Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 118
Filter
1.
Electron. j. biotechnol ; 44: 1-5, Mar. 2020. graf, tab
Article in English | LILACS | ID: biblio-1087706

ABSTRACT

Background: Freeze-drying is known as one of the best methods to preserve bacterial strains. Protectant is the key factor affecting the survival rate of freeze-dried strains. In addition, salinity, bacterial suspension concentration, drying time, and other factors can also affect the survival rate of strains to varying degrees. At present, there are relatively few studies on freeze-drying preservation of marine bacteria. In the present study, we performed the freeze-drying protectant screening and optimized the preservation conditions for Pseudoalteromonas nigrifaciens, which is widely distributed in marine environment. The protective effects of the screened protectants were verified by 18 other marine bacterial strains. Results: The results indicated that the combination of 5.0% (w/v) lactose, 5.0% (w/v) mannitol, 5.0% (w/v) trehalose, 10.0% (w/v) skim milk powder, 0.5% (w/v) ascorbic acid and 0.5% (w/v) gelatin was the best choice for the preservation of P. nigrifaciens. The suggested salinity and concentration of initial cell suspension were 10 g/L NaCl and 1.0 × 109 CFU/mL, respectively. Furthermore, stationary-phase cells were the best choice for the freeze-drying process. The highest survival rate of P. nigrifaciens reached 52.8% when using 5­10% (w/v) skim milk as rehydration medium. Moreover, the other 18 marine strains belonging to Pseudoalteromonas, Vibrio, Photobacterium, Planomicrobium, Edwardsiella, Enterococcus, Bacillus, and Saccharomyces were freezedried under the abovementioned conditions. Their survival rates were 2.3­95.1%. Conclusion: Collectively, our results supported that the protectant mixture and parameters were beneficial for lyophilization of marine bacteria


Subject(s)
Preservation, Biological/methods , Pseudoalteromonas/physiology , Freeze Drying/methods , Trehalose/chemistry , Cell Survival , Bacterial Physiological Phenomena , Disaccharides/chemistry , Microbial Viability , Salinity , Lactose/chemistry , Mannitol/chemistry
2.
Int. j. morphol ; 37(1): 369-374, 2019. tab, graf
Article in Spanish | LILACS | ID: biblio-990053

ABSTRACT

RESUMEN: El auge experimentado en los últimos años en la aplicación de las técnicas anatómicas para la conservación de muestras anatómicas está directamente relacionado con la necesidad de preservación de los escasos especímenes con que cuentan las instituciones universitarias en relación a aumentar el tiempo de utilización del mismo. En este sentido, la plastinación es la técnica anatómica que más se destaca y que permite preservar por tiempo indeterminado, sin toxicidad, las preparaciones anatómicas. Presentamos el protocolo modificado de plastinación a temperatura ambiente con silicona, desarrollado en el Laboratorio de Plastinación y Técnicas Anatómicas de la Universidad de La Frontera, con el objetivo de aplicarla a la conservación de una placenta humana, la cual posteriormente fue pigmentada para otorgarle un aspecto más cercano a lo real.


SUMMARY: The surge experienced in recent years in the application of anatomical techniques for the conservation of anatomical samples is directly related to the need to preserve the few specimens that university institutions have in relation to increase the time of use of the same. In this sense, the plastination is the anatomical technique that stands out and that allows to preserve indefinitely, without toxicity, the anatomical preparations. We present the modified plastination protocol at room temperature with silicone, developed in the Laboratory of Plastination and Anatomical Techniques of the University of La Frontera, with the aim of applying it to the conservation of a human placenta, which was subsequently pigmented to give it an appearance closer to the real.


Subject(s)
Humans , Female , Placenta , Plastination/methods , Preservation, Biological/methods , Silicones/chemistry , Temperature , Tissue Preservation/methods , Acrylic Resins/chemistry , Pigmentation , Plastic Embedding
3.
Braz. j. microbiol ; 49(supl.1): 59-63, 2018. tab, graf
Article in English | LILACS | ID: biblio-1039274

ABSTRACT

Abstract In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection 'University Recife Mycologia' (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.


