RESUMEN
Foot and mouth disease is a highly contagious viral disease that poses a significant economic threat to cloven-hoofed animals, including cattle and sheep. The emergence of a novel foot and mouth disease virus-A isolate, FMDV-A-Egy-AHRI-RL385-Ven-2022, in Egypt in 2022 has raised concerns about its potential impact on existing vaccination programs. Given that vaccination is a key strategy for foot and mouth disease virus control, the present study was aimed to assess the cross-protective efficacy of both local and imported inactivated vaccines against this new threat. Through challenge experiments and serum neutralization tests, we observed limited effectiveness of both vaccine types. The calculated r1-values at 28 days post-vaccination indicated a minimal immune response to FMDV-A-Egy-AHRI-RL385-Ven-2022 (0.176 and 0.175 for local and imported vaccines, respectively). Challenge experiments further confirmed these findings, revealing 0percent protection from the local vaccine and only 20percent rotection from imported vaccines by day 7 post-challenge. These results underscore the urgent need to update existing foot and mouth disease virus vaccines in Egypt by incorporating the newly circulating FMDV-A-Egy-AHRI-RL385-Ven-2022 strain. This proactive measure is crucial to prevent future outbreaks and ensure effective disease control(AU)
La fiebre aftosa es una enfermedad vírica muy contagiosa que supone una importante amenaza económica para los animales biungulados, entre ellos el ganado vacuno y ovino. La aparición de un nuevo aislado del virus A de la fiebre aftosa, el FMDV-A-Egy-AHRI-RL385-Ven-2022, en Egipto en 2022 ha suscitado preocupación por su posible impacto en los programas de vacunación existentes. Dado que la vacunación es una estrategia clave para el control del virus de la fiebre aftosa, el objetivo del presente estudio fue evaluar la eficacia protectora cruzada de las vacunas inactivadas locales e importadas frente a esta nueva amenaza. Mediante experimentos de desafío y pruebas de seroneutralización, observamos una eficacia limitada de ambos tipos de vacuna. Los valores r1 calculados a los 28 días posvacunación indicaron una respuesta inmunitaria mínima frente a FMDV-A-Egy-AHRI-RL385-Ven-2022 (0,176 y 0,175 para las vacunas local e importada, respectivamente). Los experimentos de provocación confirmaron aún más estos resultados, revelando un 0 por ciento de protección de la vacuna local y sólo un 20 por ciento de protección de las vacunas importadas al séptimo día después de la provocación. Estos resultados subrayan la urgente necesidad de actualizar las vacunas existentes contra el virus de la fiebre aftosa en Egipto incorporando la nueva cepa circulante FMDV-A-Egy-AHRI-RL385-Ven-2022. Esta medida proactiva es crucial para prevenir futuros brotes y garantizar un control eficaz de la enfermedad(AU)
Asunto(s)
Animales , Brotes de Enfermedades , Ganado , Fiebre Aftosa/epidemiología , Vacunas , EgiptoRESUMEN
A bovinocultura possui grande importância na economia brasileira, entretanto, enfermidades afetam a produção, ocasionando perdas econômicas, como a miíase. O agente causador da miíase é Cochliomyia hominivorax, mosca-da-bicheira. Os agentes são aptos às regiões tropicais e subtropicais no Brasil, e exibem resistência parasitária aos fármacos. O objetivo deste trabalho foi verificar a ocorrência de C. hominivorax em bovinos no estado do Maranhão, Brasil. Foram escolhidas 19 propriedades de bovinos leiteiros e de corte, distribuídas em 11 municípios maranhenses. Foi indagado, por questionário, o manejo, os medicamentos utilizados e o custo de prevenção relacionado à mazela. Os bovinos foram examinados por inspeção, recolhendo-se parcialmente as larvas encontradas na superfície corporal. O número de larvas por bovino, local das lesões, a origem e complicações foram observadas e anotadas. Em seguida, os espécimes coletados foram acondicionados em frascos individuais, por hospedeiro amostrado, contendo álcool a 70°Gay-Lussac (GL). No laboratório as larvas das moscas foram examinadas em estereomicroscópio e identificadas pela sua morfologia. Verificou-se que mais de 60% (12/19) das propriedades possuíam bovinos parasitados. Dentre os 4.509 animais observados, 0,57% (26/4509) estavam parasitados com as larvas de C. hominivorax. Encontraram-se infestações no umbigo (50%), bem como na tábua do pescoço (42%), vulva (4%) e chifre (4%). Conclui-se que houve uma baixa prevalência de miíase causada por C. hominivorax em bovinos no estado do Maranhão, porém a prevenção e cuidados sanitários sempre devem ser adotados pelos pecuaristas.
