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1.
Article in Chinese | WPRIM | ID: wpr-771725

ABSTRACT

This study aimed to investigate the molecular mechanism and protective effect of total saponins of Panax japonicas (TSPJ) on HepG2 cells apoptosis induced by palmitic acid (PA).The HepG2 cells were cultured , and divided into five groups: the control group, the model group, the high-dose group (50 mg·L⁻¹), the middle-dose group (25 mg·L⁻¹) and the low-dose group (12.5 mg·L⁻¹).The cells of the five groups were cultured continuously for 24 hours. The cell viability was measured with MTT. HepG2 cells apoptosis was detected by Hoechest staining and Annexin V-FITC/PI staining. The protein expressions of BCL-2, CHOP and TLR4 were measured with western blotting and flow cytometry analysis. The mRNA expressions of TNF-α, IL-1β, BCL-2, CHOP and GAPDH were measured with RT-PCR. The results suggested that compared with the control group, the number of HepG2 cells of the model group were reduced significantly (<0.01), while the number of apoptotic HepG2 cells were increased. Compared with the model group, the number of HepG2 cells of the high-dose group and the middle-dose group were increased significantly (<0.01), whereas the number of apoptotic HepG2 cells were reduced. Compared with the control group, TNF-α, IL-1β and CHOP mRNA expressions and CHOP and TLR4 protein expressions in the model group were significantly up-regulated (<0.01), while BCL-2 protein and mRNA expressions in the model group were significantly decreased (<0.01). Compared with the model group, TNF-α, IL-1β and CHOP mRNA expressions and CHOP and TLR4 protein expressions in the high-dose group were significantly decreased (<0.01), while BCL-2 protein and mRNA expressions in the high-dose group were significantly up-regulated (<0.01).In conclusion, TSPJ can reduce inflammation and apoptosis induced by palmitic acid, with a certain protective effect on liver cells.


Subject(s)
Apoptosis , Hep G2 Cells , Humans , Palmitic Acids , Panax , Chemistry , Phytochemicals , Pharmacology , Saponins , Pharmacology
2.
Article in English | WPRIM | ID: wpr-728311

ABSTRACT

The structures of the intact synaptosomal plasma membrane vesicles (SPMVs) isolated from bovine cerebral cortexs, and the outer and the inner monolayer separately, were evaluated with 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1,3-di(1-pyrenyl)propane (Py-3-Py) as fluorescent reporters and trinitrophenyl groups as quenching agents. The methanol increased bulk rotational and lateral mobilities of SPMVs lipid bilayers. The methanol increased the rotational and lateral mobilities of the outer monolayers more than of the inner monolayers. n-(9-Anthroyloxy)stearic acid (n-AS) were used to evaluate the effect of the methanol on the rotational mobility at the 16, 12, 9, 6, and 2 position of aliphatic chains present in phospholipids of the SPMVs outer monolayers. The methanol decreased the anisotropy of the 16-(9-anthroyloxy)palmitic acid (16-AP), 12-(9-anthroyloxy)stearic acid (12-AS), 9-(9-anthroyloxy)stearic acid (9-AS), and 6-(9-anthroyloxy)stearic acid (6-AS) in the SPMVs outer monolayer but it increased the anisotropy of 2-(9-anthroyloxy)stearic acid (2-AS) in the monolayers. The magnitude of the increased rotational mobility by the methanol was in the order at the position of 16, 12, 9, and 6 of aliphatic chains in phospholipids of the outer monolayers. Furthermore, the methanol increased annular lipid fluidity and also caused membrane proteins to cluster. The important finding is that was far greater increase by methanol in annular lipid fluidity than increase in lateral and rotational mobilities by the methanol. Methanol alters the stereo or dynamics of the proteins in the lipid bilayers by combining with lipids, especially with the annular lipids. In conclusion, the present data suggest that methanol, in additions to its direct interaction with proteins, concurrently interacts with membrane lipids, fluidizing the membrane, and thus inducing conformational changes of proteins known to be intimately associated with membranes lipids.


