Study on HPLC Fingerprint of Rosae Rugosae Flos / 中国中医药信息杂志

Objective To establish the HPLC fingerprint of Rosae Rugosae Flos;To provide references for the quality evaluation of Rosae Rugosae Flos.Methods The HPLC analysis was carried on a COSMOSIL 5C18-MS-Ⅱ column(4.6 mm×250 mm,5 μm);the mobile phase was 2.5 % acetonitrile + 0.1 % formic acid aqueous solution(A)-acetonitrile + 0.1 % formic acid(B)with gradient elution at the flow rate of 0.5 Ml/min;the column temperature was 40℃;the detection wavelength was 350 nm.The similarity of 13 batches of samples was evaluated by Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition).Qualitative analysis was carried out by LC-MS technology.The overall quality evaluation of Rosae Rugosae Flos was carried out by combining clustering analysis,principal component analysis and orthogonal partial least square discrimination.Results The common mode of HPLC fingerprints of Rosae Rugosae Flos was established,and the similarity of 13 samples was good.9 compounds were identified preliminary.13 batches of samples were aggregated into 3 categories by chemical pattern recognition.Conclusion The fingerprints of Rosae Rugosae Flos established in this study combines with chemical pattern recognition method,which has high sensitivity and strong specificity,can provide a basis for the quality evaluation of Rosae Rugosae Flos.

Infección simultánea del Injerto Renal por virus BK y CMV: reporte de casos / Simultaneous infection of the renal graft by BK virus and CMV: case report

RESUMEN El camino para llegar a la diálisis peritoneal (DP) como tratamiento de la enfermedad renal crónica (ERC) avanzada estuvo jalonado por hitos a lo largo de la historia. Los conocimientos sobre la anatomía del peritoneo fueron aportados por los embalsamadores egipcios, Galeno (siglo II), y Vesalio (siglo XVI). Recién en 1628 Asellius Gaselli describe los capilares linfáticos abdominales. El siglo XIX fue rico en avances: se identificarn la célula como unidad de los seres vivos y el fenómeno de ósmosis (Dutrochet, 1828), los cristaloides y coloides y su pasaje o no a través de una membrana (Graham T, 1850), el flujo de solutos y partículas a través de la membrana peritoneal (v.Recklinghausen, 1863), la absorción de sustancias hipotónicas y el aumento del efluente con las hipertónicas (Wegner G, 1877), y experimentos en animales confirmaron que la remoción de fluidos y otras sustancias ocurría primariamente a través de vasos sanguíneos (Starling & Tubby, 1894). Pero recién en el siglo 20 se utilizó la DP como tratamiento. El primer intento de utilizar el peritoneo para tratar la uremia lo realizó Georg Ganter en 1923, primero en animales con ligadura de uréteres y luego en dos pacientes. Recién en 1937 se publicó el primer caso que sobrevivió a un "lavaje "peritoneal (Wear y col), pero fueron Fine, Frank y Seligman quienes inicialmente en perros nefrectomizados y luego en pacientes con injuria renal aguda (IRA) demostraron que el método no sólo era viable, sino también efectivo. Luego continuaron los progresos, sobre todo para pacientes con IRA, pero también en algunos casos con ERC avanzada: el doble frasco colgante (Maxwell M, 1959), la diálisis crónica intrahospitalaria con cicladora (Tenckoff y col, 1965), las bolsas plásticas para DP, hasta que en 1975 Moncrief y col pusieron en marcha la DP continua ambulatoria, y en 1981 se introdujo la DP automatizada. Los años noventa fueron de expansión de la DP, hoy instalada como una de las alternativas de tratamiento de la ERC avanzada.

