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1.
Rev. bras. ciênc. vet ; 28(2): 97-101, abr./jun. 2021. il.
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1367729

ABSTRACT

Objetivou-se investigar a presença do Vírus da Estomatite Vesicular (VEV) e seus fatores de risco para ocorrência e disseminação da enfermidade em equídeos das mesorregiões Leste e Oeste Potiguar do estado do Rio Grande do Norte, Brasil. Foram analisadas pela técnica de virusneutralização, 809 amostras sanguíneas de equídeos provenientes de noventa propriedades de dezesseis municípios Potiguares durante os meses de julho de 2018 a fevereiro de 2019. Os fatores de riscos associados ao VEV foram avaliados por meio de questionário epidemiológico e os dados submetidos a análise estatística no programa IBM SPSS Statistics versão 21.0 com nível de confiança de 95%. Posteriormente, todas as variáveis estatisticamente significantes foram submetidas a análise de regressão de Poisson. A soroprevalência de anticorpos anti-VEV foi 24,6% (199/809), sendo 3,2% (13/402) de soropositivos na mesorregião Leste e 45,7% (186/407) na do Oeste Potiguar. Com relação aos sorotipos, observou-se uma prevalência de 3,8% (31/809) e 24,5% (198/809) para Indiana 2 e 3 respectivamente, com 15,1% (30/198) de coinfecção. Equídeos criados na mesorregião Oeste, em propriedades que não realizam quarentena e onde os animais enfermos são mantidos no rebanho, foram consideradas fatores predisponentes a infecção pelo VEV. Esses resultados demonstram a circulação do VEV em equídeos no Rio Grande do Norte, com destaque ao Oeste Potiguar, e sendo necessário a aplicação de medidas sanitárias que impeçam introdução e disseminação do vírus ente as espécies susceptíveis, principalmente em condições climáticas favoráveis para a sua manutenção, no ambiente de criação e pastagens.


This study aimed to investigate the presence of Vesicular stomatitis virus (VSV) and risk factors for its occurrence and dissemination in equines from the Eastern and Western mesoregions of the state of Rio Grande do Norte, Brazil. Blood samples were analyzed, by Serum Virus Neutralization Assay, from 809 animals belonging to 90 properties distributed in sixteen municipalities from July 2018 to February 2019. Risk factors were assessed using an epidemiological questionnaire. Data were submitted to statistical analysis using the software IBM SPSS Statistics, version 21.0 with a 95% confidence level. Also, all statistically significant variables were subjected to Poisson regression analysis. The occurrence of anti-VSV antibodies was 24.6% (199/809) with 3.2% (13/402) and 45.7% (186/407) of seropositivity in the Western and Eastern mesoregion, respectively. Regarding serotypes, there were an occurrence of 3.8% (31/809) and 24.5% (198/809) for Indiana 2 and 3, respectively, and 15.1% (30/198) of co-infection for both. Equines kept of the Western mesoregion, on properties that do not quarantine, and where sick animals are kept in the herd, were considered risk factors for LVV infection. These results demonstrate the presence of VSV in equines in Rio Grande do Norte, with emphasis on Oeste Potiguar, and that sanitary measures must be adopted to prevent the introduction and viral spreading among susceptible species, especially due to favorable climatic conditions for the maintenance of VSV in the breeding and pasture environment.


Subject(s)
Animals , Vesicular stomatitis Indiana virus , Horse Diseases/virology , Risk Factors , Vesicular Stomatitis/virology , Antibodies, Viral/analysis
2.
Pesqui. vet. bras ; 35(5): 391-395, May 2015. tab
Article in Portuguese | LILACS | ID: lil-759376

ABSTRACT

A Estomatite Vesicular (EV) é uma doença infecciosa que acomete equinos, bovinos, suínos, mamíferos silvestres e humanos. Por apresentar sinais clínicos semelhantes a outras doenças vesiculares, principalmente, febre aftosa, sua presença em determinadas regiões pode interferir no intercâmbio comercial internacional dos animais, seus produtos e subprodutos. Apesar de sua importância, a epidemiologia e a manutenção do vírus no ambiente não estão totalmente esclarecidas dificultando a aplicação de medidas de controle efetivas. A doença já foi diagnosticada em todas as regiões brasileiras. Bovinos com sialorréia, perda do epitélio lingual, lesões abertas com bordas amareladas nas gengivas, lábios, língua e mucosa oral e equinos com sialorréia e lesões abertas na mucosa oral e lábios foram observados e notificados ao Serviço Veterinário Oficial do Estado do Maranhão, Agência Estadual de Defesa Agropecuária do Maranhão (AGRD/MA). Amostras de soro de equinos e bovinos com sintomas de EV foram coletadas para investigação por ELISA e por neutralização viral, além do diagnóstico diferencial para Febre Aftosa (FA). Fragmentos epiteliais de bovinos com lesões na língua foram coletados para identificação molecular do agente. Todos os animais foram negativos para FA. Todos os bovinos e equinos foram reativos para EV nos testes sorológicos. A partir dos fragmentos epiteliais de bovinos enviados ao Instituto Biológico de São Paulo para PCR, foi possível caracterizar o agente como VesiculovirusIndiana III (Alagoas/VSAV).


