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ABSTRACT Objective: to explore associations and gender differences between OHI-seeking (online health information seeking) behaviors and eHealth (electronic health) literacy among Chinese university students. Methods: Online questionnaires of eHealth literacy scale and OHI-seeking behaviors created in software Wenjunxing were used in this survey. Chi-squared tests, t-test, and Pearson correlation analysis were performed using SPSS for data analysis. Results: Among 5,383 participants, 72.4% were girls, 77.5% lived in rural areas, 51.2% majored in liberal arts, 76.6% with low education parents. The average C-eHEALS scores of boys and girls were 26.53 ± 5.861 and 26.84 ± 5.816, respectively, with no significant difference (P = 0.084). The top three OHI-seeking behaviors for boys and girls, as well as for the C-eHEALS low and high groups, were "finding information about physical exercises" "reading or sharing health information via social media" "finding information about nutrition and diet", all of which had significant gender and eHealth literacy differences. Conclusions: Gender and eHealth literacy differences should be focused for intervention when developing and implementing eHealth intervention training for parents and adolescents in schools and communities.
RESUMO Objetivo: Visa explorar associações e diferenças de gênero entre comportamentos de busca de OHI (busca de informações de saúde on-line) e letramento em e-Saúde (saúde eletrônica) entre estudantes universitários chineses. Métodos: Questionários on-line da escala de letramento em e-Saúde e comportamentos de busca de OHI criados no software Wenjunxing foram utilizados nesta pesquisa. Testes qui-quadrado, teste t e análise de correlação de Pearson foram realizados utilizando SPSS para análise de dados. Resultados: Dos 5.383 participantes, 72,4% eram meninas, 77,5% viviam em áreas rurais, 51,2% eram formados em artes liberais, 76,6% tinham pais com baixa escolaridade. As pontuações médias do C-eHEALS de meninos e meninas foram 26,53 ± 5,861 e 26,84 ± 5,816, respectivamente, sem diferença significativa (P = 0,084). Os três principais comportamentos de busca de IHO para meninos e meninas, bem como para os grupos baixo e alto do C-eHEALS, foram "encontrar informações sobre exercícios físicos"; "ler ou compartilhar informações de saúde através das redes sociais"; "encontrar informações sobre nutrição e dieta", todos com diferenças significativas de gênero e de letramento em e-Saúde. Conclusões: As diferenças de gênero e de letramento em e-Saúde devem ser focadas na intervenção ao desenvolver e implementar formação de intervenção em e-Saúde para pais e adolescentes em escolas e comunidades.
RESUMEN Objetivo: Explorar las asociaciones y las diferencias de género entre el comportamiento de búsqueda de OHI (información sanitaria en línea) y los conocimientos sobre e-Salud (salud electrónica) entre estudiantes universitarios chinos. Métodos: Se utilizaron cuestionarios en línea de la escala de conocimientos en e-Salud y conductas de búsqueda de OHI creadas con el software Wenjunxing. En el análisis de los datos, se utilizó el programa SPSS para llevar a cabo las pruebas de chi-cuadrado, pruebas t y análisis de correlación de Pearson. Resultados: De los 5.383 participantes, el 72,4% estaba formado por muchachas, el 77,5% vivía en zonas rurales, el 51,2% era licenciado en Humanidades y el 76,6% tenía padres con bajo nivel educativo. Las puntuaciones medias del C-eHEALS para chicos y chicas fueron 26,53 ± 5,861 y 26,84 ± 5,816, respectivamente, sin diferencias significativas (P = 0,084). Las tres conductas principales de búsqueda de la OHI para muchachos y muchachas, así como para los grupos de C-eHEALS bajo y alto, fueron "encontrar información sobre ejercicio físico"; "leer o compartir información sobre salud a través de las redes sociales"; "encontrar información sobre nutrición y dietas", todos ellos con diferencias significativas de género y de aprendizaje sobre e-Salud. Conclusiones: Las diferencias de género y de conocimientos en e-Salud deben centrarse en la intervención a la hora de desarrollar e implementar la formación en cibersalud para padres y adolescentes en escuelas y comunidades.