Cattle breeding has great importance in the Brazilian economy, however, diseases affect production, causing economic losses, such as myiasis. The causative agent of myiasis is Cochliomyia hominivorax, the screwworm fly. The agents are suitable for tropical and subtropical regions in Brazil, and exhibit parasitic drug resistance. The objective of this work was to verify the occurrence of C. hominivorax in cattle in the state of Maranhão, Brazil. In the first stage, 19 dairy and beef cattle properties were chosen, distributed in 11 municipalities in Maranhão. Questionnaires asked about the management, the drugs used and the cost of prevention related to the disease. The cattle were examined by inspection, partially collecting the larvae found on the body surface. The number of larvae per bovine, location of lesions, origin and complications were observed and noted. Then, the collected specimens were placed in individual flasks, per sampled host, containing alcohol at 70°Gay-Lussac (GL). In the second stage, the fly larvae were identified in the laboratory, examined under a stereomicroscope and identified by their morphology and analysis of perithematics plaques and respiratory spiracles. It was found more that 60% (12/19) of the properties had parasitized cattle. Among the 4.509 animals observed, 0.57% (26/4509) were parasitized with the larvae of C. hominivorax. Infestations were found in the navel (50%), as well as in the neck plate (42%), vulva (4%) and horn (4%). It is concluded that there was a low prevalence of myiasis caused by C. hominivorax in cattle in the state of Maranhão, but prevention and health care should always be adopted by livestock farmers.
Asunto(s)
Animales , Bovinos , Infección por Gusano Barrenador/veterinaria , Enfermedades de los Bovinos/parasitología , Prevalencia , Ganado/parasitología , Carga de Parásitos/veterinaria , Larva/parasitología , Miasis/veterinariaRESUMEN
CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein) is widely used in the field of livestock breeding. However, its low efficiency, untargeted cutting and low safety have greatly hampered its use for introducing single base mutations in livestock breeding. Single base editing, as a new gene editing tool, can directly replace bases without introducing double strand breaks. Single base editing shows high efficiency and strong specificity, and provides a simpler and more effective method for precise gene modification in livestock breeding. This paper introduces the principle and development of single base editing technology and its application in livestock breeding.
Asunto(s)
Animales , Edición Génica , Sistemas CRISPR-Cas/genética , Ganado/genética , Mutación , TecnologíaRESUMEN
El virus de la fiebre aftosa es un patógeno altamente infeccioso y contagioso. Recientemente, el topotipo VII, linaje Lib-12 del serotipo SAT2 se describió en brotes en Egipto durante 2018. La vacunación es una forma eficaz de controlar y combatir los brotes del virus de la fiebre aftosa, especialmente en áreas endémicas como Egipto. El presente estudio tuvo como objetivo evaluar la eficacia de la vacuna contra la fiebre aftosa que se produce actualmente, frente a la cepa de campo recientemente aislada del virus de la fiebre aftosa SAT2 topotipo VII, linaje Lib-12 (SAT2 Libia), mediante la aplicación de estudios in vitro e in vivo. Se inocularon en terneros, dos lotes de la vacuna actual contra el virus de la fiebre aftosa. A los 28 días posteriores a la vacunación, se recolectaron muestras de suero y se analizaron contra el virus de la fiebre aftosa SAT2 Libia adaptado a cultivo de tejidos y SAT2/EGY/2/2012 utilizando una prueba de neutralización viral para determinar la relación serológica (valor r1). El ensayo de reto en terneros vacunados se llevó a cabo empleando una cepa virulenta de la fiebre aftosa SAT2 Libia. Se encontró que los títulos de anticuerpos neutralizantes inducidos por los dos lotes de vacuna (1 y 2) y los de animales no vacunados, fueron 0,48, 0,39 y 0,15 log10 DICT50/mL, respectivamente, mientras que la prueba reveló valores de protección de 20 por ciento, 0 por ciento y 0 por ciento, respectivamente. Además, los valores de r1 fueron 0,195 y 0,186 para los lotes de vacuna (1 y 2), respectivamente. Se llegó a la conclusión de que los lotes de vacunas locales comerciales inactivadas disponibles actualmente (SAT2 SAT2/EGY/2/2012) no protegen a los terneros contra el virus circulante de la fiebre aftosa SAT2 topotipo VII, linaje Lib-12 que se aisló recientemente, por lo que es recomendable actualizar las vacunas existentes con la cepa aislada actualmente(AU)
