Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 372
Filter
1.
Rev. ADM ; 80(5): 292-297, sept.-oct. 2023. ilus
Article in Spanish | LILACS | ID: biblio-1532061

ABSTRACT

La resorción ósea alveolar suele dar lugar a que las inserciones de la mucosa interfieran para la construcción, estabilidad y retención de una prótesis removible, una opción que permite modificar este tejido se obtiene por medio de una vestibuloplastia. Actualmente se puede favorecer la cicatrización de heridas utilizando láser de alta potencia aplicado a procedimientos quirúrgicos orales. Se realiza reporte de caso en paciente femenino a la que se realizó procedimiento de vestibuloplastia con láser de Er,Cr:YSGG, utilizando de forma postoperatoria gel de quitosano en nanotransportador biomolécula EPX. Se observa una cicatrización rápida y favorable al combinar ambas terapéuticas, además al utilizar productos con quitosano se disminuye el riesgo de la necrosis de fibroblastos gingivales humanos como recientemente se reportó en el uso de colutorios de clorhexidina (AU)


Alveolar bone resorption often results in mucosal insertions interfering with the construction, stability and retention of a removable prosthesis, an option to modify this tissue is obtained by means of vestibuloplasty. Currently, wound healing can be promoted by using high power laser applied to oral surgical procedures. A case report of a female patient who underwent a vestibuloplasty procedure with laser Er,Cr:YSGG, using chitosan gel with EPX biomolecule nanocarriers postoperatively. A fast and favorable healing is observed when combining both therapeutics, besides, when using products with chitosan, the risk of necrosis of human gingival fibroblasts is reduced, as recently reported in the use of chlorhexidine mouthwashes (AU)


Subject(s)
Humans , Female , Middle Aged , Wound Healing , Nanotechnology/methods , Laser Therapy/methods , Lasers, Solid-State , Chitosan
2.
Arq. ciências saúde UNIPAR ; 27(3): 1242-1268, 2023.
Article in Portuguese | LILACS | ID: biblio-1425458

ABSTRACT

Considerado um grave problema em saúde pública, as feridas crônicas são patologias que desafiam o manejo terapêutico e infelizmente acometem milhares de pessoas em todo o mundo. Essa doença apresenta altos índices de morbidade impactando negativamente na qualidade de vida dos seus portadores, além de influenciar negativamente no domínio "bem-estar", principalmente quando associado aos fatores clínicos podendo estar relacionado há anos de tratamento sem cura da ferida. As feridas crônicas são caracterizadas por demora ou dificuldade nos processos de cicatrização e reparação ordenada da integridade anatômica e funcional da pele durante um período de no mínimo três meses. Porém, algumas lesões permanecem por anos e até décadas sem cicatrizar. Objetivo: O escopo dessa revisão é mostrar o limitado arsenal terapêutico bem como a dificuldade no manejo clínico e dessa forma proporcionar uma reflexão sobre sua fisiopatologia e a urgente necessidade de novas opções e condutas terapêuticas que possam auxiliar no tratamento desses pacientes. Metodologia: Trata-se de uma revisão integrativa da literatura sobre feridas crônicas, cujo critérios de inclusão foram artigos publicados no período de janeiro de 2005 a fevereiro de 2023. Conclusão: A problemática acerca dessa patologia é vasta, tratando de uma doença de difícil cura, com uma gama de fatores associados que dificultam a cura da lesão, estendendo essa doença a altos índices de morbidade. Novas condutas terapêuticas e novos fármacos, precisam ser desenvolvidos urgentemente. Destaca-se que o uso de probióticos e o emprego da nanotecnologia tem mostrado um grande potencial inovador no tratamento de pacientes portadores de feridas crônicas.


Considered a serious public health problem, chronic wounds are pathologies that defy therapeutic management and unfortunately affect thousands of people around the world. This disease has high morbidity rates, negatively impacting the quality of life of its patients, in addition to negatively influencing the "well-being" domain, especially when associated with clinical factors, which may be related to years of treatment without healing of the wound. Chronic wounds are characterized by delay or difficulty in healing processes and orderly repair of the anatomical and functional integrity of the skin over a period of at least three months. However, some injuries remain for years and even decades without healing. Objective: The scope of this review is to show the limited therapeutic arsenal as well as the difficulty in clinical management and thus provide a reflection on its pathophysiology and the urgent need for new options and therapeutic approaches that can help in the treatment of these patients. Methodology: This is an integrative review of the literature on chronic wounds, whose inclusion criteria were articles published from January 2005 to February 2023. Conclusion: The problem surrounding this pathology is vast, dealing with a difficult-to-cure disease, with a range of associated factors that make healing of the lesion difficult, extending this disease to high morbidity rates. New therapeutic approaches and new drugs need to be developed urgently. It is noteworthy that the use of probiotics and the use of nanotechnology have shown great innovative potential in the treatment of patients with chronic wounds.