Subject(s)
Paclitaxel/biosynthesis , Endophytes/metabolism , Fungi/metabolism , Microbiology/organization & administration , Preservation, Biological , Endophytes/genetics , Fungi/genetics
4.
Rio de Janeiro; s.n; 2018. 97 p. ilus.
Thesis in Portuguese | LILACS | ID: biblio-1046368

ABSTRACT

Os métodos de preservação empregados em micologia buscam manter a viabilidade e a estabilidade das características fenotípicas e genéticas. Entretanto, alterações podem ocorrer dependendo da metodologia empregada. Atualmente são escassos os dados da estabilidade dos isolados de Sporothrix spp., incluindo perfis fenotípicos, genotípicos e proteômicos após o armazenamento. Com a recente descrição das novas espécies patogênicas, para o homem e outros mamíferos, torna-se necessário o armazenamento desses fungos em coleções de culturas para estudos futuros, possibilitando maior conhecimento sobre a taxonomia, fisiologia e virulência de Sporothrix spp. Portanto, o objetivo desse estudo foi avaliar métodos de preservação que permitam manter a estabilidade dos perfis fenotípicos, genotípicos e proteômicos dos isolados de Sporothrix spp.. Com esse propósito foram utilizados 8 isolados de Sporotrix spp., armazenados por três diferentes métodos. Preservação em água destilada, sob refrigeração a 4ºC e criopreservação a -80°C, por períodos de 6, 12, 18 e 24 meses de armazenamento. Os isolados foram identificados pelo sequenciamento parcial dos genes calmodulina e ß-tubulina e pela PCR fingerprinting. A estabilidade do fenótipo foi realizada, por meio das provas fenotípicas sugeridas na chave de identificação das espécies de Sporothrix spp. que incluem: morfologia dos conídios, diâmetro da colônia a 30°C, teste de termotolerância a 37°C e assimilação de carboidratos, glicose sacarose e rafinose. O dimorfismo também foi avaliado após a preservação. A estabilidade genotípica foi realizada por meio do sequenciamento parcial do gene codificador da ß-tubulina


A estabilidade proteômica foi realizada por espectrometria de massa (MALDI-TOF MS), após 24 meses de preservação. Todos os isolados foram recuperados dos três métodos em todos os períodos avaliados. No entanto, os isolados preservados em água destilada apresentaram contaminações. Alterações morfológicas foram observadas após preservação a -80°C, um isolado deixou de apresentar colônia pigmentada e conídios demáceos. O diâmetro das colônias assim como a termotolerância permaneceram estáveis nos três métodos. A principal alteração ocorreu com o perfil de assimilação de carboidratos e essa variação modificou a identificação das espécies pela chave taxonômica. A estabilidade genotípica para o gene codificador da ß-tubulina foi preservada após 24 meses em ambos os métodos de armazenamento. Entretanto, baixo polimorfismo foi detectado após preservação em água destilada. Os isolados foram identificados corretamente por espectometria de massa após o armazenamento, porém alterações no perfil protéico foram observadas. O método de preservação a 4°C foi o mais efetivo para preservar os isolados de Sporothrix spp., manteve estável a maioria das características fenotípicas, como também a estabilidade do genótipo por até 24 meses de armazenamento. Esse é um método simples e de baixo custo que pode seu usado em laboratórios de recursos limitados como, um método alternativo de preservação. (AU)


Subject(s)
Animals , Phenotype , Preservation, Biological , Sporothrix , Preservation of Water Samples , Proteomics , Mitosporic Fungi , Genotype
5.
Biosci. j. (Online) ; 33(4): 923-932, july/aug. 2017. ilus, tab
Article in English | LILACS | ID: biblio-966254

ABSTRACT

The preservation methods for fungi have great importance in ex situ collections, representing important biological heritage, useful for mycologists and plant pathologists in several scientific works. However, there is a lack of studies for a suiTable and efficient preservation method for the different groups of fungi. Although, the most appropriate is the one that maintain, even after long periods, the original characteristics of culture: viability, sporulation and pathogenicity, excluding mutations and undesirable contamination. The choice will depend of the laboratory infrastructure, microorganism, objectives, preferences and knowledge of the researcher. We conducted this study inside the Laboratory of Mycology and Plant Protection (LAMIP) in UFU (Universidade Federal de Uberlândia), localized in Uberlândia (MG), Brazil. The objective was to evaluate the gelatin preservation method (17 cultures), never used before for phytopathogenic fungi. Other classical methods were concomitantly evaluated, such as sterile soil (68 cultures), resistant structures (Sclerotinia sclerotiorum) in 4°C (10 strains) and mineral oil (31 cultures). We examined the time for maintaining the viability, sporulation and colonization in host tissues preserved in different dates. The gelatin method remained viability in 10 cultures; this method is suiTable for preservation of the genera and species: Colletotrichum spp., Septoria spp., Fusarium spp., F. moniliforme var. subglutinans, Macrophomina spp., Phomopsis spp. and Verticillium spp. The viability remained in 38 strains of sterile soil, three of mineral oil, and one strain of sclerotia reached a maximum preservation time in 4°C of four years.