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Humanos , Educación en Salud , Alfabetización en Salud , Identidad de Género , Estudiantes , Gestión de la Información en SaludRESUMEN
@#Abstract: Objective To analyze the application effect of rapid diagnostic techniques in Shaanxi from 2016 to 2020,and to provide basis for further optimizing the process of tuberculosis detection and formulating prevention and control strategies. Methods A total of 104 437 cases of tuberculosis patients registered in Shaanxi Province from 2016-2020 were exported from the Tuberculosis Information Management System (The subsystem of China Disease Prevention and Control Information System) according to first management unit, and the laboratory test results of sputum smear, sputum culture and molecular tests were collected to statistically analyzed the positive rate of etiology, sputum smear, sputum culture, molecular biology testing rate, and indicators of positive testing rate of tuberculosis patients. Results From 2016 to 2020, the etiology�positive rate of tuberculosis in Shaanxi province were 13.49% (2 664/19 754), 22.68% (5 081/22 401), 35.99% (8 232/22 876), 48.14% (10 438 / 21 682), 52.65% (9 332 / 17 724), respectively, with an increasing trend (χ2 trend=9 473.12, P<0.001) year by year; the proportion of molecular tests positive only in etiology-positive pulmonary tuberculosis (PTB) were 0 (0/2 664), 0.16% (8/5 081), 15.44% (1 271/8 232), 27.58% (2 879/10 438), 31.52% (2 941/9 332), respectively, with an increasing trend year by year (χ2 trend=2 971.44, P<0.001); the molecular test rates of the 5 years were 0.01% (2 / 19 754), 0.38%(85 / 22 401), 21.11% (4 828/22 876), 52.42%(11 365/21 682), 55.18%(9 780/17 724), respectively, with an increasing trend year by year (χ2 trend = 28 269.23, P<0.001). The rate of molecular test in sputum smear-negative was 22.72%(17 976 / 79 130). The proportion of patients with only molecular test-positive was 33.43% (4 032/12 062) in municipal designated hospitals, and 11.99%(2 279/ 19 014) in county-level designated hospitals, the difference was statistically significant (χ2 =2 096.46, P<0.001). Conclusions The rate of molecular biology testing in Shaanxi Province from 2016 to 2020 showed a year-on-year increase. Through the application of rapid molecular tests, the etiology-positive rates of tuberculosis have been increased significantly,but the current molecular test detection rate is not high compared with other provinces, especially in county-level designated hospitals and smear-negative patients, so we should make a big promotion in application of rapid molecular technique.
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@#Abstract: Objective To investigate the early diagnostic value of peripheral blood procalcitonin (PCT), C-reactive protein (CRP), fibrinogen (FIB) and D-dimer (D-D) levels in patients with pulmonary tuberculosis (PTB) complicated with bacterial pneumonia. Methods A total of 102 patients who admitted to Department of Tuberculosis of Affiliated Nantong Hospital of Shanghai University from Jan 2021 to May 2022 were enrolled in this study and divided into a group (52 cases) with pulmonary tuberculosis (PTB) patients and a group (50 cases) with PTB patients complicated with bacterial pneumonia. The levels of PCT, CRP, FIB and D-D in the peripheral blood were measured, the differences and correlations in all indicators were compared among two groups. The sensitivity and specificity of these indicators in the early diagnosis of PTB complicated with bacterial pneumonia were analyzed by receiver operating characteristic (ROC) curve. Results The levels of PCT, CRP, FIB and D-D in the peripheral blood from the PTB complicated with bacterial pneumonia group were 0.06 (0.04, 0.16) ng/mL, 38.00 (3.88, 96.10) mg/L, 4.51 (3.02, 6.07) g/L, and 0.59 (0.34, 1.88) mg/L, respectively, which were significantly higher than corresponding 0.04 (0.03, 0.04) ng/mL, 3.20 (0.84, 7.22) mg/L, 2.96 (2.48, 3.77) g/L, and 0.27 (0.17, 0.36) mg/L in the PTB group (Z=-4.784, -5.233, -3.853, -4.199, all P<0.001). Furthermore, the levels of CRP and FIB in the PTB complicated by bacterial pneumonia group were highly positively correlated (r=0.855, P<0.001). The area under the ROC curve (AUC) of PCT, CRP, FIB and D-D for early diagnosis of PTB complicated with bacterial pneumonia were 0.757, 0.794, 0.747 and 0.764, respectively. In addition, the AUC obtained by simultaneous measurement of PCT, CRP, FIB and D-D was as high as 0.916, and the sensitivity and specificity of diagnosing PTB complicated with bacterial pneumonia were increased to 85.7% and 96.9%, respectively, which were higher than those of individual indicators. Conclusions Levels of peripheral blood PCT, CRP, FIB, and D-D all show varying degrees of increase in patients with PTB complicated with bacterial pneumonia, and detecting the levels of all four markers, rather than any single marker, can assist in early monitoring whether the tuberculosis patients are complicated with bacterial pneumonia.
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OBJECTIVE@#To investigate the safety and efficacy of novel CD19-KIRS2/Dap12-BB chimeric antigen receptor T cells (CAR-T cells) in the treatment of relapsed/refractory B-cell malignancy (R/R BCM).@*METHODS@#Three patients with R/R BCM treated with novel CD19-KIRS2/Dap12-BB CAR-T cells from June 2020 to November 2020 were enrolled, including 1 case of B-cell acute lymphoblastic leukaemia (B-ALL) and 2 cases of non-Hodgkin's lymphoma (NHL), and the efficacy and adverse reactions were observed.@*RESULTS@#After CAR-T cells infusion, patient with B-ALL achieved complete remission (CR) and minimal residual disease (MRD) turned negative, and 2 patients with NHL achieved partial remission (PR). Grade 2 cytokine release syndrome (CRS) occurred in B-ALL patient, grade 1 CRS occurred in 2 NHL patients, and grade II to IV hematologic adverse reactions occurred in 3 patients, all of which were controllable and reversible. The progression-free survival (PFS) of the 3 patients was 143, 199, and 91 days, and overall survival (OS) was 282, 430, and 338 days, respectively.@*CONCLUSION@#The novel CD19-KIRS2/Dap12-BB CAR-T cells in treatment of 3 patients with R/R BCM have significant short-term efficacy and controllable adverse reactions, but the long-term efficacy needs to be further improved.