Consideradas un grave problema de salud pública, las heridas crónicas son patologías que desafían el manejo terapéutico y que, lamentablemente, afectan a miles de personas en todo el mundo. Esta enfermedad presenta altas tasas de morbilidad, impactando negativamente en la calidad de vida de sus pacientes, además de influir negativamente en el dominio "bienestar", especialmente cuando se asocia a factores clínicos, que pueden estar relacionados con años de tratamiento sin curación de la herida. Las heridas crónicas se caracterizan por un retraso o dificultad en los procesos de cicatrización y reparación ordenada de la integridad anatómica y funcional de la piel durante un periodo de al menos tres meses. Sin embargo, algunas heridas permanecen durante años e incluso décadas sin cicatrizar. Objetivo: El alcance de esta revisión es mostrar el limitado arsenal terapéutico así como la dificultad en el manejo clínico y así aportar una reflexión sobre su fisiopatología y la urgente necesidad de nuevas opciones y enfoques terapéuticos que puedan ayudar en el tratamiento de estos pacientes. Metodología: Se trata de una revisión integradora de la literatura sobre heridas crónicas, cuyos criterios de inclusión fueron artículos publicados desde enero de 2005 hasta febrero de 2023. Conclusiones: La problemática que rodea a esta patología es amplia, tratándose de una enfermedad de difícil curación, con una serie de factores asociados que dificultan la cicatrización de la lesión, extendiendo esta enfermedad a altas tasas de morbilidad. Es urgente desarrollar nuevos enfoques terapéuticos y nuevos fármacos. Cabe destacar que el uso de probióticos y el empleo de nanotecnología han mostrado un gran potencial innovador en el tratamiento de pacientes con heridas crónicas.


Subject(s)
Patients , Wounds and Injuries/drug therapy , Homeopathic Therapeutic Approaches , Therapeutics/nursing , Wound Healing , Databases, Bibliographic , Probiotics/therapeutic use , Nanotechnology/instrumentation , Anti-Infective Agents/therapeutic use
3.
Braz. J. Pharm. Sci. (Online) ; 59: e21769, 2023. tab, graf
Article in English | LILACS | ID: biblio-1439514

ABSTRACT

Abstract Currently, mucosal vaccine administration has stood out as an easier and non-invasive application method. It can also be used to induce local and systemic immune responses. In the COVID-19 pandemic context, nasal and oral vaccines have been developed based on different technological platforms. This review addressed relevant aspects of mucosal vaccine administration, with emphasis on oral and nasal vaccinations, in addition to the importance of using nanotechnology-based delivery systems to enable these strategies.


Subject(s)
Vaccines/analysis , Vaccination/adverse effects , Nanotechnology/instrumentation , Immunity/immunology
4.
Article in Chinese | WPRIM | ID: wpr-970617

ABSTRACT

With Zang-Fu organs, meridians, Qi and blood, and body fluid as the physiological and pathological basis, traditional Chinese medicine(TCM) theory is guided by the holistic concept and characterized by syndrome differentiation. It has made significant contributions to human health maintenance and disease prevention. Modern TCM preparation is developed on the basis of inheriting and developing TCM preparations using modern science and technology under the guidance of TCM theory. At present, the incidence and mortality of common tumors are increasing. TCM has rich clinical experience in the treatment of tumors. However, in the current stage, some TCM preparations have a tendency to deviate from the guidance of TCM theory. With the modernization of TCM, it is worth considering how TCM theory guides modern TCM preparations. Taking tumor treatment as an example, this paper introduced the development of TCM nano-preparation under the influence of modern nanotechnology, summarized the research on the development of modern TCM nano-preparation from the aspects of TCM holistic concept, TCM treatment principles, and TCM theory application, and discussed the application prospect of TCM nano-preparation in overall therapy, drug pairing, carrier selection, and targeted substance selection under the guidance of TCM theory. This paper provides new references for further developing the combination of tradition and modernization of TCM nano-preparation.


Subject(s)
Humans , Medicine, Chinese Traditional , Drugs, Chinese Herbal/therapeutic use , Biological Products , Nanotechnology , Neoplasms/drug therapy
5.
Article in Chinese | WPRIM | ID: wpr-1008657

ABSTRACT

Rheumatoid arthritis(RA) is a widely prevalent autoimmune inflammatory disease that severely affects patients' quality of life. Currently, conventional formulations against RA have several limitations, such as nonspecificity, poor efficacy, large drug dosages, frequent administration, and systemic side effects. Nanotechnology-based drug delivery systems have emerged as a promising stra-tegy for the diagnosis and treatment of RA since nanotechnology can overcome the limitations of traditional treatments and simplify the complexity of the disease. These systems enable targeted delivery of anti-inflammatory drugs to the inflamed areas through active and passive targeting, achieving specificity to the joints, overcoming the need for increased dosage and administration frequency, and reducing associated adverse reactions. This article aimed to review nanocarrier-based drug delivery systems in the field of RA and elucidate how nanosystems can be utilized to deliver therapeutic drugs to inflamed joints for controlling RA progression. By discussing the current issues and challenges faced by nanodrug delivery systems and highlighting the urgent need for solutions, this article offers theoretical support for further research on nanotechnology-based co-delivery systems in the future.


Subject(s)
Humans , Quality of Life , Drug Delivery Systems , Arthritis, Rheumatoid/drug therapy , Autoimmune Diseases/drug therapy , Nanotechnology
6.
Braz. j. biol ; 82: e241025, 2022. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1285594

ABSTRACT

The evolution of beauty market and personal care is constant in Brazil as well in the rest of the world. Technological advances have brought up nanotechnology to the cosmetological field, employing active principles at atoms enveloped by vesicles, in order to take the active principle precisely to the target tissue to optimize the results achieved because of the considerable ease to cross skin barriers. Manufacturing of nanotechnology cosmetics is confronted with low absorption capacity. One of the many active principle found in cosmetic industry is caffeine, a pseudoalkaloid from the xanthine group used as a stimulant with the mechanism of the lipolytic action. This active is widely used in a esthetics and cosmetics field in treatments involving dysfunctions such as localized fat and fibroedema geloid. To work out perfectly, the principle active need to interact and create a set of factors that includes lipolysis intensification. The caffeine encapsulation in gel-based nanocosmetics has the purpose of taking this active up to the adipocyte, the target cell, for mentioned dysfunctions treatment. Thus, we aim to present a review of how has been, the use of caffeine in the production of cosmetics.