Foot and mouth disease virus is a highly infectious and contagious pathogen. Recently the topotype VII, Lib‐12 lineage of serotype SAT2 was reported through outbreaks in Egypt during 2018. Vaccination is an effective way to control and combat the foot and mouth disease virus outbreaks especially in endemic areas like Egypt. The present study was aimed to evaluate the efficacy of the current produced foot and mouth disease vaccine, against the recently isolated field strain foot and mouth disease virus SAT2 topotype VII, Lib-12 lineage (SAT2 Libya), by applying in vitro and in vivo studies. Two batches of the current foot and mouth disease virus vaccine were inoculated in calves. At the 28th day post-vaccination serum samples were collected and tested against tissue culture adapted foot and mouth disease virus SAT2 Libya and SAT2/EGY/2/2012 using virus neutralization test to determine serological relationship (r1-value). The challenge test for vaccinated calves was carried out against the virulent foot and mouth disease virus SAT2 Libya. It was found that neutralizing antibody titers induced by the two vaccine batches (1 and 2) and those in unvaccinated animals were 0.48, 0.39 and 0.15 log10 TCID50/mL, respectively, while the challenge revealed protection values of 20 percent, 0 percent and 0 percent, respectively. Furthermore, the r1 values were 0.195 and 0.186 for vaccine batches (1 and 2), respectively. It was concluded that the available local commercial inactivated foot and mouth disease virus vaccine batches (SAT2 SAT2/EGY/2/2012) are unable to protect calves against the current circulating foot and mouth disease virus field isolate SAT2 topotype VII, Lib-12 lineage, thus it is highly recommended to update the existing vaccines with the present isolated strain(AU)
Asunto(s)
Animales , Ganado , Potencia de la Vacuna , Fiebre Aftosa/prevención & control , Fiebre Aftosa/epidemiologíaRESUMEN
Resumen La brucelosis es una zoonosis desatendida y un problema de salud pública en países en desarrollo, que también causa grandes pérdidas económicas en la ganadería. En México es endémica y Chiapas un estado al sur del país, destaca por la presencia de la enfermedad en humanos. Por medio de la revisión del estado del arte, se encontró que factores como la alta producción de ganado bovino, las limitadas estrategias institucionales de control de la enfermedad en el ganado, la falta de regulación sanitaria del género Brucella en productos lácteos y cárnicos y la ubicación del estado de Chiapas en la frontera con Centroamérica, podrían ser condicionantes para la prevalencia de brucelosis humana en esta región de México.
Abstract Brucellosis is a neglected zoonosis and a public health problem in developing countries that also causes great economic losses in livestock. In Mexico it is endemic and Chiapas, a state in the south of the country, stands out for the presence of the disease in humans. Through the review of the state of the art it was found that factors such as the high production of cattle, the limited institutional strategies to control the disease in livestock, the lack of sanitary regulation of the genus Brucella in dairy and meat products and the location of the state of Chiapas on the border with Central America could be determining factors for the prevalence of human brucellosis in this region of Mexico.
Asunto(s)
Humanos , Animales , Bovinos , Brucella , Brucelosis/veterinaria , Brucelosis/epidemiología , Zoonosis/epidemiología , Estudios Seroepidemiológicos , Prevalencia , Ganado , México/epidemiologíaRESUMEN
RESUMEN En muchos países la fasciolosis y la paramfistomosis representan un grave problema para la salud del sector pecuario. En Colombia hay registros de ambas distomatosis en bovinos, la mayoría en el trópico alto andino, las cuales generan pérdidas econômicas anuales cercanas a 40 000 000 000 COP. El objetivo de esta investigación fue determinar la prevalencia de fasciolosis y paramfistomosis en vacunos de la hacienda La Candelaria, Caucasia (Colombia), y la presencia de caracoles hospederos intermediarios. Para cumplirlo, se realizó un estudio descriptivo de corte transversal con muestreo no probabilístico por conveniencia. Se recolectaron heces de los animales y se hizo el diagnóstico mediante la técnica modificada de Dennis. Se establecieron las prevalencias de los digeneos de acuerdo con el sexo, peso, edad y raza. Se recolectaron caracoles dulciacuícolas en la zona estudiada y se identificaron por morfologia. Se analizaron 466 muestras fecales de 178 bovinos, de las razas Cebú (Bos indicus), BON (blanco orejinegro) y del cruce entre ellas. Se diagnosticaron F. hepatica y Paramphistomidae con prevalencias del 2,2% y 30,9%, respectivamente. En el 1,1% de los vacunos se diagnosticó coinfección. Ambas trematodosis prevalecieron en las hembras (p = 0,03). Se identificaron moluscos dulciacuícolas Ampullariidae, Physidae y Planorbidae sin estadios larvarios de digeneos. Se concluyó que los bovinos de doble propósito de la hacienda La Candelaria están expuestos a F. hepatica y Paramphistomidae, y, probablemente, se infectan en los predios de la hacienda. Paramphistomidae es más prevalente que F. hepatica, lo cual concuerda con lo descrito en algunos estudios realizados en hatos del trópico alto andino colombiano. Paramphistomidae se encontró en todos los grupos etarios.
