Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 265
Filter
1.
Article in Chinese | WPRIM | ID: wpr-878434

ABSTRACT

Bone invasion by oral cancer is a common clinical problem, which affects the choice of treatment and predicts a poor prognosis. Unfortunately, the molecular mechanism of this phenomenon has not been fully elucidated. Current studies have revealed that oral cancer cells modulate the formation and function of osteoclasts through the expression of a series of signal molecules. Many signal pathways are involved in this process, of which receptor activator of nuclear factor-κB ligand/receptor activator of nuclear factor-κB/osteoprotegerin signaling pathway attracted much attention. In this review, we introduce recent progress in molecular mechanisms of bone invasion by oral cancer.


Subject(s)
Bone Resorption , Bone and Bones , Humans , Mouth Neoplasms , Osteoclasts , Osteoprotegerin , RANK Ligand , Receptor Activator of Nuclear Factor-kappa B
2.
Article in Chinese | WPRIM | ID: wpr-878423

ABSTRACT

OBJECTIVES@#This study aimed to explore the changes in the expression of the characteristic transcription factor retinoid related orphan receptor γt (RORγt) and the cytokine interleukin-17 (IL-17) of T helper cell 17 (Th17) in the pressure side of the periodontal tissue of rats under different orthodontic forces. Their effects on the expression of osteoprotegerin (OPG) and the quantity of osteoclast (OC) were also explored. The role of Th17 cell in alveolar bone remodeling under different forces was preliminarily investigated.@*METHODS@#A total of 108 rats were chosen and randomly divided into three groups. Mesial forces of 0, 50, and 100 g were loaded on the maxillary first molar in the three groups. The rats were executed at 0, 1, 3, 5, 7, and 14 days. The expression of RORγt mRNA was quantified by real-time quantitative polymerase chain reaction. The expression of IL-17 protein was quantified by enzyme linked immunosorbent assay. The expression levels of RORγt and OPG proteins were quantified, and the quantity of OC was counted via immunohistochemistry.@*RESULTS@#The expression levels of RORγt and IL-17 and the quantity of OC increased first and then decreased in the 50 and 100 g groups, and the peak values of the two groups were on days 5 and 7, respectively. The expression levels in the 50 g group basically recovered to normal level on day 14, while that in the 100 g group remained at a high level. The expression levels in the 50 g group were higher than those in the 0 g group and lower than those in the 100 g group. The expression of OPG in the 50 g group decreased first, then increased, and finally decreased. It basically recovered to normal level on day 14. The expression of OPG in the 100 g group decreased first and then increased. It remained at a high level on day 14. The expression in the 50 g group was significantly higher than that in the 0 g group on day 7, while the expression in the 100 g group was significantly higher than that in the 0 g group on day 14.@*CONCLUSIONS@#RORγt, IL-17, and OPG were expressed regularly over time under different orthodontic forces, indicating that Th17 participated in the process of bone resorption on the pressure side of periodontal tissue by secreting IL-17.


Subject(s)
Animals , Bone Resorption , Cytokines , Interleukin-17 , Molar , Nuclear Receptor Subfamily 1, Group F, Member 3 , Osteoclasts , Osteoprotegerin , Rats , Th17 Cells , Tooth Movement Techniques
3.
São Paulo; s.n; 2021. 69 p. ilust, tabelas.
Thesis in Portuguese | LILACS, Inca | ID: biblio-1222633

ABSTRACT

O carcinoma epidermóide (CEC) é a neoplasia maligna mais frequente em cavidade oral, representando aproximadamente 90% de todos os cânceres da boca. Esta neoplasia tem origem no epitélio de revestimento bucal, e a depender do subtipo e da sua localização, pode apresentar tendência marcante de invasão do tecido ósseo adjacente. Existe uma interação entre osso e neoplasia, e atenção principal deve ser dada aos reguladores relacionados à osteoclastogênese. As moléculas RANK/RANKL/OPG são fundamentais neste processo, e juntamente com a IL-6, promovem formação e ativação de osteoclastos, com consequente promoção da invasão mandibular. Neste sentido, o objetivo do estudo foi avaliar as características clínicas, radiográficas e histopatológicas dos pacientes submetidos a mandibulectomia para tratamento do carcinoma epidermóide oral, e correlacionar/associar com a expressão gênica na neoplasia de marcadores reguladores da invasão óssea. Foi realizado um estudo retrospectivo de pacientes tratados com algum tipo de mandibulectomia no A.C. Camargo Cancer Center durante o período de1990 a 2015. Um total de 220 casos foi selecionado, e destes, foram extraídos 40 casos, posteriormente submetidos as etapas de análise molecular. A análise molecular foi realizada por PCR quantitativa em tempo real (qPCR), por meio da mensuração de mRNA para os genes estudados, calibrados pelo gene endógeno GAPDH e por um pool de controle composto por RNA não neoplásico. Dos 220 pacientes incluídos na pesquisa, 161 eram do sexo masculino, a idade média foi de 60 anos, e 53,6% eram etilistas e 66,8% tabagistas. A invasão óssea da mandíbula foi observada através do laudo anatomopatológico em 77 pacientes, e foi associada à presença de margens comprometidas/exíguas e a localização da lesão (gengiva inferior, rebordo e região retro-molar). Ao final do estudo, 127 pacientes foram a óbito, e a média de sobrevida foi de 96,92 meses. Parâmetros clínicos, dos exames de imagem e histopatológicos foram significantemente associados com a sobrevida global, principalmente - tamanho do tumor, infiltração perineural, invasão óssea (imagem e anatomopatológico). Na análise multivariada, apenas invasão óssea e infiltração perineural aumentaram risco de óbito nos pacientes. Na análise molecular de expressão gênica foi possível avaliar que das 36 amostras do gene RANK, 16 eram downreguladas. Para RANKL, das 37 amostras, 28 estavam super expressas. Das 35 do gene da IL-6, 19 amostras eram downreguladas. E por fim, das 37 amostras do gene da OPG, 16 tinham expressão normal. A avaliação de associação entre a expressão dos genes analisados demostrou que o gene da IL-6 está associado a presença de invasão óssea, e que existe uma associação significativa entre as expressões do RANK, RANKL, OPG e IL-6. Maior sobrevida dos pacientes foi associada significativamente a uma expressão mais baixa de IL-6 (108,5 meses). Conclui-se que a invasão mandibular interfere na sobrevida global dos pacientes, e que este mecanismo de invasão óssea está associado com a expressão gênica dos genes RANK, RANKL, OPG e IL-6. A IL-6 downregulada nas células malignas está associada com maior sobrevida