A evolução do mercado de beleza e cuidados pessoais é constante no Brasil e no resto do mundo. Os avanços tecnológicos trouxeram a nanotecnologia para o campo cosmetológico, empregando princípios ativos em átomos envolvidos por vesículas, a fim de levar o princípio ativo precisamente ao tecido alvo para otimizar os resultados alcançados devido à considerável facilidade de atravessar barreiras cutâneas. A fabricação de cosméticos nanotecnológicos é confrontada com baixa capacidade de absorção. Um dos muitos princípios ativos encontrados na indústria cosmética é a cafeína, um pseudoalocalóide do grupo xantina usado como estimulante no mecanismo da ação lipolítica. Este ativo é amplamente utilizado no campo da estética e dos cosméticos em tratamentos que envolvem disfunções, como gordura localizada e fibroedema gelóide. Para funcionar perfeitamente, o princípio ativo precisa interagir e criar um conjunto de fatores que inclui a intensificação da lipólise. O encapsulamento de cafeína em nanocosméticos à base de gel tem o objetivo de levar esse ativo até o adipócito, a célula alvo, para o tratamento de disfunções mencionado. Assim, objetivamos apresentar uma revisão de como tem sido o uso de cafeína na produção de cosméticos.


Subject(s)
Skin Absorption , Cosmetics , Brazil , Caffeine , Nanotechnology
7.
Braz. j. biol ; 82: 1-7, 2022. ilus, tab
Article in English | LILACS, VETINDEX | ID: biblio-1468515

ABSTRACT

The evolution of beauty market and personal care is constant in Brazil as well in the rest of the world. Technological advances have brought up nanotechnology to the cosmetological field, employing active principles at atoms enveloped by vesicles, in order to take the active principle precisely to the target tissue to optimize the results achieved because of the considerable ease to cross skin barriers. Manufacturing of nanotechnology cosmetics is confronted with low absorption capacity. One of the many active principle found in cosmetic industry is caffeine, a pseudoalkaloid from the xanthine group used as a stimulant with the mechanism of the lipolytic action. This active is widely used in a esthetics and cosmetics field in treatments involving dysfunctions such as localized fat and fibroedema geloid. To work out perfectly, the principle active need to interact and create a set of factors that includes lipolysis intensification. The caffeine encapsulation in gel-based nanocosmetics has the purpose of taking this active up to the adipocyte, the target cell, for mentioned dysfunctions treatment. Thus, we aim to present a review of how has been, the use of caffeine in the production of cosmetics.


A evolução do mercado de beleza e cuidados pessoais é constante no Brasil e no resto do mundo. Os avanços tecnológicos trouxeram a nanotecnologia para o campo cosmetológico, empregando princípios ativos em átomos envolvidos por vesículas, a fim de levar o princípio ativo precisamente ao tecido alvo para otimizar os resultados alcançados devido à considerável facilidade de atravessar barreiras cutâneas. A fabricação de cosméticos nanotecnológicos é confrontada com baixa capacidade de absorção. Um dos muitos princípios ativos encontrados na indústria cosmética é a cafeína, um pseudoalocalóide do grupo xantina usado como estimulante no mecanismo da ação lipolítica. Este ativo é amplamente utilizado no campo da estética e dos cosméticos em tratamentos que envolvem disfunções, como gordura localizada e fibroedema gelóide. Para funcionar perfeitamente, o princípio ativo precisa interagir e criar um conjunto de fatores que inclui a intensificação da lipólise. O encapsulamento de cafeína em nanocosméticos à base de gel tem o objetivo de levar esse ativo até o adipócito, a célula alvo, para o tratamento de disfunções mencionado. Assim, objetivamos apresentar uma revisão de como tem sido o uso de cafeína na produção de cosméticos.


Subject(s)
Caffeine/therapeutic use , Cosmetics/analysis , Nanostructures/therapeutic use , Nanotechnology
8.
Einstein (Säo Paulo) ; 20: eRB5898, 2022. graf
Article in English | LILACS | ID: biblio-1360394

ABSTRACT

ABSTRACT The delivery of nucleic acids to cells is considered a crucial step for the success of genetic modifications aimed at therapeutic purposes or production of genetically modified animals. In this context, nanotechnology is one of the most promising fields of science, with the potential to solve several existing problems. Nanostructures have desirable characteristics to be used as carriers, such as nanometric size, large surface area, cell internalization capacity, prolonged and controlled release, among others. Genetically modified animals can contribute to the production of biopharmaceuticals, through the expression of high-associated-value molecules. The production of these animals, also known as biofactories, further enhances Brazilian agribusiness, since it allows adding value to the final product, and favors the integration between the agricultural market and the pharmaceutical sector. However, there is a growing concern about the safety and possible harmful effects of nanostructures, since data on the safe use of these materials are still insufficient. The objective of this review was to address aspects of the use of nanostructures, mainly carbon nanotubes as nucleic acid carriers, aiming at the production of genetically modified animals, with the certainty that progress in this field of knowledge depends on more information on the mechanisms of interaction between nanostructures, cells and embryos, as well as on its toxicity.


