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1.
Braz. j. biol ; 83: e250179, 2023. graf
Article in English | LILACS, VETINDEX | ID: biblio-1339372

ABSTRACT

Abstract Diabetes mellitus (DM) is a non-communicable disease throughout the world in which there is persistently high blood glucose level from the normal range. The diabetes and insulin resistance are mainly responsible for the morbidities and mortalities of humans in the world. This disease is mainly regulated by various enzymes and hormones among which Glycogen synthase kinase-3 (GSK-3) is a principle enzyme and insulin is the key hormone regulating it. The GSK-3, that is the key enzyme is normally showing its actions by various mechanisms that include its phosphorylation, formation of protein complexes, and other cellular distribution and thus it control and directly affects cellular morphology, its growth, mobility and apoptosis of the cell. Disturbances in the action of GSK-3 enzyme may leads to various disease conditions that include insulin resistance leading to diabetes, neurological disease like Alzheimer's disease and cancer. Fluoroquinolones are the most common class of drugs that shows dysglycemic effects via interacting with GSK-3 enzyme. Therefore, it is the need of the day to properly understand functions and mechanisms of GSK-3, especially its role in glucose homeostasis via effects on glycogen synthase.


Resumo O diabetes mellitus (DM) é uma doença não transmissível em todo o mundo, na qual existe nível glicêmico persistentemente alto em relação à normalidade. O diabetes e a resistência à insulina são os principais responsáveis ​​pelas morbidades e mortalidades de humanos no mundo. Essa doença é regulada principalmente por várias enzimas e hormônios, entre os quais a glicogênio sintase quinase-3 (GSK-3) é uma enzima principal e a insulina é o principal hormônio que a regula. A GSK-3, que é a enzima-chave, normalmente mostra suas ações por vários mecanismos que incluem sua fosforilação, formação de complexos de proteínas e outras distribuições celulares e, portanto, controla e afeta diretamente a morfologia celular, seu crescimento, mobilidade e apoptose do célula. Perturbações na ação da enzima GSK-3 podem levar a várias condições de doença que incluem resistência à insulina que leva ao diabetes, doenças neurológicas como a doença de Alzheimer e câncer. As fluoroquinolonas são a classe mais comum de drogas que apresentam efeitos disglicêmicos por meio da interação com a enzima GSK-3. Portanto, é necessário hoje em dia compreender adequadamente as funções e mecanismos da GSK-3, principalmente seu papel na homeostase da glicose via efeitos na glicogênio sintase.


Subject(s)
Humans , Insulin Resistance , Diabetes Mellitus , Glycogen Synthase Kinase 3 , Glucose , Homeostasis
2.
Rev. Méd. Inst. Mex. Seguro Soc ; 60(2): 211-223, abr. 2022. ilus, tab
Article in Spanish | LILACS | ID: biblio-1367402

ABSTRACT

El retículo endoplásmico es un organelo abundante, dinámico y sensor de energía. Sus abundantes membranas, rugosa y lisa, se encuentran distribuidas en diferentes proporciones dependiendo del linaje y requerimiento celular. Su función es llevar a cabo la síntesis de proteínas y lípidos, y es el almacén principal de Ca2+ intracelular. La sobrecarga calórica y la glucolipotoxicidad generada por dietas hipercalóricas provoca la alteración del retículo endoplásmico, activando la respuesta a proteínas mal plegadas (UPR, Unfolded Protein Response, por sus siglas en inglés) como reacción al estrés celular relacionado con el retículo endoplásmico y cuyo objetivo es restablecer la homeostasis del organelo al disminuir el estrés oxidante, la síntesis de proteínas y la fuga de Ca2+. Sin embargo, durante un estrés crónico, la UPR induce formación de especies reactivas de oxígeno, inflamación y apoptosis, exacerbando el estado del retículo endoplásmico y propagando un efecto nocivo para los demás organelos. Es por ello que el estrés del retículo endoplásmico se ha considerado un inductor del inicio y desarrollo de enfermedades metabólicas, incluido el agravamiento de COVID-19. Hasta el momento, existen pocas estrategias para reestablecer la homeostasis del retículo endoplásmico, las cuales son dirigidas a los sensores que desencadenan la UPR. Por tanto, se justifica con urgencia la identificación de nuevos mecanismos y terapias novedosas relacionadas con mitigar el impacto del estrés del retículo endoplásmico y las complicaciones asociadas.


The endoplasmic reticulum is an abundant, dynamic and energy-sensing organelle. Its abundant membranes, rough and smooth, are distributed in different proportions depending on the cell lineage and requirement. Its function is to carry out protein and lipid synthesis, and it is the main intracellular Ca2+ store. Caloric overload and glycolipotoxicity generated by hypercaloric diets cause alteration of the endoplasmic reticulum, activating the Unfolded Protein Response (UPR) as a reaction to cellular stress related to the endoplasmic reticulum and whose objective is to restore the homeostasis of the organelle by decreasing oxidative stress, protein synthesis and Ca2+ leakage. However, during chronic stress, the UPR induces reactive oxygen species formation, inflammation and apoptosis, exacerbating the state of the endoplasmic reticulum and propagating a deleterious effect on the other organelles. This is why endoplasmic reticulum stress has been considered an inducer of the onset and development of metabolic diseases, including the aggravation of COVID-19. So far, few strategies exist to reestablish endoplasmic reticulum homeostasis, which are targeted to sensors that trigger UPR. Therefore, the identif ication of new mechanisms and novel therapies related to mitigating the impact of endoplasmic reticulum stress and associated complications is urgently warranted.