Oral Squamous Cell Carcinoma (OSCC) is the most frequent malignant neoplasm in the oral cavity, representing 90% of all oral cancers. The tumor usually arises within the epithelium lining of the oral cavity, and in cases of certain subtype or location may reveal a tendency for invasion of the surrounding bone tissue. There is a relation between tumor and bone, and more attention should be given to the events related to osteoclastogenesis. The molecules RANK/RANKL/OPG are crucial in this process, and together with IL-6, promotes the formation and activation of osteoclasts, with influence on the mandibular invasion process. In this way, the aim of this study was to evaluate the clinical, demographic, imaging, and histopathology data of patients diagnosed with OSCC treated by mandibulectomy. Also, analyze the gene expression condition of the genes RANK, RANKL, OPG, and IL-6 on the tumor. A retrospective study was conducted on patients treated with mandibulectomy at the A.C. Camargo Cancer Center, between 1990 and 2015. Two hundred and twenty cases were retrieved, and 40 of them were selected for gene expression analysis. The mRNA expressions of the selected genes were determined by real-time polymerase chain reaction, extracted from the tumor, and calibrated by endogenous GAPDH and a control pool of non-neoplastic RNA. Among 220 patients, 161 were male, with a mean age of 60 years. 53.6% were alcohol users and 66.8% tobacco users. Mandible bone invasion was reported on 77 histopathology reports and associated with compromised margins and the site of the lesion (gingiva, alveolar ridge, and retromolar region). One hundred and twenty-seven patients died of the disease, and the mean overall survival rate was 96.92 months. Overall survival was associated with clinical, imaging, and pathological parameters, especially, tumor size, perineural growth, and bone invasion (both imaging and pathologic). Multivariate analysis revealed higher risk of death only to bone invasion and perineural growth. Gene expression analysis revealed - 16 downregulation of 36 RANK samples evaluated; 28 overexpression of 37 RANKL samples evaluated; 19 downregulation of 35 IL-6 analyzed, and 16 normal expression of 37 OPG samples evaluated. Association analysis revealed that IL-6 expression is associated with bone invasion, and there is also a significant association among the expression of RANK, RANKL, OPG, and IL-6. Higher overall survival rate was associated with an IL-6 down expression (108;5 months). In conclusion, mandibular invasion interferes with the patient's overall survivall rate, and the bone invasion mechanism is associated with gene expression of RANK, RANKL, OPG, and IL-6 on the tumor. IL-6 in downregulation expressed by malignant cells is associated with higher overall survivor rates


Subject(s)
Humans , Female , Pregnancy , Adult , Middle Aged , Prognosis , Carcinoma, Squamous Cell , Interleukin-6 , Receptor Activator of Nuclear Factor-kappa B , Osteoprotegerin , Mandible
4.
Braz. dent. j ; 31(1): 63-68, Jan.-Feb. 2020. tab, graf
Article in English | LILACS | ID: biblio-1089266

ABSTRACT

Abstract The present study evaluated polymorphisms in RANK, RANKL and OPG-encoding genes to assess whether they are associated with mucositis and peri-implantitis in a population from the Brazilian Amazon region. One hundred and fourteen patients with dental implants were included in the study. After clinical and radiographic examination, the sample was categorized into 4 groups, according to the peri-implant status: Healthy (n=71), Mucositis (n=30), Peri-implantitis (n=13) and Diseased (Mucositis + Peri-implantitis, n=43). Genomic DNA was extracted from buccal cells from saliva, and the genetic polymorphism in osteoprotegerin (OPG), Kappa nuclear factor activator receptor (RANKL) and nuclear kappa factor activator receptor (RANK) were genotyped by the real time PCR. Univariate and multivariate statistical analyses were performed to compare clinical variables among groups and to evaluate genotypes and alleles distributions and the established alpha was 5%. Age, peri-implant biotype, diabetes and presence of peri-implant biofilm were associated with mucositis (p<0.05) and peri-implantitis (p<0.05). Smoking, alcoholism, and periodontal biofilms were also associated with the presence of peri-implantitis (p<0.05). Univariate and multivariate analysis did not demonstrate an association of peri-implantitis or mucositis with any genetic polymorphism in RANK (rs3826620), RANKL (rs9594738) and OPG (rs2073618) (p>0.05). The studied genetic polymorphism in RANK, RANKL and OPG were not associated with mucositis and peri-implantitis in a Brazilian population from the Amazon region.


Resumo O presente estudo avaliou a associação da predisposição clínica e dos fatores genéticos com a presença de doenças peri-implantares. Cento e quatorze pacientes com implantes dentais instalados na Clínica de Especialização do Amazonas, Brazil, foram incluidos no estudo. Após exame clínico e radiográfico, a amostra foi categorizada em 4 grupos, de acordo com o Status peri-implantar: saúde (n=71), mucosite (n=30), peri-implantite (n=13) e doentes (mucosite + peri-implantite). DNA genômico foi extraído de células orais da saliva, e o polimorfismo genético em osteoprotegerina (OPG), ligante do receptor ativador do fator Kappa nuclear (RANKL) e receptor ativador do fator Kappa nuclear (RANK) foram genotipados por PCR em tempo real. O estudo se propôs a avaliar se os polimorfismos em RANK, RANKL e OPG estão envolvidos na patogênese da mucosite e da peri-implantite, e avaliar também a presença de fatores de risco moduladores da resposta em uma população brasileira. Idade, biotipo peri-implantar, diabetes e presença de biofilme peri-implantar foram associados a mucosite (p<0.05) e peri-implantite (p<0.05). Tabagismo, alcoolismo e biofilme periodontal também foram associados com a presença de peri-implantite (p<0.05). Análise univariada e multivariada não demonstraram associação de peri-implantite ou mucosite com os polimorfismos genéticos em RANK (rs3826620), RANKL (rs9594738) e OPG (rs2073618) (p>0.05). Os polimorfismos genéticos estudados não foram associados com mucosite e peri-implantite em uma população brasileira da região Amazônica.