ABSTRACT Fasciolosis and paramphistomosis are a major health problem for the livestock economy worldwide. In Colombia, both distomatosis are reported in cattle, particularly in high Andean tropics, with annual economic losses close to COP 40 billion. The goal of this study was to determine the prevalence of fasciolosis and paramphistomosis in cattle from La Candelaria farm, Caucasia (Colombia), and the presence of intermediate host snails. A descriptive cross-sectional study was carried out with non-probability convenience sampling. Stool samples were collected from the animals and the diagnosis was made using the modified Dennis technique. Digenea prevalence were determined according to sex, weight, age, and race. Freshwater snails were collected in the studied area and were identified by morphology. 400 and 66 fecal samples from 178 bovines of the Zebu (Bos indicus), BON (white-eared white) breeds and the cross between them were analyzed. Fasciola hepatica and Paramphistomidae were diagnosed with a prevalence of 2,2% and 30,9%, respectively. Coinfection was diagnosed in 1,1% of the cattle. Both trematodosis were most frequent in females (p = 0,03). Freshwater molluscs Ampullariidae, Physidae and Planorbidae without digenea larval stages were identified. It was concluded that dual-purpose cattle from La Candelaria farm are exposed to F. hepatica and Paramphistomidae and are probably infected on the farm grounds. Paramphistomidae was more prevalent than F. hepatica, which agrees with other studies in herds from the Colombian high Andean tropics. Paramphistomidae was found in all age groups.
Asunto(s)
Animales , Bovinos , Bovinos , Ecosistema Tropical , Fasciola hepatica , Ganado , Coinfección , Agua Dulce , Moluscos , Investigación , Caracoles , Registros , Estudios Transversales , DiagnósticoRESUMEN
This study aims to determine the prevalence of Fasciola hepatica in cattle from the Abancay, Curahuasi, and Tamburco districts in the Abancay province, Apurímac, Peru, during the 2018 rainy season, and the association between prevalence and age, breed, and district of origin. In total, 295 stool samples were collected, namely 34 from Tamburco, 193 from Curahuasi and 68 from Abancay. For coproparasitological evaluation, the four-sieve sedimentation technique described by Girão and Ueno was used. The total prevalence of F. hepatica in the cattle sampled in this study was 50.8% (150/295), and the prevalence by district was 42.6% (29/68) in Abancay, 53.8% (104/193) in Curahuasi, and 50% (17/34) in Tamburco. No significant association was found with the variable district of origin (p<0.05). However, using a bivariate logistic regression analysis, a significant association was found between F. hepatica prevalence and the breed variable (p=0.008). A similar significant association with the breed variable (p=0.007) was also found using a multiple logistic regression analysis. The high prevalence of F. hepatica identified in this study is consistent with previous reports made in the Apurímac Region, an area considered hyperendemic for the parasite, thus highlighting the need for effective health programs to control disease distribution, which may have an economic and, because of its zoonotic character, public health impact.
Asunto(s)
Parásitos , Fasciola hepatica , Ganado , HepatopatíasRESUMEN
The use of blood metabolites (BM), fecal starch (FS), and apparent digestion of starch, (ATTSD) as indicators of feed efficiency (FE) in beef cattle in the feedlot was studied. Fourteen bulls were used, originating in an industrial cross, without a defined racial group, with mean body weight of 284.86kg, individually fed, being evaluated in a 42-day confinement system. After the evaluation, the animals were divided into two groups according to the individual FE: high feed efficiency (HE) and low feed efficiency (LE). There was a difference between the groups in the variables FE, feed conversion (FC), final weight (FW), and daily weight gain (DWG). The FE had a positive correlation with DWG, FC, and FW. There was no difference between the groups for the variables BM, FS, and ATTSD, nor was there any correlation between these variables and FE. Considering the feed cost, the HE animals proved more profitable. BM, FS, and ATTSD did not statistically show potential to be used as indicators of FE, despite the evidence of numerical differences of these variables between the different groups, tendency of correlations with FE, and discriminating function with potential assertiveness.(AU)
Foi estudada a utilização dos metabólitos sanguíneos (BM), do amido fecal (FS) e da digestão aparente do amido (ATTSD) como indicadores de eficiência alimentar (FE) em bovinos de corte em confinamento. Utilizaram-se 14 touros, originários de cruzamento industrial, sem grupo racial definido, peso corporal médio de 284,86kg, alimentados individualmente, sendo avaliados em sistema de confinamento por 42 dias. Após a avaliação, dividiram-se os animais em dois grupos, de acordo com a FE individual: alta eficiência alimentar (HE) e baixa eficiência alimentar (LE). Houve diferença entre os grupos nas variáveis FE, conversão alimentar (FC), peso final (FW) e ganho de peso diário (DWG). A FE teve correlação positiva com DWG, FC e FW. Não houve diferença entre os grupos para as variáveis BM, FS e ATTSD, tampouco houve correlação entre essas variáveis e a FE. Considerando-se o custo alimentar, os animais HE mostraram-se mais lucrativos. BM, FS e ATTSD não mostraram, estatisticamente, potencial para serem utilizados como indicadores de FE, apesar da evidência de diferenças numéricas dessas variáveis entre os diferentes grupos, tendência de correlações com a FE e de função discriminante com potencial assertividade.(AU)
