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【Objective】 To investigate whether the current retention methods in blood stations can fully meet the traceability requirements of blood test results by analyzing the traceability of retained samples for syphilis antibody testing. 【Methods】 Thirty-four one-assay-positive deep-well plate retention samples, 68 double-assay-positive deep-well plate retention samples and 263 negative retention blood braids and corresponding deep-well plate retention samples that expired retention period for syphilis antibody testing from 2014 to 2020 in our center were collected. The TP-ELISA assays of two manufacturers were used for retesting, and the results were recorded and compared with the original results statistically. 【Results】 The concordance rate of the double-assay-positive and single-assay-positive samples with their corresponding deep-well plate samples was 98.53%(67/68) and 67.65%(23/34), respectively(P<0.05). Specific results for single-assay-positive syphilis antibody samples and their corresponding deep-well plate retention samples were as follows: 1) Single positive (reagent A): 13 out of 14 original samples were 0.65
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【Objective】 To establish a reasonable and effective blood screening strategy for Hepatitis C virus (HCV), so as to reduce the risk of blood transfusion transmission, ensure blood safety and improve the quality of blood screening. 【Methods】 In order to evaluate HCV screening strategies comprehensively, the unqualified blood donations due to anti-HCV alone positivity in Dalian from 2017 to 2021 was tracked, with combined detection methods of electro-chemiluminescence immunoassay (ECLIA) and HCV-RNA nucleic acid test (NAT). 【Results】 A total of 851 (0.20%) unqualified donations due to anti-HCV alone positivity were screened from 2017 to 2021, with a decreasing trend in both numbers and rate. Among them, the unqualified rate of samples with anti-HCV reactivity in both dural-ELISA-reagent and NAT decreased significantly (P<0.05). A total of 117(0.028%) samples were anti-HCV reactive in dural-ELISA-reagent but nonreactive in NAT; 664 reactive in one-ELISA-reagent, with 70(10.54%) in Reagent Ⅰ and 594(89.46%) in Reagent Ⅱ; 122 (35.88%) out of 340 donations were reactive in ECLIA. Among the 28 participants in the follow-up test, 15 still were reactive in ELISA and 2 reactive in ECLIA. 【Conclusion】 Although the unqualified rate of HCV is decreasing, serological screening of anti-HCV is still an important method for ensuring blood safety, and its complementarity with HCV-RNA NAT should be evaluated. As a new serological assay, ECLIA has high sensitivity and specificity. Miss detection may occur if only one ELISA reagent is adopted for anti-HCV detection. Appropriate ELISA and NAT system for HCV screening should be reasonably chosen, and HCV screening strategy should be developed and adjusted according to the local conditions.
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【Objective】 To improve the efficiency and screening strategy by evaluating the performance of HBsAg screening before blood donation in Dalian. 【Methods】 The confirmed HBsAg positive samples were detected qualitatively and quantitatively, and retested with HBsAg rapid testing kit pre-donational used parallelly. Efficiency of HBsAg screening before donation was calculated, and compared between first-time and repeated donors. 【Results】 The pre-donation HBsAg screening was conducted for 199 553 cases, including 95 321 first-time donors (63.2%) and 55 396 repeated donors(36.8%).1 015 donors (first-time/repeated: 999/16) were reactive in HBsAg rapid testing. 113 samples (first-time/repeated: 108/5), initially negative in HBsAg rapid testing, were confirmed HBsAg positive by laboratory testing. The efficiency of pre-donation HBsAg screening for first-time donors(10.9‰) was significantly higher than that of repeated donors(0.3‰)(P130) and 92.44 (18.7->130), respectively. 【Conclusion】 The HBsAg rapid testing kit used in Dalian Blood Center could meet requirements for blood donor screening before donation, while good efficiency of HBsAg pre-donation screening could be achieved only in first-time blood donors.