ABSTRACT Milestones throughout history marked the path to reach peritoneal dialysis (PD) as a treatment for advanced chronic kidney disease (CKD). The Egyptian embalmers, Galen (2nd century) and Vesalius (16th century) provided knowledge about the anatomy of the peritoneum. It was not until 1628 that Asellius Gaselli described the abdominal lymphatic capillaries. The 19th century was rich in advances: the cell was identified as the unit of living beings and the phenomenon of osmosis (Dutrochet, 1828), crystalloids and colloids and their passage or not through a membrane (Graham T, 1850), the flow of solutes and particles through the peritoneal membrane (Recklinghausen, 1863), the absorption of hypotonic substances and the increase in effluent with hypertonic ones (Wegner G, 1877), and animal experiments confirmed that fluid removal and other substances occurred primarily through blood vessels (Starling & Tubby, 1894). But it was not until the 20th century that PD was applied as treatment. The first attempt to use the peritoneum to treat uremia was made by Georg Ganter in 1923, first in animals with ureteral ligation and then in two patients. It was not until 1937 that the first case that survived a peritoneal "lavage" was published (Wear et al), but it was Fine, Frank and Seligman who initially in nephrectomized dogs and later in patients with acute kidney injury (ARI) demonstrated that the method was not only viable, but also succesful. Then progress continued, especially for patients with ARI, but also in some cases with advanced CKD: the double hanging bottle (Maxwell M, 1959), chronic intrahospital dialysis with a cycler (Tenckoff et al, 1965), plastic bags for PD, until 1975 when Moncrief et al launched continuous ambulatory PD, and in 1981 automated PD was introduced. The 1990s saw the expansion of PD, to date installed as one of treatment alternatives for advanced CKD.

Surgical strategy and procedure of adrenocortical carcinoma with retrohepatic inferior vena cava tumor thrombus with intraoperative ultrasound guidance / 中华肝胆外科杂志

Adrenocortical carcinoma (ACC) with inferior vena cava thrombosis is rare and has a poor prognosis, and the current literature overwhelmingly supports aggressive surgical intervention. This article summarizes the management of a patient with ACC with inferior vena cava thrombosis, and discusses the feasibility of detailed preoperative imaging data and intraoperative ultrasound to assess the superior and inferior boundaries of ACC with inferior vena cava thrombosis, while describing the intraoperative ultrasound-guided surgical planning and procedure for ACC with retrohepatic inferior vena cava tumor thrombus. Furthermore, it also demonstrates that it is feasible to accurately assess the superior and inferior boundaries of ACC with inferior vena cava thrombosis by preoperative multimodal imaging and intraoperative ultrasound, determine the mode of flow blockage during the operation, and obtain radical resection of the tumor.

Research progress on the effect of mitochondrial and endoplasmic reticulum stress caused by hypoxia during pregnancy on preeclampsia and intrauterine growth restriction / 生理学报

Preeclampsia and intrauterine growth restriction (IUGR) of the fetus are the two most common pregnancy complications worldwide, affecting 5%-10% of pregnant women. Preeclampsia is associated with significantly increased maternal and fetal morbidity and mortality. Hypoxia-induced uteroplacental dysfunction is now recognized as a key pathological factor in preeclampsia and IUGR. Reduced oxygen supply (hypoxia) disrupts mitochondrial and endoplasmic reticulum (ER) function. Hypoxia has been shown to alter mitochondrial reactive oxygen species (ROS) homeostasis and induce ER stress. Hypoxia during pregnancy is associated with excessive production of ROS in the placenta, leading to oxidative stress. Oxidative stress occurs in a number of human diseases, including high blood pressure during pregnancy. Studies have shown that uterine placental tissue/cells in preeclampsia and IUGR show high levels of oxidative stress, which plays an important role in the pathogenesis of both the complications. This review summarizes the role of hypoxia-induced mitochondrial oxidative stress and ER stress in the pathogenesis of preeclampsia/IUGR and discusses the potential therapeutic strategies targeting oxidative stress to treat both the pregnancy complications.

