou a proliferação e a morfologia das células antes da aplicação da terapia de magneto-hipertermia. As imagens de fluorescência mostraram diminuição da viabilidade das células cultivadas no organ-on-a-Chip em 20% e 100% após 10 e 30 minutos, respectivamente, da aplicação da terapia de magneto-hipertermia. Conclusão: O processo terapêutico da magneto-hipertermia no modelo de tumor glioblastoma on-a-chip foi eficaz para produzir lise total das células após 30 minutos de terapia.


Subject(s)
Animals , Rats , Glioblastoma/therapy , Cell Culture Techniques/methods , Lab-On-A-Chip Devices , Magnetite Nanoparticles/therapeutic use , Hyperthermia, Induced/methods , Temperature , Time Factors , Cell Survival , Reproducibility of Results , Treatment Outcome , Cell Line, Tumor , Magnetic Fields , Fluorescence
12.
Araçatuba; s.n; 2020. 43 p. graf, ilus.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1396449

ABSTRACT

A remoção de facetas de resina composta quando o retratamento se faz necessário representa um desafio ao clínico no sentido de preservação do remanescente dental. Sendo assim, o objetivo deste estudo foi avaliar dispositivos auxiliares na precisão dos retratamentos de facetas diretas, por meio de escaneamento digital. Setenta e cinco espécimes de dente bovino (10 x 8 mm ­ cor A1) foram selecionados, e então foi realizado o escaneamento inicial (T0). Em seguida, os espécimes foram preparados para faceta de resina composta e novamente escaneados (T1) para verificação da padronização dos preparos. As superfícies dentárias foram então restauradas com sistema adesivo e resina composta. Os espécimes foram divididos em 5 grupos (n=15): remoção sem dispositivo auxiliar (CN ­ grupo controle), remoção com alta rotação com LED branco (AL), remoção com alta rotação com luz ultra-violeta (UV), remoção com motor elétrico e multiplicadora 1/5 (MT) e remoção com uso de lupa de aumento 2,5x (MN). A remoção das facetas e o re-preparo dos espécimes foi feito por outro operador, sendo então realizado um novo escaneamento (T2). A alteração volumétrica entre T2 e T1, assim como, as áreas de desgaste dentário e/ou presença de resíduos de resina composta foram avaliados. A média entre desgaste e presença de resíduos entre T2 e T1 foi também realizada. Os dados foram submetidos aos testes estatísticos de Kruskal-Wallis e pós teste de Dunn ao nível de significância de 5%. Não foi encontrada diferença estatística entre os grupos entre T1 e T0, com uma média de desgaste de 0,28 mm (± 0,07), p ≥ 0.05. Somente houve diferença entre os grupos para as análises referentes às áreas de desgaste e de presença de resíduos (p ≤ 0,05). Ocorreu maiores áreas de desgaste para o grupo MN, sendo estatisticamente superior aos grupos CN e MT (p≤ 0,05). O oposto ocorreu para áreas de resíduos, sendo que o grupo MN apresentou as menores áreas de resíduos, sendo estatisticamente inferior aos grupos CN e MT (p≤ 0,05). Considerando a média entre desgaste e resíduos, ocorreu um desgaste adicional após o re-preparo, indepedente do grupo (p≥ 0,05). Apesar do uso de lupa odontológica ser mais efetivo para remoção da resina composta durante o retratamento de faceta direta, este proporciona maior desgaste da estrutura dentária. A similaridade da fluorescência entre a resina composta e o substrato dentário minimizou os benefícios do dispositivo com luz UV(AU)


The removal of direct composite veneers when the retreatment is necessary represents a challenge to the clinician in the sense of dental preservation. Thus, the aim of this study was to evaluate auxiliary devices, in the precision of direct composite veneers retreatments, through digital scanning. Seventy-five bovine tooth specimens (10 x 8 mm - A1 shade) were selected and the initial scanning (T0) was performed. Then, the specimens were prepared for direct composite veneer and rescanned (T1) to verify the standardization of the preparations. The dental surfaces were then restored with adhesive system and composite resin. The specimens were divided into 5 groups (n=15): removal without auxiliary device (CN - control group), removal with a hand piece with a white LED (WL), removal with a hand piece with an UV light source (UV), removal with electric motor and multiplier 1/5 hand piece (MT); and removal with the use of a 2.5x dental loupe (MN). The removal of the restorations and the re-preparation of the specimens were done by another operator, and a new scanning (T2) was performed. The volumetric change between T2 and T1, as well as the areas of additional dental wear and presence of composite resin residues were evaluated. The mean considering additional wear and the presence of residues between T2 and T1 were also performed. The data were submitted to Kruskal-Wallis and Dunn's as post-test, at significance level of 5%. No statistical difference among the groups were found between T1 and T0 with a wear mean of 0.28 mm (±0.07), p≥ 0.05. There were only statistical differences between the groups for the analyses related to the areas of wear and presence of residues (p≤ 0.05). There were greater areas of wear for MN group, being statistically superior to CN and MT groups (p≤ 0.05). The opposite occurred for areas of presence of residues, with the MN group presenting smaller areas of residues, being statistically lower than the CN and MT groups (p≤ 0.05). Considering the average between wear and residues, additional wear occurred after re-preparation, regardless of the group (p≥ 0.05). Although the use of dental loupe is more effective for removal of composite resin during the retreatment of direct composite veneers, it provides greater wear of the dental structure. The fluorescence similarity between the composite resin and the dental substrate minimized the benefits of UV light device(AU)