Subject(s)
Humans , Dental Implants , RANK Ligand/genetics , Receptor Activator of Nuclear Factor-kappa B/genetics , Osteoprotegerin/genetics , Peri-Implantitis , Polymorphism, Genetic , Brazil , Mouth Mucosa
5.
São Paulo; s.n; 20200000. 81 p.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1119580

ABSTRACT

Pacientes com cirrose hepática podem desenvolver osteoporose como complicação relacionada ao déficit funcional hepático. O objetivo desse estudo é o de avaliar a presença de proteínas relacionadas ao metabolismo ósseo (RANKL, OPG, IL-1?, IL-6 e TNF-?) na saliva e no sangue de pacientes cirróticos e compará-los com o índice da cortical mandibular (ICM), relacionado à densidade mineral óssea, em radiografias panorâmicas. Trinta e oito pacientes cirróticos foram submetidos à anamnese, exame físico e tiveram amostras de sangue e de saliva não estimulada coletadas. Desses, 22 apresentavam radiografias panorâmicas que foram avaliadas segundo o ICM. As proteínas foram avaliadas através da tecnologia LuminexTM xMAP. Foram considerados estatisticamente significativos valores de p <=0.05. A maioria dos pacientes era do sexo masculino (68,4%), com idade média de 50,61 anos, média de MELD de 18 pontos no momento da consulta e média de 21,34 meses na fila de espera para o transplante. A análise das radiografias panorâmicas permitiu constatar que a maior parte da amostra apresentava alguma modificação na cortical mandibular sugestiva de alterações osteoporóticas (72,7%). O perfil de expressão das proteínas foi bastante variável no sangue e na saliva. Correlação estatisticamente significativa foi observada entre a expressão salivar de RANKL e OPG em pacientes com ICM alterado. Não foi observada relação estatisticamente significante entre o perfil das proteínas e a etiologia da cirrose, tempo na fila de transplante hepático, idade e sexo dos pacientes. As proteínas RANKL, OPG, IL-1?, IL-6 e TNF-? se comportam de maneira diferente na saliva e no sangue. Valores elevados de RANKL e OPG em saliva estão relacionados com ICM sugestivo de osteoporose em pacientes cirróticos, e propõe-se que esse aumento ocorra devido ao microambiente inflamatório local.


Subject(s)
Osteoporosis , Radiography, Panoramic , RANK Ligand , Osteoprotegerin , Inflammation , Liver Cirrhosis
6.
J. appl. oral sci ; 28: e20190409, 2020. graf
Article in English | LILACS, BBO | ID: biblio-1090768

ABSTRACT

Abstract Menopause induces oral bone loss, leading to various oral diseases. Mastication importantly affects bone metabolism in the jawbone. Objective: To analyze the effect of enhanced masticatory force on osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), and mechano-growth factor (MGF) in alveolar bone of ovariectomized rats and to study the mechanics mechanism of the alveolar bone of ovariectomized rats response to enhanced masticatory force. Methodology: Thirty Sprague Dawley rats were randomly divided into three groups: sham-operation group (fat around the removed ovary + normal hard diet), model group (ovariectomy + normal hard diet), and experimental group (ovariectomy + high hard diet). It was a 2-month experiment. Enzyme-linked immunosorbent assay (ELISA) detected serum estradiol (E2), osteocalcin (BGP) and alkaline phosphatase (ALP) in rats. Bone histomorphometric indices in the third molar region of maxilla were detected by micro-CT; protein expressions of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Western blot; and gene expression of OPG, RANKL, and MGF in the third molar region of maxilla was detected by Quantitative Real-Time PCR. Results: Comparing with model group, serum E2 in experimental group increased but not significantly, serum BGP and serum ALP in experimental group decreased but not significantly, OPG in experimental group in alveolar bone increased significantly, RANKL in experimental group in alveolar bone decreased significantly, RANKL/OPG ratio in experimental group decreased significantly, MGF in experimental group in alveolar bone increased significantly, bone volume to total volume fraction increased significantly in experimental group, trabecular thickness increased significantly in experimental group, and trabecular separation decreased significantly in experimental group. Conclusion: Enhanced masticatory force affected the expression of OPG, RANKL, and MGF in alveolar bone of ovariectomized rats, improved the quality of jaw bone of ovariectomized rats, and delayed oral bone loss by ovariectomy.


Subject(s)
Animals , Female , Bite Force , Insulin-Like Growth Factor I/analysis , Ovariectomy , RANK Ligand/analysis , Osteoprotegerin/analysis , Alveolar Process/physiopathology , Osteocalcin/blood , Blotting, Western , Polymerase Chain Reaction , Rats, Sprague-Dawley , Alkaline Phosphatase/blood , Estradiol/blood , X-Ray Microtomography , Enzyme-Linked Immunospot Assay
7.
Braz. oral res. (Online) ; 34: e014, 2020. tab, graf
Article in English | LILACS | ID: biblio-1089383

ABSTRACT

Abstract Although dental implants and bone regenerative procedures are important approaches for the reestablishment of esthetics and function in young patients with a history of generalized aggressive periodontitis (GAP), no predictable outcomes have been reported, and the host osteo-immunoinflammatory response may play a relevant role in this context. In view of the lack of molecular investigations into the bone tissue condition of young patients with periodontitis, the aim of this study was to evaluate the gene expression of bone-related factors in this population. Bone biopsies were obtained from the posterior mandible in 16 individuals previously diagnosed with GAP and on periodontal support therapy and from 17 periodontally healthy (PH) patients. The gene expression of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), osteocalcin (OC), bone sialoprotein (BSP), and type I collagen (COL-I), important biomarkers of bone turnover, was evaluated by qRT-PCR. Lower TGF-β and OPG mRNA levels were observed in GAP patients compared to PH individuals (p ≤ 0.05). There were no between-group differences in levels of TNF-α, BSP, RANKL, OC, or COL-I mRNA (p>0.05). In young adults, a history of periodontal disease can negatively modulate the gene expression of important bone-related factors in alveolar bone tissue. These molecular outcomes may contribute to the future development of therapeutic approaches to benefit bone healing in young patients with history of periodontitis via modulation of osteo-immuno-inflammatory biomarkers.


Subject(s)
Humans , Male , Female , Adult , Young Adult , Aggressive Periodontitis/genetics , Gene Expression , Aggressive Periodontitis/metabolism , Reference Values , Biomarkers , Osteocalcin/analysis , Osteocalcin/genetics , Single-Blind Method , Cross-Sectional Studies , Transforming Growth Factor beta/analysis , Transforming Growth Factor beta/genetics , Tumor Necrosis Factor-alpha/analysis , Tumor Necrosis Factor-alpha/genetics , Statistics, Nonparametric , Collagen Type I/analysis , Collagen Type I/genetics , RANK Ligand/analysis , RANK Ligand/genetics , Osteoprotegerin/analysis , Osteoprotegerin/genetics , Integrin-Binding Sialoprotein/analysis , Integrin-Binding Sialoprotein/genetics , Alveolar Process/chemistry , Real-Time Polymerase Chain Reaction
8.
Article in English | LILACS, BBO | ID: biblio-1135564