Tube-nesting EP:A new faster method to get more mouse bone marrow cells / 中国免疫学杂志

Objective:To explore a method to obtain more mouse bone marrow cells more quickly.Methods:Take both lower limbs of the same mouse,the bone marrow cells were obtained by either the tube-nesting EP method or the traditional syringe irrigation method,recorded the time and quantity of bone marrow cells,to analyze the proportion of viable cells by flow cytometry,and to detect the proliferation of these bone marrow cells used CCK-8 method.Results:Compared with the traditional syringe irrigation method,the time of obtaining mouse bone marrow cells by tube-nesting EP method was shorter(P<0.000 1).Compared with the traditional syringe irrigation method,the number of bone marrow cells obtained by tube-nesting EP method was more(P<0.05).The proportion of live cells and proliferation ability of bone marrow cells obtained by the two methods were identical.Conclusion:Tube-nesting EP method is a new faster method to get more mouse bone marrow cells.

Knowledge, attitudes and perceptions on the use of telemedicine among adults aged 18-34 in Manila, Philippines during the COVID-19 pandemic

@#<p style="text-align: justify;">Telemedicine has been an emerging method for delivering healthcare services due to the challenges brought by the COVID-19 pandemic. This descriptive quantitative correlational study is aimed at assessing the knowledge, attitudes and perceptions (KAP), and their relationship among adults aged 18-34 without prior experience in the use of telemedicine in Manila, Philippines during the COVID-19 pandemic. The study had 322 eligible respondents who answered an online survey questionnaire of three parts that asked for their socio-demographic profile, KAP on telemedicine. Descriptive statistics, profile analysis and Spearman's rho were utilized as statistical tools. The respondents' knowledge was on an average to low level, and attitude and perception towards telemedicine were both neutral. Results showed no significant difference between the KAP of the respondents when analyzed according to socio-demographic variables. Knowledge and attitude have a weak positive linear relationship while knowledge and perception have a moderate positive linear relationship. A strong positive linear relationship was indicated between attitude and perception. A positive attitude can be attributed to a positive perception towards telemedicine but both do not consequently come from high knowledge levels of it.</p>

Effects of astaxanthin supplementation in fertilization medium and/or culture medium on the fertilization and development of mouse oocytes / 대한생식의학회지

We investigated the effect of supplementing fertilization medium and/or culture medium with astaxanthin (AST) on the two phases of in vitro fertilization: gamete fertilization and embryo development. Methods: Mouse cumulus-oocyte complexes were divided into four groups with 5 µM AST added to the fertilization medium (group 3, n=300), culture medium (group 2, n=300), or both media (group 4, n=290). No AST was added to the control group (group 1, n=300). Results: The fertilization rate was significantly higher (p<0.001) in the groups using AST supplemented fertilization medium (group 3, 79.0%; group 4, 81.4%) than those without AST (group 1, 56.3%; group 2, 52.3%). The blastocyst rate calculated from the two-cell stage was significantly lower (p<0.001) in the groups using AST-supplemented embryo culture medium (group 2, 58.0%; group 4, 62.3%) than in those without AST (group 1, 82.8%; group 3, 79.8%). The blastocyst rate calculated from the number of inseminated oocytes was highest in group 3 (189/300, 63.0%) and lowest in group 2 (91/300, 30.3%) with statistical significance compared to other groups (p<0.001). There were significantly higher numbers of cells in the inner cell mass and trophectoderm, as well as significantly higher total blastocyst cell counts, in group 3 than in the control group. Conclusion: An increased blastocyst formation rate and high-quality blastocysts were found only in the fertilization medium that had been supplemented with AST. In contrast, AST supplementation of the embryo culture medium was found to impair embryo development.

Research Progress of Forensic Diagnosis Approaches of Early Acute Myocardial Infarction / 法医学杂志

The postmortem diagnosis of acute myocardial infarction (AMI), especially the postmortem diagnosis of early AMI that died immediately after onset or within 1 hour, has always been a difficulty in forensic identification. This article reviews the forensic application of diagnosis and analysis methods for AMI postmortem diagnosis including autopsy imaging, histomorphology, immunohisto-chemistry, biochemical marker and molecular biology diagnosis, and explores the feasible scheme of early postmortem diagnosis in AMI.

