ABSTRACT
Introducción: La leptospirosis es una zoonosis que cons-tituye un problema emergente de salud pública. La insufi-ciencia renal, plaquetopenia y compromiso respiratorio se describen como predictores de mortalidad.Objetivos: Describir características clínicas, radiológicas y de laboratorio de individuos hospitalizados por leptos-pirosis y evaluar los predictores de mala evolución clínica (MEC).Materiales y métodos: Estudio de cohorte de inclusión ambispectiva de pacientes con leptospirosis internados en un hospital de la ciudad de Santa Fe entre 1997 y 2022. Se definió MEC como la admisión a Unidad de Cuidados Intensivos (UCI), requerimiento de asistencia respiratoria mecánica (ARM) y/o muerte. Se utilizaron las pruebas de Chi2, test T de Student o la U de Mann-Whitney, según co-rrespondiera. Se construyó una regresión logística binaria con las variables con p<0,05.Resultados: 101 pacientes, 87,1% (n=88) hombres, media-na de edad de 29 (RIC 20-44) años. La fiebre fue el síntoma más frecuente [83,2% (n=84)], seguido del compromiso di-gestivo [62,4% (n=63)]. Las alteraciones de laboratorio más frecuentes fueron: eritrosedimentación elevada [91,9% (n=79)] y leucocitosis [61% (n=61)]. Se observó MEC en el 25,7% (n=26). El 25,7% (n=26) fue admitido en UCI, el 13,9% (n=14) requirió ARM y el 5% (n=5) falleció. La presencia de plaquetopenia (OR=13,3, IC95% 2-80), las alteraciones en la radiografía de tórax (OR=33,5, IC95% 5-225) y la ausencia de cefalea (OR=6,8, IC95% 1-32) fueron predictores inde-pendientes de MEC.Conclusiones: En concordancia con la bibliografía, la afec-tación pulmonar y plaquetopenia son factores de riesgo para la mala evolución clínica. En nuestra serie, la cefalea constituyó un síntoma protector
Introduction: Leptospirosis is an emerging zoonotic di-sease that poses a public health problem. Renal failu-re, thrombocytopenia, and respiratory involvement have been described as predictors of mortality.Objectives: To describe the clinical, radiological, and la-boratory characteristics of hospitalized individuals with leptospirosis and evaluate predictors of poor clinical outcomes (PCO).Materials and methods: A prospective cohort study was conducted including patients with leptospirosis admit-ted to a hospital in the city of Santa Fe between 1997 and 2022. PCO was defined as admission to the Intensive Care Unit (ICU), requirement for mechanical respiratory assistance (MRA), and/or death. The chi-square test, Student>s t-test, or Mann-Whitney U test were used as appropriate. A binary logistic regression was performed with variables having p<0.05.Results: Out of the 101 patients included, 87.1% (n=88) were male, with a median age of 29 (IQR 20-44) years. Fever was the most common symptom [83.2% (n=84)], followed by digestive involvement [62.4% (n=63)]. The most frequent laboratory abnormalities were elevated erythrocyte sedimentation rate [91.9% (n=79)] and leuko-cytosis [61% (n=61)]. PCO was observed in 25.7% (n=26) of patients, with 25.7% (n=26) admitted to the ICU, 13.9% (n=14) requiring MRA, and 5% (n=5) resulting in death. The presence of thrombocytopenia (OR=13.3, 95% CI 2-80), abnormalities in chest X-rays (OR=33.5, 95% CI 5-225), and absence of headache (OR=6.8, 95% CI 1-32) were predictors of PCO. Conclusions: Consistent with the literature, pulmonary involvement and thrombocytopenia are independent risk factors for poor clinical outcomes. In our series, the pre-sence of headache was a protective symptom
Subject(s)
Humans , Male , Female , Endemic Diseases/prevention & control , Hospitalization , Leptospira/pathogenicity , Leptospirosis/mortalityABSTRACT
Introduction@#ILeptospirosis is an important zoonotic disease commonly found in tropical or sub-tropical countries. The most severe form is Weil's syndrome which presents with jaundice, renal failure, and bleeding diatheses. Although jaundice occurs in 38% of patients with leptospirosis, no studies in Asia have focused on the liver biochemical profile of these patients. Characterization of liver biochemical profile and ultrasonographic findings may shed more light on the disease process. Identification of liver biochemical parameters that portend a poor prognosis may also allow for early aggressive intervention. @*Objective@#To describe the liver biochemical profile and liver ultrasonographic findings in adult patients with laboratoryconfirmed leptospirosis, admitted at a tertiary hospital in Manila, Philippines. The association of clinical and laboratory features with clinical outcomes (i.e., severe liver injury, Weil’s syndrome, and mortality) was also investigated. @*Methods@#This retrospective cross-sectional study reviewed all available cases of adult patients with laboratoryconfirmed leptospirosis admitted in the Philippine General Hospital from January 2009 to August 2018. The clinical features, liver biochemical profiles, and ultrasound findings were recorded and analyzed. Comparison between the means of each group based on clinical outcome (i.e., mortality, Weil’s syndrome) was done via Students’ t-test for continuous variables, and calculation of the Odds Ratio for categorical variables.@*Results@#Total and direct bilirubin levels were elevated in patients with leptospirosis compared to serum aminotransferases and alkaline phosphatase levels which were only mildly elevated. Abdominal ultrasound showed typically un-enlarged livers with normal parenchymal echogenicity, normal spleens, and non-dilated biliary trees. Dyspnea was associated with an increased odds for mortality. Although jaundice was present in 39.5% of patients and significantly associated with severe liver injury, this was not associated with mortality. Liver biochemical test values did not differ among patients who expired and those who survived to discharge. The presence of myalgia and abdominal pain increased the odds for Weil's syndrome.@*Conclusion@#To date, no local studies have fully described the liver biochemical profile of patients with leptospirosis. Our findings are compatible with previous studies showing that leptospirosis typically presents with predominantly elevated direct bilirubin from cholestasis and systemic infection. Contrary to previous literature, however, our study found no association between jaundice and mortality.