ABSTRACT

Abstract Objective: To investigate the differences of receptor activator of nuclear factor-κB ligand (RANKL) and Osteoprotegerin (OPG) expressions between normoglycemic and hyperglycemic Wistar rats (Rattus Novergicus) during Orthodontic Tooth Movement (OTM). Material and Methods: This study was true experimental with post-test group only. Thirty-two healthy male Wistar rats, weighted around 200-250 grams, 12-20 weeks old, were used as OTM animal study. They were divided into 2 groups (n=16), normoglycemic rats (normal blood glucose 80-120 mg/dl) and hyperglycemic rats (>250 mg/dl) induced by Streptozotocin with a dose of 30 mg in PBS injection intraperitoneally. A NiTi closed coil spring was mounted between maxillary first molar and incisors with the light force 10gf/mm2 in both groups to induce OTM. The studied animals were then terminated on days 1, 3, 6, and 9, respectively, and premaxilla was extracted. RANKL and OPG expression were examined utilizing immunohistochemistry (IHC) analysis. One-way ANOVA and Tukey HSD (p<0.05) were utilized to analyze the differences in the expression of RANKL and OPG between groups. Results: The hyperglycemic group on day 1, 9 rats showed a significant increase in the expression of RANKL, whereas OPG expression decreased significantly on days 1, 3, and 9. Conclusion: There was a significant increase of RANKL expression and a decrease of OPG expression in hyperglycemic rats as documented immunohistochemically.


Subject(s)
Animals , Rats , Tooth Movement Techniques , Rats, Wistar , Streptozocin , Diabetes Mellitus , RANK Ligand , Hyperglycemia , Immunohistochemistry , Epidemiology, Experimental , Analysis of Variance , Investigative Techniques , Osteoprotegerin , Molar
9.
Actual. osteol ; 15(3): 180-191, Sept-Dic. 2019. ilus
Article in English | LILACS | ID: biblio-1104226

ABSTRACT

Bone metabolism disorders are characterized by an imbalance of bone resorption and formation in the bone remodeling process. Glucocorticoids that are used to treat kidney diseases exacerbate these disorders. P-selectin and galectin-3 are molecules involved in the sclerotic process in kidney, whereas bone resorption is regulated by the interaction between the nuclear factor activator kappa b receptor (RANK), its ligand (RANKL) and the RANKL decoy receptor osteoprotegerin (OPG). The aim of this study was to investigate the cellular and molecular mechanisms of disruption of bone remodeling regulation processes, reflected by intercellular mediators (RANKL, OPG, P-selectin and galectin-3) in chronic kidney disease experimental model treated with glucocorticoids. Rats were divided into four groups of 10 animals each. The first group, the control group, included intact animals. The second group consisted of rats with impaired bone remodeling resulting from chronic kidney disease (experimental group (CKD). The third group was a group of animals with impaired bone remodeling due to exposure to glucocorticoids (experimental group (GCs)). The fourth group consisted of rats with impaired bone remodeling in chronic kidney disease, followed by exposure to glucocorticoids (experimental group (CKD + GCs)). The effects of CKD and glucocorticoid were evaluated biochemically, histologically and by measuring bone density. An enzymelinked immunoassay was used to measure intercellular mediator levels in the serum. The bone density in the experimental groups was reduced compared to the control group. RANKL levels in animals of three experimental groups were higher than in intact animals. Serum levels of OPG were higher in CKD and GCs groups than in intact animals. At the same time, in the animals' blood serum of the CKD + GCs group, the levels of OPG were lower, than those in animals from the control group. The levels of galectin-3 in the serum of the experimental groups GCs and CKD + GCs were lower than in intact animals. The serum levels of galectin-3 in animals of the CKD group were higher than those in animals from the control group. The levels of P-selectin were lower in the serum of the GCs group than in intact animals. At the same time, the levels of P-selectin were higher in the CKD and CKD + GCs groups, than those in animals from the control group. In conclusion, the study of the complex system of bone remodeling regulation, which includes many factors and their interactions, may lead to the development of new methods for treating patients with chronic kidney disease in order to prevent osteoporosis in the future. (AU)


Las enfermedades metabólicas óseas se caracterizan por un desequilibrio en el proceso de remodelación ósea en los que participan mediadores tales como receptor del activador del factor nuclear- kappa- b (RANK), su ligando (RANKL) y la osteoprotegerina (OPG). Los glucocorticoides, recuentemente empleados en el tratamiento de la enfermedad renal crónica, exacerban este desequilibrio. En la enfermedad esclerótica renal, las moléculas de adhesión celular P-selectina and galectina-3 tienen un rol fundamental. El objetivo de esta trabajo fue estudiar las alteraciones en los mediadores de la remodelación ósea (RANKL, OPG, P-selectina and galectina-3) en un modelo de enfermedad renal crónica con tratamiento glucocorticoideo. Ratas Wistar hembras fueron divididos en 4 grupos: control (C); enfermedad renal crónica con afección de la remodelación ósea (ERC); animales con afección de la remodelación ósea expuestos a glucocorticoides (GC); enfermedad renal crónica con afección de la remodelación ósea tratados con glucocorticoides (ERC+GC). Los efectos de la ERC y los GC fueron evaluados bioquímicamente, histológicamente y por medición de la densidad ósea. RANKL, OPG, Pselectina and galectina-3 se cuantificaron en muestras de sangre venosa empleando enzimoinmuno análisis. En los 3 grupos experimentales la densidad ósea se evidenció reducida y los niveles séricos de RANKL elevados respecto al grupo control. Los niveles de OPG en los grupos ERC y GC fueron superiores mientras que en el grupo ERC+GC menores respecto a los animales controles. Galectina 3 plasmática en GC y ERC+GC se encontró reducida y aumentada en los animales ERC, en comparación con los animales controles. La concentración sérica de P-selectina sérica fue mayor en los grupos ERC y ERC+GC, y menor en los animales GC respecto a los niveles plasmáticos de los animales intactos. El avance del conocimiento sobre la regulación de la remodelación ósea a través de la interacción de mediadores sistémicos, en un futuro, puede conducir al desarrollo de nuevas estrategias terapéuticas para la prevención de la osteoporosis en pacientes con enfermedad renal crónica. (AU)


Subject(s)
Animals , Rats , Chronic Kidney Disease-Mineral and Bone Disorder/chemically induced , Bone Remodeling/drug effects , Kidney Diseases/physiopathology , Osteoporosis/prevention & control , Bone Diseases, Metabolic/diagnosis , Dexamethasone/administration & dosage , Bone Density/drug effects , Chloroform/therapeutic use , Rats, Wistar , P-Selectin/drug effects , P-Selectin/blood , Galectin 3/drug effects , Galectin 3/blood , RANK Ligand/drug effects , RANK Ligand/blood , Osteoprotegerin/drug effects , Osteoprotegerin/blood , Glucocorticoids/adverse effects , Glycerol/administration & dosage , Kidney Diseases/drug therapy
10.
J. appl. oral sci ; 27: e20180476, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-1040231