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Humanos , Receptores Quiméricos de Antígenos , Inmunoterapia Adoptiva , Linfoma de Burkitt , Antígenos CD19 , Neoplasia Residual , Proteínas Adaptadoras Transductoras de SeñalesRESUMEN
ABSTRACT Introduction: High-intensity interval training (HIIT) refers to multiple short-term high-intensity exercises. Objective: To study the impact of sprint interval training (SIT) on the athletic ability of elite rugby players. Method: 36 male rugby players from a sports college were selected. They were randomly divided into upper limb training (Group A), lower limb training (Group B), and routine training (Group C - control). Group C received routine training for six weeks. Groups A and B added SIT training based on Group C's upper and lower limb training. Results: After training, the maximum aerobic power (MAP) in Groups A and C was significantly higher (P < 0.05), as well as the myeloperoxidase (MPO) of lower limbs in group B (P < 0.05). After completing 5 and 6 sets of SIT training, blood lactic acid (BLA) was lower in group B (P <0.05). Parameters such as upper limb intermittent sprint exercise capacity and lower-limb aerobic exercise capacity did not significantly change compared with the same parameters before training (P>0.05). Conclusions: Excellent male rugby players can improve their upper limb aerobic and lower limb intermittent sprint athletic ability by integrating different methods of SIT training into their daily training. Level of evidence II; Therapeutic studies - investigation of treatment results.
RESUMO Introdução: Treino intermitente de alta intensidade (HIIT) se refere a múltiplos exercícios de alta intensidade num período curto de tempo. Objetivo: Estudar o impacto do treino de sprint intervalado (SIT) na capacidade atlética de jogadores de rúgbi de elite. Método: 36 jogadores de rúgbi do sexo masculino, de uma faculdade esportiva, foram selecionados. Eles foram divididos aleatoriamente entre treino de membros superiores (Grupo A), treino de membros inferiores (Grupo B), e treino de rotina (Grupo C - controle). O Grupo C recebeu treino de rotina por seis semanas. Os Grupos A e B acrescentaram ao treinamento de rotina um treino de SIT feito de acordo com o treino de membros inferiores e superiores realizado pelo grupo controle. Resultados: Depois do treinamento, o poder aeróbico máximo (MAP) dos Grupos A e C se mostrou significativamente mais elevado (p>0,05), enquanto a mieloperoxidase (MPO) dos membros inferiores do Grupo B foi mais alta (p<0,05). Parâmetros como capacidade de exercícios de sprint intermitente dos membros superiores e capacidade de exercícios aeróbicos dos membros inferiores não mudaram significativamente quando comparados antes e depois do treinamento (p>0,05). Conclusões: Jogadores de rúgbi de alto nível podem melhorar sua capacidade atlética de sprint intermitente nos membros inferiores e de exercícios aeróbicos nos membros superiores ao integrar diferentes métodos de treinamento SIT em seu treino diário. Nível de evidência II; Estudos terapêuticos - investigação do resultado de tratamentos.
RESUMEN Introducción: El entrenamiento de intervalos de alta intensidad (HIIT) se refiere a múltiples ejercicios de alta intensidad en un periodo corto de tiempo. Objetivo: Estudiar el impacto del entrenamiento de sprint en intervalos (SIT) en la capacidad atlética de jugadores de rugby de elite. Método: Fueron seleccionados 36 jugadores del sexo masculino de una facultad deportiva. Estos fueron divididos aleatoriamente entre entrenamiento de miembros superiores (Grupo A), entrenamiento de miembros inferiores (Grupo B) y entrenamiento de rutina (Grupo C - control). El Grupo C recibió entrenamiento de rutina por seis semanas. Los Grupos A y B agregaron al entrenamiento de rutina un entrenamiento de SIT hecho de acuerdo con el entrenamiento de miembros inferiores y superiores realizado por el grupo control. Resultados: Después del entrenamiento, el poder aeróbico máximo (MAP) de los Grupos A y C se mostró significativamente más elevado (p>0,05), mientras que la mieloperoxidasa (MPO) de los miembros inferiores del Grupo B fue más alta (p<0,05). Parámetros como capacidad de ejercicios de sprint de intervalos de los miembros superiores y capacidad de ejercicios aeróbicos de los miembros inferiores no cambiaron significativamente cuando fueron comparados antes y después del entrenamiento (p>0,05). Conclusiones: Jugadores de rugby de alto nivel pueden mejorar su capacidad atlética de sprint de intervalos en los miembros inferiores y de ejercicios aeróbicos en los miembros superiores al integrar diferentes métodos de entrenamiento SIT en su ejercitamiento diario. Nivel de evidencia II; Estudios terapéuticos - investigación del resultado de tratamientos.
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ABSTRACT Introduction: The main purpose of aerobic exercise is to enhance cardiopulmonary endurance, so it is necessary to build cardiopulmonary endurance response models based on different frequencies of aerobic exercise. Objective: To study the cardiopulmonary endurance response of women to different frequencies of aerobic exercise. Methods: Twenty young female desk workers (female teachers and civil servants) who worked out at a fitness club were randomly divided into two groups. Cardiopulmonary function, both before and after 16 weeks of aerobic exercise at different exercise loads, was studied and analyzed. Results: After 16 weeks of aerobic exercise at different exercise loads, all the young women had significantly improved their vital capacity (VC), and their maximum oxygen uptake ability was improved to a certain extent. Compared with the 45-minute aerobic exercise group, the vital capacity (VC)of 90-minute aerobic exercise group was significantly increased (P>0.05). Conclusions: When performed at a consistent frequency level, aerobic exercise with a relatively high exercise load can better develop the body's respiratory system function. This may be due to deep stimulation of the respiratory system from high-load aerobic exercise, and ultimately to the intensive exercising of lung function. Level of evidence II; Therapeutic studies - investigation of treatment results.