Subject(s)
LeptospirosisABSTRACT
A leptospirose é uma doença infecciosa febril de início abrupto, com espectro clínico variando desde casos leves até formas graves. Causada por bactéria do gênero leptospira e da classe espiroquetas, com mais de 14 espécies patogênicas, sendo a mais importante a espécie Leptospira interrogans. Está presente em áreas urbanas e rurais e de proliferação relacionada a fatores ambientais que atingem pessoas e animais. Esse boletim tem como objetivo descrever a epidemiologia dos casos de leptospirose notificados em um hospital referência em infectologia no Estado de Goiás. Trata-se de um estudo epidemiológico, observacional e descritivo de caráter retrospectivo, com abordagem quantitativa. Foram avaliados os casos notificados de leptospirose em pacientes de todas as faixas etárias que foram notificados no Hospital Estadual de Doenças Tropicais
Leptospirosis is a febrile infectious disease with an abrupt onset, with a clinical spectrum ranging from mild cases to severe forms. Caused by bacteria of the genus Leptospira and the spirochete class, with more than 14 pathogenic species, the most important being the Leptospira interrogans species. It is present in urban and rural areas and has a proliferation related to environmental factors that affect people and animals. This bulletin aims to describe the epidemiology of leptospirosis cases reported in a reference hospital for infectious diseases in the State of Goiás. It is an epidemiological, observational and descriptive study of a retrospective nature, with a quantitative approach. Reported cases of leptospirosis in patients of all age groups who were reported at the State Hospital for Tropical Diseases were evaluated
Subject(s)
Humans , Leptospirosis/epidemiologyABSTRACT
A leptospirose é uma doença infectocontagiosa, causada por espiroquetas antigenicamente distintas da bactéria Leptospira interrogans. Os cães representam um importante elo na transmissão da leptospirose, visto que, aparentemente sadios, podem albergar leptospiras e eliminá-las no meio ambiente. Com o objetivo de se conhecer a prevalência sorológica anti-leptospírica em cães de Cruz das Almas - BA e os fatores de risco para essa zoonose, foram coletadas amostras de sangue de 200 cães. A prova diagnóstica utilizada foi a Soroaglutinação Microscópica, testando-se 19 sorovares. Das 200 amostras testadas, 60 foram reagentes. Os resultados demonstram que a sororeatividade dos cães representam risco de disseminação da leptospirose para outros animais e para o ser humano.
Leptospirosis is an infectious disease, caused by spirochetes antigenically distinct from the bacteria Leptospira interrogans. Dogs represent an important link in the transmission of leptospirosis, since, apparently healthy, they can house leptospires and eliminate them in the environment. In order to know the anti-leptospiric serological prevalence in dogs from Cruz das Almas - BA and the risk factors for this zoonosis, blood samples were collected from 200 dogs. The diagnostic test used was Microscopic Soroagglutination, testing 19 serovars. Of the 200 samples tested, 60 were reagents. The results demonstrate that the seroreactivity of dogs represents a risk of spreading leptospirosis to other animals and to humans.
Subject(s)
Animals , Dogs , Seroepidemiologic Studies , Prevalence , Dogs/immunology , Bacterial Zoonoses , Leptospira , Leptospirosis/veterinaryABSTRACT
Introdução: A leptospirose é considerada um problema de saúde pública com grande impacto social e econômico. A doença foi citada como duplamente omissa, uma vez já classificada na literatura internacional como doença tropical negligenciada (DTN), ainda conta com a baixa atenção governamental. Tal fato, pode estar associado ao adoecimento por essa enfermidade atingir majoritariamente a porção pobre e marginalizada da sociedade. Objetivos: O presente estudo objetiva avaliar a associação entre determinantes em saúde e adoecimento por leptospirose nos municípios do estado de Minas Gerais no período de 2010 a 2019. Metodologia: Trata-se de um estudo ecológico, no qual as unidades de análise foram os 853 municípios do estado de Minas Gerais. Foram utilizados dados secundários agregados não nominais entre os anos de 2010 e 2019. Foi utilizado o Modelo Binomial Negativo com Inflação de Zeros como técnica de análise multivariada, considerando a incidência por leptospirose em 2016 como desfecho e os indicadores do Índice Mineiro de Responsabilidade Social de 2016 ou em data mais próxima disponível como variáveis explicativas Resultados: Os resultados do modelo múltiplo demonstraram que os fatores que permaneceram associados à incidência de leptospirose foram os índices de Saúde, Educação, Renda e Emprego e a variável Infraestrutura Urbana. Segundo os coeficientes estimados, o índice de saúde do IMRS foi associado a um aumento da incidência de leptospirose. Por outro lado, os demais fatores índice de educação, renda e emprego e a variável infraestrutura urbana estiveram associados a menores taxas da doença, agindo assim, como fatores de proteção ao adoecimento. Conclusão: Há uma forte associação da leptospirose com a pobreza no estado de Minas Gerais. O investimento em infraestrutura urbana e garantia de condições sanitárias favoráveis para a população, bem como melhora da propagação de informação e educação reduziria o impacto da doença na população pobre e desassistida.
Introduction: Leptospirosis is considered a public health problem with great social and economic impact. The disease was cited as doubly omitted, since it is already classified in the international literature as a neglected tropical disease (NTD), it still has low government attention. This fact may be associated with the illness caused by this disease, which mostly affects the poor and marginalized portion of society. Objectives: This study aims to evaluate the association between health determinants and the illness from leptospirosis in the municipalities of the state of Minas Gerais from 2010 to 2019. Methodology: This is an ecological study and the units of analysis were the 853 municipalities in the state of Minas Gerais. Non-nominal aggregated secondary data between the years 2010 and 2019 were used. The Negative Binomial Model with Zero Inflation was used as a multivariate analysis technique, considering the incidence of leptospirosis in 2016 as the outcome and the IMRS indicators of 2016 or on a date closest available as explanatory variables Results: The results of the multiple model showed that the factors that remained associated with the incidence of leptospirosis were the Health, Education, Income and Employment indexes and the Urban Infrastructure variable. According to the estimated coefficients, the IMRS health index was associated with an increased incidence of leptospirosis. On the other hand, the other factors education, income and employment index and the urban infrastructure variable were associated with lower rates of the disease, thus acting as protective factors against illness. Conclusion: There is a strong association between leptospirosis and poverty in the state of Minas Gerais. Investment in urban infrastructure and guaranteeing favorable sanitary conditions for the population, as well as improving the dissemination of information and education would reduce the impact of the disease on the poor and underserved population. Descriptors: Social Determinants of Health; Zoonosis Surveillance; Leptospirosis, Social Vulnerability, Ecological Studies.