Subject(s)
Anisotropy , Cell Membrane , Cerebral Cortex , Diphenylhexatriene , Lipid Bilayers , Membrane Lipids , Membrane Proteins , Membranes , Methanol , Neurons , Palmitic Acids , Phospholipids , Proteins , Stearic Acids
3.
Article in Chinese | WPRIM | ID: wpr-274296

ABSTRACT

<p><b>OBJECTIVE</b>To establish a new rapid method to screen potential hepatoprotective compounds from traditional Chinese medicine, and identify the hepatoprotective compounds in Paeoniae Radix Rubra.</p><p><b>METHOD</b>Fluorescein diacetate labelled and MTT assay were applied for screening the hepatoprotective fractions on HepG2 cells exposed to galactosamine. The active fractions were analyzed by chromatography coupled with mass spectrometry. Finally, the hepatoprotective effects of the identified compounds were validated by hepatoprotective assay.</p><p><b>RESULT</b>Three hepatoprotective fractions were founded, in which three compounds were identified as paeoniflorin, ethyl palmitate and ethyl linoleate. Validation results indicated that all the three compounds can attenuate the galactosamine induced injury on HepG2 cells.</p><p><b>CONCLUSION</b>Paeoniflorin, ethyl palmitate and ethyl linoleate from paeoniae radix rubra showed potential hepatoprotective activity.</p>


Subject(s)
Benzoates , Pharmacology , Bridged-Ring Compounds , Pharmacology , Glucosides , Pharmacology , Hep G2 Cells , Humans , Linoleic Acids , Pharmacology , Liver , Monoterpenes , Paeonia , Chemistry , Palmitic Acids , Pharmacology , Protective Agents
4.
Article in English | WPRIM | ID: wpr-92236

ABSTRACT

To provide a basis for studying the pharmacological actions of tetracaine.HCl, we analyzed the membrane activities of this local anesthetic. The n-(9-anthroyloxy) stearic and palmitic acid (n-AS) probes (n = 2, 6, 9, 12 and 16) have been used previously to examine fluorescence polarization gradients. These probes can report the environment at a graded series of depths from the surface to the center of the membrane bilayer structure. In a dose-dependent manner, tetracaine.HCl decreased the anisotropies of 6-AS, 9-AS, 12-AS and 16-AP in the hydrocarbon interior of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex (SPMV), and liposomes derived from total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from the SPMV. However, this compound increased the anisotropy of 2-AS at the membrane interface. The magnitude of the membrane rotational mobility reflects the carbon atom numbers of the phospholipids comprising SPMV, SPMVTL and SPMVPL and was in the order of the 16, 12, 9, 6, and 2 positions of the aliphatic chains. The sensitivity of the effects of tetracaine.HCl on the rotational mobility of the hydrocarbon interior or surface region was dependent on the carbon atom numbers in the descending order 16-AP, 12-AS, 9-AS, 6-AS and 2-AS and on whether neuronal or model membranes were involved in the descending order SPMV, SPMVPL and SPMVTL.


Subject(s)
Anisotropy , Carbon , Cell Membrane , Cerebral Cortex , Fluorescence Polarization , Liposomes , Membranes , Neurons , Palmitic Acid , Palmitic Acids , Phospholipids , Stearic Acids
5.
Acta Pharmaceutica Sinica ; (12): 1095-1101, 2009.
Article in Chinese | WPRIM | ID: wpr-344051

ABSTRACT

The global metabolite profiles of endogenous compounds of Wistar rats from 12 to 20 weeks old were investigated to take deep insight into and get better understanding of the pathogenesis of development and aging. Plasma from Wistar rats at 12, 14, 16, 18, and 20 weeks old were analyzed using GC/TOFMS. Multivariate data analysis was then used to process the metabonomic data which indicated excellent separation between different weeks and showed that the metabolic profiles of the samples changed with age, enabling age-related metabolic trajectories to be visualized. Decreased concentrations of citric acid, cis-aconitic acid, 9-(z)-hexadecenoic acid along with increased levels of hexanedioic acid, alpha-tocopherol, 3-indole propionic acid, etc contributed to the separation. Several major metabolic pathways were identified to be involved in metabolic regulation. This suggests that GC/TOFMS-based metabonomics is a powerful alternative approach to identifying potential biomarkers and investigating the physiological developments of aging and it is important to employ suitable age-match control group in metabonomic study of physiological monitoring, drug safety assessment, and disease diagnosis, etc.