RESUMO Introdução: O principal objetivo do exercício aeróbico é aumentar a resistência cardiopulmonar, por isso é necessário construir modelos de resposta de resistência cardiopulmonar baseados em diferentes frequências de exercício aeróbico. Objetivo: Estudar a resposta de resistência cardiopulmonar de mulheres em diferentes frequências de exercício aeróbio. Métodos: Vinte jovens profissionais de escritório (professoras e funcionárias públicas) que frequentavam uma academia foram divididas randomicamente em dois grupos. A função cardiopulmonar foi estudada e analisada antes e depois de 16 semanas de exercício aeróbico com diferentes cargas de exercício. Resultados: Depois de 16 semanas de exercícios aeróbicos com diferentes cargas, todas as jovens tiveram melhora significativa da capacidade vital (CV), sendo que a capacidade máxima de captação de oxigênio melhorou até certo ponto. Comparada com o grupo de exercícios aeróbicos de 45 minutos, a capacidade vital (CV) do grupo de exercícios aeróbicos de 90 minutos foi significativamente maior (P > 0,05). Conclusões: Quando praticado com frequência regular, o exercício aeróbico com carga relativamente alta pode melhorar o desenvolvimento da função respiratória. Isso pode dever-se à estimulação profunda do sistema respiratório a partir de exercícios aeróbicos de alta carga e, em última instância,é devidoao exercício intensivo da função pulmonar. Nível de Evidência II; Estudos terapêuticos - Investigação dos resultados do tratamento.
RESUMEN Introducción: El principal objetivo del ejercicio aeróbico es aumentar la resistencia cardiopulmonar, por lo que es necesario desarrollar modelos de respuesta de resistencia cardiopulmonar basados en diferentes frecuencias de ejercicio aeróbico. Objetivo: Estudiar la respuesta de resistencia cardiopulmonar de mujeres a diferentes frecuencias de ejercicio aeróbico. Métodos: Veinte jóvenes profesionales de oficina (profesoras y funcionarias públicas) que asistían a un gimnasio fueron divididas aleatoriamente en dos grupos. Se estudió y analizó la función cardiopulmonar antes y después de 16 semanas de ejercicio aeróbico con diferentes cargas de ejercicio. Resultados: Después de 16 semanas de ejercicio aeróbico con diferentes cargas, todas las mujeres jóvenes presentaron una mejora significativa de la capacidad vital (CV), siendo que la capacidad máxima de captación de oxígeno mejoró en cierta medida. En comparación con el grupo de ejercicio aeróbico de 45 minutos, la capacidad vital (CV) del grupo de ejercicio aeróbico de 90 minutos fue significativamente mayor (P > 0,05). Conclusiones: Cuando se practica con una frecuencia regular, el ejercicio aeróbico con una carga relativamente alta puede mejorar el desarrollo de la función respiratoria. Ello puede deberse a la profunda estimulación del sistema respiratorio por el ejercicio aeróbico de alta carga y, en última instancia, al ejercicio intensivo de la función pulmonar. Nivel de Evidencia II; Estudios terapéuticos - Investigación de los resultados del tratamiento.
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Peptide drugs exhibit an irreplaceable role in clinics due to their high specificity, efficiency and low toxicity. At present, more than 80 peptide drugs have been approved for marketing with global sales exceeding $50 billion in 2019. However, with large molecular weights, high hydrophilicity and instability in digestive tract, oral peptide drugs encounter substantial physiological barriers leading to low oral bioavailability. Therefore, peptide drugs are mostly administered by parenteral routes. Although parenteral delivery of peptide drugs achieves high bioavailability, this is associated with inconvenience and discomfort, even causing severe side effects compared with the oral route possessing a high degree of patient compliance. Therefore, numerous studies concentrate on novel strategies to improve the oral bioavailability of peptide drugs. Some delivery technologies such as Eligen™ and Axcess™ have been successfully applied to the oral dosage form of therapeutic peptides and have accelerated relevant oral formulations for Food and Drug Administration (FDA) approval and clinical treatment. In this review, we focus on the oral peptide delivery, mainly summarizing the progress of recent strategies used to overcome oral barriers and the commercialization applications of related patents, which could facilitate the research and development (R&D) of clinical applications of oral delivery techniques for peptide drugs.
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ABSTRACT Objective: Our aim was to evaluate the association between eHealth literacy and lifestyle behaviors to intervene among Chinese university students. Method: The Chinese eHealth Literacy Scale (C-eHEALS) questionnaire was used to investigate the eHealth literacy level and association with lifestyle behaviors among Chinese university students. Independent sample t-test and Pearson's correlation coefficient were used by statistical software SPSS v20. Results: In the first round, 5,151 university students participated in the study, including 71.46% female and 28.54% male, aged 18-22 (93.13%).The average eHealth literacy score was 26.81 ± 5.83. Four lifestyle behaviors (e.g., exercise, smoking, drinking and sleeping) were all significantly correlated with eHealth literacy scores and demonstrated significant differences. In the second investigation (N = 2,939), the average eHealth literacy score was 31.64 ± 6.44, a notable improvement compared with the first investigation. Conclusion: Those in the group with high eHealth literacy scores have a healthier lifestyle than those in the low-score group. Training in eHealth-related resources is a positive measure to improve university students' eHealth literacy.