Subject(s)
Animals , Nucleic Acids , Nanotubes, Carbon , Nanostructures/toxicity , Nanostructures/chemistry , Drug Delivery Systems , Nanotechnology
9.
Acta Paul. Enferm. (Online) ; 35: eAPE01287, 2022. tab
Article in Portuguese | LILACS, BDENF | ID: biblio-1402915

ABSTRACT

Resumo Objetivo Verificar o conhecimento dos estudantes de enfermagem sobre o uso da nanotecnologia na área da saúde e aumentar sua sensibilidade para o assunto nos próximos anos. Métodos Estudo descritivo realizado com 523 estudantes de graduação em enfermagem. Os dados foram coletados por meio de um questionário online entre janeiro e fevereiro de 2018. Resultados A média de idade dos alunos foi de 21,7 ± 3,9 anos; 61,6% eram do sexo feminino. 29,4% afirmaram que a nanotecnologia deve ser utilizada na medicina, e 38,0% afirmaram que ela deve ser utilizada especialmente no diagnóstico, tratamento de doenças e na produção de novos equipamentos médicos. O maior percentual (38,8%) afirmou que a vantagem da nanotecnologia é a economia de tempo, enquanto 39,1% apontaram como desvantagem os seus efeitos tóxicos. Os alunos também afirmaram que ela deve ser utilizada no diagnóstico e tratamento de osteoporose, doenças cardiovasculares, diabetes mellitus, cicatrização de feridas e na produção de novos materiais. Conclusão De acordo com os resultados do estudo, os alunos chegaram às informações sobre nanotecnologia com seus esforços. Para os alunos, a nanotecnologia deveria ser mais utilizada na medicina, especialmente no diagnóstico-tratamento de doenças e na produção de novos equipamentos médicos. Eles determinaram a economia de tempo e os efeitos tóxicos como a principal vantagem e desvantagem da nanotecnologia, respectivamente. Sugerimos a importância de incluir esta tecnologia em treinamentos em hospitais e no currículo das faculdades.


Resumen Objetivo El objetivo de este estudio fue verificar el conocimiento de los estudiantes de enfermería sobre el uso de la nanotecnología en el área de la salud y aumentar su sensibilidad para el asunto en los próximos años. Métodos Estudio descriptivo realizado con 523 estudiantes universitarios de enfermería. Los datos fueron recopilados por medio de un cuestionario realizado en línea entre enero y febrero de 2018. Resultados La edad promedio de los alumnos fue de 21,7 ± 3,9; el 61,6 % era del sexo femenino. El 29,4 % de los alumnos afirmó que la nanotecnología debe ser utilizada en la medicina, mientras que el 38,0 % afirmó que debe ser especialmente utilizada en el diagnóstico, en el tratamiento de enfermedades y en la producción de nuevos equipos médicos. El mayor porcentaje (38,8 %) afirmó que la ventaja de la nanotecnología es el ahorro de tiempo, mientras que el 39,1 % afirmó que sus efectos tóxicos son una desventaja. Los alumnos también afirmaron que se debe utilizar en el diagnóstico y tratamiento de la osteoporosis, enfermedades cardiovasculares, diabetes mellitus, cicatrización de heridas y en la producción de nuevos materiales. Conclusión De acuerdo con los resultados del estudio, los alumnos llegaron a la información sobre nanotecnología mediante sus esfuerzos. Los alumnos consideraban que la nanotecnología debería ser más utilizada en la medicina. Expresaron que se debe usar especialmente en el diagnóstico-tratamiento de enfermedades y en la producción de nuevos equipos médicos. Determinaron las principales ventajas y desventajas de la nanotecnología, como el ahorro de tiempo y los efectos tóxicos, respectivamente. Sugerimos que es importante incluir esta tecnología en capacitaciones en servicio en hospitales y en diseños curriculares de las facultades.


Abstract Objective To determine nursing students' awareness of nanotechnology usage in healthcare and to increase their sensitivity to the subject in the coming years. Methods This descriptive study was developed with 523 undergraduate nursing students. Data were collected using an online questionnaire between January and February 2018. Results The mean age of the students was 21.7 ± 3.9 years; 61.6% were female. According to 29.4% of students, nanotechnology should be used in medicine and for 38.0%, it should be especially used in the diagnosis-treatment of diseases and the production of new medical equipment. The highest percentage (38.8%) of students affirmed that time saving is the advantage of nanotechnology, while 39.1% mentioned its toxic effects as a disadvantage. Students also stated it should be used in the diagnosis and treatment of osteoporosis, cardiovascular diseases, diabetes mellitus, wound healing, and the production of new materials. Conclusion According to results of the study, students achieved the information about nanotechnology with their efforts. They thought nanotechnology should be used more in medicine, especially in the diagnosis-treatment of diseases and in the production of new medical equipment. They determined that time-saving and toxic effects are the top advantage and disadvantage of nanotechnology, respectively. We suggest the importance of including this technology in service training in hospitals and in curricula of faculties.