Subject(s)
Humans , Dietary Carbohydrates/adverse effects , Dietary Fats/adverse effects , Endoplasmic Reticulum/metabolism , Endoplasmic Reticulum Stress/physiology , COVID-19/complications , Metabolic Diseases/etiology , COVID-19/therapy , Homeostasis
3.
Lima; Perú. Ministerio de Salud. Centro Nacional de Epidemiología, Prevención y Control de Enfermedades; 1 ed; Mar. 2022. 308-39 p. ilus.(Boletín Epidemiológico, 31, SE 10).
Monography in Spanish | LILACS, LIPECS, MINSAPERU | ID: biblio-1373028

ABSTRACT

El riñón es un órgano clave para mantener el balance "homeostasis" del medio interno (agua, electrolitos, equilibrio ácido básico y el equilibrio osmótico), depura la sangre de productos de desechos metabólicos endógenos y exógenos, regula el volumen circulante efectivo y la presión arterial, y sintetiza la eritropoyetina y la vitamina D. Todas estas funciones se ejecutan en el nefrón que constituye la unidad funcional renal


Subject(s)
Epidemiologic Studies , Epidemiologic Measurements , Epidemiological Monitoring , Arterial Pressure , Homeostasis , Kidney
4.
Rev. bras. promoç. saúde (Impr.) ; 35(1): 1-14, 20220125.
Article in English, Portuguese | LILACS | ID: biblio-1377780

ABSTRACT

Objetivo: Avaliar os benefícios da técnica Homeostase Quântica Informacional (HQI) sobre a saúde física e emocional de pessoas adultas. Métodos: Estudo experimentalrandomizado quali-quantitativo com grupo controle, com pré-teste e pós-teste, realizado na cidade de Campinas, São Paulo, Brasil. Obtiveram-se os dados entre julho e setembro de 2018 com 50 adultos na faixa etária de 45 a 60 anos, e os instrumentos utilizados avaliaram variáveis de natureza sociodemográfica, de saúde física e emocional. Para análise dos dados utilizou-se o teste qui-quadrado ou o teste exato de Fisher. Para comparação das variáveis numéricas entre os dois grupos utilizou-se o teste de Mann-Whitney e para comparação das variáveis categóricas utilizou-se o teste de Cochran seguido do teste de McNemar. O nível de significância adotado para os testes estatísticos ficou ajustado em p<0,05 e para os dados qualitativos utilizou-se a análise de conteúdo. Resultados: Os resultados mostraram ganhos estatisticamente significantes nos indivíduos do Grupo 1, que receberam o tratamento de HQI, nas avaliações para atividade física (p=0,046), escore de ansiedade (p=0,013), de sofrimento emocional (p=0,008 e p=0,019) e de tontura (p=0,021), quando comparados com o Grupo 2, controle. Conclusão: Os dados obtidos permitem afirmar que a HQI proporciona benefícios para a saúde física e emocional de pessoas adultas. Bem como o seu emprego poderia tornar melhor o dia a dia das pessoas, diminuindo o custo de tratamentos prolongados e onerosos.


Objective: To assess the benefits of the Quantum Information Homeostasis (QIH) technique for the physical and emotional health of adults. Methods: A qualitative and quantitative randomized experimental study with a control group, pre-test and posttest was carried out in the city of Campinas, São Paulo. Data were collected between July and September 2018 from 50 adults aged 45 to 60 years, and the instruments used assessed sociodemographic and physical and emotional health variables. For data analysis, we used either the chi-square test or the Fisher's exact test. To compare the numerical variables between the two groups, the Mann-Whitney test was used, and to compare thecategoricalvariables, the Cochran test followed by the McNemar est was used. The level of significance adopted for the statistical tests was adjusted to p<0.05 and content analysis was used for qualitative data. Results: The results showed statistically significant gains in individuals in group 1, who received the QIH treatment, in the assessments for physical activity (p=0.046), anxiety score (p=0.013), emotional distress (p=0.008 and p= 0.019) and dizziness (p=0.021), when compared to group 2, controls. Conclusion: The data obtained allow us to state that QIH provides benefits for the physical and emotional health of adults. Also, its use could make people's daily lives better, reducing the cost of long and expensive treatments


Objetivo: Evaluar los beneficios de la técnica Homeostasis Quántica Informacional (HQI) sobre la salud física y emocional de personas adultas. Métodos: Estudio experimental randomizado cuali-cuantitativo con grupo control, pre y postest realizado en la ciudad de Campinas, São Paulo, Brasil. Se ha recogido los datos entre julio y septiembre de 2018 con 50 adultos en la franja de edad entre 45 y 60 años. Los instrumentos utilizados evaluaron las variables de naturaleza sociodemográfica, salud física y emocional. Se utilizó la prueba chi-cuadrado o exacto de Fisher para el análisis de los datos. Para la comparación de las variables numéricas entre los grupos se utilizó la prueba de Mann-Whitney y para la comparación de las variables categóricas se utilizó la prueba de Cochran seguida de la prueba de McNemar. El nivel de significancia adoptado para las pruebas estadísticas fue de p<0,05 y para los datos cualitativos se utilizó el análisis de contenido. Resultados: Los resultados mostraron ganancias estadísticamente significantes para los individuos del Grupo 1, que han sido tratados con la HQI, en las evaluaciones de actividad física (p=0,046), la puntuación de ansiedad (p=0,013), el sufrimiento emocional (p=0,008 e p=0,019) y de vértigo (p=0,021) comparados con el Grupo 2, el control. Conclusión: Los datos permiten afirmar que la HQI proporciona beneficios para la salud física y emocional de personas adultas, así como su utilización podría mejorar el día a día de las personas disminuyendo el coste de los tratamientos a largo plazo y costosos.


