The mTOR pathway inhibitor Everolimus in cell autophagy of different gliomas / 中华神经医学杂志

Objective To investigate the mTOR pathway inhibitor Everolimus in cell autophagy of different human gliomas in vitro. Methods Glioma cell lines,87-MG,U251 and SHG- 44,were cultured in vitro; different doses of Everolimus (0.1,0.5,1,10,20 and 100 nmol/L) were given to these cells for 4-6 d; MTT assay was used to observe the proliferation of these human glioma cells and the IC50 value was observed; Hoechst 33342/PI staining was employed to determine the apoptosis after Everolimus treatment at dosage of IC50 value for 48 h; monodansyl cadaverine (MDC) staining was used to detect the autophagic vesicles after Everolimus treatment at dosage of IC50 value for 48 h; flow cytometry was employed to detect the effect of Everolimus at IC50 value for 48 h on quantitative cell cycle; Western blotting was employed to further analyze the protein expressions ofphosphorylated and non-phosphorylated mTOR,p70S6K and 4E-BP-1 ofglioma cells after Everolimus treatment at IC50 value for 24 h. Results The U87-MG,SHG-44 and U251 cell growth was significantly inhibited,and their IC50 value was 0.1 nmol/L,0.5 nmol/L and 10 nmol/L,respectively; no obvious apoptosis was noted in the cells,which indicated that cell autophagy might exist; the fluorescent particles were gradually increased as the increment of Everolimus dosages; Everolimus could cause the cycle arrest at the G0/G1 phase in these 3 kinds of cells; the expressions of phosphorylated mTOR,p70S6K and 4E-BP-1 were significantly decreased, and the expression of non-phosphorylated 4E-BP-1 was decreased, while that of non-phosphorylated p70S6K showed no significant changes. Conclusion The mTOR pathway inhibitor Everolimus can induce the cell autophagy ofgliomas,and mTOR pathway might be a potential new therapeutic target in treating glioma.

Value of ECG monitoring in prognosis prediction of aneurysmal subarachnoid hemorrhage / 中华神经医学杂志

Objective To investigate the variation of electrocardiogram (ECG) in the course of aneurysmal subarachnoid hemorrhage (SAH) for predicting the prognosis. Methods The in-hospital data of 108 cases with aneurysmal SAH who had underwent ECG re-examination were retrospectivelyanalyzed to find out the relationship between characters of ECG and prognosis. Results Of the 108 cases, aneurysm clamping by eraniotomy was performed in 41 and endovascular intervention in 67; a total of 89 cases (82.4%) presented abnormal ECG primitively, 62 patients had 2 kinds of ECG changes at least; 71 patients presented abnormal ST-T, 13 cases with abnormal cardiac rhythm, 14 cases with delayed Q-T intermediate stage, 37 cases with abnormal T wave, 9 cases with abnormal Q wave, and 7 cases with other changes; 99 cases recovered in ECG re-examination, 5 cases partly recovered and 4 cases aggravated. GOS score was 4-5 in 98 cases, 3 in 7 cases, and 1-2 in 3 cases. Conclusion Though the type of ECG variation has no significant correlation with SAIl prognosis, ECG monitoring during the course of SAH is of important value for predicting the prognosis.

Effect of hyperthermia combined with trauma on serum nitric oxide and mean arterial pressure in rabbits / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To study the early change of serum nitric oxide (NO) after acute heat exposure with trauma and the effect of NO on mean arterial pressure (MAP), thus to provide theoretical basis for studying the mechanism of NO effect in acute stress.</p><p><b>METHODS</b>The rabbit model of acute heat exposure combined with trauma was established. The animals were divided into four groups, including control, trauma, hyperthermia and hyperthermia combined with trauma. The levels of NO were measured at different time points: 0 h, 1 h, 2 h and MAP was monitored throughout the whole experiment.</p><p><b>RESULTS</b>The concentration of NO declined at first and then increased at 1 h or so after acute heat exposure and trauma. The levels of NO in hyperthermia with trauma group at 1 h, 2 h were (42.75 +/- 8.24), (59.54 +/- 9.05) micro mol/L respectively (P < 0.05), while those in control group were (56.63 +/- 3.79) and (55.22 +/- 7.15) micro mol/L, the difference at 1h between two groups was significant (P < 0.05). Under the circumstance of hyperthermia and trauma, the level of MAP declined to the lowest point at 60 - 70 min and then showed a transient rise, after that, the level declined rapidly.</p><p><b>CONCLUSIONS</b>At the early stage of acute heat exposure and trauma, the concentration of serum NO declined at first and then increased, and had certain relationship with the change of MAP.</p>