Caracterização dos atendimentos clínicos realizados pelo Serviço de Atendimento Móvel de Urgência / Characterization of the clinical care provided by the Mobile Emergency Care Service

Objetivo: Caracterizar o perfil dos atendimentos clínicos prestados pelo Serviço de Atendimento Móvel de Urgência (SAMU). Métodos: Estudo descritivo, transversal, não intervencionista, realizado a partir de dados secundários coletados das fichas de regulação do SAMU de um município brasileiro em 2016. Resultados: Das 2.930 ocorrências estudadas, 1.966 foram atendimentos clínicos (67,1%). A incidência desses atendimentos foi maior no sexo feminino (55,2%). A faixa etária mais acometida foi de pessoas com 70 anos ou mais (20,9%). As síndromes álgicas foram as doenças mais incidentes (16,8%), e, dentre elas, a dor abdominal teve destaque, com 35,8%. Com relação ao destino dado à vítima, 60,2% foram encaminhadas ao serviço hospitalar. A Unidade de Suporte Básico foi enviada em 95,0% das ocorrências. A letalidade observada foi de 3,6%. Conclusão: A grande incidência de doenças clínicas e a caracterização epidemiológica do perfil dos atendimentos destacam a importância de direcionar ações preventivas, promoção da saúde e educação da população a respeito da relevância e da área de abrangência do SAMU, a fim de melhorar continuamente o serviço de saúde.

Objective: To characterize the profile of clinical care provided by the Mobile Emergency Care Service (SAMU). Methods: This is a descriptive, cross-sectional, non-interventional study based on secondary data collected from SAMUS's regulation sheets in a Brazilian city in 2016. Results: Of the 2,930 cases studied, 1,966 were clinical care (67.1%). The incidence of these visits was higher in females (55.2%). The most affected age group was people aged 70 years or above (20.9%). Pain syndromes were the most incident diseases (16.8%), with abdominal pain being highlighted with 35.8%. Regarding the direction given to the victim, 60.2% were referred to the hospital. Ninety five percent of occurrences were referred to the Basic Support Units. The observed lethality was 3.6%. Conclusion: The high incidence of medical diseases and the epidemiological characterization of the care profile highlights the importance of actions directed to prevention, health promotion and education of the population regarding the relevance and the area of coverage of SAMU, to improve the health service continuously

Combination of mRNA of Repair-related Genes in Rat Skeletal Muscles for Wound Age Estimation / 法医学杂志

Objective To investigate the estimation of early and mid-term wound age by a combination of four mRNAs, the DNA polymerase delta-interacting protein 3 （POLDIP3） mRNA, regulator of chromosome condensation 1 like （RCC1L） mRNA, proline-rich 5 （PRR5） mRNA, and ribonucleic acid export 1 （RAE1） mRNA in rats skeletal muscles. Methods The model of rat skeletal muscle contusion was established, and then contusion area muscle tissue was extracted 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44 and 48 h after injury. Histomorphological changes during the repair process after rat skeletal muscle contusion were observed. The relative expressions of Poldip3, Rcc1l, Prr5 and Rae1 mRNAs were detected by reverse transcription real-time quantitative polymerase chain reaction （RT-qPCR）. Different stages of wound age were classified by using the expression patterns of four genes at various time points after injury. The accuracy of the results was verified by Fisher discriminant analysis. Results Histomorphological results showed that the repair process after skeletal muscle contusion occurred with the prolonging of time. Through combination of the expression trends of the four kinds of mRNAs, the 48 h after injury could be divided into three periods, 4-12 h, 16-28 h and 32-48 h. The Fisher discrimination method showed that the classification accuracy rates of the three periods were 83.3%, 75.0% and 73.3%, respectively. Conclusion The classification discrimination based on the relative expression of every gene has a higher accuracy, and the accuracy of wound age estimation with combination of mRNA relative expressions is higher than that with a single indicator. By combining with Fisher discrimination method, this method can be used for early and mid-term wound age estimation.

