Preparation of Angiotico-2 (A-2) an Electrohomeopathic Remedy, its TLC, FTIR, Pharmacognocalogical, Phytochemical, Chemical, Heavy Metal, Pesticide, Food Additives and Microbial Screening for Safe Usage

To evaluate phytochemical, heavy metal, pesticide, microbial assay, and also FTIR studies of Angiatico-2 (A-2) an Electrohomeopathic remedy to evaluate safety and efficacy for its usage. Electrohomeopathy / Electropathy is one purely herbal medical system invented by Italian C. C. Mattie (1809-1896) has been practiced since the 1860s across the world. Electrohomeopathy has its own unique principles, plants selection, the process of remedies preparation, diagnosis, selection, and combination of drugs for different diseases, dosage, and treatment methods. In India, it is estimated that there are about 450-500 institutions imparting education and research, about 4.5 to 5 lacks practitioners are practicing and millions of population getting benefits currently. The government of India initiated steps to recognize Electrohomeopathy medical system under the constitution. Unfortunately, there is very limited scientific evidence to evaluate the safety, efficacy, phytochemical, and pharmacology studies in Electrohomeopathy. We prepared Angiatico-2 an Electrohomeopathic remedy as Mother Solution and D4 dilutions and evaluated it’s phytochemical screening, TLC, FTIR spectrum, Physical and Chemical nature, presence or absence of heavy metals, Pesticide, Food Additives and Microbial under NABL standards for its safety usage. Electrohomeopathic herbal remedy Angiatico-2 prepared by using 7 non poisonous plants by Krauss method under standard condition and not shown any heavy metals, pesticide presence and also no microbial presence above the limit level of FSSAI standard. Present studies of Electrohomeopathic herbal remedy Angiatico-2 preparation and its screening in standard scientific laboratory condition, TLC & FTIR studies may become reference standards for the manufacture of Angiatico-2 for bulk quantity at industrial level for. The present research investigations outcome with reproducibility may become standard markers or signatures to assess the quality and safe use of Angiatico-2.

Comparative evaluation of low-level laser therapy and ultrasound heat therapy in reducing temporomandibular joint disorder pain

BACKGROUND: Pain, limitations in opening, asymmetrical jaw movements, and temporomandibular joint (TMJ) sounds are the most common findings in temporomandibular joint disorders (TMDs), which causes excruciating pain, inflammation of the surrounding muscles, posterior fibers, and synovial fluid. This study aimed to evaluate and compare the effects of ultrasound heat therapy and low-level laser therapy (LLLT) in reducing TMD-related pain. METHODS: This prospective study included 42 patients (age range, 25–45 years), who were divided into two groups of 21 patients each. All patients were prescribed a non-steroidal anti-inflammatory drug (NSAID) twice a day for 5 days for temporary relief of pain prior to the commencement of treatment. Patients were kept on a soft diet and asked to restrict mouth opening during the same period. Fifteen sessions of LLLT (Group A) or ultrasound therapy (Group B) were administered to the affected side. RESULTS: Post-therapy, the mean visual analog scale score for group A and group B was 4.81 (2.01) and 6.19 (1.20), respectively; the difference was statistically significant and favoring the LLLT group. Similarly, the mean mouth opening for group A and group B was 3.99 (0.40) and 3.65 (0.41), respectively; the difference was statistically significant and favoring the LLLT group. CONCLUSION: Our study recommends LLLT for treating TMD-related pain with no underlying bony pathology.

Evaluation of cognitive function of fluoxetine, sertraline and tianeptine in isolation and chronic unpredictable mild stress-induced depressive Wistar rats.

Depressive illness is generally associated with cognitive impairment. Serotonergic selective antidepressant drugs, fluoxetine (FLX), sertraline (SER) and tianeptine (TIA), are claimed to have less or no effect on cholinergic system, the key system involved in memory. In the present study, these drugs were evaluated for their influence on cognitive behavior in both depressive and non-depressive animals. Depression was induced by two models, (i) 60 days social isolation of litter; and ii) by applying chronic unpredictable mild stress for 21 days. Depression in the rats was confirmed by behavioral despair test. Transfer latency on elevated plus maze and inflexion ratio in passive avoidance step through behavior were employed to assess learning and memory. The results indicated that administration of fluoxetine; sertraline and tianeptine attenuated the cognitive deficits observed in depressive rats. In non-depressive rats these drugs produced retention deficit, which was found to be parameter and model dependent. Data suggested that, FLX and SER (SSRI's) effectively attenuated the isolation-induced depression and cognitive deficit, whereas TIA (SSRE) produced better effect in stress-induced depressive conditions. It was concluded that behavioral profiles of fluoxetine, sertraline and tianeptine on cognition were model and parameter dependent.

Artificial neural network and medicine.

The introduction of human brain functions such as perception and cognition into the computer has been made possible by the use of Artificial Neural Network (ANN). ANN are computer models inspired by the structure and behavior of neurons. Like the brain, ANN can recognize patterns, manage data and most significantly, learn. This learning ability, not seen in other computer models simulating human intelligence, constantly improves its functional accuracy as it keeps on performing. Experience is as important for an ANN as it is for man. It is being increasingly used to supplement and even (may be) replace experts, in medicine. However, there is still scope for improvement in some areas. Its ability to classify and interpret various forms of medical data comes as a helping hand to clinical decision making in both diagnosis and treatment. Treatment planning in medicine, radiotherapy, rehabilitation, etc. is being done using ANN. Morbidity and mortality prediction by ANN in different medical situations can be very helpful for hospital management. ANN has a promising future in fundamental research, medical education and surgical robotics.

Enhanced photosynthesis in cytozyme-treated blackgram, Phaseolus mungo L.

Foliar application of cytozyme to 30-day-old black-gram plants resulted 48% increase of dry matter accumulation. The increase in fresh and dry weights of total plants was largely due to enhanced CO2 assimilation rates which were associated with increased RuBP carboxylase activities. The photochemical characteristics in the isolated chloroplasts exhibited an increase of 32, 28 and 40%, measured as the photoreduction of DCPIP, FeCN and NADP, respectively. Cytozyme treatment also resulted an increase in the chlorophyll content in leaves.

Dermal toxicity of hexachlorocyclohexane (HCH) in rabbit.

Application of HCH (25 mg/kg) on dorsal, ventral and thigh regions of the skin of male rabbits resulted in poisoning and mortality of animals. Morphological changes in skin, liver, kidney, testes and cerebellum together with highly significant alterations in serum and liver enzymatic activity and residue in blood suggested that absorption of HCH and its toxicity could be severe when the pesticide comes in contact with the skin of thigh region of body.