Os métodos de preservação de fungos têm grande importância em coleções ex situ, representando importante patrimônio biológico, útil para micologistas e fitopatologista como suporte para vários trabalhos científicos. Não existe um método de preservação adequado e eficiente para os diferentes grupos de fungos, entretanto, o mais apropriado é o que mantém, mesmo após longos períodos, as características originais da cultura: viabilidade, esporulação e patogenicidade, excluindo mutações e contaminação indesejável. A escolha dependerá da infra-estrutura laboratorial, microorganismo, objetivos, preferências e conhecimentos do pesquisador. Este trabalho foi realizado no Laboratório de Micologia e Proteção Vegetal (LAMIP) da UFU (Universidade Federal de Uberlândia), localizado em Uberlândia (MG), Brasil. O objetivo foi avaliar o método de preservação da gelatina (17 culturas), nunca testado antes para fungos fitopatogêncios. Concomitantemente foram avaliados métodos clássicos, como solo estéril (68 culturas), escleródios (Sclerotinia sclerotiorum) em 4 ° C (10 isolados) e óleo mineral (31 culturas). Avaliou-se a manutenção na viabilidade, esporulação e patogenicidade dos isolados. O método da gelatina manteve a viabilidade em 10 culturas, sendo adequado para a preservação dos gêneros e espécies: Colletotrichum spp., Septoria spp., Fusarium spp., F. moniliforme var. subglutinans, Macrophomina spp., Phomopsis spp. e Verticillium spp. A viabilidade foi mantida em 38 isolados em solo estéril, três isolados em óleo mineral e um apenas um isolado de escleródio atingiu um tempo máximo de preservação de quatro anos.


Subject(s)
Preservation, Biological , Ascomycota , Virulence , Fungi
6.
Estud. av ; 31(89): 199-212, 2017.
Article in Portuguese | LILACS | ID: biblio-875240

ABSTRACT

Dados a complexidade socioambiental característica da Região Metropolitana de São Paulo e os problemas e conflitos decorrentes da forma de uso e ocupação do espaço, que gerou novos dilemas, iniquidades e desigualdades sociais, como pensar no enfrentamento de questões complexas e caóticas à luz do paradigma atual de conhecimentos? Como se colocar diante desse cenário de degradação socioambiental? Como pensar no desenvolvimento de políticas públicas ambientalmente efetivas e socialmente justas? Discutiremos aqui uma proposta de governança que promove a conservação ambiental, patrimonial e a sustentabilidade local considerando as relações entre diferentes tipos de saberes, por meio do diálogo e da participação na construção de políticas públicas. Estudos realizados no Geoparque Ciclo do Ouro, em Guarulhos (SP), mostraram serem possíveis alternativas de governabilidade em sistemas complexos diante de dilemas socioambientais urbanos contemporâneos. O Geoparque como proposta de gestão e governança ambiental contribui para preservação e geração de serviços ecossistêmicos com implicações diretas para o lugar e, consequentemente, para o seu entorno.(AU)


Given the environmental complexity characteristic of the city of São Paulo and its environs, the problems and conflicts deriving from use and occupation of space and generating new dilemmas, inequities and inequalities, how does one face the complex and chaotic issues in light of the current knowledge paradigm? How does one place oneself before a scenario of environmental degradation? How does one think about the development of environmentally effective and socially just public policies? This paper discusses a proposal of environmental governance that promotes environmental, heritage conservation and local sustainability by considering the relationships between different types of knowledge through dialogue and participation. Studies carried out at the Ciclo de Ouro Geopark in Guarulhos (SP) show that there are alternatives to the governance of complex systems in face if contemporary urban environmental dilemmas. The Geopark as model for the environmental management and governance contributes to preservation and generation of ecosystem services with direct implications to the site and, consequently, to its surroundings.(AU)