Research progress of IL-33/ST2 signaling pathway in bone metabolism / 口腔疾病防治

@#Interleukin-33(IL-33) is a new member of the interleukin-1 (IL-1) cytokine superfamily. It can activate mast cells, lymphocytes and macrophages to produce Th2 cytokines and plays a very important role in inflammation, infection, and autoimmune disease. The classical signal pathway of IL-33 includes the isotrimer of ST2 and interleukin-1 receptor accessory protein (IL-1 RAcP), which transduces signals into cells. The IL-33/ST2 signaling pathway affects bone metabolism by activating T and B lymphocytes. This article reviews the role of the IL-33/ST2 signaling pathway in bone metabolism. The results of a literature review showed that at present, scholars at home and abroad still dispute the role of IL-33 in bone metabolism. Some scholars believe that IL-33 can inhibit osteoclast formation, and IL-33 has been recently implicated in physiological bone remodeling. However, other scholars believe that IL-33 can promote osteoclast formation and differentiation, which leads to bone absorption. IL-33 and its signaling pathway are involved in bone metabolism of alveolar bone in periodontitis and periapical periodontitis. The specific mechanism remains unclear, and further studies are warranted.

Study on relationship between caffeine intake level and metabolic syndrome and related diseases in Korean adults: 2013 ~ 2016 Korea National Health and Nutrition Examination Survey / 한국영양학회지

PURPOSE: This study examined the relationship between caffeine intake and metabolic syndrome in Korean adults using the 2013 ~ 2016 Korea National Health and Nutrition Examination Survey data (KNHANES). METHODS: The caffeine database (DB) developed by Food and Drug Safety Assessment Agency in 2014 was used to estimate the caffeine consumption. The food and beverage consumption of the 24 hr recall data of 2013 ~ 2016 KNHANES were matched to items in the caffeine DB and the daily caffeine intakes of the individuals were calculated. The sample was limited to non-pregnant healthy adults aged 19 years and older, who were not taking any medication for disease treatment. RESULTS: The average daily caffeine intake was 41.97 mg, and the daily intake of caffeine of 97% of the participants was from coffee, teas, soft drinks, and other beverages. Multivariate analysis showed that the caffeine intake did not affect metabolic syndrome, hypertension, low HDL-cholesterol, and abdominal obesity. Diabetes and hypertriglyceridemia, however, were 0.76 (95% CI: 0.63 ~ 0.93), and 0.87 (95% CI: 0.77 ~ 0.98) in third quintile (Q3), and 0.66 (95% CI: 0.53 ~ 0.82) and 0.83 (95% CI: 0.73 ~ 0.94) in fourth quintile (Q4) compared to Q1, respectively. Therefore, caffeine intake of 3.66 ~ 45.81 mg per day is related to a lower risk of diabetes and hypertriglyceridemia. CONCLUSION: The study showed that adequate caffeine intake (approximately 45 mg) was associated with a lower prevalence of diabetes and hypertriglyceridemia. Therefore, it can be used as a guideline for the adequate level of caffeine intake for maintaining health.

Exploration for clinical teaching mode of evidence-based laboratory medicine in clinical laboratory students / 中华医学教育探索杂志

In order to improve the core competitiveness and the comprehensive ability of laboratory medicine students, we implemented the clinical teaching mode of evidence-based laboratory medicine in the department of Clinical Laboratory Center of Beijing Children's Hospital, Capital Medical University. This study relied on the platform of National Center for Children's Health, which had abundant case resources and excellent expert team. This study started with the clinical problem, summarized the scientific problem, and carried out teaching activities around the scientific problem. By guidance of the teachers, students used evidence-based medicine methods (clinical case information collection, literature reading, interviews with clinical experts and experimental data collection) to solve the clinical problem. Through research cases,evidence-based medicine methods, clinical thinking and scientific thinking were imparted to students, which can help them integrate theoretical knowledge with clinical practice, and improve learning efficiency, training in clinical thinking and scientific research innovation ability, so as to enhance core competitiveness.

