Biocompatibility of combined deproteinized bone coated with hepatocyte growth factor as scaffold for osteoblasts in vitro in fetal rabbits / 中南大学学报(医学版)

OBJECTIVE@#To determine the cellular compatibility of combined deproteinized bone(DPB) coated with hepatocyte growth factor (HGF), and to observe the adherent effect of osteoblasts in response to HGF.@*METHODS@#Osteoblasts were isolated from fetal rabbits. Osteoblasts were cultured with DPB coated with HGF and deproteinized bone as experimental group and contral group, respectively. The proliferation and alkalinephosphatase activity were tested. Their growth was examined by inverted phase contrast microscope and scanning electronmicroscope.@*RESULTS@#The osteoblasts were attached to the outside and inside surfaces and grew well. HGF/DPB could stimulate the alkalinephosphatase activity of the osteoblasts and improve the proliferation of the osteoblasts.@*CONCLUSION@#HGF/DPB has good biocompatibility and bone induction. HGF could improve the adherent effect of DPB on osteoblasts, and it could be used as scaffold material for the bone tissue engineering.

The transplantation of latissimus dorsi flap of the base of T shape artery with the pedicle of the thoracodorsal artery / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore the possibility that the free latissimus dorsi musculo-cutaneous flap to repair the forearm leg wound.</p><p><b>METHODS</b>To design latissimus dorsi musculo-cutaneous flap which is foundation on T form thoracodorsal artery stalk. To set the short arm into the receiver artery break and anastomos them. It is not only reassure the blood of free musculo-cutaneous flap, but also reconstruct the continuation of the receiver main artery.</p><p><b>RESULTS</b>In 16 patients, 15 patients success completely, 1 patient main success. The blood supply of receiver is adequate.</p><p><b>CONCLUSIONS</b>The free T form thoracodorsal artery stalk musculo-cutaneous flap free grafting is a good method to repair the skin and soft tissues defection of forearm and leg.</p>