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Objective To investigate the effect of ropivacaine on biological behavior of gastric cancer BGC-823 cells and AMP activated protein kinase(AMPK)/nuclear factor E2-related factor 2(Nrf2)signaling path-way.Methods Gastric cancer BGC-823 cells were cultured in vitro.When BGC-823 cells were cultured to log-arithmic growth phase,they were divided into control group(routine culture),cisplatin group(medium con-taining 5 mg/L cisplatin),and low-level(medium containing 25 mg/L ropivacaine),medium-level(medium containing 50 mg/L ropivacaine),and high-level(medium containing 100 mg/L ropivacaine)ropivacaine groups.The cells were cultured for 48 h.Cell counting kit-8(CCK-8)was used to detect the survival rate of BGC-823 cells.Transwell chamber assay was used to detect the invasion ability of BGC-823 cells.The apopto-sis rate of BGC-823 cells was detected by flow cytometry.The mRNA levels of AMPK and Nrf2 in BGC-823 cells were detected by real-time fluorescence quantitative PCR(qPCR).The protein levels of AMPK and Nrf2 in BGC-823 cells were detected by Western blot.Results Compared with the control group,the survival rate,invasion number,AMPK and Nrf2 mRNA and protein levels of BGC-823 cells in the cisplatin group and the low-level,medium-level and high-level ropivacaine groups were decreased(P<0.05),while the apoptosis rate was increased(P<0.05).Compared with the cisplatin group,the survival rate,invasion number,AMPK and Nrf2 mRNA and protein levels of BGC-823 cells in the low-level,medium-level and high-level ropivacaine groups were increased(P<0.05),while the apoptosis rate was decreased(P<0.05).Compared with the low-level ropivacaine group,the survival rate,invasion number,AMPK,Nrf2 mRNA and protein levels of BGC-823 cells in the medium-level and high-level ropivacaine groups were decreased in oder(P<0.05),while the apop-tosis rate was increased in oder(P<0.05).Conclusion Ropivacaine could inhibit the survival and invasion of BGC-823 cells and promote the apoptosis of BGC-823 cells,which may be related to the inhibition of AMPK/Nrf2 signaling pathway.
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Objective To explore the feasibility of laparoscopic surgery in the treatment of pediatric periappendicural abscess.Methods A total of 22 cases of pediatric periappendicural abscess were treated with laparoscopic surgery from April 2013 to May 2016.In the operation, we performed the separation from the side of the abdominal wall and abscess adhesion, and then lifted the abscess for blunt separation into the abscess cavity.After the management of appendix stump, the abdominal cavity was washed and a peritoneal drainage tube was placed.Results Laparoscopic appendectomy was performed successfully in all the cases.No conversion to open laparotomy was needed.The operation time was (65.3±8.2) min, the intraoperative blood loss was (10.5±4.2) ml, and the postoperative hospitalization was (6.5±1.4) days.Follow-ups for 6-24 months (mean, 12 months) in the 22 cases found no complications, such as abdominal abscess, stump fistula, stump appendicitis or adhesive ileus.Conclusion The application of laparoscopic surgery in the treatment of pediatric periappendicural abscess is feasible and safe.
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Objective To evaluate the clinical effect of single-site umbilical laparoscopy in the treatment for inguinal incarcerated hernia in children. Methods Retrospective reviews were conducted for the clinical data of the 105 children with inguinal incarcerated hernia during January 2010 to August 2014. According to different surgical approaches, data were divided into single-site laparoscopic surgery group (SLS, n=56) and open surgery group (OS,n=49). The follow-up period was 6-36 months. Results Two groups of patients were successfully completed surgery. The average operative duration was significantly longer in OS group (33.73±4.41)min than that of SLS group (26.14±5.17) min (P<0.01). The average operative blood loss was (3.18 ± 2.90) mL for OS group versus (1.73 ± 0.88) mL for SLS group (P<0.01). The hospitalization duration was (4.22±1.16) d for OS group versus (3.50±0.97) d for SLS group (P<0.01). The postoperative complications were 24.5%(12/49) for OS group and 8.9%(5/56) for SLS group. There was no recurrence of hernia in two groups. Conclusion Single-site umbilical laparoscopy is safe and efficacious for treatment of inguinal incarcerated hernia in children, which offers a new choice of treatment for occult inguinal hernia.