Vesicular stomatitis (VS) is an infectious viral disease that affects bovines, equines, swine, wild animals and humans. As it is indistinguishable from other vesicular diseases, mainly Foot and Mouth Disease (FMD), it causes restrictions in commercial livestock trade at national and international levels and also significant economic losses. As the epidemiology and maintenance of VS virus in nature are not clearly understood it is difficult to take effective control measures. VS was diagnosed in some regions of Brazil, such as Minas Gerais, Santa Catarina, São Paulo and Alagoas. Cattle and horses with clinical symptoms of drooling, shedding of the lingual epithelium, presence of vesicles on the oral mucosa were observed and reported to the National Animal Health Office health of Maranhão State, Brazil. Samples of serum of these animals were collected and sent to Laboratório Nacional de Agropecuaria for ELISA and virus neutralization and differential diagnosis for Foot and Mouth Disease (FMD). The results of ELISA confirmed the VS. In the differential diagnosis, the results were negative for FMD. Samples of bovine epithelial tissue for VS by PCR confirmation of diagnosis were collected and sent to Biological Institute of São Paulo. Molecular results confirmed the VesiculovirusIndiana III (Alagoas/VSAV) infection.


Subject(s)
Animals , Cattle , Vesicular Stomatitis/diagnosis , Vesicular Stomatitis/epidemiology , Vesicular Stomatitis/prevention & control , Vesicular Stomatitis/virology , Epidemiological Monitoring/veterinary , Disease Notification , Disinfection , Quarantine/veterinary , Polymerase Chain Reaction/veterinary , Disease Outbreaks/veterinary , Vector Control of Diseases , Vesicular stomatitis Indiana virus , Vesicular stomatitis New Jersey virus
3.
Chinese Journal of Virology ; (6): 548-553, 2012.
Article in Chinese | WPRIM | ID: wpr-340007

ABSTRACT

To clone porcine bone marrow stromal antigen-2 (BST-2) gene, construct its recombinant eukaryotic expression plasmid and induce the expression of the fusion antiviral protein, we amplified BST-2 gene by RT-PCR from the total RNA extracted from PK15 cells. The recombinant expression plasmid pcDNA-BST-2 was constructed and then was transfected into HEK293T cells to expresse the BST-2 fusion protein. Western blot and indirect immunofluorescence assay (IFA) were performed, and the biological activity was detected. The results showed that the construction of recombinant plasmid pcDNA-BST-2 was confirmed by restriction enzyme digestion and sequencing. The expressed product had antiviral activity against Vesicular stomatitis virus (VSV), Avian influenza virus (AIV) and Porcine reproductive and respiratory syndrome virus (PRRSV). In conclusion, the research paves the way for further research on bioactivity assayand antiviral medication.


Subject(s)
Animals , Antigens, CD , Genetics , Allergy and Immunology , Cell Line , Chickens , Cloning, Molecular , Gene Expression , Humans , Influenza in Birds , Allergy and Immunology , Virology , Orthomyxoviridae , Physiology , Porcine Reproductive and Respiratory Syndrome , Allergy and Immunology , Virology , Porcine respiratory and reproductive syndrome virus , Physiology , Swine , Vesicular Stomatitis , Allergy and Immunology , Virology , Vesicular stomatitis Indiana virus , Physiology , Virus Replication
4.
Chinese Journal of Cancer ; (12): 805-814, 2011.
Article in English | WPRIM | ID: wpr-294456

ABSTRACT

In the last decade, we have gained significant understanding of the mechanism by which vesicular stomatitis virus (VSV) specifically kills cancer cells. Dysregulation of translation and defective innate immunity are both thought to contribute to VSV oncolysis. Safety and efficacy are important objectives to consider in evaluating VSV as a therapy for malignant disease. Ongoing efforts may enable VSV virotherapy to be considered in the near future to treat drug-resistant ovarian cancer when other options have been exhausted. In this article, we review the development of VSV as a potential therapeutic approach for recurrent or drug-resistant ovarian cancer.