Subject(s)
Social Determinants of Health , Social Vulnerability , Zoonosis Surveillance , Leptospirosis , Tropical Medicine , Public Health , Academic Dissertation , Ecological StudiesABSTRACT
Coletânea de 13 boletins publicados no site da Secretaria de Estado da Saúde de Goiás, que se destacaram por descrever o perfil de morbimortalidade da unidade e apresentar temas relevantes para a população do Estado de Goiás. Trata-se de um estudo descritivo, realizado com dados do período de janeiro a março de 2023, com informações provenientes das declarações de óbitos e prontuário eletrônico do paciente
Collection of 13 bulletins published on the Goiás State Department of Health website, which stood out for describing the unit's morbidity and mortality profile and presenting relevant themes for the population of the State of Goiás. This is a descriptive study, carried out with data from January to March 2023, with information from death certificates and the patient's electronic medical record
Subject(s)
Humans , Male , Female , Pregnancy , Infant, Newborn , Adult , Middle Aged , Aged , Epidemiology/statistics & numerical data , Tuberculosis/epidemiology , Violence/statistics & numerical data , Accidents, Occupational/statistics & numerical data , Syphilis/epidemiology , Mortality , Chagas Disease/epidemiology , Severe Acute Respiratory Syndrome/epidemiology , Dengue/epidemiology , Live Birth , COVID-19/epidemiology , Hepatitis/epidemiology , Leptospirosis/epidemiology , Meningitis/epidemiologyABSTRACT
Worldwide, leptospirosis is the most highly prevalent zoonosis. Although the wide range of clinical manifestations of leptospirosis in humans is well-documented, knowledge of the mechanisms through which this pathogen causes kidney disease remains limited. This narrative review of the scientific literature presents experimental studies of pathophysiology and kidney disease in leptospirosis, both in humans and animals, and the results show that virulence factors are involved in kidney damage by inducing interstitial tubular nephritis, which is the most frequent pathological manifestation, additionally, to the acute non-oliguric renal lesion with hypokalemia, and loss of magnesium and sodium. Finally, it is concluded that in leptospirosis, the initial lesion in the kidney is caused by damage to the cell membrane of the proximal tubular region cells by pathogenic Leptospira virulence factors, thus exacerbating the immune response
Subject(s)
Humans , Leptospira , Leptospirosis , Cells , Kidney DiseasesABSTRACT
La leptospirosis es una enfermedad zoonótica prevalente y generalizada de la cual no existen cifras fiables de incidencia mundial. Es una enfermedad con subregistro que puede generar una cifra importante de morbimortalidad. La incidencia de afectación pulmonar en la leptospirosis varía del 20 % al 70 %, y la hemorragia alveolar que se presenta con disnea y hemoptisis es su principal manifestación pulmonar, generando una mortalidad que puede ser mayor al 50 % si no se detecta y maneja a tiempo. Presentamos el caso de un paciente de 30 años de edad con sintomatología respiratoria y fiebre que hizo pensar en COVID-19 por las imágenes radiológicas y por época de pandemia, con antecedente de ser recolector de botellas, con descenso brusco de hemoglobina sin un sitio preciso de sangrado, considerándose como posibilidad hemorragia alveolar. Se le realizó serología por Leptospira siendo esta positiva y pudo ser tratado oportunamente. En conclusión, un buen interrogatorio sobre las actividades diarias junto con un buen juicio clínico e interpretación de exámenes laboratoriales nos debe hacer sospechar en hemorragia alveolar por leptospirosis.
Leptospirosis is a prevalent and generalized zoonotic disease, for which there are no reliable figures of world incidence. It is an underreported disease that can generate a significant number of morbidity and mortality. The incidence of pulmonary involvement in leptospirosis varies from 20% to 70%, and alveolar hemorrhage that presents with dyspnea and hemoptysis is the main pulmonary manifestation in leptospirosis, generating mortality that can be greater than 50% if it is not detected and drive on time. We present the case of a 30-year-old patient with respiratory symptoms and fever that suggested Covid 19 due to radiological images and due to the pandemic, with a history of being a bottle collector, who due to the sudden drop in hemoglobin without a precise site of bleeding was thought to be alveolar hemorrhage, serology was performed for leptospira, which was positive, and he could be treated in a timely manner. In conclusion, a good questioning about daily activities together with good clinical judgment and interpretation of laboratory tests should make us suspect alveolar hemorrhage due to leptospirosis.