Subject(s)
Aconitic Acid , Blood , Adipates , Blood , Aging , Blood , Physiology , Animals , Biomarkers , Blood , Chromatography, Gas , Methods , Citric Acid , Blood , Indoles , Blood , Male , Metabolome , Metabolomics , Multivariate Analysis , Palmitic Acids , Blood , Propionates , Blood , Rats , Rats, Wistar , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Methods , alpha-Tocopherol , Blood
6.
Article in Korean | WPRIM | ID: wpr-655696

ABSTRACT

Studies on the relationship between blood fatty acids and the risk of breast cancer have not yielded definite conclusions. The role of fatty acids in the development and progression of breast cancer is unclear. We conducted a case-control study to determine serum phospholipid fatty acid composition in benign breast tumor and breast cancer. Subjects consisted of 27 benign breast tumor and 68 breast cancer patients, and 28 matched controls. The levels of fatty acids were measured by gas chromatography. Higher arachidonic and palmitic acids were observed in breast cancer patients as compared with control and benign breast tumor patients. The percentage of total saturated fatty acids in breast cancer was higher than in control and benign breast tumor patients. The level of stearic acid was lower in benign breast tumor and breast cancer patients. Saturation index, the ratio of stearic to oleic acid, was lower in benign breast tumor and breast cancer patients compared to the control. Moreover, stearic acid was negatively and arachidonic acid was positively correlated with the cancer stage. In conclusion, our results support that serum phospholipid compositions of specific fatty acids are associated with the risk of benign breast tumor as well as breast cancer. Further studies are necessary to investigate mechanisms linked to the breast cancer etiology.


Subject(s)
Arachidonic Acid , Breast , Breast Neoplasms , Case-Control Studies , Chromatography, Gas , Fatty Acids , Humans , Oleic Acid , Palmitic Acid , Palmitic Acids , Stearic Acids
7.
Article in Chinese | WPRIM | ID: wpr-263081

ABSTRACT

<p><b>OBJECTIVE</b>To study the chemical constituents in roots of Peucedanum praeruptorum.</p><p><b>METHOD</b>The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods.</p><p><b>RESULT</b>Seven compounds, alpha-D-glucopyranose-1-hexadecanoate (1), D-mannitol monohexadecanoate (2), adenosine (3), butyric acid (4), eleutheroside B, (5), apiosylskimmin (6), and mannitol (7) were isolated and identified.</p><p><b>CONCLUSION</b>Compounds 1-5 were isolated from this plant for the first time.</p>


Subject(s)
Adenosine , Chemistry , Apiaceae , Chemistry , Butyrates , Chemistry , Drugs, Chinese Herbal , Chemistry , Glucosides , Chemistry , Mannitol , Chemistry , Palmitic Acids , Chemistry , Phenylpropionates , Chemistry , Plant Roots , Chemistry
8.
Acta Pharmaceutica Sinica ; (12): 292-296, 2007.
Article in English | WPRIM | ID: wpr-281905

ABSTRACT

A new compound and twelve known compounds were isolated from the ethyl acetate extract of the roots of Homonoia riparia Lour, which are used in folk medicine for treatment of hepatitis, bellyache and scald, by the method of silica gel column chromatography repeatedly with a gradient of PE-EtOAc, PE-Me2CO, CHCl3-Me2CO, CHCl3-MeOH. Their structures were identified as a new compound 1-oxo-aleuritolic acid (1), and twelve known compounds aleuritolic acid (2), 3-acetoxy-aleuritolic acid (3), taraxerone (4), taraxerol (5), methyl 3-acetoxy-12-oleanen-28-oate (6), 3-acetoxy-12-oleanen-28-ol (7), ursolic acid (8), lupenol (9), 3beta-acetoxy-lupenol (10), cleomiscosin A (11), chrysophanol (12), and gallic acid (13), which were obtained from this plant for the first time, by the spectroscopic techniques of NMR, HMBC, IR and MS, separately. Among the cytotoxicities evaluation of compounds 1 -3 towards AGZY 83-a (human lung cancer cells) and SMMC-7721 (human liver cancer cells) tumor cells was assayed by MTT methods with cis-dichlorodiamminoplatinum (DDP) used as positive control. Compound 2 exerted weak activity against AGZY 83-a with the IC50 value of 33.055 microg x mL(-1), while 1 and 3 showed no activity to these two cell lines.