Subject(s)
Animals , Antineoplastic Agents , Pharmacology , Apoptosis , Cell Proliferation , Drug Resistance, Neoplasm , Female , Humans , Neoplasm Recurrence, Local , Oncolytic Virotherapy , Methods , Ovarian Neoplasms , Pathology , Therapeutics , Virology , Vesicular stomatitis Indiana virus , Physiology , Virus Replication
5.
Chinese Journal of Biotechnology ; (12): 1258-1262, 2008.
Article in Chinese | WPRIM | ID: wpr-275393

ABSTRACT

Equine interferon-gamma (eIFN-gamma) expressed both in E. coli and baculovirus were evaluated for antiviral activity against recombinant Vesicular Stomatits Virus expressing green fluorescence protein (rVSV-GFP) in EFK-78 cells. The assays were conducted in 96-well plate. Virus infectivity was measured by quantifying GFP-positive cells, instead of quantifying the CPE reduction. Prior to infection of EFK-78 cells with rVSV-GFP, the cells were incubated with eIFN-gamma. The GFP expression in the EFK-78 cells dramatically decreased in the cells treated with eIFN-gamma in a dose-dependent manner, comparing with the mock-treated cells. The titers of antiviral activity were 1 x 10(3) AU/mL and 1 x 10(5) AU/mL of eIFN-gamma expressed from E. coli and baculovirus, respectively. The antiviral activities of the recombinant eIFN-gamma were highly efficient and specific, as it was blocked by mAbs against eIFN-gamma.


Subject(s)
Animals , Antibodies, Monoclonal , Allergy and Immunology , Antiviral Agents , Metabolism , Pharmacology , Baculoviridae , Genetics , Metabolism , Escherichia coli , Genetics , Metabolism , Green Fluorescent Proteins , Metabolism , Horses , Interferon-gamma , Genetics , Pharmacology , Recombinant Proteins , Vesicular stomatitis Indiana virus , Metabolism
6.
Braz. j. microbiol ; 38(3): 500-506, July-Sept. 2007. ilus, tab
Article in English | LILACS | ID: lil-464779

ABSTRACT

Vesicular Stomatitis (VS) is a viral disease that has a great impact in animal health, as infected animals present marked decrease in meat and milk production. Its presence is a limiting factor for international animal trade. Besides the damage in the livestock productivity, such disease assumes an important role in animal health programs since it is clinically indistinguishable from Foot-and-Mouth Disease. The diagnosis of the VS has been made, mainly, through Complement Fixation, ELISA and Virus Neutralization tests, assays that allow not only for viral detection but also for differentiation of the two serotypes described for Vesicular Stomatitis Virus (VSV): New Jersey (NJ) and Indiana (Ind). In this work, a molecular diagnostic approach, the polymerase chain reaction performed after reverse transcription (RT - PCR), based on the specific partial amplification of NS gene of VSV was used, as an alternative method for the detection of the virus. A total of 10 VSV reference samples and 12 specimens collected from animals with clinical signs of vesicular disease obtained from field episodes in Ecuador were tested. The method allowed for the specific partial amplification of the region coding for protein P, both for VSV serotypes New Jersey (642 bp) and Indiana 1 (614 bp). The results were compatible with data obtained by Complement Fixation test and the identity of the amplified products was confirmed by nucleotide sequencing.


A Estomatite Vesicular (EV) é uma enfermidade viral de grande impacto na saúde animal. O animal enfermo apresenta queda na produtividade em rebanho de carne e na produção leiteira, sendo um fator limitante para o comércio internacional de animais. Além dos danos à produtividade essa enfermidade assume importante papel nos programas de saúde animal por ser indistinguível clinicamente da Febre Aftosa. As técnicas para o diagnóstico da EV são, principalmente, a Fixação de Complemento, a ELISA e a Virusneutralização, testes que permitem a detecção viral e a diferenciação dos dois sorotipos descritos para o vírus da Estomatite Vesicular (VEV): New Jersey (NJ) e Indiana (Ind). Neste trabalho a metodologia molecular da reação em cadeia da polimerase após transcrição reversa (RT - PCR) baseada na amplificação parcial específica do gene NS do VEV foi utilizada como um método alternativo para a detecção do vírus. Um total de 10 amostras de referência do VEV e 12 espécimes coletados de animais com sinais clínicos de enfermidade vesicular obtidas de episódios de campo em Equador foi testado. O método permitiu a amplificação parcial da região que codifica para proteína P, tanto para NJ (642 pb) quanto para Ind (614 pb). Os resultados foram concordantes com os dados obtidos por Fixação de Complemento e a identidade dos produtos amplificados foi confirmada por meio de seqüenciamento nucleotídico.