Subject(s)
Humans , Male , Female , Adolescent , Young Adult , Students, Nursing , Awareness , Knowledge , Nanotechnology , Delivery of Health Care , Surveys and Questionnaires
10.
Braz. J. Pharm. Sci. (Online) ; 58: e20234, 2022. tab, graf
Article in English | LILACS | ID: biblio-1403740

ABSTRACT

Abstract Pyrostegia venusta (Ker Gawl.) Miers, popularly known as "Cipó-de São-João", has been used in traditional medicine for its therapeutic properties. Nanotechnology is able to enhance the pharmacological activity of plant extracts. In this context, liposomes and polymeric nanoparticles containing P. venusta ethanolic extract were developed and then physico-chemically characterized to evaluate the mutagenic/antimutagenic effects of P. venusta. In addition, transaminases and serum creatinine were biochemically analyzed for liver and renal damage, respectively. The micronucleus test was performed with male Swiss mice treated orally for 15 consecutive days with free extracts and nanostructured with P. venusta, and then intraperitoneally with N-ethyl-N-nitrosurea (50 mg/kg) on the 15th day of treatment. Micronucleated polychromatic erythrocytes (MNPCE) were evaluated in bone marrow. There was a significant reduction in the frequency of MNPCE (LPEPV = 183% and NPEPV = 114%, p < 0.001), indicating antimutagenic potential of the nanostructured extracts with P. venusta. The groups treated with only nanostructured extract did not show an increase in MNPCE frequency, and biochemical analyzes showed no significant difference between treatments. The liposomes and polymeric nanoparticles containing Pyrostegia venusta ethanolic extract showed biological potential in preventing the first step of carcinogenesis under the experimental conditions


Subject(s)
Animals , Male , Mice , Plant Extracts/adverse effects , Antimutagenic Agents , Bignoniaceae/classification , Flavonoids/analysis , Creatinine/agonists , Nanotechnology/instrumentation , Carcinogenesis/pathology
12.
Arq. bras. oftalmol ; 84(3): 282-296, May-June 2021. tab, graf
Article in English | LILACS | ID: biblio-1248965

ABSTRACT

ABSTRACT This review is intended to describe the therapeutic approaches for corneal blindness, detailing the steps and elements involved in corneal wound healing. It also presents the limitations of the actual surgical and pharmacological strategies used to restore and maintain corneal transparency in terms of long-term survival and geographic coverage. In addition, we critically review the perspectives of anabolic agents, including vitamin A, hormones, growth factors, and novel promitotic and anti-inflammatory modulators, to assist corneal wound healing. We discuss the studies involving nanotechnology, gene therapy, and tissue reengineering as potential future strategies to work solely or in combination with corneal surgery to prevent or revert corneal blindness.(AU)


RESUMO O presente trabalho traz uma revisão das abordagens terapêuticas para a cegueira da córnea. O estudo detalha as etapas e os elementos envolvidos na cicatrização da córnea. Ele mostra as limitações das estratégias cirúrgicas e farmacológicas usadas para restaurar e manter a transparência da córnea em termos de sobrevida a longo prazo e alcance geográfico. As perspectivas dos agentes anabólicos, incluindo vitamina A, hormônios, fatores de crescimento e novos moduladores pró-mitóticos e anti-inflamatórios para auxiliar a cicatrização da ferida na córnea, são revisadas criticamente. Aqui, apresentamos estudos envolvendo nanotecnologia, terapia gênica e reengenharia de tecidos como possíveis estratégias futuras para atuar de maneira isolada ou combinada com a cirurgia da córnea para prevenir ou reverter a cegueira corneana.(AU)


Subject(s)
Humans , Blindness/prevention & control , Blindness/therapy , Corneal Injuries/prevention & control , Corneal Injuries/therapy , Stem Cells , Vitamin A/therapeutic use , Genetic Therapy/instrumentation , Nanotechnology/instrumentation , Intercellular Signaling Peptides and Proteins/therapeutic use , Hormones/therapeutic use , Anti-Inflammatory Agents/therapeutic use
13.
Braz. arch. biol. technol ; 64: e21190387, 2021. tab, graf
Article in English | LILACS | ID: biblio-1153290

ABSTRACT

HIGHLIGHTS Production of lipid nanoemulsions (<100 nm) of industrial interest with low energy demand. The antioxidant properties of babassu oil have been improved and the nanoemulsions are not cytotoxic. Babassu oil is a food and medicinal product. The nanoemulsion is strategic for the developed of new antioxidants phytotherapeutics.


Abstract Background: Babassu oil is an extract from a Brazilian native coconut (Orbignya phalerata Martius) and is used both as a food and a medicinal product. Methods: we produced two babassu oil nanoemulsions and evaluated them regarding their nanoscopic stability, antioxidant activity and cytotoxicity.The nanoemulsions were characterized by Dynamic Light Scattering, and their stability was investigated for 120 days. The antioxidant activity was assessed by Spectroscopy Electron Paramagnetic Resonance, and the cytotoxicity was assessed by a colorimetric method (MTT) with the NIH/3T3 cell lineage. Results: the results showed nanoemulsions with average hydrodynamic diameter lower than 100 nm (p(0.001).and a polydispersity index of less than 0.3 (p(0.001), indicating monodisperse systems and good stability at room temperature. The exposure of nanoemulsions at varying pH revealed that the isoelectric point was at 3.0, and the images obtained by Transmission Electron Microscopy showed spherical droplets with a size 27 nm. The antioxidant activity showed that the babassu nanoemulsions exposed to free radicals had a better response when compared to the oil free samples. The cell viability assays showed low toxicity of the formulation with viability over 92% (p(0.05). Conclusion: babassu oil nanoformulations showed low polydispersity and kinetic stability with effective antioxidant action. Therefore, they can be promising for application in the food industry or as antioxidant phytotherapeutics.