RESUMO Objetivo: Nosso objetivo foi avaliar a associação entre alfabetização em saúde e comportamentos de estilo de vida para intervir entre estudantes universitários chineses. Método: O questionário Chinese eHealth Literacy Scale (C-eHEALS) foi usado para investigar o nível de alfabetização em saúde e a associação com comportamentos de estilo de vida entre estudantes universitários chineses. O teste t de amostra independente e o coeficiente de correlação de Pearson foram usados pelo software estatístico SPSS v20. Resultados: Na primeira etapa, participaram 5.151 estudantes universitários, sendo 71,46% do sexo feminino e 28,54% do sexo masculino, com idades entre 18 e 22 anos (93,13%). Quatro comportamentos de estilo de vida (exercícios, fumar, beber e dormir) foram todos significativamente correlacionados com os escores de alfabetização em saúde e demonstraram diferenças significativas. Na segunda investigação (N = 2.939), a pontuação média de alfabetização em saúde foi de 31,64 ± 6,44, uma melhoria notável em comparação com a primeira investigação. Conclusão Aqueles do grupo com pontuações altas de alfabetização em saúde têm um estilo de vida mais saudável que aqueles do grupo com pontuação baixa. A formação em recursos relacionados com a saúde é uma medida positiva para melhorar a alfabetização em saúde dos estudantes universitários.
RESUMEN Objetivo: Evaluar la asociación entre la alfabetización en salud y los comportamientos de estilo de vida para las intervenciones de estudiantes universitarios chinos. Método: Para investigar el nivel de alfabetización en salud y su asociación con los comportamientos de estilo de vida se utilizó el cuestionario Escala de Alfabetización China en Cibermedicina (C-eHEALS). La prueba t de la muestra independiente y el coeficiente de correlación de Pearson se llevaron a cabo con el software estadístico SPSS v20. Resultados: En la primera etapa participaron 5.151 estudiantes universitarios, 71,46% mujeres y 28,54% hombres, con edades comprendidas entre los 18 y los 22 años (93,13%). Cuatro conductas de estilo de vida (ejercicio, fumar, beber y dormir) se correlacionaron significativamente con las puntuaciones de Alfabetización en Salud y mostraron diferencias significativas. En la segunda investigación (N = 2.939), la puntuación media de la alfabetización sanitaria fue de 31,64 ± 6,44, lo que supone una notable mejora en comparación con la primera investigación. Conclusión: Los estudiantes del grupo con puntuación alta de alfabetización en salud tienen un estilo de vida más saludable que las del grupo con puntuación baja. La instrucción sobre recursos relacionados con la salud es una medida positiva para mejorar la alfabetización sanitaria de los estudiantes universitarios.
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Estudiantes , China , Alfabetización en Salud , Estilo de Vida SaludableRESUMEN
Objective@#To investigate the nutritional status of the rural compulsory education students from "Rural Compulsory Education Students Nutrition Improvement Program"(hereinafter referred to "plan") in Ningxia, to provide a scientific basis for future targeted nutrition education and interventions.@*Methods@#The survey was conducted in 27 732 students aged 6 to 15 years selected by stratified samplings in 7 counties of Ningxia.@*Results@#In the 5 years of the "plan" implementation, trend in decreasing malnutrition rates across the 5 years(18.16%, 18.77%, 17.08%, 14.38%, 14.93%), with statistically significant difference(Chi-square for trend was 47.02, P<0.01). Overnutrition rate significantly increased(5.21%, 4.89%, 5.51%, 5.68%, 6.83%, Chi-square for trend was 6.66, P<0.01).@*Conclusion@#Improved nutritional status of rural primary and middle school students in Ningxia has been observed, Co-occurring of malnutrition in boys and overnutrition requires further attention.
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Sanguinarine is the main active component of the Papaver plants, and protopine-6-hydroxylase(P6 H), involved in the sanguinarine biosynthetic pathway, can oxidize protopine to 6-hydroxyprotopine. The investigation on the diversity of P6 H genes in the medicinal Papaver plants contributes to the acquirement of P6 H with high activity to increase the biosynthesis of sanguinarine. Five P6 H genes in P. somniferum, P. orientale, and P. rhoeas were discovered based on the re-sequencing data of the Papaver species, followed by bioinformatics analysis. With the elongation factor 1α(EF-1α), which exhibits stable expression in the root and stem, as the internal reference gene, the transcription levels of P6H genes in roots and stems of the Papaver plants were detected by real-time fluorescent quantitative PCR. As indicated by the re-sequencing results, there were two genotypes of P6H in P. somniferum and P. orientale, respectively, and only one in P. rhoeas. The bioinformatics analysis showed that the P6 H proteins of the three Papaver plants contained the conserved domain cl12078, which is the characteristic of p450 supergene family, and transmembrane regions. The existence of signal peptide remained verification. Real-time fluorescent quantitative PCR results revealed that the transcription level of P6 H in roots of P. somniferum was about 1.44 times of that in stems(α=0.05). The present study confirmed genetic diversity of P6 H in the three medicinal Papaver plants, which lays a basis for the research on the biosynthesis pathway and mechanism of sanguinarine in Papaver species.