Subject(s)
Cattle , In Vitro Techniques , Complement System Proteins , Stomatitis , Diagnostic Techniques and Procedures , Vesicular stomatitis Indiana virus/isolation & purification , Enzyme-Linked Immunosorbent Assay , Methods , Polymerase Chain Reaction
7.
Journal of Biomedical Engineering ; (6): 1111-1117, 2007.
Article in Chinese | WPRIM | ID: wpr-345999

ABSTRACT

Transgenic chicken and oviduct bioreactor are growing to be one of the hotspot of scientific study in the field of biology. The most successful method of producing transgenic chicken is pseudotyped retrovirus vector system, but no one has reported the production of transgenic chicken by retrovirus system recently in our country. In order to accelerate our study in this field, we introduced the relevant technical methods such as packaging retrovirus and vesicular stomatitis virus G glycoprotein (VSV-G) pseudotyped retrovirus, optimizing the conditions of packaging retrovirus, concentrating VSV-G pseudotyped retrovirus, helper virus assays, and microinjection of retrovirus. Furthermore, we successfully conducted in vivo study for detecting the marker gene EGFP of chicken embryo as well as in vitro study for detecting that gene of chicken embryo myoblast (CFM), thus we have provided an applied technical platform for studies of transgenic chicken in the future.


Subject(s)
Animals , Animals, Genetically Modified , Chick Embryo , Chickens , Genetics , DNA Primers , Genetic Vectors , Genetics , Retroviridae , Genetics , Vesicular stomatitis Indiana virus , Genetics
8.
Article in Chinese | WPRIM | ID: wpr-280658

ABSTRACT

To determine whether addition of vitamin C (Vit C) to single-unit plasma could influence the efficacy of inactivating viruses and could maintain the activity of plasma proteins by methylene blue (MB)-light treatment. Vesicular stomatitis virus (VSV) Indiana strain was used as the indicating virus. Human plasma containing VSV was added with different concentrations of Vit C and final concentration 1 micromol/L MB and irradiated by fluorescence at an intensity of 40,000 lx, samples were collected at different times for detection. Cytopathic effect was used to test the effect of virus inactivation. A segment of the nucleic acid encoding capsid protein of VSV was amplified with RT-PCR. Some methods, such as the Clauss method, the one-stage method, microimmunoelectrophoresis, were used to investigate the changes of plasma components. The results showed that when the VSV plasma was added with 240 micromol/L Vit C and treated by MB-light irradiation for 60 min, the titer of VSV decreased by more than 8 lg TICD50/ml. Meanwhile, target segment amplification of VSV was also negative. The recovery rates of fibrinogen and coagulation factor VIII (FVIII: C) were 83.55% and 81.67% respectively, which had significant difference comparing with the routine MB-fluorescent light treatment. Most of plasma proteins were not affected significantly. No change in immunogenicity of these proteins was observed by using microimmunoelectrophoresis. It is concluded that virus inactivation is not influenced and plasma proteins are effectively protected by Vit C. Vit C can be used as a protector and is beneficial to improving the quality of plasma subjected to MB- photodynamic treatment.


Subject(s)
Ascorbic Acid , Pharmacology , Blood Proteins , Metabolism , Humans , Light , Methylene Blue , Pharmacology , Plasma , Virology , Vesicular stomatitis Indiana virus , Virus Inactivation
9.
Rev. colomb. cienc. pecu ; 18(2): 115-121, ago.2005. tab, graf
Article in Spanish | LILACS | ID: lil-463014

ABSTRACT

Con el objetivo de determinar la respuesta inmune humoral en porcinos inducida por una vacuna bovina comercial contra la estomatitis vesicular, se inmunizaron 30 cerdas comerciales y 20 más fueron dejadas como control. A todas se les realizó medición de los títulos de anticuerpos utilizando la prueba de seroneutralización para los serotipos Indiana (IN) y New Jersey (NJ), los días 0, 82, 182, 330, 404 y 599 post-vacunación; con revacunación a los 434 días. Tanto los animales vacunados como los no vacunados exhibieron bajos títulos de anticuerpos contra ambos serotipos antes de la vacunación. Sin embargo al día 82 post-vacunación se presentó un notable incremento en los títulos de anticuerpo en animales vacunados tanto para el serotipo IN como para el NJ, con promedios de 3.17 y 3.56 respectivamente. Para el día 404 se observó un descenso en el título de anticuerpos, los cuales incrementan para el día 599, por efecto de la revacunación. Los animales control mantuvieron bajos títulos de anticuerpos durante todo el experimento. Este estudio muestra que hay una respuesta inmunehumoral con diferencias estadísticamente significativas entre las cerdas vacunadas y no vacunadas, sin reacciones adversas atribuibles al biológico, en los animales inoculados.