Asunto(s)
Animales , Bovinos , Aumento de Peso , Ganado/sangre , Fenómenos Fisiológicos Nutricionales de los Animales , Peso Corporal , Costos y Análisis de CostoRESUMEN
ABSTRACT Serological controls for diseases of viral origin in animal production systems and the identification of factors associated with infections are decisive elements to establish prevention and control measures. The aim of this study was to establish the serological status for Bovine Viral Diarrhea (BVD) and Enzootic Bovine Leukemia (EBL) viruses in Blanco Orejinegro (BON) cattle from Colombia, and to identify the factors associated with seropositivity. A cross-sectional study was conducted with a total of 498 animals of all age groups and physiological states of the BON breed were selected, belonging to 14 herds located in 6 states of Colombia, in which a survey with 27 questions was conducted. By means of the chi-square test, possible factors associated with seropositivity against the 2 viruses were identified. A sample of 4 ml of blood was taken from each animal to extract plasma and make indirect Elisa tests to detect antibodies against both pathogens. General seropositivity of 27,1% was obtained for EBL, finding as factors associated with seropositivity the inadequate disposition of placental tissues after delivery of the cows and the non-performance of serological tests on new animals entering the herd. For BVD, seropositivity obtained was 50,6%, and the factors associated with seropositivity identified were having had a history of the disease in the herd, and using semen from bulls that are not known to be free for the infection. We suggest establishing control measures considering the factors associated with each viral infection to limit their expansion in the BON cattle production systems of Colombia.
RESUMO Os controles sorológicos para doenças de origem viral nos sistemas de produção animal e a identificação de fatores associados à infecção são elementos decisivos para estabelecer medidas de prevenção e controle. O objetivo deste estudo foi estabelecer o status sorológico dos vírus da Diarréia Viral Bovina (BVD) e da Leucemia Bovina Enzoótica (EBL) em bovinos Blanco Orejinegro (BON) da Colômbia e identificar os fatores associados à soropositividade. Foram selecionados 498 animais de todas as faixas etárias e estados fisiológicos da raça BON, de 14 rebanhos localizados em seis regiões da Colômbia, nos quais foi realizada uma pesquisa para identificar possíveis fatores associados à soropositividade contra os dois vírus. Foi retirada uma amostra de 4 ml de sangue de cada animal para extrair plasma e fazer testes ELISA indiretos para detectar anticorpos contra os dois patógenos. Obteve-se soropositividade geral de 27,1% para EBL, encontrando como fatores associados à soropositividade a disposição inadequada dos tecidos placentários após o parto das vacas e a não realização de testes sorológicos em novos animais que entraram no rebanho. Para a BVD, a soropositividade obtida foi de 50,6%, e os fatores associados à soropositividade identificados foram: histórico de doença no rebanho e uso de sêmen de touros que não são reconhecidos como livres da infecção. Sugerimos o estabelecimento de medidas de controle considerando os fatores associados a cada infecção viral para limitar sua expansão nos sistemas de produção de gado BON da Colômbia.
Asunto(s)
Virosis , Bovinos , Ensayo de Inmunoadsorción Enzimática , Pruebas Serológicas , Leucemia , Estudios Transversales , Leucosis Bovina Enzoótica , Virus de la Diarrea Viral Bovina , Infecciones , Semen , Diarrea , GanadoRESUMEN
This report aims to discuss the occurrence of protozoan infestation in a Girolanda cattle herd in the state of Bahia / BA. Bovine trypanosomiasis is a severe disease that is harmful to cattle, inducing large productive and economic losses. The animals are affected by protozoa of the genus Trypanosoma spp., giving rise to infestations in the vast majority of them in a silent and lasting way. In the Bahian rural property described, 10% of adult cows died and the rest of the animals showed clinical signs of anemia, drop in milk production, low body score, apathy, among others. Among the information obtained through the farm manager, who helped in the diagnosis and in ordering tests for the respective disease were: purchase of cattle from the region of the state of Minas Gerais and dubious management of oxytocin for improvements in milk production (suspected shared needles). Faced with the suspicion and in conjunction with the information obtained, laboratory tests and molecular tests were carried out to confirm the suspicion. After the examinations, it was found that the animals were affected by protozoa of the genus Trypanossoma vivax. The animals were treated with volume replenishers and drugs such as isometamidium chloride in a single dose calculated according to the body weight.