Subject(s)
Complementary Therapies , Homeostasis , Longevity , Middle Aged
5.
Braz. j. biol ; 82: e233567, 2022. tab, graf
Article in English | LILACS | ID: biblio-1249220

ABSTRACT

Abstract This research was carried out aiming at evaluating the effects of nitrate and ammonium ions on nutrient accumulation, biochemical components and yield of Italian zucchini (cv. Caserta) grown in a hydroponic system under salt stress conditions. The experiment was carried out in a greenhouse utilizing an experimental design in randomized blocks, arranged in a 2 x 5 factorial scheme, with 4 replications. The treatments consisted of two forms of nitrogen (nitrate - NO3- and ammonium - NH4+) and 5 electrical conductivity levels of irrigation water (ECw) (0.5, 2.0, 3.5, 5.0 and 6.5 dS m-1). The analysis of the results indicated that supply of N exclusively in NH4+ form promotes greater damage to the leaf membrane and reduction in accumulation of macronutrients and higher Na+/K+, Na+/Ca++ and Na+/Mg++ ratios in the shoots of zucchini plants. Electrical conductivity of irrigation water above 2.0 dS m-1 reduces the accumulation of nutrients in shoot and yield of Italian zucchini plant. The toxicity of NH4+ under Italian zucchini plants overlap the toxicity of the salinity, since its fertilization exclusively with this form of nitrogen inhibits its production, being the NO3- form the most suitable for the cultivation of the species.


Resumo Este trabalho foi desenvolvido com o objetivo de avaliar os efeitos dos íons nitrato e amônio sobre o acúmulo de nutrientes e produção da abobrinha italiana (cv. Caserta) cultivada em sistema hidropônico sob estrese salino. O experimento foi conduzido em casa de vegetação utilizando o delineamento experimental em blocos casualizados, arranjados em esquema fatorial 2 x 5, com 4 repetições. Os tratamentos foram constituídos de duas formas de nitrogênio (nitrato - NO3- e amônio - NH4+) e cinco níveis de condutividade elétrica da água de irrigação (CEa) (0,5; 2,0; 3,5; 5,0 e 6,5 dS m-1). As análises dos resultados indicaram que suprimento de N exclusivamente em forma de NH4+ promove maiores danos na membrana foliar e redução no acúmulo de macronutrientes e maiores relações Na+/K+, Na+/Ca++ e Na+/Mg++ na parte aérea das plantas de abobrinha. A irrigação com água a cima de 2,0 dS m-1 reduz o acúmulo de nutrientes na parte aérea das plantas e a produção de abobrinha. A toxicidade do NH4+ sob abobrinha italiana sobrepõe-se à toxicidade da salinidade, pois a fertilização exclusiva com esta forma de nitrogênio inibe sua produção, sendo a forma NO3- a mais adequada para o cultivo da espécie.


Subject(s)
Ammonium Compounds , Nitrogen , Plant Roots/chemistry , Salt Stress , Homeostasis , Italy , Nitrates
6.
Esc. Anna Nery Rev. Enferm ; 26: e20210368, 2022.
Article in Portuguese | LILACS, BDENF | ID: biblio-1364837

ABSTRACT

RESUMO Objetivo estimular a reflexão acerca da assistência de enfermagem ao paciente com sepse a partir dos quatro princípios de conservação propostos pelo modelo conceitual de Myra Levine. Método estudo teórico-reflexivo sobre a relação existente entre os princípios de conservação de Levine e o paciente com sepse. Resultados vigilância aos princípios da conservação da energia observando oferta do oxigênio, idade dos pacientes e os parâmetros energéticos (sinais vitais); da integridade estrutural ao reconhecer precocemente às disfunções orgânicas no pacote hora-1; da integridade pessoal ao preservar a identidade do cliente com dificuldade de verbalizar ou outra condição e da integridade social ao relacionar-se com o paciente e família incluindo-os no processo de cuidado. Conclusão e implicações para a prática os princípios de conservação do modelo proposto apresentam relação com a assistência realizada pela equipe de enfermagem na manutenção do equilíbrio físico, pessoal e social do paciente com sepse. Espera-se que os enfermeiros articulem o conhecimento científico geral da assistência ao paciente com sepse ao seu conhecimento específico, por meio de um referencial teórico, para a promoção da adaptação, conservação e integridade do indivíduo.


RESUMEN Objetivo estimular la reflexión acerca de la atención de enfermería al paciente con sepsis a partir de los cuatro principios de conservación propuestos por el modelo conceptual de Myra Levine. Método estudio teórico-reflexivo sobre la relación entre los principios de conservación de Levine y el paciente con sepsis. Resultados vigilancia de los principios de conservación de la energía, observando el aporte de oxígeno, la edad de los pacientes y los parámetros energéticos (signos vitales); de la integridad estructural mediante el reconocimiento temprano de las disfunciones orgánicas en el paquete hora-1; de la integridad personal al preservar la identidad del cliente con dificultad para verbalizar u otra condición e de la integridad social al relacionarse con el paciente y su familia, incluyéndolos en el proceso de cuidado. Conclusión e implicaciones para la práctica los principios de conservación del modelo propuesto se relacionan con la atención brindada por el equipo de enfermería en el mantenimiento del equilibrio físico, personal y social del paciente con sepsis. Se espera que los enfermeros articulen el conocimiento científico general del cuidado del paciente con sepsis con sus conocimientos específicos, a través de un referencial teórico, para promover la adaptación, conservación e integridad del individuo.