Subject(s)
Humans , Male , Female , Biodegradation, Environmental , Conservation of Natural Resources , Environment , Governance , Public Policy , Socioeconomic Factors , Urbanization , Environmental Management , Preservation, Biological
8.
Bol. micol. (Valparaiso En linea) ; 31(2): 44-50, dic. 2016. ilus, map
Article in English | LILACS | ID: biblio-868815

ABSTRACT

According to the data available at the World Data Center for Microorganism-WDCM from the World Federation for Culture Collection-WFCC, Chile has four registered culture collections that preserve 2777 microbial strains. At the global point of view, the culture collections in Chile are in different level of operation regarding its own infrastructure and compliancy with quality standards for preservation of strains and for services provide. The absence of funding to support the preservation of the Chilean microbial assets is a key issue for the development of the Chilean bioeconomy. Considering this, the Chilean culture collections started working together to establish the Chilean Network of Microbial Culture Collections (RCCCM, acronym in Spanish). In this note, the establishment and operation of the RCCCM is presented and discussed.


De acuerdo con los datos disponibles en el World Data Center for Microorganism-WDCM de la Federación Mundial para la Colección de Cultivos - WFCC, Chile tiene cuatro colecciones de cultivos registradas que preservan 2777 cepas microbianas. Desde el punto de vista global, las colecciones de cultivos en Chile se encuentran en diferentes niveles de operación con respecto a su propia infraestructura y cumplimiento con estándares de calidad para la preservación de las cepas y para los servicios que proporcionan. La ausencia de financiamiento para apoyar la preservación de los activos microbianos chilenos es un tema clave para el desarrollo de la bioeconomía chilena. Considerando esto, las colecciones chilenas de cultivos comenzaron a trabajar conjuntamente para establecer la Red Chilena de Colecciones de Cultivos Microbianos (RCCCM). En esta nota se presenta y discute el establecimiento y funcionamiento de la RCCCM.


Subject(s)
Economics , Microbiology/economics , Microbiology/organization & administration , Preservation, Biological , Chile
9.
Braz. j. microbiol ; 47(2): 403-409, Apr.-June 2016. tab, graf
Article in English | LILACS | ID: lil-780836

ABSTRACT

Abstract Considering the absence of standards for culture collections and more specifically for biological resource centers in the world, in addition to the absence of certified biological material in Brazil, this study aimed to evaluate a Fungal Collection from Fiocruz, as a producer of certified reference material and as Biological Resource Center (BRC). For this evaluation, a checklist based on the requirements of ABNT ISO GUIA34:2012 correlated with the ABNT NBR ISO/IEC17025:2005, was designed and applied. Complementing the implementation of the checklist, an internal audit was performed. An evaluation of this Collection as a BRC was also conducted following the requirements of the NIT-DICLA-061, the Brazilian internal standard from Inmetro, based on ABNT NBR ISO/IEC 17025:2005, ABNT ISO GUIA 34:2012 and OECD Best Practice Guidelines for BRCs. This was the first time that the NIT DICLA-061 was applied in a culture collection during an internal audit. The assessments enabled the proposal for the adequacy of this Collection to assure the implementation of the management system for their future accreditation by Inmetro as a certified reference material producer as well as its future accreditation as a Biological Resource Center according to the NIT-DICLA-061.


Subject(s)
Preservation, Biological/standards , Fungi/classification , Mycology/organization & administration , Quality Control , Brazil , Fungi/isolation & purification , Fungi/genetics , Mycology/standards
10.
Acta sci., Biol. sci ; 38(2): 187-194, abr.-jun. 2016.
Article in English | LILACS | ID: biblio-2532

ABSTRACT

The River Piancó in the municipality of Pombal on the western stretch of the state of Paraíba, Brazil, receives contaminated effluents. Current assay evaluates the microbiological quality of the river water and analyzes the social and environmental aspects of the riverine community with regard to the water and its use of the river. The water´s microbiological quality was verified at four sites on the river in five two- monthly collections throughout the year (totaling 20 samples) for the following analyses: Most Probably Number (MPN), Total (TC) and Fecal Coliform (FC) and identification of Escherichia coli. The social and environmental aspects were assessed by a 15-question questionnaire given to thirty homes. Results report Fecal Coliforms (> 240 to > 1,100 MPN 100 mL-1), Total (> 93 to > 1,100 MPN 100 mL-1) and high concentrations of E. coli (> 898 to > 1,100 UFC 100 mL-1) in all the sampling sites. The river is frequented by the riverine people for home consumption, recreation and fishing. Conditions of the River Piancó, especially in the stretch that margins the town of Pombal, are highly critical and exceed the limits of current Brazilian legislation with regard to drinking and swimming conditions.