Subject(s)
Humans , Zoonoses , Leptospirosis , Mortality , Fever , HemorrhageABSTRACT
Introduction: Rodents are potential transmitters of Leptospira spp. In the municipality of Villavicencio, Colombia, leptospirosis is a disease that, although notifiable, is still underreported. In this region, rodent species that can host pathogenic leptospira remain unknown. Objective: To detect the presence of Leptospira spp. through molecular analysis in rodents (Rodentia) from peri-urban and rural areas belonging to the municipality of Villavicencio in Colombia. Methods: Peri-urban and rural areas of the townships belonging to Villavicencio municipality were selected for sampling. These areas presented similar ecological conditions: they were near water bodies and peridomiciliary areas, and some of them included fields of agricultural crops. Rodents´ kidneys were removed and frozen in liquid nitrogen. DNA was extracted using a commercial kit and subsequently amplified through conventional polymerase chain reaction. Results: The rodent species collected were: Rattus rattus, Mus musculus, Zygodontomys brevicauda, Oligoryzomys sp, Hylaeamys (formerly Oryzomys) and Proechimys cf. oconnelli. Leptospira DNA was amplified in six rodents and the purified amplicons were sent to Macrogen Inc. (Seoul, Korea) for sequencing. The alignment analysis of the sequenced products demonstrated 98.64 percent of coverage and identity with Leptospira interrogans. Conclusions: This is the first study carried out on wild and synanthropic rodents in the municipality of Villavicencio. The incidence of leptospirosis raises the alarm due to the important role of these small mammals in the transmission of this zoonosis, which is considered the second cause, after dengue, of undifferentiated febrile illness in Villavicencio(AU)
Introducción: Los roedores son potenciales transmisores de Leptospira spp. En el municipio de Villavicencio, Colombia, la leptospirosis es una enfermedad que, aunque debe notificarse obligatoriamente, sigue subreportada. En esta región, algunas especies de roedores pueden ser reservorios de leptospiras patógenas, situación que se desconoce. Objetivo: Detectar la presencia de Leptospira spp. a través del análisis molecular en roedores (Rodentia) de áreas periurbanas y rurales del municipio de Villavicencio, Colombia. Métodos: Para el trampeo se seleccionaron áreas periurbanas y rurales de las veredas pertenecientes al municipio de Villavicencio. Las áreas escogidas presentaban condiciones ecológicas similares: cerca de cuerpos de agua y áreas peridomiciliarias; algunas de ellas localizadas en campos de cultivos de la agricultura. Se extirparon los riñones de los roedores y se conservaron en nitrógeno líquido. Se extrajo el ADN usando un estuche comercial y posteriormente se amplificó mediante reacción en cadena de la polimerasa convencional. Resultados: Las especies de roedores colectadas fueron: Rattus rattus, Mus musculus, Zygodontomys brevicauda, Oligoryzomys sp., Hylaeamys (ahora Oryzomys) y Proechimys oconnelli. El ADN de leptospira se amplificó en seis roedores y los amplicones purificados se enviaron a Macrogen Inc. (Seoul, Korea) para secuenciación. El análisis de alineamiento de los productos secuenciados demostró un 98,64 por ciento de cobertura e identidad con Leptospira interrogans. Conclusiones: Este es el primer estudio llevado a cabo en roedores silvestres y sinantrópicos en el municipio de Villavicencio. La incidencia de la leptospirosis genera una alarma con respecto a la importancia del papel de esos pequeños mamíferos en la transmisión de esta zoonosis, la cual es la segunda causa de los síndromes febriles indiferenciados en Villavicencio, después del dengue(AU)
Subject(s)
Humans , Leptospira/pathogenicity , Leptospirosis/prevention & control , ColombiaABSTRACT
Introducción: la leptospirosis es una enfermedad infecciosa producida por espiroquetas del género Leptospira. Se disemina a través de la orina de animales domésticos, con mayor frecuencia por roedores. En República Dominicana se necesitan pruebas confiables para el diagnóstico en etapas tempranas de la enfermedad. En el presente trabajo se aborda la validez diagnóstica del PCR en Tiempo Real y del IGM (INMUNODOT) en comparación con la prueba de Microaglutinación (MAT). Materiales y métodos: se realizó un estudio observacional, descriptivo, de corte transversal con 69 pacientes admitidos en el Hospital Regional "José María Cabral y Báez" con diagnóstico presuntivo de leptospirosis desde el 2010 hasta el 2012. Resultados: del total de los casos (69), la mayoría fueron del sexo masculino (94.2 %), menores de 49 años (79.4 %) y provenían de la provincia de Santiago (58 %). La mortalidad fue de 52.1 % de los cuales 52.3 % fueron reportados positivos para Leptospira y 47.6 % resultaron negativos según el MAT. Al comparar los resultados de PCR en relación al MAT se obtuvo una sensibilidad de 27.3 % y una especificidad de 80 %. Los resultados del PCR y del Immunodot fueron equivalentes. Conclusión: en el presente trabajo la realización del PCR en sangre, después del 5to día de inicio de la enfermedad, no demostró ser mejor que la Inmunodot para la detección temprana de la enfermedad, al contrastarlos con el resultado del MAT. En República Dominicana, los casos hospitalizados con diagnóstico presuntivo de leptospirosis permanecen con muy alta mortalidad. Por tanto, es prioritario optimizar el diagnóstico y el tratamiento de estos casos. En este estudio, los casos confirmados con Leptospirosis que fallecieron indican que debe actualizarse el protocolo de tratamiento y asegurarse que pueda implementarse. Por otra parte, los casos no confirmados fallecidos exigen investigar otras causas de enfermedad como la Infección por Hanta Virus.
Introduction: Leptospirosis is an infectious disease caused by spirochaetes of the genus Leptospira. It spread through the urine of domestic animals most frequently in rodents. Need reliable tests to diagnose in early stages of the disease and it has been proposed the use of the PCR in real-time as an option. The present work deals with the diagnostic real-time PCR and IGM (INMUNODOT) in comparison with the Microagglutination (MAT) test. Materials and methods: An observational, descriptive, cross-sectional study with 69 patients admitted as possible Leptospirosis in the Hospital "Jose Maria Cabral y Báez" of Santiago from 2010 to 2012. Results: Of the total cases (69), most were male (94.2%), under 49 years of age (79.4%) coming from the province of Santiago (58%). Mortality was 52.1% of which 52.3% were reported positive for leptospirosis and 47.6% were negative according to the MAT. To compare the results of PCR in relation to the MAT was obtained a sensitivity of 27.3% and a specificity of 80%. The results of the PCR and immunodot were equivalent. Conclusion: In this study, the realization of the PCR in blood after the 5th day of the disease not proved to be better than the Inmunodot for the early detection of the disease. Is necessary to evaluate cases less than 5th day. In the Dominican Republic, hospitalized with a presumptive diagnosis of Leptospirosis cases remain with very high mortality. Therefore, it is important to optimize the diagnosis and treatment of these cases. In this study, confirmed cases with Leptospirosis who died, indicate that you must upgrade the treatment protocol and ensure that it can be implemented. On the other hand, the deceased not confirmed cases require to investigate other causes of disease as the Hanta Virus infection.
Subject(s)
Leptospirosis , Humans , Cross-Sectional Studies , Dominican Republic , MenABSTRACT
Introducción: La leptospirosis es una zoonosis emergente, endémica en Colombia, que afecta tanto animales domésticos como silvestres. Es considerada de riesgo laboral, ya que la transmisión al ser humano está asociada a la exposición con animales o ambientes infectados. En el departamento de Nariño, la producción de cuyes para el consumo humano se realiza en sistemas de crianza tradicionales que podrían favorecer la infección por Leptospira interrogans en esta especie. Objetivo: Detectar molecularmente la infección natural por especies patógenas del género Leptospira en cuyes que son destinados para el consumo humano en el municipio de Pasto. Materiales y métodos: Se tomaron 270 muestras de tejido renal en cuyes sacrificados en dos mataderos. Las muestras fueron analizadas mediante Reacción en Cadena de la Polimerasa (PCR) convencional y coloración diferencial de Warthin Starry (W-S). Resultados: En la evaluación de las 270 muestras, 4 (1,5%) fueron positivas para PCR y una de las muestras demostró la presencia de Leptospira bajo tinción W-S. Conclusiones: Mediante el uso de técnicas moleculares se evidenció L. interrogans en el tejido renal de Cavia porcellus. La circulación del patógeno en esta población representa un riesgo de infección para humanos y animales domésticos en contacto con estos sistemas productivos.