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Objective Based on data related to arsenic contents in paddy rice,as part of the food safety monitoring programs in 2017,to discuss and explore the application of spatial analysis used for food safety risk assessment.Methods One province was chosen to study the spatial visualization,spatial point model estimation,and kernel density estimation.Moran's I statistic of spatial autocorrelation methods was used to analyze the spatial distribution at the county level.Results Data concerning the spatial point model estimation showed that the spatial distribution of pollution appeared relatively dispersive.From the kernel density estimation,we found that the hot spots of pollution were mainly located in the central and eastern regions.The global Moran's I values appeared as 0.11 which presented low spatial aggregation to the rice arsenic contamination and with statistically significant differences.One "high-high" and two typical "low-low" clustering were seen in this study.Conclusion Results from our study provided good visual demonstration,identification of pollution distribution rules,hot spots and aggregation areas for research on the distribution of food pollutants.Spatial statistics can provide technical support for the implementation of issue-based monitoring programs.
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Objective: Based on data related to arsenic contents in paddy rice, as part of the food safety monitoring programs in 2017, to discuss and explore the application of spatial analysis used for food safety risk assessment. Methods: One province was chosen to study the spatial visualization, spatial point model estimation, and kernel density estimation. Moran's I statistic of spatial autocorrelation methods was used to analyze the spatial distribution at the county level. Results: Data concerning the spatial point model estimation showed that the spatial distribution of pollution appeared relatively dispersive. From the kernel density estimation, we found that the hot spots of pollution were mainly located in the central and eastern regions. The global Moran's I values appeared as 0.11 which presented low spatial aggregation to the rice arsenic contamination and with statistically significant differences. One "high-high" and two typical "low-low" clustering were seen in this study. Conclusion: Results from our study provided good visual demonstration, identification of pollution distribution rules, hot spots and aggregation areas for research on the distribution of food pollutants. Spatial statistics can provide technical support for the implementation of issue-based monitoring programs.
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Humanos , Arsénico/efectos adversos , China , Análisis por Conglomerados , Contaminación de Alimentos , Abastecimiento de Alimentos , Análisis EspacialRESUMEN
Objective To prolong the survival time of patients after gastrectomy for gastric cancer as much as possible, multivariate analysis was performed to investigate the risk factors of prognosis. Methods 81 cases of postoperative gastric cancer patients who had been treated in our hospital from January 2011 to December 2013 were selected as subjects using random number table. The clinical data of the enrolled patients were retrospectively analyzed. Factors that affecting survival time were investigated using multivariable analysis. Results Univariate analysis showed that the risk factors affecting the 5-year survival rate of patients undergoing gastrectomy included postoperative chemotherapy, tumor differentiation, tumor infiltration, tumor volume, lymph node metastasis, and TNM stages (P<0. 05). Multivariate Logistic regression analysis revealed that tumor differentiation, lymph node metastasis, and tumor TNM stages were independent factors that influenced the 5-year survival rate of patients after gastrectomy. Conclusion The survival time of patients after gastrectomy is affected by many factors, among which the degree of tumor differentiation, lymph node metastasis, and the TNM stage of tumors are independent factors influencing the prognosis of patients, which is worth clinical attention.