O Rio Piancó, na sua porção oeste do Estado da Paraíba, no município de Pombal, recebe efluentes que têm causado a sua contaminação. Este trabalho objetivou avaliar a qualidade microbiológica deste rio e analisar aspectos socioambientais da comunidade ribeirinha sobre a água e o uso do rio. A qualidade microbiológica foi verificada em quatro pontos do rio, em cinco coletas bimestrais ao longo do ano (20 amostras no total), para análises do Número Mais Provável (NMP), Coliformes Totais (CT) e Fecais (CF) e identificação da Escherichia coli. A avaliação socioambiental foi por meio da aplicação de um questionário socioambiental constituído por 15 perguntas, aplicados a uma população de 30 residências. Os resultados reportaram a presença de Coliformes Fecal (> 240 a > 1.100 NMP 100 mL-1), Total (> 93 a > 1.100 NMP 100 mL-1) e altas concentrações de E. coli (> 898 a > 1.100 UFC 100 mL-1) em todos os pontos amostrais. O rio é utilizado pelos moradores ribeirinhos, principalmente, para o consumo doméstico, a recreação primária e a pesca. A atual situação do Rio Piancó, na porção em que margeia a cidade de Pombal é crítica, ultrapassando todos os limites da legislação brasileira vigente quanto à potabilidade e balneabilidade da água do rio.


Subject(s)
Humans , Environmental Microbiology , Colimetry , Environmental Pollution , Perception , Preservation, Biological , Water Resources
11.
Pesqui. vet. bras ; 36(2): 131-135, fev. 2016. tab, graf
Article in English | LILACS | ID: lil-777394

ABSTRACT

The present paper reports blood gas analysis, hematologic and micro bacteriologic information on four female Red-billed curassows, rescued from illegal wild animal trading, that were undergoing habilitation for reintroduction into the wild through Projeto Centrofauna/Botucatu-SP. There is a lack of physiological data on this species, endemic to the region of Atlantic Forest (Mata Atlântica), now under threat of extinction. This lack of information makes the clinical evaluation of these birds very difficult and has a direct and adverse effect on any of these birds received for medical treatment in hospitals, veterinary clinics and centers for research and conservation, as well as in reintroduction centers.


O presente artigo reporta dados inéditos de análise hemogasométrica, hematológica e bacteriológica de quatro fêmeas de Mutum do Sudeste, resgatadas do tráfico ilegal de animais silvestres e em processo de habilitação e reintrodução para vida-livre pelo Projeto Centrofauna, Botucatu/SP. Na literatura há uma falta de dados fisiológicos sobre esta espécie, endêmica da região da Mata Atlântica e ameaçada de extinção. Esta falta de informação faz com que avaliações clínicas nesta espécie aviária sejam muito difíceis de interpretar, causando um efeito direto e negativo nestas aves para a realização de tratamento médico em hospitais, clínicas veterinárias, centros de pesquisa e/ou conservação, bem como centros de reintrodução.


Subject(s)
Animals , Female , Birds , Escherichia coli , Microbiota , Bacteriological Techniques/analysis , Preservation, Biological/veterinary , Hematologic Tests/veterinary
12.
Pesqui. vet. bras ; 36(1): 62-66, Jan. 2016. graf
Article in English | LILACS | ID: lil-777382

ABSTRACT

Currently the importance of using alternative strategies for biodiversity conservation is emphasized and since the establishment of germplasm bank is an alternative to the conservation of endangered species. This is a technique of great importance for the maintenance of Brazilian fauna. Since the early70'sthere was a growing concern about the need to preserve essential genetic resources for food and agriculture, mainly for conservation of genetic material from farm animals. Thus was created the Brasilia Zoo, in July 2010, the first Germplasm Bank of Wild Animals in Latin America, as an alternative strategy for the conservation of threatened or endangered species, using both gametes and somatic cells and stem cells. Then we argue to create new banks or research networks among different regions with aimed to tissue preservation.