O presente relato tem por objetivo discorrer sobre a ocorrência da infestação protozoária em um rebanho bovino da raça Girolanda no estado da Bahia/BA. A tripanossomíase bovina é uma doença severa e prejudicial aos bovinos, induzindo-os a grandes perdas produtivas e econômicas. Os animais são afetados por protozoários do gênero Trypanosoma spp., dandoorigem a infestações em sua grande maioria de forma silenciosa e duradoura. Na propriedade rural baiana descrita, 10% das vacas adultas morreram e o restante dos animais apresentava sinais clínicos de anemia, queda da produção leiteira, baixo escore corpóreo, apatia, prostração, entre outros. Dentre as informações obtidas através do gerente da fazenda, que auxiliaram no diagnóstico e na requisição de exames para a respectiva doença estavam: compra de bovinos da região do estado de Minas Gerais e manejo duvidoso de ocitocina para melhorias na produção leiteira (suspeita de agulhas compartilhadas). Diante da suspeita e em conjunto com informações obtidas, foram realizados exames laboratoriais e testes moleculares para confirmar a suspeita. Após a realização dos exames, constatou-se que os animais estavam acometidos por protozoários do gênero Trypanossoma vivax. Os animais foram tratados com repositores de volume e fármacos como o cloreto de isometamidium em uma única dose calculada de acordo com o peso corpóreo.
Asunto(s)
Animales , Bovinos , Infecciones por Protozoos , Tripanosomiasis Bovina , Enfermedades de los Bovinos , Trypanosoma , GanadoRESUMEN
BACKGROUND: This study aimed to explore genetic polymorphisms of the CCKAR gene and their relationship with the growth and development of Qinchuan cattle which could be used as molecular markers for the improvement of the breeding of Qinchuan cattle. RESULTS: Here, we have identified seven single nucleotide polymorphisms (SNPs) at loci g. 1463 C>G; g. 1532 T>A; g. 1570 G>A; g. 1594 C>A; g. 1640 T>C; g. 1677 G>C; and g. 1735 C>T in the coding region of the bovine CCKAR gene. The frequencies identified on allelic and genotypic characteristics have shown that all seven SNPs diverged from the Hardy-Weinberg-Equilibrium. The SNP2, SNP3, SNP6 and SNP7 had the lowest polymorphism information content values, and remaining SNPs were found to be moderate (0.25 < PIC < 0.50). The genotype CG in SNP1 at loci g.1463 C>G had the greatest association with WH, HW, CD and CCF, while the genotype TA at the very same loci was associated with BFT, ULA and IMF content in Qinchuan cattle. The CCKAR gene expression level in adipose tissue, small intestine, liver and skeleton muscle was found to be higher, whereas, the expression level of mRNA in organs of other digestive system including reticulum, abomasum and omasum was moderate. Some expression of CCKAR mRNA was found in the large intestine, kidney and rumen. CONCLUSIONS: In summary, our finding suggested that the CCKAR gene could be used as a potential candidate for the improvement of carcass quality and body measurements of Qinchuan cattle.
Asunto(s)
Animales , Bovinos , Bovinos/genética , Receptor de Colecistoquinina A/genética , Variación Genética , Desequilibrio de Ligamiento , Reacción en Cadena de la Polimerasa/métodos , Polimorfismo de Nucleótido Simple , Sistema Digestivo , Ganado , Técnicas de Genotipaje , Frecuencia de los Genes , Productos de la CarneRESUMEN
BACKGROUND: Transmembrane protein 95 (TMEM95) plays a role in male fertility. Previous studies showed that genes with a significant impact on reproductive traits can also affect the growth traits of livestock. Thus, we speculated that the genetic variation of TMEM95 gene may have effects on growth traits of cattle. RESULTS: Two SNPs were genotyped. The rs136174626 and rs41904693 were in the intron 4 and 30 -untranslated region, respectively. The linkage disequilibrium analysis illustrated that these two loci were not linked. The rs136174626 was associated with six growth traits of Nanyang cattle, four traits of Luxi cattle, and three traits of Ji'an cattle. For rs41904693 locus, the GG individuals had greater body height and abdominal girth in Ji' an cattle than TT and TG individuals. In Jinnan cattle, GG and TT individuals had greater body height, height at hip cross, body length, and heart girth than TG individuals. The potential splice site prediction results suggest that the rs136174626 may influence the splicing efficiency of TMEM95, and the miRNA binding site prediction results showed that the rs41904693 may influence the expression of TMEM95 by affecting the binding efficiency of Bta-miR-1584 and TMEM95 30 -UTR. CONCLUSIONS: The findings of the study suggested that the two SNPs in TMEM95 could be a reliable basis for molecular breeding in cattle.