ABSTRACT Objective encourage reflection on nursing care for patients with sepsis based on the four conservation principles proposed by Myra Levine's conceptual model. Method theoretical-reflective study on the relationship between Levine's conservation principles and the patient with sepsis. Results attentiveness to the principles of energy conservation, observing oxygen supply, patients' age and energy parameters (vital signs); of structural integrity by early recognition of organic dysfunctions in the hour-1 bundle; of personal integrity when preserving the identity of the client with difficulty to verbalize or another condition and social integrity when relating to the patient and family, including them in the care process. Conclusion and implications for practice the conservation principles of the proposed model are presented in relation to assistance provided by the nursing team in maintaining the physical, personal and social balance of the patients with sepsis. Nurses are expected to articulate the general scientific knowledge of sepsis patient care with their specific knowledge, through a theoretical framework, to promote the individual's adaptation, conservation and integrity.


Subject(s)
Humans , Nursing Theory , Sepsis/nursing , Nursing Care , Homeostasis , Nursing, Team
7.
Rev. Ateneo Argent. Odontol ; 66(1): 34-46, 2022. ilus, tab
Article in Spanish | LILACS | ID: biblio-1380253

ABSTRACT

La población mayor de 60 años es el grupo etario de mayor crecimiento en el mundo. Debido a que la depresión es una patología frecuente en la persona adulta mayor y anciana, los inhibidores de la recap- tación de la serotonina (ISRS) son el tratamiento de primera línea de elección. Este trabajo referencia la asociación del consumo de estos fármacos con la disminución de la densidad ósea mineral (DMO), el riesgo de fracturas y su repercusión en la atención odontológica. Además, incluye una breve descripción de la homeostasis ósea y la relación depresión-carga alostática. El trabajo interdisciplinario y una correcta anamnesis pueden detectar posibles complicaciones y riesgos vinculados con este tipo de medicamen- tos. Ello facilitaría un mejor manejo, más aún en el adulto mayor, donde una pequeña variable puede repercutir en su integridad (AU)


The population over 60 is the fastest growing age group in the world. Depression is a frequent pathology in the elderly and the elderly, with serotonin reuptake inhibitors (SSRI) being the 1st line treatment of choice. The association of the consumption of this drug with a decrease in bone mineral density (BMD), risk of fractures and its impact on dental care are referenced in this work. In addition, it includes a brief description of bone homeostasis and the depression-allostatic load relationship. Interdisciplinary work and a correct anamnesis can detect possible complications and risks linked to this type of medication, facilitating better management and even more so in the elderly, where a small variable can affect their integrity (AU)


Subject(s)
Humans , Male , Female , Aged , Aged, 80 and over , Dental Care for Aged/methods , Serotonin Uptake Inhibitors/adverse effects , Depression/complications , Antidepressive Agents/adverse effects , Bone Density/drug effects , Dental Implants/adverse effects , Risk Factors , Age Factors , Bone Remodeling/physiology , Serotonin Uptake Inhibitors/therapeutic use , Dental Restoration Failure , Fractures, Bone/prevention & control , Allostasis , Homeostasis
8.
Odontoestomatol ; 24(39): 1-14, 2022.
Article in Spanish | LILACS, BNUY, BNUY-Odon | ID: biblio-1370336

ABSTRACT

Numerosos reportes demuestran la presencia de biomarcadores de estrés oxidativo en la saliva de fumadores y hay un creciente interés en correlacionar estos procesos moleculares con la etiología de algunas enfermedades orales, como la periodontitis, una enfermedad inmunoinflamatoria crónica relacionada con un desequilibrio de la homeostasis redox celular. Objetivo: realizar una revisión narrativa sobre la relación entre la disminución de la capacidad antioxidante salival inducida por humo de tabaco, la periodontitis y el potencial uso de farmacología redox para el tratamiento de esta patología. Métodos: se realizó una búsqueda bibliográfica en bases de datos como PUBMED (NLM, NIH, NCBI) y SciELO. Resultados: existe evidencia que relaciona la baja capacidad antioxidante salival con un retraso en el restablecimiento de las condiciones normales en la cavidad oral ante el desarrollo de periodontitis. A su vez, el estado inflamatorio asociado colabora sinérgicamente, provocando un mayor daño tisular con pérdida de tejidos de soporte dentario, fenómeno que podría ser modulado por la acción de farmacología redox. Conclusiones: la intervención con farmacología redox, podría atenuar los biomarcadores de progresión de la enfermedad periodontal, constituyendo una herramienta prometedora para utilizar en conjunto con las estrategias de tratamiento tradicionales.


Numerous reports demonstrate the presence of oxidative stress biomarkers in the saliva of smokers and there is a growing interest in correlating these molecular processes with the etiology of some oral diseases, such as periodontitis, a chronic immunoinlammatory disease related to an imbalance of cellular redox homeostasis. Aims: achieve a narrative review on the relationship between the decrease in salivary antioxidant capacity induced by tobacco smoke, periodontitis, and the potential use of redox pharmacology for the treatment of this pathology. Methods: a bibliographic search was carried out in databases such as PUBMED (NLM, NIH, NCBI) and SciELO. Results: there is evidence that relates the low salivary antioxidant capacity with a delay in the reestablishment of normal conditions in the oral cavity before the development of periodontitis. In turn, the associated inflammatory state collaborates synergistically, causing greater tissue damage with loss of dental support tissues, a phenomenon that could be modulated by the action of redox pharmacology. Conclusions: intervention with redox pharmacology could attenuate the biomarkers of periodontal disease progression, constituting a promising tool to be used in conjunction with traditional treatment strategies.