Subject(s)
Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Cell Line, Tumor , Cell Survival , Coumarins , Dioxanes , Chemistry , Pharmacology , Euphorbiaceae , Chemistry , Humans , Inhibitory Concentration 50 , Molecular Structure , Oleanolic Acid , Chemistry , Pharmacology , Palmitic Acids , Chemistry , Pharmacology , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , Chemistry , Pharmacology
9.
Rev. Inst. Adolfo Lutz ; 65(1): 7-14, jan.-abr. 2006. tab
Article in Portuguese | LILACS, SES-SP, SESSP-IALPROD, SES-SP | ID: lil-450799

ABSTRACT

No Brasil tem ocorrido crescimento vertiginoso na criação de peixes de cativeiro de água doce; no entanto existem poucos estudos sobre identificação e quantificação de ácidos graxos em pescados provenientes de cativeiro. Sabe-se que entre diversos fatores, a dieta alimentar do peixe é o fator determinante sobre sua composição lipídica, especialmente quanto aos componentes de ácidos graxos. O objetivo deste trabalho foi de efetuar a revisão de vários estudos, com o intuito de obter um panorama sobre a composição dos ácidos graxos sem pescado e, ainda, quanto à influência da dieta sobre a composição de ácidos graxos dos peixes capturado sem ambiente natural e dos cultivados em diferentes sistemas. A maioria dos estudos aponta que a composição de ácidos graxos em peixes marinhos apresenta maiores proporções de ácidos graxos poliinsaturados Omega 3(AGPI-3) do que os peixes de água doce. O ácido graxo palmítico e o oléico atingem os mais altos níveis no pescado de água marinha e de água doce. Na maioria dos peixes, o DHA, o EPA e a-linolênico são as maiores fontes de AGPI3. Enquanto o linoléico e o araquidônico contribuem como fonte de AGPI6.


Subject(s)
Lipids , Fishes , Fatty Acids , Oleic Acids , Palmitic Acids , Diet
10.
Article in Chinese | WPRIM | ID: wpr-327166

ABSTRACT

The main component of this pill is 2-Octadecanoic acid-4-Palmitic acid-2, 4-Pentanediyl ester separated from chloroform extract of neem oil. The microcapsules coated by the re-curdle method were fabricated with an average particle size of 100-180 microm. The morphological characteristics, incorporation efficiency, carrier reclamation efficiency of the microcapsule were investigated. Kunming mice were used in the experiment, and the anti-fertility effect of the microcapsule on the histology and apoptosis was studied by light and electron microscopy and the flow cytometry. The data obtained clearly indicated that the microcapsule could lead to the payload of medicine, the incorporation efficiency being 90%. After the microcapsules were given to the male mice orally, its anti-fertility effect came into being and could keep the mice in a state of reversible infertility for a long time. The results of histological study and flow cytometry indicate that the mechanism of its anti-fertility effect involves mainly the inhibition of sperm motility and the arrest of spermatogenic process.


Subject(s)
Animals , Capsules , Contraceptive Agents, Male , Pharmacology , Drug Compounding , Glycerides , Chemistry , Male , Mice , Palmitic Acids , Pharmacology , Particle Size , Sperm Motility , Spermatogenesis , Stearic Acids , Pharmacology , Terpenes , Chemistry
11.
Mansoura Journal of Pharmaceutical Sciences. 1998; 14 (1): 71-87
in English | IMEMR | ID: emr-48634

ABSTRACT

This study investigated the mechanism by which anhydrous calcium phosphate [A-TAB] exhibits its sustained release matrix, via premixing of A-TAB [diluent] powder with different excipients, e.g. Avicel, sodium alginate and polyvinyl alcohol [PVA], to block entrapment sites if any, on the dissolution of theophylline from A-TAB tablets. Furthermore, the inclusion of other excipient [palmetic acid] to impart a more acid resistant character on the diluent/drug blend and thus assist in maintaining the matrix structure of A-TAB were also investigated. The rate and extent of drug dissolution were dependent on the order of mixing of A-TAB with the drug and excipient as well as the pH of the dissolution medium


Subject(s)
Calcium Phosphates/pharmacokinetics , Delayed-Action Preparations , Drug Design , Polyvinyl Alcohol , Palmitic Acids , Theophylline
12.
Rev. ciênc. farm ; 19(2): 235-41, 1998. tab
Article in Portuguese | LILACS | ID: lil-229482

ABSTRACT

O fracionamento cromatográfico do extrato hexânico das partes aéreas de Cambessedesia espora DC - Melastomataceae levou ao isolamento dos ácidos palmítico, mirístico e esteárico, ß-Silosterol e do triterpeno fern-9(11)-en-3ß-ol. As substâncias foram identificadas por métodos espectroscópicos usuais (RMN, IV, CG/EM).


Subject(s)
Plant Extracts/chemistry , Plants/chemistry , Myristic Acid/isolation & purification , Stearic Acids/isolation & purification , Fatty Acids/isolation & purification , Palmitic Acids/isolation & purification , Chromatography, Thin Layer , Spectrophotometry , Spectrophotometry, Infrared , Triterpenes/isolation & purification
13.
Article in English | IMSEAR | ID: sea-112108

ABSTRACT

Ethyl palmitate (EP) was used as a macrophage cytotoxin. The response of P. berghei after exposing the macrophage to EP was opposite to what was seen with other agents like Silica, Antimacrophage serum and Freund's complete adjuvant. EP at dose of 5 mg and above decreased the survival period (SP), median survival day (MSD) and parasite density 24 hrs. before death (K values). Prepatent period (PP) was lower at doses 10 mg and 20 mg per day for 5 days before challenge compared to their corresponding controls. EP at a dose of 5 mg and above was found to be toxic to host, mice. EP in dosage of 3 mg per mouse administered 48 hrs. before challenge resulted in an increase in the mean survival period, survival rate (30%) and decrease in the mean parasitaemia per day when compared with the corresponding control. The interfering agents affected differently both the host and/or parasite. A proper modulation of the macrophage during the course of infection may help the host in surviving this lethal infection.


Subject(s)
Animals , Cytotoxins/pharmacology , Disease Models, Animal , Macrophages/drug effects , Malaria/drug therapy , Male , Mice , Palmitic Acids/pharmacology , Plasmodium berghei , Survival Analysis
14.
Indian J Biochem Biophys ; 1996 Oct; 33(5): 343-8
Article in English | IMSEAR | ID: sea-27716

ABSTRACT

The biochemistry and cell biology of covalent attachment of the fatty acids palmitic and myristic to proteins has been the subject of extensive investigations during the past fifteen years. While the site of attachment of fatty acids and the primary structure of proteins around the acylation site have been extensively documented, the exact role of the fatty acids have only been speculated upon. Since fatty acids would prefer to be associated with the lipid bilayer of membranes, it has been assumed that the role of the fatty acid is to provide a stable membrane anchor. This review discusses recent reports in the area of fatty acylation which suggests roles for the fatty acid other than that of a stable membrane anchor.


Subject(s)
Acylation , Acyltransferases/metabolism , Lipid Bilayers/chemistry , Membrane Proteins/chemistry , Models, Chemical , Myristic Acids/metabolism , Palmitic Acids/metabolism , Proteins/chemistry
15.
Acta physiol. pharmacol. ther. latinoam ; 46(2): 57-69, 1996. tab, graf
Article in English | LILACS | ID: lil-172310

ABSTRACT

It is well-known that ethanol alters fatty acid and glycerolipid metabolism in liver, but most of the studies have been deleloped on rats, so little known about the corresponding effects on human liver. We have chosen the Hep G2 human hepatoma cell line, which appears be an excellent in vitro model system. Cells were incubated in ethanol containing medium (0-400 mM) for 48 h. Incorporation and metabolism of radioactive substrates (14C(U) glycerol, [1-(14)C] palmitic acid and [1-(14)C] eicosatrienoic acid (n-6) were analyzed in cellular and conditioned medium lipids. Cellular growth rate and lipid composition of control and ethanol-treated cells were also studied. The results showed that ethanol inhibited logarithmic cellular growth rate in a concentration dependent manner, without affecting viability. Ethanol (400 mM) did not modify cellular major lipid composition except for an increase of cholesteryl esters, but produced a decrease in the proportions of myristic, palmitic and palmitoleic acids. Ethanol enchanced the incorporation of radioactive fatty acids into cellular glycerolipids but did not alter the rate of incorporation of 14C(U) glycerol. This was attributed to an isotopic solution of the radioactive glycerol as a result of increasde alfa-glycerophosphate biosynthesis. Incorporation of radioactive fatty acids and glycerol into conditioned medium glycerolipids were increased in cells incubated in presence of ethanol. The increased incorporation of 14C glycerol into conditioned medium together with a simultaneous diminuition in labeling cellular glycerides suggest that there would be a simulation of the export of these lipid classes to conditioned medium. Conversion of [1-(14)C] palmitic to oleic acid and eicosatrienoic to arachidonic acid were inhibited in 400 mM ethanol treated cells suggesting an inhibition of delta 9 and delta 5 desaturase activity.