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OBJECTIVE; To review the molecule mechanism of multidrug resistant osteosarcoma cell apoptosis induced by laser. DATA SOURCES; An online search of Pubmed database and CNKI database was undertaken to identify the articles published from 1996 to 2006 by using the keywords of “laser, osteosarcoma and apoptosis”, and language was limited to both Chinese and English. STUDY SELECTION; The articles were primarily screened and then the full-texts were searched. Included criteria: relevant to the mechanism of tumor cell apoptosis induced by laser. Articles were excluded because of repetition and obsolescence. DATA EXTRACTION; Totally 74 articles were found and 31 of them were selected according to the inclusive criteria as described above. Of the 31 articles, 5 articles were related to reactive oxygen species, 5 were associated with intracellular calcium concentration, 6 were involved in protein kinase C and 5 in Bcl-2 respectively. The last 8 were about P53 in the tumor apoptosis induced by laser. DATA SYNTHESIS; The mechanisms that laser induces osteosarcoma cell apoptosis include mainly reactive oxygen species, intracellular calcium concentration, protein kinase C, Bcl-2, p 53 and so on. The recent reports focus on the proliferation improvement of low-intensity laser on normal cells, but few reviews the biologic effect of tumor cells, especially multidrug resistant osteosarcoma and animal models. It is presumed to be correlated with the mechanism involved above. CONCLUSION; The mechanism that laser induces osteosarcoma cell apoptosis is still unclear, and is mainly related to reactive oxygen species, intracellular calcium concentration, protein kinase C, bcl-2, p 53 and so on.
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0.05).Conclusion Semiconductor laser can induce apoptosis of MG-63 cells by mitochondrion pathway,and the process may be associated with the increasing of ROS.
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Objective To summarize our experience on laparoscopic appendectomy combined with high ligation of the vaginal process in children.Methods From January 2004 to May 2007,totally 326 patients with appendicitis underwent laparoscopic appendectomy in our hospital.During the operation,by inguinal exploration,83 cases of vaginal process deformity (17 bilateral and 69 unilateral lesions) were confirmed.High ligation of the vaginal process was performed on these patients.Results The operation was successfully done in all of the 83 cases,none of them showed postoperative complications.The cases were followed up for 6 to 12 months,during which only one patient had recurrent inguinal hernia.Three patients developed latent hernia but receined no treatment. Conclusions Laparoscopic appendectomy combined with high ligation of the vaginal process is an safe,effective,and low-cost procedure,leading to quick recovery and less pain for children with appendicitis complicated with vaginal process deformity.
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Objective To evaluate the value of laparoscopic surgery application in treating intussusception.Methods Between January 2004 and December 2007,12 children with refractory (8 cases,failed air enema) or recurrent (for over 3 times) intussusception received laparoscopic surgery in our hospital.Three trocars were used during the surgery.Under a laparoscope,by interrupted suture,the terminal ileum was joined to the ascending colon (3 stitches),and the ileocecal junction was fixed to the right posterior abdominal wall (2 to 3 stitches,only for children that failed air enema).Results The intraoperative blood loss ranged from 20 to 60 ml,and the operation lasted for 45 to 90 minutes.No patient developed anastomotic leakage or abnormal gastrointestinal function.The patients were followed up for 1 to 5 years (mean,2 years and 9 months),during which none of them had recurrence. The children had normal intake and excretion,and developed well.Conclusions Children with intussusception recover quickly after laparoscopic treatment with a few postoperative complications.