Atualmente, a importância do uso de estratégias alternativas para a preservação da biodiversidade é ressaltada e, visto que a criação de bancos de germoplasma é uma alternativa para a conservação de espécies ameaçadas, esta é uma técnica de suma importância para a manutenção da fauna brasileira. Desde o começo da década de 70 houve uma crescente preocupação sobre a necessidade de se preservar recursos genéticos essenciais para alimentação e agricultura, voltados principalmente, para a conservação de material genético de animais de produção. Deste modo, foi criado pelo Jardim Zoológico de Brasília, em julho de 2010, o primeiro Banco de Germoplasma de Animais Selvagens da América Latina, como uma estratégia alternativa para a conservação de espécies ameaçadas ou em perigo de extinção, utilizando tanto gametas como células somáticas e células-tronco. Com isto ponderamos na criação de novos bancos ou redes de pesquisa inter-regionais que foquem nesta preservação tecidual.


Subject(s)
Animals , Biodiversity , Cryopreservation/veterinary , Endangered Species , Preservation, Biological/veterinary , Tissue Preservation/veterinary
13.
Rev. MVZ Córdoba ; 21(1): 5124-5136, Jan.-Apr. 2016. ilus, tab
Article in English | LILACS, COLNAL | ID: lil-797425

ABSTRACT

Objective. This study is a preliminary step of a larger national program aimed to develop a strategy for "in situ" preservation of Teleorman Black Head sheep population. In this paper we estimated the effect of β-lactoglobulin, casein and prolactin on some quantitative and qualitative milk traits in this local sheep population. Material and methods. Genotyping methodology included PCR for CSN3 (A and B alleles) and PCR-RFLP for LGB (A and B alleles) and PRL (T and C alleles), respectively. Repeated milking and milk composition analysis were used for the polymorphism effect estimation. Results. No association between CSN3 polymorphism and milk traits was found. Effect of LGB on production traits was quite constant. Genotype AA performed better than AA. PRL marker effect showed small differences than LGB. Concerning milk, fat and protein yield, AA genotype for PRL had a smaller positive impact than AA genotype for LGB. Regarding fat and protein content, PRL showed a negative effect for AA and positive for AA genotype, respectively. Conclusions. Positive association between LGB and milk yield and composition recommend this candidate gene like marker for a future MAS program. Although PRL gene is also associated with an increased milk quantity, inverse response over milk composition must be considered in MAS strategy. Our study demonstrated that both LGB and PRL markers could became an advent of MAS utilization in Romanian dairy sheep breeding industry.


Objetivo. Este estudio es un paso preliminar de un programa nacional más amplio destinado a desarrollar una estrategia para la conservación "in situ"de la población de ovejas Cabeza Negra de Teleorman. En este trabajo se estimó el efecto de la β-lactoglobulina, caseína y prolactina en algunos rasgos cuantitativos y cualitativos de la leche en esta población de ovejas locales. Material y métodos. Metodología de PCR para genotipificación incluido CSN3 (A y B alelos) y PCR-RFLP para LGB (A y B alelos) y PRL (T y C alelos). Análisis y composición de la leche de ordeños repetidos se utilizaron para estimación el efecto del polimorfismo. Resultados. No se encontró asociación entre el polimorfismo y la leche rasgos CSN3. Efecto de LGB en los rasgos de producción era bastante constante. Genotipo AA obtenido mejores resultados que AA. Efecto marcador PRL mostró pequeñas diferencias que LGB. En cuanto a la leche grasa y proteína el genotipo AA para PRL tuvo un impacto positivo más pequeño que el genotipo AA para LGB. En cuanto a contenido de grasa y proteína, PRL mostró un efecto negativo para AA y positivo para AA genotipo. Conclusiones. La asociación positiva entre LGB y la producción de leche y la composición recomienda este gen candidato como marcador para un futuro programa de MAS. Aunque gen PRL también se asocia con un incremento en la cantidad de leche, la respuesta inversa sobre composición de la leche debe ser considerado en la estrategia de MAS. Nuestro estudio demostró que los marcadores tanto LGB y PRL podrían venir a ser utilizados en MAS en la industria rumana de cría de ovejas lecheras.