Subject(s)
Animals , Antibody Formation , Swine , Vaccines , Vesicular stomatitis Indiana virus
10.
Rev. argent. microbiol ; 36(3): 136-138, jul.-sep. 2004. ilus, tab
Article in English | LILACS | ID: lil-634471

ABSTRACT

Different immunomodulatory activities present in Trichilia glabra (TG) leaf extracts have already been described. Particularly, chloroform-methanol extracts were responsible for an in-vivo anti-inflammatory effect. The effect of such extracts on the infectivity of enveloped and naked viruses were investigated. Methanolic fraction extracts were active against herpes simplex virus type 1 (HSV-1) and vesicular stomatitis virus (VSV), while no activity against poliovirus type 3 was observed. VSV was slightly more affected than HSV-1: 2.8 log10 reduction in VSV titer against 2.4 log10reduction in HSV-1 titer when 0.25 mg/ml F2 fraction was tested and a reduction of 2.7 log10in VSV virus titer and of 1.5 log10in HSV-1 virus titer was observed when 0.25 mg/ml F3 fraction was tested. Results obtained in this work suggest a potential pharmaceutical use of TG extract components.


Previamente se han descripto distintas actividades inmunomoduladoras, presentes en extractos de hojas de Trichilia glabra (TG). En particular, se ha demostrado una actividad antiinflamatoria presente en extractos metanólicos. En este trabajo se investigó la actividad virucida de dichos extractos sobre virus envueltos y desnudos. Distintos extractos metanólicos han inactivado en forma moderada los virus herpes simplex tipo 1 (HSV-1) y el virus de la estomatitis vesicular (VSV), mientras no evidenciaron actividad sobre poliovirus tipo 3. VSV resultó algo mas afectado que HSV-1: se observó una reducción en el título viral de 2,8 log10para VSV y de 2,4 log10para HSV-1 cuando se uso una concentración de 0,25 mg/ml de la fracción F2 y una reducción de 2,7 log10para VSV y de 1,5 log 10para HSV-1 cuando se usó una concentración de 0,25 mg/ml de la fracción F3. Los resultados obtenidos en este trabajo, sugieren un potencial uso farmacéutico de los componentes presentes en los extractos de TG.


Subject(s)
Animals , Antiviral Agents/isolation & purification , Meliaceae/chemistry , Plant Extracts/pharmacology , Plant Leaves/chemistry , Antiviral Agents/pharmacology , Chlorocebus aethiops , Chemical Fractionation , Chloroform , Herpesvirus 1, Human/drug effects , Methanol , Plant Extracts/isolation & purification , Poliovirus/drug effects , Vero Cells , Vesicular stomatitis Indiana virus/drug effects
11.
Chinese Journal of Biotechnology ; (12): 130-135, 2004.
Article in Chinese | WPRIM | ID: wpr-305214

ABSTRACT

The gene encoding the nucleocapsid (N) protein of vesicular stomatitis virus (VSV-NJ) was subcloned from pMD-VN5, and inserted into pBAD/Thio TOPO vector. The recombinant plasmid was identified by restriction analysis and PCR. It was sequenced to confirm the correct sequences and the correct junctional orientations of the inserted N gene. The results of SDS-PAGE and Western immunoblotting revealed that the N protein was expressed in Escherichia coli LGM194 in a high level and the recombinant fusion protein, which contained a N-terminal HP-Thioredoxin and a C-terminal polyhistidine tag. It had a molecular mass of approximately 63.5 kD and immunologically reactive activity. The recombinant protein was characterized and tested in an enzyme-linked immunosorbent assay (ELISA) format for potential application in the serodiagnosis of vesicular stomatitis using 186 serum samples from experimentally infected goats and guinea-pigs with VSV-NJ and VSV-IN, and from field origin and reference serum samples. The sensitivity and specificity of the ELISA were compared with those of the standard microtiter serum neutralization (MTSN) tests. The ELISA and MTSN test results were highly correlated for detection of VSV antibodies. The ELISA was as sensitive as the SN assay in detecting positive serum to VSV. The correlation between SN titers and ELISA titers was statistically significant. These data suggest that the recombinant fusion N protein of VSV could be used as a recombinant test antigen for the serodiagnosis of vesicular stomatitis. The ELISA based on the reconmbinant nucleocapsid protein may offer the best combination of rapidity, sensitivity, simplicity, economy, and laboratory biosafety of any of the methods yet developed for VSV serodiagnosis. This study lay on foundation for the development of the diagnosis methods in serology for VSV.