Exploration for clinical teaching mode of evidence-based laboratory medicine in clinical laboratory students / 中华医学教育探索杂志

In order to improve the core competitiveness and the comprehensive ability of laboratory medicine students,we implemented the clinical teaching mode of evidence-based laboratory medicine in the department of Clinical Laboratory Center of Beijing Children's Hospital,Capital Medical University.This study relied on the platform of National Center for Children's Health,which had abundant case resources and excellent expert team.This study started with the clinical problem,summarized the scientific problem,and carried out teaching activities around the scientific problem.By guidance of the teachers,students used evidence-based medicine methods(clinical case information collection,literature reading,interviews with clinical experts and experimental data collection)to solve the clinical problem.Through research cases,evidence-based medicine methods,clinical thinking and scientific thinking were imparted to students,which can help them integrate theoretical knowledge with clinical practice,and improve learning efficiency,training in clinical thinking and scientific research innovation ability,so as to enhance core competitiveness.

Effect of San Gen talkh medicine and San Gen granule medicine. on healthy rats immune system / Монголын эм зүй, эм судлал

Purpose@#To study effect of San Gen talkh medicine and San Gen granule medicine, on healthy rats immune system@*Method@#In this study 32 male healthy Wistar rats were randomly divided into normal group, low dose group of San Gen talkh (SGTLD), high dose group of San gen talkh (SGTHD) and group of San Gen granule (SGG). In normal group rats were administrated 0.2m l/kg saline solution, in San gen talkh groups rats with low dose and high dose were administrated 0.85g/kg, 1.70g/kg San Gen talkh respectively and in San gen granule medicine groups rats 1.3 g/kg San gen granule medicine, once daily for 42 day. By Elisa method measured IL-10, IL-12, IL-17, IFN-γ and TGF-β in blood plasma and spleen tissue of rat.@*Result@#In this experimental result, there was no significantly difference all measured cytokine of blood plasm (P>0.05) between healthy group and all medicine group. In spleen tissue, there was significantly increased IFN-γ (P<0.05) and TGF-β (P<0.05) in SGTHD group and was significantly increased IFN-γ (PO.05), IL-10 (P<0.01)and TGF-β (PO.001) in SGG group compared with normal group.@*Conclusion@#High doses San Gen talkh medicine and San Gen granule medicine can support cellular immune system, humoral immune system on healthy rats.

Serviço de Atendimento Móvel de Urgência: perfil dos atendimentos por causas externas / Emergency Mobile Care Service (SAMU): profile of attendance due to external causes

OBJETIVO: Descrever os atendimentos por causas externas realizados pelo serviço de atendimento pré-hospitalar prestado pelo Serviço de Atendimento Móvel de Urgência (SAMU). MÉ- TODOS: Estudo de corte transversal, descritivo, observacional, de abordagem quantitativa, tendo como fonte dados Fichas de Regulação do SAMU de Governador Valadares, Minas Gerais, preenchidas no período de 1° de janeiro de 2016 a 31 de dezembro de 2016. RESULTADOS: Foram analisadas 2.930 fichas; destas 964 (32,9%) eram referentes a ocorrências por causas externas. Os agravos mais frequentes foram, em ordem decrescente: acidentes de trânsito, queda da própria altura, queda de altura, agressões e outros. Eram homens 64,5% e a idade média foi de 38,4 anos (zero a 99 anos de idade). A maioria dos agravos se deu durante 12h01 e 18h, e, em 94,8% dos atendimentos, foi enviada uma Unidade de Suporte Básico. Dentre as vítimas, 85,58% foram entregues ao hospital. A letalidade foi de 1,35%. CONCLUSÃO: Governador Valadares recebe diariamente elevada demanda de atendimentos pré-hospitalares por causas externas de diversos pontos da cidade, traduzindo a necessidade de alternativas especializadas e eficazes para os atendimentos. Cabe à atuação do serviço disponibilizar um atendimento precoce, de forma a reduzir o tempo de atendimento, o que resulta em melhor prognóstico dos agravos. (AU)