Muitos artigos demonstram a presença de biomarcadores de estresse oxidativo na saliva de fumantes e há um interesse crescente em correlacionar esses processos moleculares com a etiologia de algumas doenças bucais, como a periodontite, uma doença imunoinlamatória crônica relacionada a um desequilíbrio da redox celular homeostase. Objetivo: realizar uma revisão narrativa sobre a relaçã o entre a diminuiçã o da capacidade antioxidante salivar induzida pela fumaça do tabaco, periodontite e o uso potencial da farmacologia redox para o tratamento desta patologia. Métodos: uma pesquisa bibliográica foi realizada usando bases de dados como PUBMED (NLM, NIH, NCBI) e SciELO. Resultados: há evidências que relacionam a baixa capacidade antioxidante salivar com o retardo no restabelecimento das condições normais da cavidade oral antes do desenvolvimento da periodontite. Por sua vez, o estado inflamatório associado colabora sinergicamente, causando maior dano tecidual com perda de tecidos de suporte dentário, fenômeno que poderia ser modulado pela açã o da farmacologia redox. Conclusões: a intervençã o com a farmacologia redox poderia atenuar os biomarcadores de progressã o da doença periodontal, constituindo-se em uma ferramenta promissora para ser utilizada em conjunto com estratégias tradicionais de tratamento.


Subject(s)
Humans , Periodontitis/etiology , Periodontitis/drug therapy , Saliva/metabolism , Oxidative Stress , Tobacco Smoking/adverse effects , Antioxidants/therapeutic use , Oxidation-Reduction , Biomarkers , Oxidative Stress/drug effects , Homeostasis
9.
Protein & Cell ; (12): 180-202, 2022.
Article in English | WPRIM | ID: wpr-929176

ABSTRACT

Zn2+ is required for the activity of many mitochondrial proteins, which regulate mitochondrial dynamics, apoptosis and mitophagy. However, it is not understood how the proper mitochondrial Zn2+ level is achieved to maintain mitochondrial homeostasis. Using Caenorhabditis elegans, we reveal here that a pair of mitochondrion-localized transporters controls the mitochondrial level of Zn2+. We demonstrate that SLC-30A9/ZnT9 is a mitochondrial Zn2+ exporter. Loss of SLC-30A9 leads to mitochondrial Zn2+ accumulation, which damages mitochondria, impairs animal development and shortens the life span. We further identify SLC-25A25/SCaMC-2 as an important regulator of mitochondrial Zn2+ import. Loss of SLC-25A25 suppresses the abnormal mitochondrial Zn2+ accumulation and defective mitochondrial structure and functions caused by loss of SLC-30A9. Moreover, we reveal that the endoplasmic reticulum contains the Zn2+ pool from which mitochondrial Zn2+ is imported. These findings establish the molecular basis for controlling the correct mitochondrial Zn2+ levels for normal mitochondrial structure and functions.


Subject(s)
Animals , Caenorhabditis elegans/metabolism , Cation Transport Proteins/genetics , Homeostasis , Mitochondria/metabolism , Zinc/metabolism
10.
Article in English | WPRIM | ID: wpr-929154

ABSTRACT

The dorsal lingual epithelium, which is composed of taste buds and keratinocytes differentiated from K14+ basal cells, discriminates taste compounds and maintains the epithelial barrier. N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotic cells. How METTL3-mediated m6A modification regulates K14+ basal cell fate during dorsal lingual epithelium formation and regeneration remains unclear. Here we show knockout of Mettl3 in K14+ cells reduced the taste buds and enhanced keratinocytes. Deletion of Mettl3 led to increased basal cell proliferation and decreased cell division in taste buds. Conditional Mettl3 knock-in mice showed little impact on taste buds or keratinization, but displayed increased proliferation of cells around taste buds in a protective manner during post-irradiation recovery. Mechanically, we revealed that the most frequent m6A modifications were enriched in Hippo and Wnt signaling, and specific peaks were observed near the stop codons of Lats1 and FZD7. Our study elucidates that METTL3 is essential for taste bud formation and could promote the quantity recovery of taste bud after radiation.


Subject(s)
Animals , Epithelium/metabolism , Homeostasis , Methylation , Methyltransferases/metabolism , Mice , RNA , Taste Buds/metabolism
11.
Article in English | WPRIM | ID: wpr-929153

ABSTRACT

Head and neck squamous cell carcinoma (HNSCC) still lacks effective targeted treatment. Therefore, exploring novel and robust molecular targets is critical for improving the clinical outcome of HNSCC. Here, we reported that the expression levels of family with sequence similarity 64, member A (FAM64A) were significantly higher in HNSCC tissues and cell lines. In addition, FAM64A overexpression was found to be strongly associated with an unfavorable prognosis of HNSCC. Both in vitro and in vivo evidence showed that FAM64A depletion suppressed the malignant activities of HNSCC cells, and vice versa. Moreover, we found that the FAM64A level was progressively increased from normal to dysplastic to cancerous tissues in a carcinogenic 4-nitroquinoline-1-oxide mouse model. Mechanistically, a physical interaction was found between FAM64A and forkhead box protein M1 (FOXM1) in HNSCC cells. FAM64A promoted HNSCC tumorigenesis not only by enhancing the transcriptional activity of FOXM1, but also, more importantly, by modulating FOXM1 expression via the autoregulation loop. Furthermore, a positive correlation between FAM64A and FOXM1 was found in multiple independent cohorts. Taken together, our findings reveal a previously unknown mechanism behind the activation of FOXM1 in HNSCC, and FAM64A might be a promising molecular therapeutic target for treating HNSCC.