Subject(s)
Biodiversity , Genes , Preservation, Biological
14.
Rev. MVZ Córdoba ; 20(supl.1): 5028-5036, Dec. 2015. ilus, tab
Article in English | LILACS, COLNAL | ID: lil-769259

ABSTRACT

Objective. Growth and mortality parameters of Cachana Cynopotamus atratoensis Eigenmann, 1907 in the cienaga Grande de Lorica, Sinu river basin, Colombia, were estimated. Materials and methods. The length frequency analysis to the information collected between January 2001 to December 2002, was applied. Results. The estimated values for asymptotic length, growth rate and age at zero length were 43.4 (±0.52) cm of total length, 0.29 (±0.02) year-1 and -0.53 years, respectively. Total mortality was estimated at 0.97 year-1, natural mortality at 0.72 year-1, fishing mortality at 0.25, the average catch size was 24.8 cm total length, being fully recruited to the fishery with 16.6 cm total length, with low exploitation rate (E: 0.26). Conclusions. Cachana is a fish with medium longevity and growth rate with low exploitation rate, suggesting that there is still no overfishing on the species. However, as it is an very important fish in the food security of fishermen and their families, its necessary to increase the basic biology research pointing to the management and conservation of the species in the cienaga and the Sinu basin.


Objetivos. Estimar los parámetros de crecimiento y mortalidad de la Cachana Cynopotamus atratoensis Eigenmann, 1907 en la ciénaga Grande de Lorica, cuenca del río Sinú, Colombia. Materiales y métodos. Se aplicó el análisis de frecuencia de tallas a la información colectada en el período comprendido entre enero 2001 y diciembre 2002. Resultados. Los valores estimados para la longitud asintótica, tasa de crecimiento y edad a la longitud cero fueron 43.4 (±0.52) cm de longitud total, 0.29 (±0.02) año-1 y -0.53 años, respectivamente. La mortalidad total fue estimada en 0.97 año-1, la mortalidad natural en 0.72 año-1, la mortalidad por pesca en 0.25 año-1, la talla media de captura fue 24.8 cm de longitud total, siendo reclutada totalmente a la pesquería con 16.6 cm de longitud total, con baja tasa de explotación (E=0.26). Conclusiones. La Cachana es un pez de longevidad y tasa de crecimiento medios con baja tasa de explotación, lo que sugiere que aún no hay sobrepesca sobre la especie. Sin embargo, como es un pez muy importante en la seguridad alimentaria de los pescadores y sus familias, es necesario incrementar la investigación sobre su biología básica apuntando al manejo y conservación de la especie en la ciénaga y la cuenca del Sinú.


Subject(s)
Preservation, Biological , Wetlands
15.
Article in Chinese | WPRIM | ID: wpr-237740

ABSTRACT

During the process of growth, harvesting, transportation, processing and storage, Chinese herbal medicines (CHMs) can be easily contaminated by fungi and their metabolites like mycotoxins, which not only express negative effects on the quality and safety of CHMs and their processed products, but also pose great threats to human health. Now, some chemical synthetic fungicides have been frequently used to control the growth of fungi and accumulation of mycotoxins in the preservation of CHMs. However, the concentration and type of chemical fungicides allowed for postharvest application are restricted due to the disadvantages of their high residual toxicity, long degradation period and pollution to the environment and so on. Therefore, it is critical to research and develop some highly effective, safe and non-toxic, natural, environment-friendly fungistatic agents from plants to prevent CHMs from being contaminated by fungi and mycotoxins. The paper reviews mycotoxins and their harmfulness, the effective compounds of fungistatic plants as well as the antifungal mechanism to provide scientific evidences for developing novel and effective fungistatic agents plants. Then, the application prospect of fungistatic agents from plants in the preservation of CHMs was discussed.