Introduction: Leptospirosis is an emerging zoonosis that is endemic in Colombia and affects both domestic and wild animals. It is considered an occupational risk since human transmission is associated with exposure to infected animals or environments. In the department of Nariño, the production of guinea pigs for human consumption applies traditional rearing systems that could cause animals to get infected with Leptosipira interrogans. Objective: To molecularly identify natural infection by pathogenic species of the genus Leptospira in guinea pigs used for human consumption in the municipality of Pasto (Colombia). Materials and methods: 270 kidney tissue samples were taken from guinea pigs slaughtered in two facilities. Samples were analyzed through conventional polymerase chain reaction (PCR) and Warthin Starry (W-S) differential staining. Results: While 4 (1.5%) out of the 270 samples were categorized as positive using PCR, only 1 sample showed the presence of Leptospira through W-S staining. Conclusions: Molecular techniques were useful to identify L. interrogans in kidney tissue of Cavia porcellus. Dissemination of this pathogen within this population represents an infection risk for humans and domestic animals that are in close proximity to these productive systems.
Subject(s)
Humans , Animals , Guinea Pigs , Zoonoses , Guinea Pigs , Leptospira interrogans , Leptospirosis , Animal DiseasesABSTRACT
Resumo A leptospirose é uma zoonose que apresenta potencial epidêmico, principalmente após fortes chuvas que acarretam inundações, alagamentos e enxurradas. Algumas características da região costeira de Santa Catarina, localizada no Sul do Brasil, influenciam nesses processos. Portanto, a partir do estudo da leptospirose nos seis municípios do estado com as maiores incidências e picos epidêmicos de 2000 a 2015, buscou-se conhecer a tendência dessa doença e as variáveis climáticas e ambientais associadas à sua ocorrência, ajustando dois modelos com resposta binomial negativa. As maiores incidências foram encontradas em 2008 e 2011, com picos no mesmo mês ou no posterior aos eventos de desastres. A incidência apresentou forte comportamento sazonal, sendo maior nos meses do verão. Observou-se tendência de queda na incidência dos municípios estudados, estimada em 3,21% ao ano. Os fatores climáticos e ambientais mais fortemente associados foram o número de dias de chuva, a temperatura máxima e a presença de enxurrada e de inundação, com diferentes impactos entre os municípios. Houve interações significativas, indicando que o efeito de inundações na incidência não é o mesmo em todos os municípios e que as diferenças nas incidências entre os municípios dependem da ocorrência ou não de inundações.
Abstract Leptospirosis is a zoonosis with epidemic potential, especially after heavy rainfall causing river, urban and flash floods. Certain features of Santa Catarina's coastal region influence these processes. Using negative binomial regression, we investigated trends in the incidence of leptospirosis in the six municipalities with the highest epidemic peaks between 2000 and 2015 and the climatic and environmental variables associated with the occurrence of the disease. Incidence was highest in 2008 and 2011, and peaks occurred in the same month or month after disasters. Incidence showed a strong seasonal trend, being higher in summer months. There was a decrease trend in incidence across the six municipalities (3.21% per year). The climatic and environmental factors that showed the strongest associations were number of rainy days, maximum temperature, presence of flash floods, and river flooding. The impact of these variables varied across the municipalities. Significant interactions were found, indicating that the effect of river flooding on incidence is not the same across all municipalities and differences in incidence between municipalities depend on the occurrence of river flooding.
Subject(s)
Humans , Animals , Zoonoses , Leptospirosis/epidemiology , Rain , Brazil/epidemiology , IncidenceABSTRACT
A leptospirose é uma zoonose de distribuição mundial que pode acometer cães e ser altamente letal para a espécie. No Brasil, tal enfermidade tem caráter endêmico na espécie canina e configura-se como um sério problema de saúde pública. Objetivou-se verificar a ocorrência dos sorogrupos/sorovares de Leptospira spp. que mais acometem cães com suspeita clínica de leptospirose na cidade de Santa Maria RS, Brasil, analisando sua titulação de anticorpos. No estudo, utilizou-se os laudos dos exames de soroaglutinação microscópica (SAM) para leptospirose de 218 cães provenientes da cidade de Santa Maria RS durante o período de janeiro de 2015 a dezembro de 2019. Todos os laudos foram emitidos pelo Laboratório de Leptospirose (LabLepto - UFSM). Das 218 amostras de soro processadas nos cinco anos, 101 (46,33%) resultaram positivas para, pelo menos, um sorogrupo/sorovar testado. Este estudo demonstrou maior ocorrência do sorogrupo Icterohaemorrhagiae (53,37%) contemplado pelas sorovares Copenhageni e Icterohaemorrhagiae, com 49 (30,06%) e 38 (23,31%) soros reagentes, respectivamente. As titulações variaram de 100 a 6400, sendo que a mais recorrente encontrada nas amostras deste estudo foi 100, representando 47,85% dos títulos de anticorpos. Os dados obtidos neste estudo são de grande valia para o conhecimento dos sorogrupos/sorovares circulantes na região e sua epidemiologia podendo, inclusive, auxiliar para futuras formulações vacinais considerando sorogrupos/sorovares mais frequentemente detectados, contribuindo, assim, com a saúde pública.
Leptospirosis is a worldwide distribution zoonosis that can affect dogs and be highly lethal for the species. In Brazil, this disease is endemic in the canine species and represents a serious public health problem. The aim of this study was to verify the occurrence of Leptospira spp. that most affect dogs with clinical suspicion of leptospirosis in the city of Santa Maria RS, Brazil, analyzing their antibody titers. In the study, we used the reports of microscopic agglutination tests (MAS) for leptospirosis in 218 dogs from the city of Santa Maria - RS during the period from January 2015 to December 2019. All reports were issued by the Leptospirosis Laboratory (LabLepto - UFSM). Of the 218 serum samples processed over the five years, 101 (46.33%) were positive for at least one serogroup/serovar tested. This study showed a higher occurrence of serogroup Icterohaemorrhagiae (53.37%) covered by serovars Copenhageni and Icterohaemorrhagiae, with 49 (30.06%) and 38 (23.31%) reagent sera, respectively. The titers ranged from 100 to 6400, and the most recurrent found in the samples in this study was 100, representing 47.85% of the antibody titers. The data obtained in this study are of great value for understanding the serogroups/serovars circulating in the region and their epidemiology, and may even contribute to future vaccine formulations considering the most frequently detected serogroups/serovars, thus contributing to public health.