Asunto(s)
Animales , Bovinos , Bovinos/genética , Polimorfismo de Nucleótido Simple , Proteínas de la Membrana/genética , Variación Genética , Bovinos/crecimiento & desarrollo , Barajamiento de ADN , Ganado , Técnicas de Genotipaje , Frecuencia de los GenesRESUMEN
Resumen Objetivo: Aislar STEC en las heces del ganado bovino en el municipio de Ulloa, Valle del Cauca y detectar factores de virulencia asociados con la patogénesis. Materiales y métodos: Se tomaron 21 muestras provenientes de bovinos, las cuales fueron tomadas directamente del recto del animal mediante hisopos. Las muestras se procesaron hasta obtener colonias puras a las cuales se les evaluó la presencia de los genes stx1, stx2, eae, saa y hlyA mediante PCR y posteriormente, se evaluó el efecto citotóxico de las muestras positivas sobre células Vero (ATCC-CCL-81.4). Resultados: De las 21 muestras de heces de bovinos,12 presentaron bacterias con uno o ambos genes stx. Se obtuvieron 106 aislamientos totales de STEC y se observaron diferencias en cuanto a la presencia y ausencia de los genes de virulencia evaluados en los aislamientos de cada bovino, obteniendo cinco combinaciones de genes. 48 aislamientos presentaron únicamente el gen stx2 y 58 presentaron tanto el gen stx1 como el gen stx2; de los 106 aislamientos, se detectaron 44 con el gen hlyA y 57 con el gen saa. Conclusiones: Todos los sobrenadantes de STEC analizados mostraron actividad citotóxica sobre las células Vero, mientras que en ausencia de STEC las células formaron monocapa después de 48 h de incubación. Este trabajo es el primer reporte en Colombia que aporta información sobre la presencia de STEC en el ganado bovino, la presencia de factores de virulencia y el potencial efecto citotóxico que poseen estas cepas nativas.
Abstract Objective: To isolate STEC in stool samples from cattle in Ulloa, Valle del Cauca, and to detect virulence factors associated with its pathogenesis. Materials and methods: We took 21 samples from cattle, which were taken directly from the rectum of the animal using swabs. The samples were processed until obtaining pure colonies and evaluated for the presence of the stx1, stx2, eae, saa and hlyA genes by PCR. Afterward, the cytotoxic effect of positive samples were evaluated on Vero cells (ATCC-CCL- 81.4). Results: We observed that from the 21 stools samples, 12 presented bacteria with one or both stx genes. A total of 106 isolates of STEC were obtained and differences among each other were observed regarding the presence and absence of the virulence genes, obtaining five combinations of genes. We found that 48 isolates presented only stx2 gene and 58 presented both the stx1 and stx2 gene. Regarding the other virulence genes, the hlyA gene was detected in 44 isolates and the saa gene was detected in 57 isolates. Conclusions: All the STEC supernatants showed cytotoxic activity on Vero cells, while in its absence the cells formed monolayer after 48 h of incubation. This work is the first report in Colombia that provides information about the presence of STEC in stool cattle, virulence genes and its potential cytotoxic effect in native strains.
Asunto(s)
Animales , Bovinos , Toxina Shiga , Escherichia coli , Escherichia coli Shiga-Toxigénica , Heces , Ganado , Bacterias , Virulencia , Reacción en Cadena de la PolimerasaRESUMEN
The reproductive efficiency of livestock is the basis for the success of livestock, dairy or beef, and having high reproductive performance depends on several factors within the production system and the presence of infectious diseases of the reproductive sphere in the herd is one of the factors that can compromise that efficiency. The aim of this study was to use molecular biology as a diagnostic tool for the detection of Leptospira spp. DNA in cows with reproductive disorders on a rural property in the municipality of Boca do Acre, Amazonas, Brazil. Vaginal mucus was collected from nine Nelore breeding cows with a history of abortion and birth of weak calves submitted to DNA extraction and nested-PCR technique for 16S gene amplification at the bacterial genus level. Of the nine samples analyzed, five (55.55%) amplified a product of 331bp. The municipality of Boca do Acre is bordered by Peru and Bolivia, and knowledge of the prevalence of the disease, serovars, and circulating Leptospira species is essential for the adoption of measures related to animal husbandry, as well as health education for ranchers and their workers to avoid a possible occupational infection since this disease is considered an important zoonosis. New molecular studies using primers that allow the identification of the Leptospira species and mainly pathogenic species should be conducted in this region in order to elucidate the possible species of this etiological agent and the possible reservoirs of the disease to begin the understanding of the epidemiology of this disease in cattle in this region of border.