Subject(s)
Animals , Fungi , Metabolism , Fungicides, Industrial , Pharmacology , Humans , Mycotoxins , Metabolism , Toxicity , Plant Diseases , Microbiology , Plant Extracts , Pharmacology , Plants, Medicinal , Chemistry , Microbiology , Preservation, Biological , Methods
17.
Braz. j. microbiol ; 45(1): 43-47, 2014. tab
Article in English | LILACS | ID: lil-709477

ABSTRACT

Microorganisms are a source of many high-value compounds which are useful to every living being, such as humans, plants and animals. Since the process of isolating and improving a microorganism can be lengthy and expensive, preserving the obtained characteristic is of paramount importance, so the process does not need to be repeated. Fungi are eukaryotic, achlorophyllous, heterotrophic organisms, usually filamentous, absorb their food, can be either macro or microscopic, propagate themselves by means of spores and store glycogen as a source of storage. Fungi, while infesting food, may produce toxic substances such as mycotoxins. The great genetic diversity of the Kingdom Fungi renders the preservation of fungal cultures for many years relevant. Several international reference mycological culture collections are maintained in many countries. The methodologies that are most fit for preserving microorganisms for extended periods are based on lowering the metabolism until it reaches a stage of artificial dormancy . The goal of this study was to analyze three methods for potentially toxigenic fungal conservation (Castellani's, continuous subculture and lyophilization) and to identify the best among them.


Subject(s)
Fungi/isolation & purification , Fungi/physiology , Microbiological Techniques/methods , Preservation, Biological/methods
18.
Chinese Journal of Biotechnology ; (12): 1128-1133, 2014.
Article in Chinese | WPRIM | ID: wpr-279441

ABSTRACT

To compare two enrichment and preservation methods of urinary proteins, stored in polyvinylidene difluoride (PVDF) membrane (Urimem) or direct freezing, we examined the differences between the two methods in time, space, costs of supplies and electricity, degree of protein degradation and convenience of the sample handling. The urimem method is superior in the storage space, the cost of electricity and the clinical convenience compared to the direct freezing method. However, the direct freezing method is superior in the time and the cost of supplies to the urimem method. The enrichment and preservation of urinary proteins using urimem have more cost-effective benefits compared to those of the direct freezing method.


Subject(s)
Cost-Benefit Analysis , Freezing , Humans , Polyvinyls , Preservation, Biological , Methods , Proteins , Chemistry , Urine , Chemistry
20.
Rev. Inst. Med. Trop. Säo Paulo ; 55(1): 7-11, Jan.-Feb. 2013. ilus, tab
Article in English | LILACS | ID: lil-661096

ABSTRACT

Coccidioidomycosis is an emerging fungal disease in Brazil; adequate maintenance and authentication of Coccidioides isolates are essential for research into genetic diversity of the environmental organisms, as well as for understanding the human disease. Seventeen Coccidioides isolates maintained under mineral oil since 1975 in the Instituto de Medicina Tropical de São Paulo (IMTSP) culture collection, Brazil, were evaluated with respect to their viability, morphological characteristics and genetic features in order to authenticate these fungal cultures. Only five isolates were viable after almost 30 years, showing typical morphological characteristics, and sequencing analysis using Coi-F and Coi-R primers revealed 99% identity with Coccidioides genera. These five isolates were then preserved in liquid nitrogen and sterile water, and remained viable after two years of storage under these conditions, maintaining the same features.


Coccidioidomicose é uma doença emergente no Brasil; a manutenção adequada e autenticação de isolados de Coccidioides spp são essenciais para a pesquisa em diversidade genética de micro-organismos, bem como para a compreensão da doença em humanos. Dezessete isolados de Coccidioides preservados em óleo mineral desde 1975 na coleção de culturas do Instituto de Medicina Tropical de São Paulo (IMTSP) foram avaliados com relação à viabilidade, características morfológicas e genéticas, com o objetivo de autenticação das culturas fúngicas. Dos 17 isolados, apenas cinco foram viáveis após quase 30 anos mantidos em óleo mineral, apresentando características morfológicas e moleculares típicas do gênero, o sequenciamento utilizando os oligonucleotídeos Coi-F e Coi-R revelou identidade de 99% com isolados de Coccidioides. Estes cinco isolados foram preservados em nitrogênio líquido e água destilada esterilizada, e permaneceram viáveis após dois anos de armazenamento sob estas condições, mantendo as mesmas características.


Subject(s)
Humans , Coccidioides/physiology , Microbial Viability , Preservation, Biological/methods , Brazil , Coccidioides/genetics , Genotype , Mineral Oil , Phenotype , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL