RESUMEN
Objective: To establish an HPLC-ELSD method for simultaneous determination of astragaloside IV, ferulic acid, paeoniflorin, glycyrrhizic acid, vaccarin, naringin, neohesperidin, and platycodin D in Buxue Shengru Granules (BSG). Methods: The analysis of methanol extract of this drug was performed on a Diamonsil C18 (250 mm × 4.6 mm, 5 μm) with the mobile phase comprising of 0.1% formic acid aqueous solution-acetonitrile. Flow rate was 1 mL/min and column temperature was 30 ℃. The samples were tested on Evaporative Light-scattering Detector with drift tube temperature of 40 oC. Results: The analysis permitted very good separation of eight constituents within 52 min. The linearity ranges of the eight constituents were 62.5-1 250.0 μg/mL (r > 0.995 0). The average recoveries of eight constituents in the samples were in the range of 95.51%-99.47%, and the RSD ranged from 0.31% to 3.70%. Intraday and interday precisions RSD of the peak areas of the eight components were less than 3%; The repeatability RSD of each component ranged from 0.94% to 2.11%; Eight components had good stability within 18 h, and the concentration RSD of each component ranged from 0.98% to 2.86%. The content of vaccarin, paeoniflorin, ferulic acid, naringin, neohesperidin, platycodin D, astragaloside IV, and glycyrrhizic acid were 0.550 7-0.584 3, 19.657 9-19.952 1, 0.350 5-0.384 7, 18.794 7-19.557 3, 12.124 7-12.414 2, 0.610 7-0.631 3, 0.238 8-0.274 3, and 2.750 4-2.852 2 mg/g, respectively. Conclusion: This simple and accurate method can be used for the rapid quality control of BSG.
RESUMEN
AIM To investigate the toxicity of Buxue Shengru Granules (BSG,Astragali Radix,Angelicae sinensis Radix,Paeoniae Radix alba,etc.) to perinatal SD rats and their offspring to determine its safety.METHODS The respective BSG administration of 0,1,3,10 g/kg to the four groups rats,each with twenty-four female rats,started from the day 15 of gestation (GD15) through weaning when they were procured for the identification of the general condition,body weight and food ingestion.The offspring had their postnatal appearance observed the physiological development,reflexes development,behavior and fertility detected as well.RESULTS BSG gave neither significant influence on the rats' general condition,time of birth,body weight,and histological changes of organs during perinatal period;nor remarkable impact on the offspring's growth,development,nerves,endocrine,and reproductive system.CONCLUSION Data on BSG to rats maternal weight gain and food intake and to their offspring developmental landmarks,sexual maturation,or reflexes suggest that BSG gives no perinatal toxicity.