Subject(s)
Palm Oil/chemistry , Nanotechnology , Antioxidants , Plant Extracts/chemistry , Food Industry , Cytotoxins , Microscopy, Electron, Transmission , Emulsions , Nanocomposites
14.
Article in English, Portuguese | LILACS | ID: biblio-1358277

ABSTRACT

Introdução: A nanotecnologia consiste no controle e manipulação da matéria em nível atômico e molecular, permitindo o desenvolvimento de dispositivos, materiais e sistemas com propriedades diferentes daquelas observadas na escala micro ou macroscópica. Objetivo: Verificar evidências científicas sobre a efetividade dos medicamentos em pacientes com câncer pelo uso da nanotecnologia. Método: Trata-se de uma revisão integrativa da literatura. A busca de artigos foi realizada nas bases de dados MEDLINE (PubMed), LILACS, SciELO, Scopus, Web of Science e BVS. Não houve restrição de localização, período e idioma. Para a seleção dos estudos, foi utilizada a combinação baseada no Medical Subject Heading Terms (MeSH). Incluíram-se na pesquisa estudos que obtiveram pontuação ≥ a 6 pontos, segundo o protocolo para pontuação qualitativa. Resultados: Foram localizados 216 artigos, sendo totalizados 208 após verificação por duplicação. Em seguida, os títulos e resumos foram analisados, sendo excluídos 205 trabalhos fora do escopo da proposta da pesquisa. Admitiram- -se, para a análise final, três artigos do tipo estudo clínico. Os nanocomplexos apresentaram eficiência nas amostras apresentadas, sendo eficazes quando o objetivo consistia em retardar a progressão de determinados tipos de cânceres. Conclusão: Os medicamentos com nanopartículas utilizados nos estudos demonstraram boa adesão pelos pacientes, assim como se apresentaram eficazes no tratamento de cânceres específicos. Contudo, estudos adicionais são necessários para explorar a atividade desses medicamentos


Introduction: Nanotechnology consists of the control and manipulation of matter at atomic and molecular level, allowing the development of devices, materials, and systems with properties different from those observed in the micro or macroscopic scale. Objective: Verify scientific evidence about the effectiveness of drugs in patients with cancer utilizing nanotechnology. Method: Integrative review of the literature. The search for articles was carried out in the databases MEDLINE (PubMed), LILACS, SciELO, Scopus, Web of Science e BVS. There was no restriction of location, period, and language. For the selection of the studies, the combination based on the Medical Subject Heading Terms (MeSH) was used. Studies that scored ≥ 6 points according to the qualitative scoring protocol were included in the study. Results: 216 articles were found, and after the elimination of duplicates, 208 remained. The titles and abstracts were analyzed, and 205 papers were excluded because they were not within the scope of the proposed study. Three articles of the type clinical trial were admitted for the final analysis. The nano complexes showed efficiency in the samples presented, being effective when the objective was to delay the progression of certain types of cancers. Conclusion: Medications with nanoparticles used in the studies showed good adherence by the patients, in addition to effectiveness in the treatment of specific cancers. However, additional studies are needed to explore the activity of these drugs


Introducción: La nanotecnología consiste en el control y manipulación de la materia a nivel atómico y molecular, permitiendo el desarrollo de dispositivos, materiales y sistemas con propiedades diferentes a las observadas a escala micro o macroscópica. Objetivo: Verificar la evidencia científica sobre la efectividad de los medicamentos en pacientes con cáncer mediante el uso de nanotecnología. Método: Se trata de una revisión integrativa de la literatura. La búsqueda de artículos se realizó en las bases de datos MEDLINE (PubMed), LILACS, SciELO, Scopus, Web of Science e BVS. No hubo restricción de ubicación, período e idioma. Para la selección de estúdios, se utilizó la combinación basada en los Términos de Encabezamiento de Materia Médica (MeSH). Los estudios que puntuaron ≥ 6 puntos según el protocolo de puntuación cualitativa. Resultados: Se encontraron 216 artículos, con un total de 208 luego de la verificación por duplicación. Luego, se analizaron los títulos y resúmenes, y se excluyeron 205 artículos por no estar en el alcance de la propuesta de investigación. Se admitieron tres artículos del tipo estudio clínico para el análisis final. Los nano complejos mostraron eficiencia en las muestras presentadas, siendo efectivas cuando el objetivo era retrasar la progresión de ciertos tipos de cánceres. Conclusión: Los fármacos de nanopartículas utilizados en los estudios mostraron una buena adherencia por parte de los pacientes, además de ser eficaces en el tratamiento de cánceres específicos. Sin embargo, se necesitan estudios adicionales para explorar la actividad de estos fármacos


Subject(s)
Humans , Female , Nanotechnology , Nanotechnology/methods , Drug Utilization , Access to Essential Medicines and Health Technologies , Neoplasms
15.
Arq. odontol ; 57: 236-243, jan.-dez. 2021. ilus, tab
Article in Portuguese | LILACS, BBO | ID: biblio-1348373