Subject(s)
Animals , Dogs , Seroepidemiologic Studies , Dogs/abnormalities , Serogroup , Bacterial Zoonoses/epidemiology , Leptospira , Leptospirosis/veterinaryABSTRACT
Objective: To analyze the incidence of leptospirosis in Fujian province from 2015 to 2020 and provide the scientific evidences for the risk assessment, prevention and control of leptospirosis. Methods: The incidence data of leptospirosis in Fujian during 2015-2020 were collected from China Information System for Disease Control and Prevention for a descriptive analysis, and software ArcGIS 10.3.1 was used for spatial autocorrelation analysis, and rats were captured in 17 surveillance areas during the same period, and the rat organs were collected for pathogen culture, the level of Leptospira antibody was detected in serum samples of rats, healthy population and the serum samples of patients sent by the hospitals. The infection status of Leptospira in human and rats were analyzed. Results: The incidence of leptospirosis in Fujian showed a downward trend from 2015 to 2020. A total of 176 cases of leptospirosis were reported. There were obvious seasonality and bimodal distribution. The majority of cases were farmers, accounting for 49.43% (87/176). Most cases were aged 30-69 years (85.80%, 151/176). The male to female ratio of the cases was 3.51∶1 (137∶39). Spatial autocorrelation analysis showed that leptospirosis had high or low clustering areas. From 2015 to 2020, the average capture rate of rats in 17 surveillance areas was 6.96% (1 519/21 838), Rattus losea, Rattus flavipectus and Niviventer fulvescens were the main species. The average positive rate of Leptospira antibody in rats was 28.64% (252/880). Java and Autumnalis were the predominant serogroups, accounting for 56.75% (143/252) and 17.46% (44/252), respectively. The average positive rate of Leptospira antibody in healthy population was 16.13% (254/1 575), and Autumnalis and Australis were the predominant serogroups, accounting for 71.65% (182/254). The confirmation rate of leptospirosis in patient serum samples sent by the hospitals was 2.23% (188/8 431), Autumnalis (56.38%, 106/188) and Hebdomadis (19.68%, 37/188) were the major serogroups. Conclusions: The incidence of leptospirosis in Fujian showed a downward trend from 2015 to 2020, there were obvious area clustering and seasonality. The high clustering areas were mainly distributed in northern, western and central Fujian. Java and Autumnalis were the predominant serogroups in rats. The infection rate in healthy population decreased year by year. Autumnalis and Hebdomadis were the main serogroups in population in Fujian.
Subject(s)
Animals , Female , Humans , Male , Rats , Antibodies, Bacterial , Incidence , Leptospira , Leptospirosis/epidemiology , SerogroupABSTRACT
Leptospira spp. constitui um grupo de bactérias espiroquetas gram-negativas englobando espécies saprofíticas, intermediárias e patogênicas, sendo as últimas agentes causadores da leptospirose, doença zoonótica de alcance mundial e endêmica em regiões tropicais em desenvolvimento. O crescente número de espécies identificadas de leptospiras destaca ainda mais sua diversidade genética e mecanismos de virulência únicos, muitos deles com função ainda desconhecida. Esforços para o desenvolvimento de novas vacinas com proteção cruzada e efeito duradouro revelaram possíveis candidatos vacinais que necessitam ser adequadamente validados, sendo assim, há ainda uma urgente necessidade de uma vacina universal contra a leptospirose capaz de controlar e reduzir os surtos cada vez mais frequentes da doença. Adesinas são importantes fatores de virulência em diversos patógenos, constituindo antígenos promissores para o desenvolvimento de vacinas contra a leptospirose, assim como para o desenvolvimento de métodos diagnósticos mais rápidos e precisos. Previamente, foram identificadas três proteínas hipotéticas conservadas em L. interrogans pela técnica de phage display, denominadas arbitrariamente como LepA069, LepA962 e LepA388. A expressão do gene codificador da proteína LepA069 apresentou aumento de aproximadamente 70 % em animais infectados por leptospiras virulentas, representando a primeira evidência funcional desta proteína ainda desconhecida. Porções recombinantes da lipoproteína hipotética LepA962 (LepA962_Nt e LepA962_Phg) foram obtidos, sendo demonstrada a forte interação da proteína LepA962_Phg, contendo a sequência identificada por phage display, com laminina, fibronectina plasmática, colágeno I e fibrinogênio de maneira dose-dependente. Adicionalmente, LepA962_Phg apresentou ligação às células VERO e à sua matriz extracelular secretada, e o soro obtido a partir desta proteína recombinante foi capaz de se ligar à superfície de leptospiras virulentas, indicando que LepA962_Phg pode representar um importante domínio de interação entre as leptospiras e seu hospedeiro. Finalmente, a proteína LepA388 pertencente a uma extensa família de proteínas modificadoras de virulência com função desconhecida (DUF_61), presente apenas nas leptospiras patogênicas mais virulentas, apresentou aumento na expressão de seu gene codificador em animais infectados por leptospiras virulentas de acordo com dados na literatura. Além disso, porções recombinantes da região Nterminal desta proteína apresentaram ligação a laminina, colágenos I e IV, vitronectina e fibronectinas plasmática e celular, principalmente considerando a sequência identificada por phage display. Estes dados reforçam as predições de modelos tridimensionais da proteína LepA388 e de outros membros da família DUF_61, as quais identificam domínios semelhantes a toxinas (como abrina e CARDS) responsáveis pela ligação e internalização celulares nos hospedeiros. Dados recentes sugerem um possível papel citotóxico desempenhado pelas proteínas desta família em leptospiras, as quais podem também ser consideradas potenciais candidatas vacinais e para diagnóstico da leptospirose, devido à sua distribuição restrita em espécies e cepas patogênicas de importância para saúde humana.