Subject(s)
Animals , Carcinogenesis , Cell Line, Tumor , Cell Proliferation , Cell Transformation, Neoplastic , Head and Neck Neoplasms/genetics , Homeostasis , Mice , Squamous Cell Carcinoma of Head and Neck
12.
Article in Chinese | WPRIM | ID: wpr-941006

ABSTRACT

OBJECTIVE@#To explore the mechanism by which inositol-requiring enzyme-1α (IRE1α) regulates autophagy function of chondrocytes through calcium homeostasis endoplasmic reticulum protein (CHERP).@*METHODS@#Cultured human chondrocytes (C28/I2 cells) were treated with tunicamycin, 4μ8c, rapamycin, or both 4μ8c and rapamycin, and the expressions of endoplasmic reticulum (ER) stress- and autophagy-related proteins were detected with Western blotting. Primary chondrocytes from ERN1 knockout (ERN1 CKO) mice and wild-type mice were examined for ATG5 and ATG7 mRNA expressions, IRE1α and p-IRE1α protein expressions, and intracellular calcium ion content using qPCR, Western blotting and flow cytometry. The effect of bafilomycin A1 treatment on LC3 Ⅱ/LC3 Ⅰ ratio in the isolated chondrocytes was assessed with Western blotting. Changes in autophagic flux of the chondrocytes in response to rapamycin treatment were detected using autophagy dual fluorescent virus. The changes in autophagy level in C28/I2 cells overexpressing CHERP and IRE1α were detected using immunofluorescence assay.@*RESULTS@#Tunicamycin treatment significantly up-regulated ER stress-related proteins and LC3 Ⅱ/LC3 Ⅰ ratio and down-regulated the expression of p62 in C28/I2 cells (P < 0.05). Rapamycin obviously up-regulated LC3 Ⅱ/LC3 Ⅰ ratio (P < 0.001) in C28/I2 cells, but this effect was significantly attenuated by co-treatment with 4μ8c (P < 0.05). Compared with the cells from the wild-type mice, the primary chondrocytes from ERN1 knockout mice showed significantly down-regulated mRNA levels of ERN1 (P < 0.01), ATG5 (P < 0.001) and ATG7 (P < 0.001), lowered or even lost expressions of IRE1α and p-IRE1α proteins (PP < 0.01), and increased expression of CHERP (P < 0.05) and intracellular calcium ion content (P < 0.001). Bafilomycin A1 treatment obviously increased LC3 Ⅱ/ LC3 Ⅰ ratio in the chondrocytes from both wild-type and ERN1 knockout mice (P < 0.01 or 0.05), but the increment was more obvious in the wild-type chondrocytes (P < 0.05). Treatment with autophagy dual-fluorescence virus resulted in a significantly greater fluorescence intensity of LC3-GFP in rapamycin-treated ERN1 CKO chondrocytes than in wild-type chondrocytes (P < 0.05). In C28/I2 cells, overexpression of CHERP obviously decreased the fluorescence intensity of LC3, and overexpression of IRE1α enhanced the fluorescence intensity and partially rescued the fluorescence reduction of LC3 caused by CHERP.@*CONCLUSION@#IRE1α deficiency impairs autophagy in chondrocytes by upregulating CHERP and increasing intracellular calcium ion content.


Subject(s)
Animals , Autophagy , Calcium/metabolism , Chondrocytes , Endoplasmic Reticulum/metabolism , Endoribonucleases/pharmacology , Homeostasis , Inositol , Mice , Mice, Knockout , Protein Serine-Threonine Kinases , RNA, Messenger/metabolism , Sirolimus/pharmacology , Tunicamycin/pharmacology
13.
Acta Physiologica Sinica ; (6): 434-442, 2022.
Article in Chinese | WPRIM | ID: wpr-939578

ABSTRACT

The nervous system and the immune system are relatively independent but interactional, and neuro-immune regulation is very important for the respiratory system to resist external harmful stimuli and to maintain homeostasis. Neuro-immune interaction is involved in the occurrence and development of respiratory diseases, and is essential for monitoring and modulating inflammation and tissue repair. This article summaries the neuro-immune regulation of respiratory system and discusses its role in respiratory diseases, aiming to provide a theoretical basis for further understanding the crosstalk between the nervous and immune systems, to explore the underlying mechanism in respiratory diseases, and to provide new thoughts for the prevention and treatment of respiratory diseases.


Subject(s)
Homeostasis , Humans , Immune System , Inflammation , Nervous System , Neuroimmunomodulation , Respiration Disorders
14.
Article in Chinese | WPRIM | ID: wpr-928220

ABSTRACT

Difference of chronotypes makes influence to cognitive performance of individuals in routine duties. In this paper, 55 subjects with different chronotypes were subjected to continuous sleep deprivation for 30 h by using the constant routine protocol, during which core body temperature was measured continuously, and subjective sleepiness self-rating and the performance of selective attention were measured hourly. The results showed that the phase difference of core body temperature has no significant difference, yet the amplitude and term difference among the three chronotypes are significant. There was an advance in phase between subjective sleepiness self-rating and core body temperature, and the self-rating sleepiness of evening type came the latest, and the self-rating sleepiness of morning type dissipated the fastest. The response time of selective attention showed a 2 h phase delay with subjective sleepiness self-rating. And the analysis of core body temperature showed that the later the chronotype was, the greater the phase delay was. The correct rate of selective attention of different chronotypes were inconsistent with delay of subjective sleepiness self-rating and core body temperature. We provide reference for industry, aviation, military, medical and other fields to make a more scientific scheduling/ shifting based on cognitive performance characteristics of different chronotypes.