OBJECTIVE: To describe assistance due to external causes by the pre-hospital care service provided by the Emergency Mobile Care Service (SAMU). METHODS: This is a cross-sectional, descriptive, observational, quantitative study with data obtained from the Regulation Sheets of the Emergency Mobile Care Service of Governador Valadares, Minas Gerais, completed in the period from January 1, 2016 to December 31, 2016. RESULTS: A total of 2,930 records were analyzed, of which 964 (32.9%) were related to occurrences due to external causes. The most frequent problems were, in descending order: traffic accidents, fall from standing, fall from heights, aggressions, and others. Men represented 64.5%, and the mean age was 38.4 years (0 - 99 years old). Most injuries occurred between 12:01 and 6:00 p.m., and in 94.8% of the assistance, a Basic Support Unit was sent. Of the victims, 85.58% were taken to hospital. Lethality was 1.35%. CONCLUSION: Governador Valadares daily receives a large demand for prehospital care due to external causes in several parts of the city, reflecting the need for specialized and effective alternatives for care. It is up to the service to provide early care, to reduce the time of assistance, which results in a better prognosis of the diseases. (AU)

Homogeneity of Different Functional mRNA Indicators for Wound Age Estimation / 法医学杂志

OBJECTIVES@#To explore the homogeneity level of four different functional mRNA （PUM2, TAB2, Cx45 and CHRNA1） expressions in rats with skeletal muscle contusion.@*METHODS@#The relative expressions of PUM2, TAB2, Cx45 and CHRNA1 mRNAs were detected by quantitative real-time reverse transcription-polymerase chain reaction （RT-qPCR）. The coefficient of variation （CV） of the relative expressions for different individuals in each injury group was calculated. The extreme value of CV, cumulative variability, and CVCV were compared.@*RESULTS@#A high CV of PUM2 and TAB2 mRNAs appeared on several different time points. However, the CV of Cx45 and CHRNA1 mRNAs was relatively low. The cumulative variability from high to low was PUM2, CHRNA1, TAB2 and Cx45 mRNAs. The relative expression of PUM2 mRNA was significantly higher than that of TAB2, Cx45 and CHRNA1 mRNAs （ P<0.05）. There was no statistical significance （P>0.05） in the CVCV of the relative expression of TAB2, CHRNA1 and Cx45 mRNAs.@*CONCLUSIONS@#As the mRNAs involving in biological process regulation, PUM2 and CHRNA1 mRNAs show a lowest individual homogeneity of the relative expression followed by TAB2 mRNA. As the mRNAs participating in the composition of cellular structure, Cx45 and CHRNA1 mRNAs show a high individual homogeneity of the relative expressions. The functional classification should be considered for the screening of the mRNA indicators used for wound age estimation.

Study on the Quality Standard of Chinese Mongolian Medicinal Materials Venerates / 世界科学技术-中医药现代化

This paper aimed at studying the physical and chemical properties, general examination and contents of someeffective components of Erratum Mongolia, and to provide scientific basis for the establishment of the quality standard ofvenerates Mongolia. The physical and chemical properties of venerates were identified by microscope and thin layerchromatography, and the general examination of venerates was studied with reference to the appendix of the 2010 editionof People's Republic of China Pharmacopoeia, and the main characteristic components and the active components inErratum were analyzed by HPLC. The content was analyzed and compared. The characters and microscopic identificationconfirmed that all the 7 batches of samples were venerates. The TLC identification was used as the control product ofhermitage, Erratum and restorative. The test products showed the same color spots in the corresponding position of thecontrol products. The spots were clear and the separation degree was good; the water and extracts of the different batch ofvenerates were examined by general terms. The content of ash in the Erratum collected from different regions was notsignificantly different (5.74-6.98％) . The content of the main active ingredients (hermitage) in Erratum from differentareas of the same plant is compared for the first time. The method is simple and reproducible. It provides a scientificbasis for improving the quality standard of Erratum Mongolia, the safe and rational application and the furtherdevelopment and production.