Leptospira spp. constitutes a group of gram-negative spirochete bacteria comprising saprophytic, intermediate and pathogenic species, the last being causative agents of leptospirosis, a zoonotic disease of worldwide extent and endemic in developing tropical regions. The growing number of identified leptospiral species further highlights their genetic diversity and unique virulence mechanisms, many of them with unknown function. Efforts to develop new vaccines with cross-protection and long-lasting effect have revealed possible vaccine candidates that need to be properly validated. Therefore, there is still an urgent need for a universal vaccine against leptospirosis capable of controlling and reducing the increasing outbreaks of the disease. Adhesins are important virulence factors in several pathogens, constituting promising antigens for the development of vaccines against leptospirosis, as well as for the development of faster and more accurate diagnostic methods. Previously, three conserved hypothetical proteins in L. interrogans were identified by phage display technique, arbitrarily named as LepA069, LepA962 and LepA388. Expression of the LepA069 encoding gene showed an increase of approximately 70 % in animals infected by virulent leptospires, representing the first functional evidence of this still unknown protein. Recombinant portions of the hypothetical lipoprotein LepA962 (LepA962_Nt and LepA962_Phg) were obtained, demonstrating the strong interaction of the LepA962_Phg protein, containing the sequence identified by phage display, with laminin, plasma fibronectin, collagen I and fibrinogen in a dose-dependent manner. Furthermore, LepA962_Phg showed binding to VERO cells and its secreted extracellular matrix, and the serum obtained from this recombinant protein was able to bind to the surface of virulent leptospires, indicating that LepA962_Phg may represent an important domain of interaction between leptospires and its host. Finally, LepA388 protein belonging to an extensive family of virulence modifying proteins with unknown function (DUF_61), present only in the most virulent pathogenic leptospires, showed an increase in the expression of its encoding gene in animals infected by virulent leptospires according to data in literature. Moreover, recombinant portions of the N-terminal region of this protein showed binding to laminin, collagens I and IV, vitronectin and plasma and cell fibronectins, especially considering the sequence identified by phage display. These data support the predictions of three-dimensional models of the LepA388 protein and other members of the DUF_61 family, which identify toxin-like domains (such as abrin and CARDS) responsible for cellular binding and internalization in hosts. Recent data suggest a possible cytotoxic role played by proteins of this family in leptospires, which can also be considered potential vaccine candidates and antigens for diagnosis, due to their restricted distribution in pathogenic species and strains of importance to human health
Subject(s)
Adhesins, Bacterial/classification , Virulence Factors/adverse effects , Vaccine Development/instrumentation , Leptospira interrogans/metabolism , Virulence , Vaccines/analysis , Dosage , Cell Surface Display Techniques , Leptospirosis/pathologyABSTRACT
The metropolitan region of Belém-PA suffers human fecal pollution in the local water supply system favoring the increase in cases of water circulating diseases in the population, especially the most socioeconomically vulnerable. Therefore, it is necessary to compile information on cases of these diseases over the years in this region, in order to better define public health policies. Thus, this work aims to contribute to the gathering of this information through a literature review selecting articles found in the SciELO, Pubmed and Google Academic databases with publications between 2005 and 2021 in Portuguese and English. The neighborhoods most affected by water circulation diseases proved were Guamá, Marco, Jurunas, Tapanã, Telegrafo and Terra Firme, located close to hydrographic basins, rivers and canals and are subject to flooding due to high tides and overflowing canals. In addition, the population in these neighborhoods is considered underprivileged. The most cited diseases and symptoms were leptospirosis, worms and diarrhea, which are easily prevented. Total and thermotolerant coliforms were also widely described, being the main indicators of fecal water contamination. The importance of sanitary measures is evidenced, as these reduce cases of water supply diseases in urban centers and improve population health. The quality of the water in the supply network is also important to mitigate the prevalence of these diseases.
Subject(s)
Humans , Water Pollution , Water Supply , Sanitation , Coliforms , Population Health , Health Policy , LeptospirosisABSTRACT
La leptospirosis es una enfermedad clasificada como zoonótica, y también es una de las más olvidadas. Como enfermedad endémica zoonótica, permanece en los lugares menos favorecidos afectando la salud humana y tambien de los ciertos animales domésticos. Tal afectación ocasiona pérdidas sustanciales económicas y sanitarias en las poblaciones vulnerables. La incidencia, se estima que afecta a un millón de seres humanos ocasionando la muerte a casi 59.000 de ellas. La transmisión de la leptospirosis humana es debida a la exposición directa o indirecta de las fuentes de infección primaria como animales infectados (orina o tejidos), y también por el contacto con alimentos o aguas contaminadas. En este trabajo se estudió la prevalencia y los factores de riesgos de leptospirosos en la industria porcícola en diferentes regiones del Estado Peruano. Los resultados mostraron que los trabajadores, en su mayoría, masculinos, con edades comprendidas entre 20 y 50 años fueron los más vulnerables a estos serovares de Leptospira, lo cual se relaciona con el contacto directo con porcinos y sus derivados. Por otra parte, las condiciones de vida de los trabajadores influyen en la mayor prevalencia de este servar. Condiciones rurales, falta de higiene, el contacto con mascotas sin tratamiento son factores de riego para la propagación de la leptospirosis(AU)
Leptospirosis is a disease classified as zoonotic, and it is also one of the most neglected. As an endemic zoonotic disease, it remains in the least favored places, affecting human health and also that of certain domestic animals. Such damage causes substantial economic and health losses in vulnerable populations. The incidence is estimated to affect one million human beings, causing the death of almost 59,000 of them. Transmission of human leptospirosis is due to direct or indirect exposure to primary infection sources such as infected animals (urine or tissues), and also by contact with contaminated food or water. In this work, the prevalence and risk factors of leptospirosis in the pig industry in different regions of the Peruvian State were studied. The results showed that workers, mostly male, aged between 20 and 50 years were the most vulnerable to these Leptospira serovars, which is related to direct contact with pigs and their derivatives. On the other hand, the living conditions of the workers influence the higher prevalence of this servar. Rural conditions, lack of hygiene, contact with untreated pets are risk factors for the spread of leptospirosis(AU)
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Zoonoses , Risk Factors , Vulnerable Populations , Industry , Animals, Domestic , Endemic Diseases , Pets , Infections , Leptospirosis/complicationsABSTRACT
RESUMEN La insuficiencia renal aguda es definida como la pérdida de función del riñón ocasionada por diversas causas, entre ellas infección e ingesta de fármacos. Esta entidad tiene alta morbilidad y mortalidad en las unidades de cuidados críticos. El tratamiento de la misma va desde la propia protección renal hasta la sustitución artificial de las funciones del riñón lesionado. En la actualidad la terapia de reemplazo renal continua se ha utilizado como soporte renal, y ofrece mayor estabilidad clínica a los pacientes más inestables. En esta revisión se comentan conceptos, indicaciones y los más recientes estudios que validan el uso de esta terapéutica, así como el método de programación que se utilizó en un paciente con diagnóstico de una leptospirosis icterohemorrágica (síndrome de Weil), que estuvo en shock séptico con disfunción multiorgánica, donde se empleó esta terapia con resultados satisfactorios (AU).