Subject(s)
Attention/physiology , Circadian Rhythm/physiology , Homeostasis , Humans , Sleep/physiology , Sleep Deprivation , Sleepiness
15.
Med. lab ; 26(2): 141-157, 2022. ilus, Tabs
Article in Spanish | LILACS | ID: biblio-1393214

ABSTRACT

The eosinophil is a cell of the immune system, with an arsenal of substances that can alter the balance that exists in the different organs where they are found. With the advent of monoclonal antibodies, concern about their depletion has become an important turning point in their formulation. For this reason, it is of vital importance to investigate the consequences of the mechanism of action of biological agents, in the short and long term. This review tries to show the role of eosinophils in both homeostasis and disease, and their relationship and interaction with monoclonal drugs in diseases focused on the Th2 profile. It is expected that this article can be useful when making the decision to start treatment with monoclonals, specifically anti-interleukin-5 or against its receptor


Subject(s)
Eosinophils , Pharmaceutical Preparations , Biological Factors , Lymphocyte Depletion , Adrenal Cortex Hormones , Homeostasis
16.
Arq. Asma, Alerg. Imunol ; 5(4): 361-370, out.dez.2021. ilus
Article in English, Portuguese | LILACS | ID: biblio-1399785

ABSTRACT

O corpo humano tende sempre a procurar um estado de homeostase, buscando o equilíbrio entre todos os sistemas. O exercício físico está presente na rotina diária de indivíduos, mesmo com objetivos diferentes, porém a influência no sistema imunológico não é muitas vezes abordada como fator relevante. O sistema imune é responsável por proteger o organismo contra infecções e doenças, podendo ser modulado perante a resposta de exercícios físicos regulares. Tendo em vista que, atualmente, existe uma preocupação maior em tornar e manter a imunidade eficiente, a prática regular e moderada do exercício pode contribuir para uma maior eficácia desse sistema, dessa forma, podendo ser considerada uma proteção ao corpo humano. O objetivo dessa revisão foi sintetizar os dados de estudos presentes na literatura que demonstram a influência do exercício físico na resposta do sistema imunológico, tornando possível compreender as alterações moleculares, fisiológicas, metabólicas e celulares que levam a um tipo específico de resposta do organismo humano.


The human body always tends to seek a homeostasis state, trying to balance all systems. Physical exercise is present in the routine of individuals even with different goals, but the influence in the immune system isnt a relevant factor. The immune system is responsible for protecting the human body against some infections and diseases, and could be modulated in response by some regular physical exercise. At the moment there is a greater concern to keep efficient immunity, a practice of regular and moderate exercise can contribute to a better effectiveness of this system, thus, it can be considered a form of protection to the human body. The objective of this review was to synthesize some data from any studies presented in the literature that demonstrate the influence of physical exercise on the immune system response. Making it possible to understand the molecular mechanisms, physiological, metabolic and cellular changes that turn to a specific type of response in the human body.


Subject(s)
Humans , Exercise , Immune System , Immunity , Dendritic Cells , Immunoglobulins , Killer Cells, Natural , Lymphocytes , Monocytes , Cytokines , Human Body , Chemokines , Protective Factors , Endurance Training , Homeostasis , Leukocytes , Macrophages , Neutrophils
18.
Arq. bras. cardiol ; 117(3): 437-443, Sept. 2021. tab
Article in English, Portuguese | LILACS | ID: biblio-1339189

ABSTRACT

Resumo Fundamento A doença valvar mitral reumatismal (DVMR) é a apresentação mais comum das doenças cardíacas reumáticas (DCR). Os processos de inflamação e fibrose também têm papéis significativos em sua patogênese. Estudos recentes demonstram que os tióis e o tiol-dissulfeto são marcadores de stress oxidativo inéditos e promissores. Objetivos O objetivo deste estudo foi avaliar diferenças entre os níveis de tiol sérico e de tiol-dissulfeto em pacientes com DVMR e no grupo de controle. Métodos Noventa e dois pacientes com DVMR foram cadastrados no estudo. Cinquenta e quatro sujeitos saudáveis, e com correspondência de sexo e idade em relação ao grupo de estudo, também foram incluídos no estudo como um grupo de controle. Foram investigados os níveis de tiol nos pacientes com DVMR e o grupo de controle. Os p-valores menores que 0,05 foram considerados estatisticamente significativos. Resultados Os pacientes com DVMR apresentaram pressão sistólica da artéria pulmonar (PSAP) e níveis de diâmetro do átrio esquerdo (AE) mais altos que os do grupo de controle. Os níveis de tiol nativo (407±83 μmol/L vs. 297±65 μmol/L, p<0,001) e tiol total (442±82 μmol/L vs. 329±65 μmol/L, p<0,001) são mais altos no grupo de controle. Níveis de dissulfeto (16,7±4,9 μmol/L vs. 14,8±3,7 μmol/L, p=0,011) são mais altos no grupo de pacientes com DVMR. Foi identificada uma correlação positiva entre as razões dissulfeto/tiol nativo e dissulfeto/tiol total com PSAP, diâmetro de AE, e gravidade da EMi. A razão dissulfeto/tiol total é significativamente mais alta em pacientes com EMi grave que em pacientes com EMi leve a moderada. Conclusões Até onde se sabe, este é o único estudo que avaliou a homeostase tiol/dissulfeto como um preditor inédito, que está relacionado de forma mais próxima à DVMR e à gravidade da EMi.