Bioinformatics analysis and fluorescence quantitative PCR results of Ricinus communis PIP5Ks / 中草药

Objective To predict the expression of seven genes in the PIP5K gene family, and to determine the relative expression of PIP5Ks in different stages of type Lm gynoecious Ricinus communis aLmAB2. Method Bioinformatics analysis of online software protein physicochemical properties analysis, protein hydrophobicity analysis subcellular localization prediction, transmembrane region analysis, etc. Bioinformatics analysis of hemp PIP5K gene family and DNAMAN for sequence alignment and real-time PCR. Results The results showed that there were seven members in the R. communis PIP5Ks, which were PIP5K1, PIP5K2, PIP5K4, PIP5K6, PIP5K8, PIP5K9, and PIP5K11. The amino acid sequence homology of the corresponding protein of R. communis PIP5K reached 48.06%, and the protein of R. communis PIP5Ks all are hydrophilic proteins. Except that PIP5K9 and PIP5K11 were unstable proteins, the rest were stable proteins, and the corresponding proteins of PIP5Ks have no transmembrane domain, and all were non-transmembrane proteins. Subcellular localization results showed that the proteins PIP5K1, PIP5K4, PIP5K6, PIP5K9, and PIP5K11 were less likely to have a lead peptide, and there was no corresponding amino acid cleavage site, ie, localized in other organelles or possibly cytoplasmic proteins; Protein PIP5K2 was localized in chloroplasts, the chloroplast transit peptide has a higher value, and the protein PIP5K8 was located in the secretory pathway. From the perspective of the relative expression of PIP5Ks in the differential expression, except that no fluorescence was detected in PIP5K4, the other PIP5Ks had a certain differential expression. The changes of relative expression of PIP5Ks showed similar trends, the levels of PIP5K1, PIP5K2 relative expression were high, followed by the relative expression of PIP5K8, PIP5K9, and PIP5K6 and PIP5K11 only have fewer expressions. Conclusion It is concluded that PIP5Ks may have a certain effect on the inflorescence axis traits in ramie. The differential expression of PIP5K on the inflorescence axis has the certain regularity with the development of inflorescence.

Research summary of Mongolian medicine Trogopterus xanthipes Milne-Edwards / Монголын эм зүй, эм судлал

Background@#Trogopterus xanthipes Milne-Edwards (Hadan hailmal) is defecation of Olbo mouse with red and yellow foot in Olbo mouse family. Trogopterus xanthipes Milne-Edwards has bitter and little sweety flavor and cool and sharp quality and it has effects such as to remove hot, to stop pain and to to make urinate etc.@*Purpose@#To summarize chemical compounds, pharmacological effects, clinical usage and ancient source notes of Trogopterus xanthipes Milne-Edwards. @*Method@#Вy analys method to analyzed research articlest and ancient source of Trogopterus xanthipes Milne-Edwards in China Biological Medical Digest Data-base, Chinese Journals Full Text Data-base and ancient sources of Mongolian traditional medicine.@*Results and Conclusions@#Trogopterus xanthipes Milne-Edwards is produced juice from gold, silver, copper, iron, lead and tin on cliff during 4 to 6 months and otherwise Trogopterus xanthipes Milne-Edwards is called defecation of olbo mouse. It has many activity chemical compounds, such as nitrogen compound, volatile compound, Triterpenoid, fatty acid, flavone, and microelement and has many pharmacological effects such as anti gastric ulcer, anti inflammation, anti oxidant, anti cerebral ischemic injury, anti cytotoxicity and to improve immunology. In Mongolian traditional medicine, Trogopterus xanthipes Milne-Edwards mainly used to treat hot disease of stomach, liver and kidney, synovial disease, gout and rheumatism etc with single medicine and compound recipe ingredients of Trogopterus xanthipes Milne-Edwards. In Chinese medicine used to treat disease of digestive system and to stop pain etc.