ABSTRACT Acute kidney failure is defined as the loss of kidney function caused by various causes, including infection and drug intake. This entity has high morbidity and mortality in critical care units. Treatment ranges from renal protection to artificial replacement of the functions of the injured kidney. Currently, continuous renal replacement therapy has been used as renal support, and offers greater clinical stability to the most unstable patients. In this review, authors discuss concepts, indications and the most recent studies that validate the use of this therapeutic, as well as the programming method that was used in a patient with diagnosis of icteric-hemorrhagic leptospirosis (Weil syndrome), who was in septic shock with multiorgan dysfunction, where this therapy was used with satisfactory results (AU).
Subject(s)
Humans , Male , Renal Replacement Therapy/methods , Leptospirosis/complications , Patients , Therapeutics/methods , Radiography, Thoracic/methods , Intensive Care UnitsABSTRACT
O objetivo do trabalho foi relatar a ocorrência de casos de cães com Leptospira spp. no Hospital Veterinário de Londrina. Foram avaliados 5.454 prontuários, durante os meses de janeiro a dezembro de 2016, e selecionados os que apresentaram solicitação de exame para Lepstopira por meio da técnica de campo escuro (CE). Nos positivos pela técnica CE dados sobre o sexo, sinais clínicos, endereço, data do atendimento e resultado na SAM foram coletados. A análise dos dados foi realizada pelo programa EpiInfo (7.2.3.1), dos 5.454 prontuários nos quais foi solicitado exame CE em 7,06% (385/5.454), e nesses, 13,76% (53/385) foi identificada a presença da espiroqueta. Dos animais positivos na técnica CE, 20,75% (11/53) foram reagentes na SAM. O sorovar Canicola foi o mais frequente em 90,91% (10/11) dos animais, apresentando títulos variados de 100 a 12.800. Para o sorovar Pomona 9,09% (1/11) dos animais apresentaram títulação de 100. Os sinais clínicos mais observados foram apatia em 58,49% (31/53), azotemia em 49,06% (26/53) e êmese em 49,06% (26/53) dos casos. Quanto ao sexo, os machos constituíram 71,70% dos casos e as fêmeas 28,30% (p=0,0015). Os resultados demonstraram a presença de Leptospira spp. entre os animais atendidos no hospital veterinário de Universidade Estadual de Londrina e reforçam a importância do sorovar Canicola entre os cães, visto que esse foi o mais frequente entre os sorovares e que apresentou os maiores títulos.(AU)
The purpose of this work is to report the occurrence of cases of dogs with Leptospira spp. at the Veterinary Hospital of Londrina. A total of 5,454 medical records were evaluated from January to December 2016, and data obtained from the examination for Lepstopira using the dark field technique (DF) were selected. In cases which were positive by the DF technique, data on gender, clinical signs, address, care data, and SAM results were collected. Data analysis was performed using the EpiInfo program (7.2.3.1). From the total 5,454 medical records in which DF examination was requested, in 7.06% (385/5.454), and from those, 13.76% (53/385) were positive for Leptospira. From the animals positive in the DF technique, 20.75% (11/53) were reactive in SAM. The Canicola serovar was the most prevalent, being present in 90.91% (10/11) of the animals, with titers ranging from 100 to 12,800. For the Pomona serovar, 9.09% (1/11) of the animals presented a title of 100. The most frequent clinical signs observed were apathy, in 58.49% (31/53); azotemia in 49.06% (26/53); and emesis in 49.06% (26/53) of the cases. As for gender, male animals constituted 71.70% of the cases and female 28.30% (p = 0.0015). The results demonstrated the presence of Leptospira spp. among animals treated at the Veterinary Hospital of the State University of Londrina which reinforces the importance of the Canicola serovar among dogs, since it was the serovar with the highest prevalence and highest titer found.(AU)
El objetivo de este trabajo fue reportar la ocurrencia de casos de perros con Leptospira spp. en el Hospital Veterinario de Londrina. Se evaluaron un total de 5.454 historias clínicas de enero a diciembre de 2016, y se seleccionó los que presentaron solicitud de examen de Lepstopira mediante la técnica de campo oscuro (CE). En los positivos por la técnica de CE se recogieron datos de sexo, signos clínicos, domicilio, fecha de atención y resultado en la SAM. El análisis de los datos se realizó mediante el programa EpiInfo (7.2.3.1), de las 5.454 historias clínicas en las que se solicitó examen CE en el 7,06% (385/5.454), y en estas, el 13,76% (53/385) se identificaron la de espiroqueta. De los animales positivos en la técnica CE, el 20,75% (11/53) fueron reactivos en la SAM. El serovar Canicola fue el más frecuente en el 90,91% (10/11) de los animales, con títulos que oscilaron entre 100 y 12.800. Para el serovar Pomona, el 9.09% (1/11) de los animales tuvo un título de 100. Los signos clínicos más observados fueron apatía en 58.49% (31/53), azotemia en 49.06% (26/53) y emesis en 49.06% (26/53) de los casos. Cuanto al sexo, los machos constituyeron el 71,70% de los casos y las hembras el 28,30% (p = 0,0015). Los resultados demostraron la presencia de Leptospira spp. entre los animales tratados en el Hospital Veterinario de la Universidad Estatal de Londrina y refuerzan la importancia del serovar Canicola entre los perros, ya que este fue el más frecuente entre los serovares y tuvo los títulos más altos.(AU)