Subject(s)
Dental High-Speed Equipment , Retreatment , Dental Instruments , Dental Veneers , Resins, Synthetic , Ultraviolet Rays , Color , Composite Resins , Dental Cements , Dental Enamel , Dentin , Tooth Wear , Fluorescence
15.
Article in Korean | WPRIM | ID: wpr-811330

ABSTRACT

PURPOSE: The Ocular Surface Disease Index (OSDI) and the Standardized Patient Evaluation of Eye Dryness (SPEED) which are standard questionnaires of dry eye syndrome were used to determine the associations between clinical dry eye tests and meibomian gland dysfunctions (MGD).METHODS: Forty-one patients with MGD were enrolled in this study. The score of the dry eye syndrome questionnaire and the degree of blepharitis (score: 0–4), Schirmer test results, degree of fluorescence staining of cornea (Oxford Grading System), tear break-up time (TBUT), Pentacam imaging, and anterior segment optical coherence tomography results were used to compare and analyze the results of each test for possible correlations with the dry eye questionnaire answers.RESULTS: There was a significant correlation between OSDI and SPEED (R = 0.278, p = 0.011). SPEED was correlated with the Oxford grade (R = 0.478, p < 0.001) and MGD grade (R = 0.280, p = 0.011) while there was no significant correlation with corneal aberrations, tear meniscus height, tear meniscus area, Schirmer test results, or TBUT. The OSDI correlated with the MGD grade (R = 0.651, p < 0.001), TBUT (R = −0.360, p = 0.001), and age (R = −0.230, p = 0.037). Using multiple regression analyses, the MGD grade affected the OSDI (β = 0.580, p < 0.001) and the Oxford grade significantly influenced the SPEED (β = 0.447, p < 0.001).CONCLUSIONS: In Koreans, the OSDI questionnaire answers were associated with the MGD grade and SPEED questionnaire answers were associated with the corneal surface status. The OSDI questionnaire was therefore clinically useful in patients with meibomian gland dysfunction.


Subject(s)
Blepharitis , Cornea , Dry Eye Syndromes , Fluorescence , Humans , Meibomian Glands , Tears , Tomography, Optical Coherence
16.
Article in English | WPRIM | ID: wpr-811193

ABSTRACT

Breast adenomyoepitheliomas are composed of a biphasic proliferation of myoepithelial cells around small epithelial-lined spaces. Due to the rarity of adenomyoepitheliomas, the molecular data describing them are limited. Adenomyoepitheliomas are considered to be benign or have low malignant potential, and be prone to local recurrence. Malignant transformation has been associated with homozygous deletion of CDKN2A or somatic mutations in TERT, but remains unexplained in many cases. Here, we describe a case of carcinomatous transformation of both epithelial and myoepithelial cells in an estrogen receptor-negative adenomyoepithelioma caused by amplification of MYC. Break-apart fluorescence in situ hybridization revealed an increase in the MYC gene copy number (3–4 copies/cell in 37%, > 4 copies/cell in 40%). Deregulation of MYC is responsible for uncontrolled proliferation and cellular immortalization in basal-like breast cancers. Our case demonstrates that genomic instability events associated with gene amplification may be involved in the carcinogenesis of malignant adenomyoepitheliomas.