Abstract Background Rheumatic mitral valve disease (RMVD) is the most common presentation of rheumatic heart disease (RHD). Inflammation and fibrosis processes also play significant roles in its pathogenesis. Recent studies showed that thiols and thiol-disulfide are promising novel oxidative stress markers. Objectives The present study aimed to evaluate differences in the serum thiol and thiol-disulfide levels in patients with RMVD and the control group. Methods Ninety-two patients with RMVD were enrolled in the study. Fifty-four healthy subjects, age, and gender-matched with the study group, were also included in the study as a control group. This study investigated thiol levels in patients with RMVD and the control group. P-values lower than 0.05 were considered statistically significant. Results The patients with RMVD presented higher systolic pulmonary artery pressure (SPAP) and left atrial (LA) diameter levels than the control group. Native thiol (407±83 μmol/L vs. 297±65 μmol/L, p<0.001) and total thiol (442±82 μmol/L vs. 329±65 μmol/L, p<0.001) levels were higher in the control group. Disulfide (16.7±4.9 μmol/L vs. 14.8±3.7 μmol/L, p=0.011) levels were higher in the group of patients with RMVD. A positive correlation was found between disulfide/native and disulfide/total thiols ratio with SPAP, LA diameter, and MS severity. Disulfide/total thiols ratio was significantly higher in patients with severe MS than with mild to moderate MS patients. Conclusions To the best of our knowledge, this is the only study of its kind that has evaluated thiol/disulfide homeostasis as a novel predictor, which was more closely related to RMVD and the severity of MS.


Subject(s)
Humans , Rheumatic Heart Disease , Disulfides , Sulfhydryl Compounds , Biomarkers , Case-Control Studies , Oxidative Stress , Healthy Volunteers , Homeostasis , Mitral Valve
19.
Rev. Assoc. Med. Bras. (1992) ; 67(7): 1026-1032, July 2021. tab, graf
Article in English | LILACS | ID: biblio-1346936

ABSTRACT

SUMMARY OBJECTIVE: Many chronic diseases such as malignancy, cardiovascular diseases, endothelial dysfunction, and autoimmune diseases, which have been shown to be related to vitamin D in various studies; have similar relations with CTRP-9, TNFα, and thiol-disulfide hemostasis. We aimed to contribute to the literature by evaluating the relationship between CTRP-9, TNFα, and thiol-disulfide hemostasis and vitamin D levels, which we thought may have some effects on the pathogenesis of vitamin D deficiency. METHODS: In our study, 78 female volunteers older than 18 years were included. Volunteers were divided into three groups according to the reference values of vitamin D levels. Biochemical parameters, CTRP-9, TNFα, and thiol/disulfide hemostasis tests taken from all volunteers were studied. RESULTS: In this study, there was a significant difference in CTRP-9, TNFα, total thiol (TT), native thiol (NT), DIS (disulfide), TT/DIS, and NT/DIS levels in vitamin D groups (p<0.05). There was a significant negative correlation between vitamin D and TNFα and DIS, while a significant positive correlation was found with CTRP-9, TT, NT, TT/DIS, and NT/DIS (p<0.05). CONCLUSIONS: It was determined that vitamin D deficiency causes a significant decrease in CTRP-9 level and a significant increase in TNFα level, as well as an increase in thiol/disulfide hemostasis in favor of disulfide, which may be a risk factor for increased oxidative stress. We considered that these changes may play mediator roles for many chronic diseases and metabolic disorders that are increasing in frequency due to vitamin D deficiency.


Subject(s)
Humans , Female , Tumor Necrosis Factor-alpha , Disulfides , Sulfhydryl Compounds , Vitamin D , Biomarkers , Oxidative Stress , Hemostasis , Homeostasis
20.
Int. j. high dilution res ; 20(2/3): 24-33, June 4, 2021.
Article in English | LILACS, HomeoIndex | ID: biblio-1396356

ABSTRACT

Anamnesis and homeopathic repertorization are essential steps for collecting symptoms and selecting the suitable medicine to cure vegetables presenting pathologies or changes in homeostasis. Aims: The objective of this study was to carry out observation, anamnesis, and homeopathic repertorization of bell pepper plants and their cultivation environment and select thesimillimummedicine for the culture. Methods The study was performed at the Research and Extension Center in Agroecology of Ressacada Experimental Farm -Center of Agrarian Sciences, Federal University of Santa Catarina,Florianópolis, Brazil. The selection of symptoms was based on the characteristics of the diseases observed in the plants and modalized by evidenced characteristics in the environment. Symptomswere chosen by the mechanical method without hierarchization and a master symptom, and after their analogy with the symptoms described in the Homeopathic Materia Medica. Results The choice forCalcarea carbonicaandSulphurdrugs was based on the number of covered symptoms and the score obtained during the digital repertorization performed by the software HomeoPro®. The chosen dilution, 30 CH, was based on the miasmatic magnitude presented by the symptoms. Conclusion: The analogy of symptoms found in the agricultural organism with the symptoms described in the Homeopathic Materia Medica presents a promising path for choosing medicine to cure diseases in vegetables. The choice of the appropriate homeopathic medicine for crops must represent the symptomatic totality presented by the plant and by the environment in the homeopathic repertorization.


Subject(s)
Capsicum , Repertorization , Solanaceae , Homeostasis
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