ABSTRACT

Objetivo:Avaliar in vitro a atividade de Staphylococcus aureus e Candida albicans em bases de próteses convencionais à base de polimetilmetacrilato de metila com nanopartículas de prata incorporadas a sua composição. Métodos: Foi realizado um estudo experimental laboratorial com resinas acrílicas autopolimerizáveis comercialmente disponíveis, Vipi Flash/VIPI e JET/Clássico. Foram confeccionados 80 corpos de prova, divididos em 16 grupos (n = 5), referentes ao tipo de resina, tratamento (incorporação e imersão na solução de nanopartículas de prata) e microrganismo inoculado. As nanopartículas foram sintetizadas com ácido polimetacrílico, nitrato de prata e irradiadas com luz ultravioleta de baixa potência (~8W) por 6 horas, e as suas concentrações idealizadas pelo método de microdiluição em placas para determinação da concentração mínima inibitória frente aos microrganismos selecionados. Verificou-se ação bactericida e fungicida com concentração inicial de 25% e após fator de diluição 12,5%. Resultados: Houve dificuldade de incorporação das nanopartículas na resina acrílica, que pode decorrer da alteração da proporção 3:1 recomendada pelo fabricante ou pela redução ou inativação da ação da nanopartícula de prata pela interação com o polimetilmetacrilato. VIPI com inclusão de nanopartícula obteve menor aderência de biofilme de Candida albicans. Conclusão:A nanopartícula de prata mostrou-se eficaz na sua ação de controle de Candida albicans e Staphylococcus aureus no método de imersão, entretanto, a sua ação antimicrobiana foi comprometida após inclusão nas resinas acrílicas.


Aim:To perform an in vitro evaluation of the activity of Staphylococcus aureus and Candida albicansin conventional prosthesis bases, based on methyl polymethylmethacrylate with silver nanoparticles incorporated into the composition. Methods: An experimental laboratory study was carried out using commercially available self-curing acrylic resins, Vipi Flash/VIPI and JET/Clássico. Eighty specimens were manufactured and divided into 16 groups (n = 5), referent to the resin brand, treatment (incorporation and immersion in the silver nanoparticle solution), and inoculated microorganism. The nanoparticles were synthesized with polymethacrylic acid and silver nitrate, and were irradiated with a low power (~ 8W) ultraviolet light for 6 hours. Their concentrations were idealized by the method of microplate dilution to determine the minimum inhibitory concentration when compared to the selected microorganisms. Bactericidal and fungicidal activities were identified with an initial concentration of 25% and a subsequent dilution factor of 12.5%. Results:It was difficult to incorporate the AgNPs into the acrylic resin, which may well have resulted from the change from the 3:1 proportion recommended by the manufacturer or by reducing or inactivating the action of the silver nanoparticle by interaction with polymethylmethacrylate. VIPI with the inclusion of nanoparticles obtained a lesser Candida albicans biofilm adherence. Conclusion: Silver nanoparticles were effective in controlling Candida albicans and Staphylococcus aureus in the immersion method; however, the antimicrobial activity was compromised after inclusion in acrylic resins.


Subject(s)
Silver Nitrate/pharmacology , Dental Prosthesis/microbiology , Polymethyl Methacrylate/pharmacology , Nanotechnology/methods , Nanoparticles/chemistry , Biological Control Agents/pharmacology , Staphylococcus aureus/drug effects , Candida albicans/drug effects , Anti-Infective Agents/pharmacology , Antifungal Agents/pharmacology
16.
Article in English | WPRIM | ID: wpr-921384

ABSTRACT

Framework nucleic acid (FNA) is a set of DNA nanostructures characterized by the framework morphology. It can design rational DNA sequences and follow the principle of complementary base pairing to construct FNA. The recent discovery of FNA constructed by DNA nanotechnology has great application potential in the field of bone regene-ration. It plays a positive role in the osteogenic differentiation of stem cells, bone regeneration, vascular regeneration, neuromodulation, immune regulation, and drug delivery. Here, we reviewed the current study findings on FNA in the field of bone regeneration.


Subject(s)
Bone Regeneration , Nanostructures , Nanotechnology , Nucleic Acids , Osteogenesis , Tissue Engineering
18.
Rev. Nutr. (Online) ; 33: e190165, 2020. tab, graf
Article in English | LILACS, SES-SP | ID: biblio-1136682

ABSTRACT

ABSTRACT Polyunsaturated fatty acids oxidize easily due to their chemical structure, causing a reduction of their nutritional properties. Nanostructured systems may be an alternative to protect fatty acids against oxidation, improving solubility and stability. Consequently, nutritional value of food is maintained as well as the sensory characteristics (color, flavor, texture, and aroma) when fatty acids are added to food products. The present study is a narrative review to introduce the potential benefits of omega-3 unsaturated fatty acids nanoparticles incorporated in food products. The literature review includes publications in English and Portuguese issued between March 1985 and March 2019, in PubMed, ScienceDirect and Web of Science databases. Manual searches were conducted in the articles references lists of the articles included to identify other relevant studies. There were studies that evaluated the stability of fatty acids in food products such as bread, fruit juice, milk, yogurt, and meat. In this study, the most used nanostructured systems for the incorporation of fatty acids were the nanocapsules and the nanoliposomes. Currently, the nanostructured system demonstrates a potential to improve protection of polyunsaturated fatty acids against oxidization and thermal degradation. In this way, they maintain their functional properties and their bioavailability increases and therapeutic efficacy and sensory properties are improved. There are several methodologies being tested, which makes it difficult to identify the most efficient formulation to protect fatty acids. Nanostructured systems seem to be the best alternative to protect polyunsatured fatty acids from oxidization. The encapsulation efficiency, particle's size and type are relevant factors to be considered to evaluate oxidization. In conclusion, the review showed that currently it is impossible to determine the most efficient methodology. Besides, nanoformulations should follow international guidelines to present more standardized and therefore more efficient particles.