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Benzofenantridinas , Alcaloides de Berberina , Sistema Enzimático del Citocromo P-450/genética , Variación Genética , Papaver/genéticaRESUMEN
OBJECTIVE@#To screen the optimal acupuncture regimen for cricopharyngeal achalasia (CPA) after brain stem infarction and compare the therapeutic effect between the combined therapy of catheter balloon dilation and acupuncture and the simple application of catheter balloon dilation.@*METHODS@#The patients suffering from neuropathic dysphagia in CPA after brain stem infarction were selected as the subjects. After confirmed in the diagnosis with video fluoroscopic swallowing study (VFSS), they were randomized into 6 groups, 15 cases in each one, named group A (routine treatment), group B (catheter balloon dilation), group C1 (treated with acupuncture in local area), group C2 (treated with acupuncture based on differentiation), group C3 (treated with acupuncture at the local area and the acupoints based on differentiation) and group D (catheter balloon dilation combined with the optimal acupuncture). Two phases were included in the study. In the first phase of study, the therapeutic effect was compared among the three acupuncture groups, named C1, C2 and C3 group, so as to screen the optimal acupuncture regimen. In the group C1, the main acupoints included Fengchi (GB 20), Wangu (GB 12), Yifeng (TE 17) and three-tongue points (Extra). In the group C2, the main acupoints were Neiguan (PC 6), Tongli (HT 5), Zusanli (ST 36) and Sanyinjiao (SP 6) as well as the supplementary acupoints in accordance with the syndrome differentiation. In the group C1 and group C2, after , the electroacupuncture was used, with continuous dense wave, 5 to 8 Hz in frequency. The needles were retained for 30 min. Acupuncture was given once a day, 5 treatments a week. Before treatment, in 6 weeks of treatment or after removal of gastric tube, the rehabilitation was evaluated. In the group C3, the acupoints, manipulation and treating course were same as the group C1 and group C2. In the 2nd phase of study, theresults of rehabilitation treatment were compared among the group A, group B and group D. The treatment was given once a day, 5 times a week. Before treatment, after gastric tube removal or in 6 weeks of treatment, the evaluation was conducted. The feedingswallowing function grade and VFSS were adopted in the evaluation among the above 6 groups.@*RESULTS@#① In the VFSS comparison at 1st phase of study after treatment, the food transporting ability at oral dysphagia, the results in the group C3 and group C1 were better obviously than the group C2 (both 0.05). The severity of dysphagia in the group C3 was milder than the group C2 and group A (both <0.05). ② In the VFSS comparison at the 2nd phase of study, for the food transporting ability, the results in the group D and the group B were obviously better than the group A (both <0.05). Regarding the function at the pharyngeal dysphagia and aspiration, the results in the group D were better than the group B and group A, those in the group B were better than the group A (all <0.05). The difference in the extubation rate among the group A, group B and group D after treatment was significant statistically (<0.01), of which, the extubation rate in the group D was the highest and the rate in the group A was the lowest. The dysphagia degree in the group D was milder than the group B and group A and that in the group B was milder than the group A (all <0.05).@*CONCLUSION@#In the study of the different acupuncture methods, the acupuncture at the local acupoints and the acupoints selected based on differentiation is the optimal acupuncture regimen for cricopharyngeal achalasia after brain stem infarction. The catheter balloon dilation combined with acupuncture present the synergistc effect on cricopharyngeal achalasia after brain stem infarction, obviously relieve dysphagia and reduce aspiration.
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Humanos , Puntos de Acupuntura , Terapia por Acupuntura , Métodos , Infartos del Tronco Encefálico , Cateterismo , Dilatación , Acalasia del Esófago , Terapéutica , Resultado del TratamientoRESUMEN
Objective: To analyze the microsatellite characteristics and develop microsatellite markers for Sinowilsonia henryi, a rare and endangered plant endemic to China.Method: The Illumina second generation sequencing technology was used to establish the genome library and the microsatellite library of S. henryi. The compositions of the microsatellite were analyzed,and the primers of S. henryi were designed. Its polymorphism was analyzed by the amplification and detection of 5 S. henryi populations.Result: A total of 38,942,and 660 matched sequences of 100 bp were returned by the second generation sequencing. Among them,200,386 splice sequences were obtained by mass pruning and splicing; the number of sequences with length greater than 335 bp was 7 614, and 694 microsatellite loci were detected in the 7 614 sequences,in which the single nucleotide repeats were most and the number of A/T repeated in the single nucleotide repeats was highest. Dinucleotide showed the greatest length variation; the number of AG/CT repeats was highest, and the variation in repeat length was positively correlated with microsatellite abundance. The primers were designed for 36 S. henryi microsatellite sequences with high repetition. By PCR amplification and polyacrylamide gel electrophoresis,20 pairs of primers showed rich polymorphism and clear bands. The number of alleles (NA) ranged from 3 to 6,with average of 4;the polymorphism information content (PIC) ranged from 0.535 5 to 0.754 0,with average of 0.615 5.The population genetic analysis of 5 S. henryi populations showed that the genetic diversity of the species was high (h=0.697 5,I=1.436 8,HE=0.702 2),and the population genetic differentiation was significant (Fst=0.374).Conclusion: The population genetic analysis of S. henryi also showed that the primers developed by this study had a high usability. In this study,the primers of microsatellite markers of S. henryi were established,laying the foundation for the molecular genetics of S. henryi.