Subject(s)
Adenomyoepithelioma , Breast Neoplasms , Breast , Carcinogenesis , Estrogens , Fluorescence , Gene Amplification , Genes, myc , Genomic Instability , In Situ Hybridization , In Situ Hybridization, Fluorescence , Recurrence
18.
Chonnam Medical Journal ; : 20-26, 2020.
Article in English | WPRIM | ID: wpr-787278

ABSTRACT

We examined the effect of fluoxetine, a selective serotonin reuptake inhibitor antidepressant, on neuronal viability in mouse cortical near-pure neuronal cultures. Addition of fluoxetine to the media for 24 hours induced neuronal death in a concentration-dependent manner. To delineate the mechanisms of fluoxetine-induced neuronal death, we investigated the effects of trolox, cycloheximide (CHX), BDNF, z-VAD-FMK, and various metal-chelators on fluoxetine-induced neuronal death. Neuronal death was assessed by MTT assay. The addition of 20 µM fluoxetine to the media for 24 hours induced 60–70% neuronal death, which was associated with the hallmarks of apoptosis, chromatin condensation and DNA laddering. Fluoxetine-induced death was significantly attenuated by CHX, BDNF, or z-VAD-FMK. Treatment with antioxidants, trolox and ascorbate, also markedly attenuated fluoxetine-induced death. Interestingly, some divalent cation chelators (EGTA, Ca-EDTA, and Zn-EDTA) also markedly attenuated the neurotoxicity. Fluoxetine-induced reactive oxygen species (ROS) generation was measured using the fluorescent dye 2′,7′-dichlorofluorescin diacetate. Trolox and bathocuproine disulfonic acid (BCPS), a cell membrane impermeable copper ion chelator, markedly attenuated the ROS production and neuronal death. However, deferoxamine, an iron chelator, did not affect ROS generation or neurotoxicity. We examined the changes in intracellular copper concentration using a copper-selective fluorescent dye, Phen Green FL, which is quenched by free copper ions. Fluoxetine quenched the fluorescence in neuronal cells, and the quenching effect of fluoxetine was reversed by co-treatment with BCPS, however, not by deferoxamine. These findings demonstrate that fluoxetine could induce apoptotic and oxidative neuronal death associated with an influx of copper ions.


Subject(s)
Animals , Antioxidants , Apoptosis , Brain-Derived Neurotrophic Factor , Cell Death , Cell Membrane , Chelating Agents , Chromatin , Copper , Cycloheximide , Deferoxamine , DNA , Fluorescence , Fluoxetine , Ions , Iron , Mice , Neurons , Reactive Oxygen Species , Serotonin
19.
Chinese Journal of Biotechnology ; (12): 1051-1059, 2020.
Article in Chinese | WPRIM | ID: wpr-826871

ABSTRACT

Neurotransmitters play an important role in nervous system. Temporal and spatial changes of neurotransmitter distribution are crucial to information processing in neural networks. Biosensors that can visually monitor neurotransmitters are one of the vital tools to explore a variety of physiological and pathological activities. This article reviews recent advances in monitoring neurotransmitters with high temporal and spatial resolution, and introduces the latest fluorescent imaging methods for typical neurotransmitters, including glutamate, dopamine, γ-aminobutyric acid and acetylcholine. The article also summarizes the basic principles, advantages and disadvantages of various visually detection methods, and provides systematic suggestions for designing neurotransmitter sensors with high temporal and spatial resolution.


Subject(s)
Animals , Biosensing Techniques , Fluorescence , Humans , Neurotransmitter Agents , Metabolism
20.
Chinese Journal of Biotechnology ; (12): 1216-1222, 2020.
Article in Chinese | WPRIM | ID: wpr-826856

ABSTRACT

A rapid and simple method to detect tumor markers in liver cancer was established by combining immunochromatography technique with fluorescent microsphere labeling. According to the principle of double antibody sandwich, the cytoskeleton-associated protein 4 (CKAP4) paired antibody was used as the labeled and coated antibody, and the goat anti-rabbit polyclonal antibody was used as the quality control line coated antibody in the preparation of the CKAP4 fluorescent immunochromatographic test strips. After the preparation, the test strips were evaluated on various performance indicators, such as linearity, precision and stability. The CKAP4 immunochromatographic strip prepared by time-resolved fluorescent microspheres had high sensitivity, and good specificity. Its precision was within 15%, recovery between 85% and 115%, and linear range between 25 and 1 000 pg/mL. The test strip could be kept stable at 37 °C for 20 days, and it correlated well with commercial ELISA kits. The CKAP4 fluorescence immunochromatography method can quantitatively detect the content of CKAP4 in serum. Furthermore, it is rapid, sensitive, simple, economical and single-person operation. This method has the potential of becoming a new method for the diagnosis and treatment of liver cancer.


Subject(s)
Animals , Antibodies , Metabolism , Chromatography, Affinity , Fluorescence , Humans , Liver Neoplasms , Diagnosis , Membrane Proteins , Molecular Diagnostic Techniques , Methods , Sensitivity and Specificity
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