ABSTRACT

Abstract Objectives: Miniscrew has been frequently used, considering that anchorage control is a critical point in orthodontic treatment, and its failure, the main adverse problem. Using two groups of stable (successful) and unstable (failed) mini-implants, this in vivo study aimed to quantify proinflammatory cytokines IL-1 α, IL-6, IL-17, and TNF-α and osteoclastogenesis marker RANK, RANKL, and OPG in gingival tissue, using the real-time polymerase chain reaction technique. Methodology: Thirteen patients of both sexes (11-49 years old) under orthodontic treatment were selected, obtaining 11 successful and 7 failed mini-implants. The mini-implants were placed and removed by the same surgeon, in both jaws. The mean time of permanence in the mouth was 29.4 months for successful and 7.6 months for failed mini-implants. At removal time, peri-mini-implant gingival tissue samples were collected and processed for quantification of the proinflammatory cytokines and osteoclastogenesis markers. Nonparametric Wilcoxon rank-sum test considering the clusters and Kruskal-Wallis test were used for statistical analysis (α=0.05). Results: No significant difference (p>0.05) was observed between the groups for either quantification of cytokines or osteoclastogenesis markers, except for IL-6 (p<0.05). Conclusions: It may be concluded that the expression of IL-1α, IL-17, TNF-α, RANK, RANKL, and OPG in peri-implant gingival tissue were not determinant for mini-implant stability loss, but the higher IL-6 expression could be associated with mini-implant failure.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Osteogenesis/physiology , Dental Implants/adverse effects , Cytokines/analysis , Orthodontic Anchorage Procedures/adverse effects , Peri-Implantitis/pathology , Gingivitis/pathology , Reference Values , Time Factors , Biomarkers/analysis , Alveolar Bone Loss , Treatment Outcome , Statistics, Nonparametric , Osteoprotegerin/analysis , Real-Time Polymerase Chain Reaction
11.
J. appl. oral sci ; 27: e20180671, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-1019970

ABSTRACT

Abstract Objective: To monitor early periodontal disease progression and to investigate clinical and molecular profile of inflamed sites by means of crevicular fluid and gingival biopsy analysis. Methodology: Eighty-one samples of twenty-seven periodontitis subjects and periodontally healthy individuals were collected for the study. Measurements of clinical parameters were recorded at day −15, baseline and 2 months after basic periodontal treatment aiming at monitoring early variations ofthe clinical attachment level. Saliva, crevicular fluid and gingival biopsies were harvested from clinically inflamed and non-inflamed sites from periodontal patients and from control sites of healthy patients for the assessment of IL-10, MMP-8, VEGF, RANKL, OPG and TGF-β1 protein and gene expression levels. Results: Baseline IL-10 protein levels from inflamed sites were higher in comparison to both non-inflamed and control sites (p<0.05). Higher expression of mRNA for IL-10, RANK-L, OPG, e TGF-β1 were also observed in inflamed sites at day −15 prior treatment (p<0.05). After the periodontal treatment and the resolution of inflammation, seventeen percent of evaluated sites still showed clinically detectable attachment loss without significant differences in the molecular profile. Conclusions: Clinical attachment loss is a negative event that may occur even after successful basic periodontal therapy, but it is small and limited to a small percentage of sites. Elevated inflammation markers of inflamed sites from disease patients reduced to the mean levels of those observed in healthy subjects after successful basic periodontal therapy. Significantly elevated both gene and protein levels of IL-10 in inflamed sites prior treatment confirms its modulatory role in the disease status.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Periodontal Attachment Loss/pathology , Periodontitis/therapy , Saliva/chemistry , Time Factors , Biopsy , Biomarkers/analysis , Case-Control Studies , Cytokines/analysis , Gingival Crevicular Fluid/chemistry , Statistics, Nonparametric , Matrix Metalloproteinase 8/analysis , Vascular Endothelial Growth Factor A/analysis , Osteoprotegerin/analysis , Real-Time Polymerase Chain Reaction , Gingiva/pathology
12.
Chinese Medical Journal ; (24): 25-29, 2019.
Article in English | WPRIM | ID: wpr-772844

ABSTRACT

BACKGROUND@#Obstructive sleep apnea-hypopnea syndrome (OSAHS) is associated with a higher prevalence of osteoporosis. However, the underlying mechanisms linking OSAHS with bone loss are still unclear. The aim of this study was to investigate the changes of receptor activator of nuclear factor-κB ligand (RANKL, an osteoclastogenesis-promoting factor) and osteoprotegerin (OPG, the decoy receptor for RANKL), oxidative stress and bone metabolism markers in OSAHS, in order to understand the potential mechanisms underlying bone loss in OSAHS patients.@*METHODS@#Forty-eight male patients with OSAHS, confirmed by polysomnography (PSG) study, were enrolled. Twenty male subjects who were confirmed as not having OSAHS served as the controls. The subjects' bone mineral density (BMD) was assessed in lumbar spine and femoral neck using dual-energy X-ray absorptiometry (DXA). Blood samples were collected from all subjects for measurement of RANKL, OPG, the bone formation marker bone-specific alkaline phosphatase (BAP), the bone resorption marker tartrate-resistant acid phosphatase 5b (TRAP-5b), and total antioxidant capacity (TAOC).@*RESULTS@#The BMD and the T-score of the femoral neck and the lumbar spine were significantly lower in OSAHS patients as compared to the control group (P  0.05). The serum level of OPG and the OPG/RANKL ratio were lower in the OSAHS group compared to the control group (both P < 0.05). TAOC level was also decreased significantly in the OSAHS group (P < 0.05). Correlation analysis showed that the TAOC level was positively correlated with BAP in the OSAHS group (r = 0.248, P = 0.04), but there were no correlations between TAOC and the BMD or the T-scores. The correlations between the level of OPG (or the OPG/RANKL ratio) and BMD or TAOC did not reach significance.@*CONCLUSION@#In OSAHS patients, lower levels of TAOC were associated with decreased bone formation, suggesting a role of oxidative stress in bone loss, while the role of OPG/RANKL imbalance in bone metabolism in OSAHS needs further evaluation.


Subject(s)
Absorptiometry, Photon , Adolescent , Adult , Bone Density , Physiology , Female , Humans , Male , Middle Aged , NF-kappa B , Blood , Osteogenesis , Physiology , Osteoporosis , Blood , Osteoprotegerin , Blood , Oxidative Stress , Physiology , Polysomnography , Sleep Apnea, Obstructive , Blood , Young Adult
13.
Article in English | WPRIM | ID: wpr-786198

ABSTRACT

BACKGROUND: Serum myostatin levels are increased according to renal function decline and myostatin may be a main mediator of chronic kidney disease–related sarcopenia. A previous study reported that serum myostatin level was negatively associated with abdominal aortic calcification (AAC) in older males. The aim of this study was to assess the association between serum myostatin level and AAC among dialysis patients of both sexes. In addition, we analyzed the relationship between serum myostatin level, muscle mass, and bone mineral density (BMD).METHODS: In this cross-sectional study, we evaluated AAC in the lateral lumbar spine using plain radiography and BMD in 71 patients undergoing dialysis. We classified patients into two groups according to the median value of myostatin as follows: those with high myostatin levels (≥ 5.0 ng/mL) and those with low myostatin levels (< 5.0 ng/mL).RESULTS: The proportion of patients with an AAC score of five points or more was higher among those with low myostatin levels. Myostatin level was negatively associated with AAC scores on plain radiography and had a positive association with skeletal muscle mass and T-scores for BMD measured at the total hip and femur neck. Lower myostatin levels were independently associated with higher AAC scores following adjustment for age, sex, diabetes mellitus, dialysis vintage, dialysis modality, and osteoprotegerin level.CONCLUSION: Lower serum myostatin levels were associated with higher AAC scores, lower muscle mass, and lower BMD in dialysis patients. Further, prospective studies and those with larger cohorts are necessary to validate these findings.


Subject(s)
Bone Density , Cohort Studies , Cross-Sectional Studies , Diabetes Mellitus , Dialysis , Femur Neck , Hip , Humans , Kidney , Male , Muscle, Skeletal , Myostatin , Osteoprotegerin , Prospective Studies , Radiography , Sarcopenia , Spine , Vascular Calcification
14.
Braz. j. med. biol. res ; 52(3): e8098, 2019. tab, graf
Article in English | LILACS | ID: biblio-984039

ABSTRACT

This aim of this study was to assess the molecular mechanism of osteoporosis in schizophrenia patients with risperidone use. Here, we investigated the effects of risperidone on cellular proliferation and apoptosis of a preosteoblast cell line, MC3T3-E1. Cell viability and apoptotic rate of MC3T3-E1 were detected by cell counting kit-8 and flow cytometry at a serial dose of risperidone and at different time points, respectively. Bone transformation relevant gene serum osteocalcin (BGP), collagen 1, tumor necrosis factor-α (TNF-α), osteoprotegerin (OPG), and receptor activator of nuclear factor-κB ligand (RANKL) mRNA levels were determined by real-time PCR (qPCR). Their protein expression patterns were evaluated using western blot. The results revealed that risperidone dramatically inhibited MC3T3-E1 cell proliferation in a dose-dependent manner. It also significantly induced MC3T3-E1 cell apoptosis. TNF-α gene and protein levels were greatly enhanced after risperidone treatment. In contrast, BGP, collagen 1, OPG, and RANKL gene and protein levels were markedly downregulated. Our study indicated that risperidone suppressed MC3T3-E1 cell proliferation and induced apoptosis. It also regulated BGP gene and protein expression.


Subject(s)
Animals , Osteoblasts/drug effects , Apoptosis/drug effects , Risperidone/pharmacology , Cell Proliferation/drug effects , Osteocalcin/drug effects , Cell Line , Collagen/drug effects , Tumor Necrosis Factor-alpha/drug effects , Receptor Activator of Nuclear Factor-kappa B/drug effects , Osteoprotegerin/drug effects , Flow Cytometry
15.
Braz. j. med. biol. res ; 52(12): e8754, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055467

ABSTRACT

Cuscuta chinensis Lam. (Convolvulaceae) is an important herbal medicine widely used to improve sexual function, treat osteoporosis, and prevent aging, and has been reported to exhibit anti-osteoporotic effects in vitro. However, the activity of Cuscuta chinensis Lam. on glucocorticoid-induced osteoporosis still remains unclear. The present study aimed to assess the protective effect and the underlying mechanism of action of Cuscuta chinensis extract (CCE) against glucocorticoid-induced osteoporosis in vivo. Sprague-Dawley rats were randomly divided into four groups as follows: control group, osteoporosis group, and 2 CCE-treated osteoporosis groups (100 mg·kg-1·day-1). Blood samples and femur bones were collected for immunohistochemistry, biochemical, mRNA expression, and western blot analysis. HPLC analysis revealed that chlorogenic acid, quercetin, and hyperin were the major constituents of CCE. The results indicated that CCE increased bone length, bone weight, and bone mineral density and suppressed dexamethasone (DEX)-induced reduction in body weight. In addition, TRAP staining indicated that CCE reduced osteoclasts in DEX-induced osteoporosis rats. Mechanistically, CCE treatment alleviated the increase of bone resorption markers and the decline of osteogenic markers, which might be partially mediated by regulation of RANKL/OPG and RunX2 pathways. These results suggest that CCE showed promising effects in the protection against glucocorticoid-induced osteoporosis through protecting osteoblasts and suppressing osteoclastogenesis.


Subject(s)
Animals , Rats , Osteoporosis/prevention & control , Dexamethasone/pharmacology , Plant Extracts/pharmacology , Cuscuta/chemistry , Osteoprotegerin/metabolism , Glucocorticoids/pharmacology , Osteoporosis/chemically induced , RNA, Messenger , Immunohistochemistry , Bone Density/drug effects , Blotting, Western , Chromatography, High Pressure Liquid , Rats, Sprague-Dawley , RANK Ligand/drug effects , RANK Ligand/metabolism , Osteoprotegerin/drug effects
16.
J. appl. oral sci ; 27: e20180316, 2019. tab
Article in English | LILACS, BBO | ID: biblio-984569

ABSTRACT

Abstract Objective The aim of this study was to evaluate the levels of salivary biomarkers IL-1β, IL-10, RANK, OPG, MMP-2, TG-β and TNF-α in individuals with diagnosis of peri-implant mucositis in the absence or presence of periodontal and peri-implant maintenance therapy (TMPP) over 5 years. Material and Methods Eighty individuals diagnosed with peri-implant mucositis were divided into two groups: one group that underwent periodontal and peri-implant regularly maintenance therapy, called GTP (n=39), and a second group that received no regular maintenance GNTP (n=41). Each participant underwent a complete periodontal and peri-implant clinical examination. Collection of saliva samples and radiographic examination to evaluate peri-implant bone levels were conducted at two times: initial examination (T1) and after 5 years (T2). The salivary samples were evaluated through ELISA for the following markers: IL-1β, IL-10, RANK, OPG, MMP-2, TGF and TNF-α. Results A higher incidence of peri-implantitis was observed in the GNTP group (43.9%) than in the GTP group (18%) (p=0.000). All individuals (n=12) who presented peri-implant mucositis and had resolution at T2 were in the GTP group. After 5 years, there was an increase in the incidence of periodontitis in the GNTP group compared to the GTP group (p=0.001). The results of the study revealed an increase in the salivary concentration of TNF-α in the GNTP group compared to the GTP group. The other salivary biomarkers that were evaluated did not show statistically significant differences between the two groups. Conclusions The salivary concentration of TNF-α was increased in individuals with worse periodontal and peri-implant clinical condition and in those with a higher incidence of peri-implantitis, especially in the GNTP group. Longitudinal studies in larger populations are needed to confirm these findings and elucidate the role of this biomarker in peri-implant disease.