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To explore influence of ultrasound‐guided lauromacrogol foam sclerotherapy on peripheral blood levels of counting of endothelial cells (CEC) , endothelin (ET)‐1 and nitric oxide (NO) in patients with sa‐phenous vein varicosis .Methods : A total of 90 patients with saphenous vein varicosis were randomly and equally di‐vided into lauromacrogol group (received ultrasound‐guided lauromacrogol foam sclerotherapy ) and routine treat‐ment group (received routine stripping ).Peripheral blood levels of CEC , ET‐1 and NO were observed and com‐pared between two groups before and after surgery , and therapeutic effect and incidence of complications were eval‐uated.Results : Compared with routine treatment group , there were significant reductions in surgery time [ (81. 79 ± 16.88) min vs .(40.55 ± 10. 19) min] , hospitalization time [(3.94 ± 1.36) d vs.(2. 17 ± 1.31) d] and hospital‐ization fee [ (7640. 15 ± 1025.11) RMB vs.(3998.89 ± 910. 67 ) RMB ] in lauromacrogol group , P＝ 0.001 all. Compared with routine treatment group after surgery , there were significant reductions in incidence rate of total complications (17.78% vs .4. 44%) , percentage of patient＇s condition class IV (17. 78% vs.4.44%) , peripheral blood levels of CEC [ (5562. 48 ± 1194. 73)／L vs.(4655.87 ± 1209. 88)／L] and ET‐1 [ (70. 32 ± 10.30) ng／L vs. (62.95 ± 13.78) ng／L] , and significant rise in percentage of patient’ s condition class I (6.67% vs.25. 00%) and peripheral blood NO level [(1.27 ± 0.42) μmol／L vs.(1. 59 ± 0.51) μmol／L] in lauromacrogol group , P＜0. 05 or＜0. 01. Conclusion : Ultrasound‐ guided lauromacrogol foam sclerotherapy can significantly increase therapeutic effect , improve vascular endothelial function in patients with saphenous vein varicosis .And its complications are few .
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OBJECTIVE@#To establish the STO cell lines expressing green fluorescent protein (GFP) and mouse leukemia inhibitory factor (LIF) , and try to culture the mouse embryonic stem cells (mESCs) by using the established STO-GFP-mLIF cells as the feeder layer.@*METHODS@#The lentiviral particles containing GFP and mLIF and puromycin-resistance gene were constructed and transduced into STO cell lines. The cell lines stably expressing GFP and mLIF genes were screened out. The expression level of the inserted exogenous LIF gene was tested by Western blot and ELISA. The STO-GFP-mLIF cells were treated with different concentrations of mitomycin C (5, 10, 15, 20 µg/ml) for different time (1.5, 2.5, 3, 3.5 hours) to prepare feeder layers and the cell proliferation level on feeder layer was observed. Mouse embryonic stem cells were cultured on mitomycin C-treated feeder layer and the growth of cell colonies was observed.@*RESULTS@#The expression level of LIF protein in STO-GFP-mLIF cells was up-regulated, as compared with STO cells (P＜0.05). It was confirmed that the optimal concentration and time for inhibiting the proliferetion of STO-GFP-mLIF cells by mitomycin C were 10 µg/ml and 3 hours respectively. The observation also found that the embryonic stem cells could develop into typic "birdnest" shaped stem cell colony on mitomycin C-treated feeder layer.@*CONCLUSION@#The stable STO cell lines effectively expressing green fluorescent protein and mouse leukemia inhibitory factor have been established successfully, which can maintain the undifferentiated state of mouse embryonic stem cells.
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Animales , Ratones , Diferenciación Celular , Línea Celular , Separación Celular , Células Madre Embrionarias , Células Nutrientes , Proteínas Fluorescentes Verdes , Factor Inhibidor de LeucemiaRESUMEN
To investigate whether transcription of hepatitis B virus (HBV) gene occurs in human sperm, total RNA was extracted from sperm of patients with chronic HBV infection (test-1), from donor sperm transfected with a plasmid containing the full-length HBV genome (test-2), and from nontransfected donor sperm (control), used as the template for reverse transcription-polymerase chain reaction (RT-PCR). Positive bands for HBV DNA were observed in the test groups but not in the control. Next, to identify the role of host genes in regulating viral gene transcription in sperm, total RNA was extracted from 2-cell embryos derived from hamster oocytes fertilized in vitro by HBV-transfected (test) or nontransfected (control) human sperm and successively subjected to SMART-PCR, suppression subtractive hybridization, T/A cloning, bacterial amplification, microarray hybridization, sequencing and the Basic Local Alignment Search Tool (BLAST) search to isolate differentially expressed genes. Twenty-nine sequences showing significant identity to five human gene families were identified, with chorionic somatomammotropin hormone 2 (CSH2), eukaryotic translation initiation factor 4 gamma 2 (EIF4G2), pterin-4 alpha-carbinolamine dehydratase 2 (PCBD2), pregnancy-specific beta-1-glycoprotein 4 (PSG4) and titin (TTN) selected to represent target genes. Using real-time quantitative RT-PCR (qRT-PCR), when CSH2 and PCBD2 (or EIF4G2, PSG4 and TTN) were silenced by RNA interference, transcriptional levels of HBV s and x genes significantly decreased (or increased) (P < 0.05). Silencing of a control gene in sperm did not significantly change transcription of HBV s and x genes (P > 0.05). This study provides the first experimental evidence that transcription of HBV genes occurs in human sperm and is regulated by host genes.
