Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
1.
Article in Chinese | WPRIM | ID: wpr-879550

ABSTRACT

OBJECTIVE@#To explore the genetic basis for a Chinese pedigree with a novel ABO subtype.@*METHODS@#The proband and his family members were subjected to serological analysis, and their genotypes were determined by fluorescence PCR and direct sequencing of the coding regions of the ABO gene. Exons 6 to 7 of the ABO gene were also subjected to clone sequencing for haplotype analysis.@*RESULTS@#The proband was determined as an AxB subtype. By fluorescence PCR, he was typed as A/B. Clone sequencing has revealed a insertional mutation c.797_798 insT in exon 7 of the ABO gene, which yielded a novel allele. Pedigree analysis confirmed that the novel ABO*A1.02 allele carried by the proband and his sister was inherited from their father. The c.797_798insT mutation has been submitted to GenBank with an accession number of MK125137.@*CONCLUSION@#The c.797_798insT mutation of exon 7 of the ABO gene probably has led to weakened expression of A antigen.


Subject(s)
ABO Blood-Group System/genetics , Alleles , China , Genotype , Humans , Male , Mutation , N-Acetylgalactosaminyltransferases/genetics , Pedigree
2.
Braz. j. med. biol. res ; 53(5): e9021, 2020. graf
Article in English | LILACS | ID: biblio-1098108

ABSTRACT

Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony formation. Wound healing and transwell invasion assays revealed that CHPF could positively regulate the migration of lung cancer cells. The tumorigenic role of CHPF was also validated in nude mouse xenograft models. Affymetrix gene chip analysis indicated that CHPF regulated the proliferation and invasion of lung cancer cells through CDH1, RRM2, MKI67, and TNFRSF10B. We thus highlight CHPF as a novel target for lung cancer treatment.


Subject(s)
Humans , Animals , Female , Rabbits , Gene Expression Regulation, Neoplastic , N-Acetylgalactosaminyltransferases/metabolism , Lung Neoplasms/metabolism , Blotting, Western , N-Acetylgalactosaminyltransferases/genetics , Cell Line, Tumor , Microarray Analysis , Cell Proliferation , Real-Time Polymerase Chain Reaction , Lung Neoplasms/genetics , Mice, Inbred BALB C
SELECTION OF CITATIONS
SEARCH DETAIL