Subject(s)
Amino Acid Sequence , Animals , Cloning, Molecular , Enzyme-Linked Immunosorbent Assay , Methods , Molecular Sequence Data , Neutralization Tests , Nucleocapsid Proteins , Chemistry , Genetics , Allergy and Immunology , Recombinant Proteins , Allergy and Immunology , Serologic Tests , Vesicular stomatitis Indiana virus , Genetics , Vesicular stomatitis New Jersey virus , Genetics
12.
Semina cienc. biol. saude ; 24: 11-20, jan.-dez. 2003. ilus, graf
Article in Portuguese | LILACS | ID: lil-412463

ABSTRACT

O vírus da estomatite vesicular (VEV) é um vesiculovírus da família Rhabdoviridae que infecta mamíferos e causa lesões vesiculares semelhantes às observadas na febre aftosa. A encefalite experimental pode ser induzida em roedores e os sintomas são semelhantes aos observados na raiva; entretanto, as lesões observadas no encéfalo dos animais são diferentes. Corpúsculos de inclusão não são observados, há necrose especialmente da região do bulbo olfatório e em alguns casos, ventriculite. Observamos que o padrão temporal de disseminação do VEV e os aspectos morfológicos das lesões são similares aos descritos na literatura. O vírus parece se disseminar através dos ventrículos cerebrais, multiplicando-se em células do epêndima e em neurônios, além de utilizar o transporte retrógrado e anterógrado. Constatamos que devido à facilidade de manipulação do vírus, este modelo experimental tem sido utilizado em inúmeros trabalhos de pesquisa em diversas áreas. Se por um lado, os relatos sobre a patogenia da infecção são numerosos, por outro lado, ainda existem muitas lacunas que envolvem, por exemplo, aspectos sobre a transmissão do vírus, a recuperação dos animais infectados e a participação de células gliais durante a fase aguda e a fase de recuperação dos animais


Subject(s)
Animals , Mice , Disease Models, Animal , Encephalitis, Viral/virology , Vesicular Stomatitis/virology , Vesicular stomatitis Indiana virus/pathogenicity , Acute Disease
13.
Braz. j. vet. res. anim. sci ; 40(1): 29-35, 2003. tab
Article in Portuguese | LILACS, SES-SP | ID: lil-360563

ABSTRACT

No ano de 2000 foram pesquisadas 1099 amostras de soro de bovinos de corte, adultos, pertencentes à seis rebanhos situados nos municípios de Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, região de Araçatuba. A técnica empregada para a dosagem de anticorpos para o vírus da Estomatite Vesicular foi a soroneutralização em microplacas, utilizando a cepa Indiana 1 Costa Rica/72 com título de 10(6,0) DICT50/50mL. As células utilizadas foram as da linhagem de Rim de Macaco Verde Africano (VERO-CCL 81), provenientes do American Type Cell Colection (ATCC). O título de anticorpos de cada amostra de soro, foi calculado como a recíproca da maior diluição do soro expresso em log10, que protegeu 100,00 por cento da monocamada celular. Foram consideradas positivas as amostras com título superior a 1,6 log10 (1/40). Das 1099 amostras de soro analisadas, 28 (2,50 por cento) foram positivas ao vírus da Estomatite Vesicular tipo Indiana 1, com títulos de anticorpos variando de 1,9 à 2,8. Com exceção do rebanho situado no município de Turiúba, nos outros cinco foram encontrados animais reagentes ao vírus. Esta avaliação mostrou que houve circulação do vírus da Estomatite Vesicular nestes rebanhos provocando resposta humoral. Sendo assim é necessário a realização de estudos para se determinar o sítio de permanência do vírus.