Subject(s)
Humans , Periodontitis/pathology , Saliva/chemistry , Stomatitis/pathology , Dental Implants/adverse effects , Cytokines/analysis , Receptor Activator of Nuclear Factor-kappa B/analysis , Osteoprotegerin/analysis , Periodontitis/diagnosis , Reference Values , Stomatitis/diagnosis , Enzyme-Linked Immunosorbent Assay , Biomarkers/analysis , Case-Control Studies , Risk Factors , Follow-Up Studies , Statistics, Nonparametric , Disease Progression
17.
Rev. med. Rosario ; 84(1): 22-25, ene.-abr. 2018. ilus
Article in Spanish | LILACS | ID: biblio-973330

ABSTRACT

Una paciente con osteoporosis había sido tratada por 4 años con ibandronato oral, luego por 1 año con ranelato de estroncio, y finalmente por 4 años con denosumab. En vista de la buena respuesta densitométrica este fármaco fue suspendido a fines de 2015. A los 14 meses la enferma tuvo lumbalgia aguda y se detectó hundimiento del platillo superior de L1, a lo que siguieron en rápida sucesión iguales lesiones en L2 y L3, y acuñamiento de D11 y D12. Se descartaron causas de osteoporosis secundaria. El plan terapéutico incluye corsé ortopédico, analgésicos, y teriparatida. En los dos últimos años se han publicado varios casos de este síndrome.


A patient with osteoporosis had been treated for 4 years with oral ibandronate, then for 1 year with strontium ranelate, and finally for 4 years with denosumab. In view of the good densitometric response to the latter, the drug was discontinued in December 2015. Fourteen months later the patient had acute low back pain; crushing of the upper plate of L1 was detected, followed by similar lesions in L2 and L3, and wedging of D11 and D12. Causes of secondary osteoporosis were ruled out. The therapeutic strategy includes a corset, analgesics, and teriparatide. In the last two years several cases of this syndrome have been reported.


Subject(s)
Humans , Female , Aged, 80 and over , Antibodies, Monoclonal , Antibodies, Monoclonal/drug effects , Osteoporosis, Postmenopausal/prevention & control , Spinal Fractures/diagnosis , Spinal Fractures/prevention & control , Osteoprotegerin , Osteoprotegerin/drug effects , Treatment Outcome
18.
Braz. dent. j ; 29(1): 14-22, Jan.-Feb. 2018. tab, graf
Article in English | LILACS | ID: biblio-888726

ABSTRACT

Abstract tHistory of chronic periodontitis (CP) is a risk factor for oseointegration failure. The osteoclastogenesis system (RANK, RANKL and OPG) is critical for bone homeostatic control. We investigated the levels of OPG and RANKL in peri-implant tissues from volunteers with and without a history of CP and their association with mucosae inflammation. This is a single-blind case-contro study. Diagnosis of a history of CP and peri-implant examination was performed on 46 volunteers, divided into control (without history of CP, n=26) and CP group (with history of CP, n=20). Gingival biopsies were harvested during implant exposure. Quantitative PCR evaluated OPG/RANKL mRNA expressions. OPG and RANKL proteins were analyzed by western blot and immunohistochemistry assay. The chi-square test analyzed the significance of nominal variables between groups while continuous variables were analyzed by T-test or Mann-Whitney test, after Shapiro-Wilk test evaluation. The 2-ΔΔCT Livak method calculation evaluated the gene expression. Values of p<0.05 were considered statistically significant. Volunteers with CP history had 23 times higher chance of developing mucosae inflammation. High mucosae levels of RANKL (p=0.04) and RANKL/OPG (p=0.001) mRNA expressions were observed in CP group. CP volunteers showed increased RANKL protein levels in opposition to decreased OPG expression. Even without active periodontitis, volunteers with a history of CP had elevated gingival levels of RANKL/OPG and higher correlation with peri-implant mucosae inflammation and implant loss.


Resumo A história de periodontite crônica (CP) é um fator de risco para falhas na osseointegração. O sistema de osteoclastogênese (RANK, RANKL e OPG) é crucial para o controle da homeostase óssea. O objetivo deste estudo foi investigar os níveis de OPG e RANKL no tecido peri-implantar de voluntários com e sem histórico de CP e sua associação com inflamação da mucosa. Este é um estudo tipo caso-controle. O exame para diagnóstico de CP e na região peri-implantar foi realizado em 46 voluntários, divididos em controle (sem história CP, n=26) e grupo CP (com histórico de CP, n=20). Descartes gengivais foram obtidos durante a exposição do implante. PCR quantitativo avaliou a expressão do RNAm de OPG/RANKL. As proteínas OPG e RANKL foram analisadas por western blot e imunohistoquímica. O teste do qui-quadrado analisou a significância entre as variáveis nominais enquanto as variáveis contínuas foram analisadas pelo teste-t e Mann-Whitney, após o teste de Shapiro-wilk. O método do Livak 2--ΔΔCT avaliou a expressão gênica. Os voluntários com CP apresentaram 23 vezes mais chances de desenvolver inflamação da mucosa. Expressão elevada no RNAm de RANKL (p=0.04) e RANKL/OPG (p=0.001) foram observadas no grupo CP. Voluntários com CP mostraram aumento dos níveis da proteína RANKL em contraste com diminuída expressão de OPG. Mesmo sem periodontite ativa, voluntários com histórico de CP apresentaram elevado nível gengival de RANKL/OPG e alta correlação com inflamação peri-implantar e perda do implante.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Chronic Periodontitis/metabolism , Dental Implants , Mouth Mucosa/pathology , Osteoprotegerin/metabolism , RANK Ligand/metabolism , Blotting, Western , Chronic Periodontitis/pathology , Immunohistochemistry , Osteoprotegerin/genetics , Polymerase Chain Reaction , RANK Ligand/genetics , RNA, Messenger/genetics
19.
Araçatuba; s.n; 2018. 108 p. graf, ilus.
Thesis in Portuguese | LILACS, BBO | ID: biblio-915619

ABSTRACT

Objetivo: Avaliar o reparo ósseo periimplantar em ratas submetidas à ovariectomia e tratadas com Ranelato de Estrôncio (625mg/kg/dia) ou OPG-Fc (10mg/Kg/2 vezes por semana). Material e Método: Sessenta e quatro ratas, com peso aproximado de 300 gramas, divididas em 4 grupos experimentais conforme tratamento medicamentoso e análises: SHAM; OVX; OVX/RE; OVX/OPG-Fc. Cada animal recebeu 2 implantes sendo 1 em cada metáfise tibial. A eutanásia foi realizada aos 42 e 60 dias após a instalação dos implantes. Foram realizadas as análises de microtomografia computadorizada (micro CT), biomecânica (torque reverso) histométrica, por microscopia confocal a laser, análise da expressão gênica por PCR em tempo real, histológica e imunoistoquímica. Os resultados histológicos evidenciaram para o grupo OVX-RE aspecto de neoformação óssea melhorada, com trabéculas mais espessas e baixa presença de tecido conjuntivo, comparado ao grupo OVX. Os resultados imunoistoquímicos demonstraram intensa marcação de OPG para o grupo OVX-RE e intensa marcação de RANKL para o grupo OVX. Já a análise por microscopia confocal evidenciou que o grupo OVX-RE obteve marcação superior para vermelho de alizarina comparado aos outros dois grupos (Tukey test ­ P < 0,05). Para os parâmetros BV/TV, Tb.Th, o grupo OVX/RE apresentou os maiores valores em relação aos demais grupos. Para Tb.Sp o grupo OVX/OPG-Fc apresentou o melhor resultado (OVX: p<0,05 e OVX/RE: p>0,05), e Tb.N, houve diferença estatisticamente significante na comparação dos resultados entre os grupos OVX e OVX/OPG-Fc (p<0,05). Para MAR e avaliação dos fluorocromos, OVX/RE mostrou melhor turnover ósseo periimplantar, para AON, o grupo SHAM apresentou os maiores resultados (OVX: p<0,05), e para ELCOI o grupo OVX/RE apresentou os melhores resultados. Concluiu-se que o tratamento com ranelato de estrôncio ou OPG-Fc humano melhora significativamente a reparação óssea periimplantar de ratas osteopênicas por inibição da atividade osteclástica exacerbada, promovendo um equilíbrio entre OPG/RANKL, apresentando essa característica melhorada nos animais tratados com ranelato de estrôncio(AU)


Objectives: Evaluate the peri-implant bone repair in rats submitted to ovariectomy and treated with Strontium Ranelate (625mg/kg/day) or OPG-Fc (10mg/kg/2 times per week). Material and Method: sixty-four rats weighing about 300 grams, they were divided into 4 experimental groups according with the treatment and analysis: SHAM; OVX; OVX/RE; OVX/OPG-Fc. Each animal received 2 implants, 1 in each tibial metaphysis. Euthanasia was performed 42 and 60 days after implant installation. Computed micro-tomography (micro-CT), biomechanical (reverse torque), histometrical, laser confocal microscopy, realtime gene expression (RT-PCR), histology and immunohistochemiscal analysis. The histological results revealed for the OVX-RE group a better bone neoformation with thicker trabeculae and low connective tissue compared to OVX group. The immunohistochemical results showed intense labeling of OPG to the OVX-RE group and intense labeling of RANKL to the OVX group. Confocal microscopy analysis exhibited that the OVX-RE group obtained a greater red alizarin marking compared to the others two groups (Tukey test - p<0.05). For the parameters BV/TV, Tb.Th, the OVX/RE group showed the larger values in relation to the other groups. For Tb.Sp the OVX/OPG-Fc group presented the best result (OVX: p<0.05 and OVX/RE: p> 0.05), and the Tb.N exhibited a statistical significant difference in the combination of results between the OVX and OVX / OPG-Fc groups (p<0.05). For the MAR and fluorochromes evaluation, OVX/RE group showed a better peri-implant bone turnover, for the AON, the SHAM group presented the higher results (OVX: p<0.05), and for the ELCOI, the OVX/RE group presented the best results. It is concluded that the treatment with strontium ranelate or OPG-Fc human improve significantly the peri-implant bone repair of osteopenic rats by inhibiting the exacerbated osteoclast activity, promoting a balance between the OPG/RANKL, it showing this improved characteristic in the animals treated with the strontium ranch(AU)


Subject(s)
Animals , Rats , Bone Regeneration , Dental Implants , Osteoporosis , Estrogens , Immunohistochemistry , Osteoprotegerin , Rats, Wistar
20.
Braz. J. Pharm. Sci. (Online) ; 54(3): e17567, 2018. graf
Article in English | LILACS | ID: biblio-974397

ABSTRACT

In this study, the effects of geraniin on osteoprotegerin/receptor activator of nuclear factor-κB ligand(OPG/RANKL) in regulating the proliferation of osteoblasts and suppression of osteoclast-like cells (OLC) in OLC-osteoblast co-cultured system in vitro were investigated. Osteoblasts were cultured and identified with alkaline phosphatase (ALP), gomori stain, and mineralized nodule stain. OLCs were isolated from long bones of Sprague-Dawley (SD) rats and identified with tartrate-resistant acid phosphatase(TRAP) stain. Methyl thiazolyl tetrazolium assay was used to examine the proliferation of osteoblasts, and immunocytochemistry and in situ hybridization to analyze the expression OPG/RANKL in osteoblasts co-cultured with osteoclasts under the action of geraniin, respectively. Geraniin could regulate the proliferation of osteoblasts MC3T3-E1, decrease the number of OLC in OLC-osteoblast co-cultured system, and inhibit the bone resorption areas and resorption pits of OLC in vitro experiments. Geraniin could promote the mRNA and protein expression levels of OPG and suppress those of RANKL in osteoblasts. These results indicate that geraniin has a promoting effect on the proliferation of osteoblasts and an inhibitory effect on the osteoclastic bone-resorption through regulating OPG/RANKL signaling pathway in OLC-OB co-cultured system.


Subject(s)
Animals , Male , Female , Rats , RANK Ligand/classification , Osteoprotegerin/adverse effects , Osteoblasts , Phyllanthus/classification , Plant Components, Aerial
SELECTION OF CITATIONS
SEARCH DETAIL