Subject(s)
Humans , /analogs & derivatives , Fatty Acids/metabolism , Carcinoma, Hepatocellular/metabolism , Ethanol/pharmacology , Glycolipids/metabolism , Liver Neoplasms/metabolism , /metabolism , Palmitic Acids/metabolism , Carcinoma, Hepatocellular/pathology , Glycerol/metabolism , Glycolipids/antagonists & inhibitors , Liver Neoplasms/pathology , Tumor Cells, Cultured
16.
Hansen. int ; 19(1): 17-27, jul. 1994. tab
Article in English | LILACS | ID: lil-178592

ABSTRACT

Cultivation trials for Mycobacterium leprae resulted in growth of Mycobacterium psychrophilum (L). Media were inoculated with host grown Mycobacterium leprae cells from armadillo tissues, Nu mice foot pads or human lepromata. Cultures were obtained in liquid and on semisolid multifactoria 1 media containing water soluble palmitic acid or its salts. Ammonium thioglycolate and Napalmitate served as carbon and energy sources. The water soluble palmitic acid remained in perfect solution following sterilization in the autoclave, thus easily accessible to the cells. The cyclodextrin-Fe complex served as a siderophore to grow the obtained leprosy derived psychrophilic cells. The leprosy derived cultures and subcultures grew opimally at+10 degrees Celssius but deteriorated rapidly at + 32 degrees Celsius, in the multifactorial media. No growth occurred in 7H9 media. Cultures were not identified for classification.


Subject(s)
Humans , Animals , Mice , Leprosy/microbiology , Mycobacterium leprae/isolation & purification , Mycobacterium/growth & development , Palmitic Acids , Culture Media , Leprosy, Tuberculoid/microbiology , Leprosy, Lepromatous/microbiology , Mycobacterium avium/growth & development , Mycobacterium avium/isolation & purification , Mycobacterium leprae/growth & development , Mycobacterium phlei/growth & development , Mycobacterium phlei/isolation & purification , Mycobacterium scrofulaceum/growth & development , Mycobacterium scrofulaceum/isolation & purification , Mycobacterium/isolation & purification
17.
Braz. j. med. biol. res ; 27(2): 239-42, Feb. 1994. ilus
Article in English | LILACS | ID: lil-140258

ABSTRACT

Two glycoinositol phospholipids (GIPL A and GIPL B) have been purified from epimastigotes of Trypanosoma cruzi at the logarithmic phase of growth (2 days). The GIPLs differ mainly in the lipid moiety and are similar to the lipopeptidophosphoglycan (LPPG) previously isolated from epimastigotes at the stationary phase (4-5 days). [3H]-palmitic acid was incorporated into 1-O-hexadecyl-2-O-palmitoylglycerol in GIPL A and into a sphinganine ceramide with palmitic acid and lignoceric acid as the fatty acids in GIPL B. The lipids could be released by incubation with phosphatidylinositol-specific phospholipase C (PI-PLC) or glycosylphosphatidylinositol phospholipase D (GPI-PLD) from rat serum. The oligosaccharides share the common core structure of the glycosylphosphatidilinositol (GPI) membrane anchors. Microheterogeneity was demonstrated, as well as substitution by galactose, which is mainly in the furanose configuration as was previously described for the LPPG. However, methylation analysis indicated that 20 percent of the galactose is present as terminal pyranose units. In infective trypomastigotes, [3H]-palmitic acid was incorporated into the anchor of the Tc-85 glycoprotein. The lipid cleaved by phospholipase C digestion was identified as 1-O-hexadecylglycerol and the main oligosaccharide has the structure of the conserved core of all GPI anchors. [3H]-palmitic acid-labelled Tc-85 released into the culture medium as membrane vesicles showed 80 percent resistance to the action of PI-PLC. However, after mild alkaline hydrolysis, part of the radioactivity was released by the enzyme