Subject(s)
Animals , Adult , Cattle , Antibodies , Vesicular stomatitis Indiana virus
14.
Rev. colomb. cienc. pecu ; 15(1): 100-106, abr. 2002. tab, graf
Article in Spanish | LILACS | ID: lil-474012

ABSTRACT

Este informe da cuenta de los resultados de un proyecto encaminado a determinar la posible diversidad fenotípica de la resistencia/susceptibilidad, in vitro, de la raza de ganado criollo colombiano, Blanco Orejinegro (BON), a la infección por virus de estomatitis vesicular (EV) y de Rinotraqueitis Infecciosa Bovina (RIB). Se probaron 47 muestras de fibroblastos primarios de igual número de animales, mediante titulación viral, y se determinó la Dosis Infecciosa Mínima 50 por ciento por ml (DIM50 por ciento/ml) por el método de Spearman karber. Luego se obtuvieron los Índices de Resistencia/ Susceptibilidad (IRS) y se agruparon los cultivos primarios de fibroblastos en resistentes y susceptibles con los siguientes resultados: Para RIB los 47 cultivos primarios de fibroblastos de ganado BON resultaron susceptibles; para EV serotipo Indiana, 37 fueron susceptibles y 10 resistentes, y para EV serotipo New Jersey, se encontraron 41 susceptibles y 5 resistentes. Un polimorfismo fenotípico en resistencia/susceptibilidad, in vitro, del ganado BON, se había demostrado previamente para el virus de la fiebre aftosa.


Subject(s)
Cattle , Cattle , Cattle Diseases , Immunity, Innate , Infectious Bovine Rhinotracheitis , Infections/veterinary , Pathology, Veterinary , Genetic Techniques/veterinary , Vesicular stomatitis Indiana virus
15.
Rev. méd. Chile ; 129(9): 1079-1084, sept. 2001. tab
Article in Spanish | LILACS | ID: lil-302041

ABSTRACT

This paper analyses the information of the Ministry of Health on gallbladder cancer mortality in Chile since 1997. It becomes evident that the decrease in mortality in the last two years is only apparent and due to a statistical artifact, caused by the non validated application of the Tenth International Classification of Diseases. There is a consensus that one of the causes for an increase in gallbladder cancer in a specific country is a decrease in cholecystectomy rates. This association has been clearly demonstrated in Chile, but no control program for gallbladder cancer has been devised, considering that an early cholecystectomy is a good secondary prevention measure


Subject(s)
Humans , Male , Female , Gallbladder Neoplasms , Chile , Cholecystectomy , Common Bile Duct , Common Bile Duct Diseases/epidemiology , Gallbladder Neoplasms , Hospital Statistics , Vesicular stomatitis Indiana virus
16.
Braz. j. microbiol ; 32(1): 52-5, Jan.-Mar. 2001. tab
Article in English | LILACS | ID: lil-297667

ABSTRACT

Four Lentinula edodes strains (Le10, 46, K2, Assai) were assessed for their antagonistic effect on four filamentous fungus species of agricultural importance (Helminthosporium euphorbiae, Helminthosporium sp, Fusarium solani and Phomopsis sojae) and on Alagoas serotype of Vesicular Stomatitis Virus (VSA). The L. edodes strains studied had variable effects on the filamentous fungi and on VSA. The K2 and Le10 strains were antagonistic on the fungi assessed and the 46 and K2 strains were efficient on the Vesicular Stomatitis Virus. The results widened the list of beneficial effects of L. edodes on the control and prevention of animal pathogenic virus and filamentous fungi.


Subject(s)
Fungi , Lentinula , Vesicular stomatitis Indiana virus
17.
Rev. colomb. cienc. pecu ; 14(1): 20-27, 2001. tab, graf
Article in Spanish | LILACS | ID: lil-474025

ABSTRACT

Se realizó un seguimiento bimensual durante un año, en dos fincas lecheras del Municipio de Fredonia, Antioquia, en mamíferos silvestres, vectores incriminados y en hatos centinelas, con el objetivo de determinar por seroneutralización los porcentajes de animales infectados con el virus de la Estomatitis Vesicular, realizar intentos de aislamiento viral en cada una de estas poblaciones, y detectar fragmentos del genoma viral por RT-PCR y PCR anidado. Se encontró que el porcentaje de infección fue del 57.14 por ciento para el serotipo Indiana (IN) y del 73.01 por ciento para el New Jersey (NJ), igualmente se determinó que el 37.5 por ciento de los animales silvestres capturados mostraron bajos títulos de anticuerpos, 1:8 principalmente y solamente contra el serotipo IN, lo cual parece sugerir que el ciclo de infección en la fauna silvestre no está relacionada con el ciclo en los animales domésticos. De otro lado todas las muestras de sangre de reservorios y macerados de insectos procesadas por las técnicas moleculares mencionadas mostraron resultados negativos. Se discuten los resultados y se proponen nuevos estudios.