RESUMO Os ácidos graxos poli-insaturados são facilmente oxidados devido à sua estrutura química, o que acarreta a diminuição de suas propriedades nutricionais. Nesse contexto, o sistema nanoestruturado pode ser uma alternativa para protegê-los contra a oxidação, melhorando a solubilidade e estabilidade. Consequentemente, quando são adicionados a produtos alimentares, o valor nutricional é mantido, bem como as características sensoriais (cor, sabor, textura e aroma). O presente estudo é uma revisão narrativa para apresentar os potenciais benefícios das nanopartículas com ácidos graxos insaturados da classe ômega-3 incorporados a produtos alimentícios. A literatura consultada incluiu publicações em inglês e em português, considerando o período entre março de 1985 e março de 2019, utilizando as bases de dados PubMed, ScienceDirect e Web of Science. Foram realizadas buscas manuais nas referências dos artigos incluídos, a fim de identificar outros estudos relevantes. Foram encontradas pesquisas que avaliaram a estabilidade dos ácidos graxos em produtos alimentícios, como pães, suco de fruta, leite, iogurte e carne. Neste estudo, as nanoestruturas mais utilizadas para a incorporação dos ácidos graxos foram as nanocápsulas e os nanolipossomas. Atualmente, o sistema nanoestruturado demonstra um potencial para melhorar a proteção desses ácidos poli-insaturados contra a oxidação e degradação térmica. Dessa forma, eles mantêm suas propriedades funcionais, aumenta-se sua biodisponibilidade e há melhora da eficácia terapêutica e das propriedades sensoriais. Existem diversas metodologias sendo testadas, o que dificulta a identificação de qual seria a formulação mais eficiente nessa proteção, mas os sistemas nanoestruturados parecem ser a melhor alternativa para proteger os ácidos graxos insaturados da oxidação. A eficiência de encapsulação bem como o tamanho e o tipo de partícula são fatores importantes a considerar na avaliação da oxidação. Em conclusão, a revisão demonstrou que atualmente a metodologia mais eficiente não é possível de ser identificada. Ademais, as nanoformulações devem seguir diretrizes do protocolo internacional para apresentar partículas mais padronizadas e, assim, eficientes.


Subject(s)
Fatty Acids, Omega-3 , Nanotechnology , Functional Food , Fatty Acids, Unsaturated
19.
Braz. arch. biol. technol ; 63: e20200234, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132219

ABSTRACT

Abstract Curcumin (CUR) shows potential use for treating cancer. However, CUR has low solubility and reduced bioavailability, which limit its clinical effect. Therefore, the development of nanocarriers can overcome these problems and can ensure the desired pharmacological effect. In addition, it is mandatory to prove the quality, the efficacy, and the safety for a novel nanomedicine to be approved. In that sense, this paper aimed (a) to prepare CUR-loaded polyethylene glycol-poly(ε-caprolactone) nanocapsules; (b) to validate an analytical method by high performance liquid chromatography (HPLC) for quantifying CUR in these nanoformulations; (c) to evaluate the physicochemical stability of these formulations; and to investigate their cytotoxicity on NIH-3T3 mouse fibroblast cells. The HPLC method was specific to CUR in the loaded nanocapsules, linear (r = 0.9994) in a range of 10.0 to 90.0 µg.mL-1 with limits of detection and quantification of 0.160 and 0.480 µg.mL-1, respectively. Precision was demonstrated by a relative standard deviation lower than 5%. Suitable accuracy (102.37 ± 0.92%) was obtained. Values of pH, particle size, polydispersity index, and zeta potential presented no statistical difference (p > 0.05) for CUR-loaded nanoparticles. No cytotoxicity was observed against NIH-3T3 mouse embryo fibroblast cell line using both the tetrazolium salt and sulforhodamine B assays. In conclusion, a simple and inexpensive HPLC method was validated for the CUR quantification in the suspensions of nanocapsules. The obtained polymeric nanocapsules containing CUR showed suitable results for all the performed assays and can be further investigated as a feasible novel approach for cancer treatment.


Subject(s)
Animals , Mice , Curcumin/pharmacology , Embryonic Stem Cells/drug effects , Fibroblasts/drug effects , Chromatography, High Pressure Liquid , Toxicity Tests , Nanotechnology , NIH 3T3 Cells , Embryo, Mammalian/cytology , Nanocapsules
20.
Braz. arch. biol. technol ; 63: e20190184, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132268

ABSTRACT

Abstract Nanoparticles demonstrate an important role in the protection of bioactive compounds from external factors such as temperature, oxygen and light. In this study, poly-ε-caprolactone (PCL) nanoparticles entrapped β-carotene was produced using the nanoprecipitation method. Firstly, was evaluated the lipophilic surfactant effect and carrier agent of the active compound in the nanocapsules formulation. After choosing the most stable formulation, the nanocapsules production was optimized using β-carotene, caprylic/capric triglycerides (CCT) and soybean lecithin. Response surface methodology (RSM) was adopted to evaluate the influence of soy lecithin concentration, volume of CCT and β-carotene concentration in the particle size, zeta potential, polydispersity index (PDI), encapsulation efficiency and recovery. Formulations containing soy lecithin and CCT demonstrated better stability comparing to the other formulations tested. The nanoparticle formulations presented an optimized particle size below 200 nm, PDI lower than 0.1 and encapsulation efficiency above 95%. Based on the results obtained, the optimum conditions to prepare PCL nanocapsules were 0.2160 mg/mL of β-carotene, 232.42 μL of CCT and 2.59 mg/mL of soy lecithin, suggesting an applicability to promote controlled released of β-carotene in food system.


Subject(s)
Caproates , beta Carotene , Nanotechnology/methods , Nanocapsules , Lactones , Chemical Precipitation , Bioreactors , Process Optimization
SELECTION OF CITATIONS
SEARCH DETAIL