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Animales , Cricetinae , Humanos , Masculino , Conectina/genética , Factor 4G Eucariótico de Iniciación/genética , Regulación de la Expresión Génica/genética , Silenciador del Gen , Hormona del Crecimiento/genética , Antígenos de Superficie de la Hepatitis B/genética , Virus de la Hepatitis B/genética , Hepatitis B Crónica/virología , Hidroliasas/metabolismo , Glicoproteínas beta 1 Específicas del Embarazo/genética , ARN Viral/análisis , Espermatozoides/virología , Transactivadores/genética , Transcripción Genética , Transfección , Proteínas Reguladoras y Accesorias ViralesRESUMEN
Myocardial bridge is a kind of congenital structural variation, which is usually treated as benign lesions, but it can also cause myocardial ischemia, acute coronary syndrome, ventricular stunning and arrhythmias, even cardiogenic sudden death. The present article made a review on incidence rate, anatomical typing, pathophysiology, clinical manifestations, diagnosis and treatment of myocardial bridge.
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Objective:To explore serum level of high sensitive C reactive protein(hsCRP)and expression of CX3C chemokine receptor 1(CX3CR1)on peripheral blood mononuclear cells(PBMC)in patients with coronary heart disease(CHD)and its clinical significance.Methods:A total of 153 CHD patients treated in our hospital from Mar 2016 to Mar 2017 were selected,including 50 cases with stable angina pectoris(SAP),52 cases with unstable angina pectoris(UAP)and 51 cases with acute myocardial infarction(AMI);according to coronary lesion degree assessed by Gensini score,153 CHD patients were divided into mild coronary disease group(n=43),moderate coronary dis-ease group(n=56)and severe coronary disease group(n=54).Another 45 healthy subjects were selected as healthy control group during the same period.Serum hsCRP level and CX3CR1 expression on PBMC were measured and compared among all groups,the correlation among serum hsCRP level,CX3CR1 expression and Gensini score were analyzed.Results:Compared with healthy control group,there were significant rise in serum hsCRP level[(2.36 ± 1.67)mg/L vs.(5.07 ± 2.16)mg/L vs.(13.59 ± 5.23)mg/L vs.(27.46 ± 8.24)mg/L]and CX3CR1 expression on PBMC[(0.48 ± 0.25)% vs.(11.13 ± 5.42)% vs.(20.56 ± 9.73)% vs.(37.49 ± 12.82)%]in SAP group,UAP group and AMI group,and AMI group> UAP group> SAP group,there was significant difference between any two groups,P=0.001 all.Compared with mild coronary disease group,there were significant rise in serum hsCRP level[(6.31 ± 1.83)mg/L vs.(17.42 ± 6.58)mg/L vs.(35.26 ± 9.74)mg/L]and CX3CR1 expression on PBMC [(6.59 ± 1.43)% vs.(25.86 ± 9.37)% vs.(42.57 ± 13.28)%]in moderate and severe coronary disease group,and those of severe coronary disease group were significantly higher than those of moderate coronary disease group,P=0.001 all.Linear correlation analysis indicated that serum hsCRP level and CX 3CR1 expression on PBMC were sig-nificantly correlated with Gensini score(r=0.768,0.897,P=0.001 both).Conclusion:Serum hsCRP level and CX3CR1 expression significantly rise in CHD patients along with the aggregation of coronary disease,which are ex-pected to be reference indexes predicting severity of CHD.
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Objective To investigate the effects of intravitreal injection of conbercept on diffuse macular edema (DME) of diabetes mellitus.Methods Together 51 diabetic patients (51 eyes) with diffuse macular edema were collected between July 2016 to July 2017,and randomly divided into three groups (n =17):group A,in which patients received solely intravitreal injection of 0.5 mg conbercept,group B,those who was intravitreally injected with 0.5 mg of conbercept and modified macular grating photocoagulation,and group C,patients undergoing modified grid photocoagulation (GPG).Then,before treatment and 1 week,1 month,3 months and 6 months after treatment,all patients were examined by optical coherence tomography for determining central macular thickness (CMT),followed by fundus fluorescein angiography (FFA),the best corrected visual acuity (BCVA),slit lamp,intraocular pressure for compare the efficacy and safety of the three procedures.Results All the three treatments improved BCVA,CMT,retinal neovascularization (RNV) leakage in diffuse DME patients,but group A and B were better than group C (both P ＜ 0.05),while there was no significant difference in BCVA improvement between A and B group (P ＞ 0.05),whereas the improvement in CMT of group B patients was better than that of group A [(197.47 ±45.26) μm vs.(205.59 ± 47.33) μm] (P ＜ 0.001),and this was true of RNV leakage [(9.91 ±3.18)mm vs.(13.24 ±4.87) rnm] (P＜0.001),without related complications.Conclusion Combination of modified macular grid photocoagulation and intravitreal injection of conbercept can quickly relieve diabetic macular edema and alleviate neovascularization,thus improving the patients' vision acuity,of which the efficacy is superior to the pure modified macular grid photocoagulation and conbercept injection.