Subject(s)
Cattle , Animal Diseases , Colombia/epidemiology , Disease Vectors , Mammals , Simuliidae , Vesicular stomatitis Indiana virus
18.
Säo Paulo; s.n; 2001. [56] p. ilus, tab.
Thesis in Portuguese | LILACS | ID: lil-290571

ABSTRACT

Objetivo: Verificar a presença de anticorpos neutralizantes para o vírus de Estomatite Vesicular, em plantéis bovinos criados em regime extensivo, para a produçäo de carne, situados no município de General Salgado, regiäo de Araçatuba, Estado de Säo Paulo. Materiais e Métodos: Foram pesquisadas 1099 amostras de soro de bovinos adultos, pertencentes à 6 propriedades situadas em 6 municípios da micro-regiäo de General Salgado. A técnica empregada para a dosagem de anticorpos para o vírus da Estomatite Vesicular foi a soroneutralizaçäo em microplacas de 96 orifícios, utilizando a cepa Indiana 1 Costa Rica/72 com título de 10.6,0 DICT50/50uL. As células utilizadas foram as da linhagem de Rim de Macaco Verde Africano (VEROCCL 81), provenientes do American Type Cell Colection (ATCC). O título de anticorpos de cada amostra de soro, foi calculado como a recíproca da maior diluiçäo do soro expresso em log10, que protegeu 100 por cento da monocamada celular. Foram consideradas positivas as amostras com título superior a 1,6 log10 (1/40). Resultados: Das 1099 amostras de soro analisadas, 28 (2,6 por cento) foram positivas ao vírus tipo Indiana 1 da Estomatite Vesicular. Em 5 das 6 propriedades avaliadas, foram encontrados animais reagentes ao vírus; em uma das propriedades, situada no município de Turiúba, näo foram encontrados animais reagentes. Conclusöes: Esta avaliaçäo mostrou que anticorpos para o vírus da Estomatite Vesicular nos bovinos criados na micro-regiäo de General Salgado, mesmo sem sinais da doença. Tal fato indica que pode estar ocorrendo uma estimulaçäo antigênica provocando resposta humoral. Sendo assim é necessário a realizaçäo de estudos para se determinar o sítio de permanência do vírus.


Subject(s)
Animals , Cattle , Antibodies , Cattle Diseases , Rhabdoviridae Infections , Stomatitis , Vesicular stomatitis Indiana virus/immunology
19.
Rev. bras. ciênc. vet ; 6(3): 151-5, set.-dez. 1999. ilus, tab
Article in Portuguese | LILACS | ID: lil-285726

ABSTRACT

Foram realizadas inoculaçöes experimentais do vírus da Estomatite Vesicular Alagoas (VSA) em tilápia nilótica (Oreochromis niloticus), pela via intraperitoneal e por imersäo em suspensäo viral, com o objetivo de verificar se o mesmo se replicava neste sistema hospedeiro e se o vírus era eliminado na água, o que poderia representar um importante papel no ciclo epidemiológico da virose. Os peixes foram dispostos em grupos testemunhos e inoculados com suspensäo de vírus VSA, tanto pela via intraperitoneal quanto por imersäo. Foram observadas alteraçöes anatomopatológicas em órgäos da cavidade abdominal e cérebro. Esses órgäos e a água do aquário foram inoculados em células BHK21 e observados a presença de efeito citopático característico dos vírus pertencentes a este gênero. A segunda passagem do vírus em peixes confirmou as lesöes encontradas anteriormente e o isolamento em novas inoculaçöes em cultivo celular. Os resultados sugerem que a tilápia é sensível ao VSA nas duas vias de inoculaçäo, elimina formas infectantes do vírus na água, podendo representar um importante elo na cadeia epidemiológica da estomatite vesicular.


Subject(s)
Animals , Fish Diseases/epidemiology , Rhabdoviridae Infections/epidemiology , Rhabdoviridae Infections/veterinary , Stomatitis/epidemiology , Stomatitis/veterinary , Tilapia/virology , Vesicular stomatitis Indiana virus , Fishes/virology
20.
Alexandria Journal of Pharmaceutical Sciences. 1998; 12 (2): 95-99
in English | IMEMR | ID: emr-47461

ABSTRACT

Cytotoxicity and antiviral activities of 38 flavones and flavonols were determined. Several general trends were observed, but the lack of consistent structure-activity relationship suggested the need to conduct additional studies under conditions which measure antiviral activity by a single mechanism or at a discrete point in the virus replication cycle. Consistent with this, seven flavonoids that exhibited substantial cytotoxicity and /or antiviral activity were examined for direct viricidal action on isolated virions prior to infection


Subject(s)
DNA Viruses , RNA Viruses , Herpesvirus 1, Human , Vesicular stomatitis Indiana virus
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