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<p><b>OBJECTIVE</b>An ion chromatographic method was developed to analyze sulfur dioxide in traditional Chinese medicine (TCM).</p><p><b>METHOD</b>The samples were extracted with alkaline solution and reacted with formaldehyde to form hydroxymethyl sulfonic acid. The extracts were cleaned by C18 column and SO3(2-) was determined with ion chromatography equipped with a conductance detector. In the method, AS11-HC chromatographic column was used and the mobile phase was 15 mmol x L(-1) KOH.</p><p><b>RESULT</b>The calibration curve was in good linearity in the range of 1 to 100 microg (r =0.9992). The detection limit was 0.53 mg x kg(-1) and the average recovery was 103.7%.</p><p><b>CONCLUSION</b>The method is simple, sensitive, precise, short-time-consuming, and the random error is smaller, which is applicable to the testing of large quantity batches of TCM.</p>
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Álcalis , Química , Cromatografía por Intercambio Iónico , Métodos , Contaminación de Medicamentos , Medicamentos Herbarios Chinos , Plantas Medicinales , Química , Control de Calidad , Dióxido de AzufreRESUMEN
Objective:Mutations in the CX(connexin)26 gene (GJB2), encoding the gap\|junction protein. Connexin 26, have been shown to be the major cause of non\|syndromic recessive deafness. Among these mutations, the CX 26 cDNA (35delG) mutation accounts for the majority of this kind of deafness are European and America, but it does not present in China. Therefore, we intend to investigate the mutation hot spot of connexin 26 in China deafness populations. Mathods:The mutation of 233delC was detected by the technique of PCR\|RFLP and analyzed by directly sequencing protocols in 219 patients with kinds of deafness and 50 individuals with normal hearing. Results: The frequency of the 233delC mutation was 47/219(21.5%) in the Chinese population. No mutation was found in group with normal hearing and dominant nonsyndromic hearing loss. The mutation frequency for 233delC was 4 in 15 patients (26.7%) with genetic prelingual deafness and 10 in 50 patients(20.0%) with drug\|deafness. Conclusion: The 233delC mutaion had a high frequency in Chinese population with hearing loss, especially congenital deafness. Our data indicated that specific combinations of GJB2 mutation exist in different populations.
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Objective To investigate the serum high-mobility group box protein 1(HMGB1)level in sepsis patients with different syndrome patterns,and to explore the correlation of HMGB1 level with defense-Qi-nutrient-blood syndrome differentiation and Acute Physiological and Chronic Health Evaluation Ⅱ(APACHEⅡ)score.Methods Twenty-four sepsis patients were differentiated as Qi system syndrome(QS group,N=11)and nutrient-blood system syndrome(NB group,N=13).Eight healthy volunteers served as the normal control.APACHEⅡ score was evaluated,serum HMGB1 level was detected by Western blot method,and the correlation of HMGB1 level with defense-Qi-nutrient-blood syndrome differentiation and APACHEⅡ score was analyzed.Results APACHEⅡ score was higher in NB group than that in QS group,the difference being significant(P
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Objective:To study the expression of Survivin,VEGF,p53 and their correlation inbreast cancer, 22 cases of hyperplasia of mammary gland and 10 cases of paracainoma normal tissues by immunohistochemical technique (SP).Results: The positive rates of Survivin were 76.7%,18.2% and 0% in breast cancer, hyperplasia of mammary gland and paracacinoma normal tissues (P
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AIM:To establish a method for detecting three aconitum alkaloids(aconitine,mesaconitine,hy-paconitine) in Zhentong Huoluo Tincture(Radix Aconiti,Radix Aconiti kusnezoffii,Radix et Rhizoma Rhei,Rhizoma Pinelliae,Rhizoma Arisaematis ect.).METHODS:A ZOBAX Extend-C_ 18(250 mm?4.6 mm,5 ?m) column was used to determine the aconitum alkaloids in Zhentong Huoluo Tincture.The mobile was gradient elution,0.1% die-thylamine(adjust pH 9.0 with phosphoric acid) as mobile A;0-20 min A∶B(63∶37),20-45 min A∶B(63∶55∶37→45),at the rate of 1 mL/min.The temperature of column was 30 ℃,and the wavelength was at 232 nm.RESULTS:The linearity of the aconitine in 9.74 ng-974 ng was 0.999 9;that of the mesaconitine in 23.86-2 386 ng was 0.999 9 and that of the hypaconitine in 29.94 ng-2 994 ng was 0.999 9.The RSD of precision for these three were 1.50%、0.68% and 0.49%,respectively.The average recoveries and its RSD were 100.2%(RSD 3.2 %)、102.9%(RSD 2.2%) and 98.2(RSD 2.0%),respectively for aconitin,mesaconitine and hypaconitine.The reproducibilities were all less than 2.0%,and the sample solution was stable in 24 h.CONCLUSION:The method is simple,accurate and has good stability,can be used for the quantity control in the product of Zhentong Huoluo Tincture.