Subject(s)
Animals , Rats , Glycosphingolipids/chemistry , Oligosaccharides/chemistry , Trypanosoma cruzi/chemistry , Carbohydrate Sequence , Fatty Acids , Glycosphingolipids/isolation & purification , Molecular Sequence Data , Oligosaccharides/analysis , Oligosaccharides/isolation & purification , Palmitic Acids , Peptidoglycan/chemistry , Peptidoglycan/isolation & purification , Phospholipids/chemistry , Phospholipids/isolation & purification , Type C Phospholipases
18.
Bol. Centro Pesqui. Process. Aliment ; 11(2): 103-12, jul.-dez. 1993. tab
Article in Portuguese | LILACS | ID: lil-147482

ABSTRACT

Determinou-se o teor de acidez (ácido palmítico), o índice de peróxido, o índice de iodo, o número de TBA e o índice de refraçäo, de óleo de dendê bruto, óleo de caiaué e óleo de dendê refinado, provenientes de diferentes Estados brasileiros. Avaliou-se também a estabilidade do óleo bruto e do óleo refinado, armazenados a temperatura ambiente (26ºC) e em geladeira (5ºC). Os resultados obtidos mostraram valores elevados para o índice de refraçäo (1,4551 a 1,4680) e para o teor de acidez (2,36 e 24,66 por cento de ácido palmítico). Com relaçäo ao número de TBA, os valores situaram-se entre 1,486 e 6,935 x 10 em soluçäo de tetraetoxipropano (TEP). Quanto ao índice de peróxido (1,94 a 5,95 meg/kg), os valores encontrados estavam abaixo do limite estabelecido pela legislaçäo brasileira. Durante o experimento para a verificaçäo do estado de oxidaçäo dos óleos brutos e do óleo refinado, detectou-se um aumento significativo dos índice de peróxido, tanto a temperatura ambiente quanto em geladeira. Este mesmo comportamento foi verificado com o número de TBA


Subject(s)
Chemical Oxidation , Environmental Health , Palm Oil , Palmitic Acids , Plant Oils , Iodine , Peroxides
19.
Arch. med. res ; 24(4): 327-31, dez. 1993. tab
Article in English | LILACS | ID: lil-177011

ABSTRACT

In the present study the influence of age on red blood cell fatty acid (RBCFA) composition was analyzed in a sample of Mexico City children and young people on a free diet, as there is scarce information about RBCFA composition in the Mexican population. Erythrocyte lipids were extracted with isopropyl alcohol and fatty acid methyl esters were prepared to be analyzed by gas liquid chromatography. The 1. to 2-year-old group showed a higher percent level of C18:0 (34.73 ñ 2.5 vs. 29.67 ñ 1.3, p<0.002) and lower of C16:1 (0.58 ñ 0.2 vs. 1.09 ñ 0.2, p<0.005), C20:4 (14.08 ñ 4.1 vs, 18.20 ñ 1.2, p<0.05) and C22:5 (2.79 ñ 1.7 vs. 7.68 ñ 0.8, p<0.001) than the 20- to 25-year-old group. Both groups showed a very low linoleic acid proportion, children 0.48 percent and young adults 0.54 percent. The unsaturated/saturated fatty acid ratio was found to be 0.55 ñ 0.2 in children and 0.91 ñ 0.1 in adults (p<0.001). These findigs indicate the presence of factors related to age that affect the fatty acid composition in the erythrocyte membrane different from diet habits in the sample analyzed. Results are compared with reports in the literature


Subject(s)
Humans , Male , Female , Adolescent , Adult , Palmitic Acids/metabolism , Fatty Acids/metabolism , Erythrocyte Membrane/metabolism , Palmitates/metabolism
20.
Rev. farm. bioquim. Univ. Säo Paulo ; 29(1): 35-9, jan.-jun. 1993. tab
Article in Portuguese | LILACS | ID: lil-128652

ABSTRACT

Foi estudado o efeito da adicao de acido palmitico livre em relacao a estabilidade do azeite de dende, no armazenamento a temperatura de 60 graus centigrados. Foram avaliados a acidez, o indice de peroxidos e a formacao de compostos volateis totais. Verificou-se aumento no teor de acidos graxos livre durante o armazenamento. A presenca de acido palmitico livre no azeite de dende favoreceu a decomposicao dos hidroperoxidos, verificada pela formacao acentuada de compostos volateis totais


Subject(s)
Fatty Acids/analysis , Palmitic Acids/analysis , Food Chemistry , Plant Oils/analysis , Food Analysis , Food Quality , Food Technology
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