Asunto(s)
Reproducción , Ganado , Leptospirosis/diagnósticoRESUMEN
Diseases of the bovine digestive system make up an important group of diseases, often being responsible for significant economic losses in the livestock sector. The current work aimed to carry out a retrospective study of the diseases of the digestive system in cattle diagnosed at the "Clínica de Bovinos de Garanhuns", "Universidade Federal Rural de Pernambuco", from January 1999 to December 2018. In this period, a total of 9,343 cattle were admitted to the CBG-UFRPE, of which 2,238 (24.0%) were diagnosed with diseases of the digestive system. In two decades, the total number of cattle admitted annually by the institution almost doubled as well as the number of cases of digestive diseases. The diseases categorized as mechanical/motor were the most prevalent (33.6%), followed by fermentative (28.4%), gastroenteritis (16.0%), esophageal diseases (9.7%), and diseases of the oral cavity (4.5%). Lesions of the rectum and anus, congenital alterations, and other digestive diseases showed relative frequencies below 4%. Traumatic reticulitis and its sequelae (14.5%) are the most prevalent disorders, followed by simple indigestion (10.1%), esophageal and intestinal obstructive disorders (9.0%), non-specific gastroenteritis (8.6%), displaced abomasum (RDA and LDA) (5.5%), and frothy bloat, ruminal lactic acidosis, and impaction of the forestomach and abomasum, which represented approximately 5% each. In general, these diseases presented a lethality rate of 46.0%, with emphasis on mechanical/motor diseases with a lethality rate of 73.8%. In general, diseases were more prevalent in females, crossbreeds, aged over 24 months, and raised in a semi-intensive system. Diseases of the digestive system increased over the years studied, representing a considerable portion of the diseases that affect cattle raised in this region, which makes up the main milk basin in the State of Pernambuco, confirming its economic and social impact in the region.(AU)
As enfermidades do sistema digestório dos bovinos compõem importante grupo de doenças frequentemente responsáveis por perdas econômicas significativas no setor pecuário. Este trabalho teve por objetivo realizar um estudo retrospectivo das enfermidades do sistema digestório dos bovinos diagnosticadas na Clínica de Bovinos de Garanhuns, Universidade Federal Rural de Pernambuco, no período de janeiro de 1999 a dezembro de 2018. Neste período foi atendido um total de 9.343 bovinos dos quais 2.238 (24,0%) foram diagnosticados com enfermidades do sistema digestório. Em duas décadas, o número total de bovinos atendidos anualmente pela instituição quase dobrou, assim como o número de casos de doenças digestivas. As enfermidades categorizadas como de natureza mecânica/motora foram as mais prevalentes (33,6%), seguidas das fermentativas (28,4%), das gastroenterites (16,0%), das enfermidades esofágicas (9,7%) e das enfermidades da cavidade oral (4,5%). As lesões do reto e ânus, as alterações congênitas e outras enfermidades digestórias apresentaram frequências relativas inferiores a 4%. As reticulites traumáticas e suas sequelas (14,5%) destacaram-se como as doenças mais prevalentes, seguidas das indigestões simples (10,1%), dos transtornos obstrutivos esofágicos e intestinais (9,0%), das gastroenterites inespecíficas (8,6%), dos deslocamentos de abomaso (DAD e DAE) (5,5%) e do timpanismo espumoso, acidose láctica ruminal e compactações dos pré-estômagos e abomaso, que representaram aproximadamente 5% cada. No geral estas enfermidades apresentaram taxa de letalidade de 46,0%, com destaque para as enfermidades de natureza mecânica/motora cuja letalidade foi de 73,8%. De maneira geral as enfermidades foram mais prevalentes em fêmeas mestiças, com idade superior a 24 meses, criadas em sistema semi-intensivo. As enfermidades do sistema digestório, crescentes ao longo dos anos estudados, representaram parcela considerável das doenças que acometem os bovinos criados na região que compõe a principal bacia leiteira do Estado de Pernambuco, ratificando seu impacto econômico e social para a região.(AU)
Asunto(s)
Animales , Bovinos , Estaciones del Año , Estudios Retrospectivos , Sistema Digestivo , Ganado , Gastroenteritis , UniversidadesRESUMEN
At present, there is a concern about the quality of milk and diseases related to its consumption, as it can generate discomfort and allergic reactions in some individuals due to its protein components. Thus, the present study was developed to identify the allele and genotype frequencies of genes for ß-casein, A1 and A2, in dairy herds in the region of Araguaína-TO, Brazil. Genetic material from 421 animals (crossbred dairy cattle in lactation) was used. All animals were numbered for identification, and DNA samples were extracted from hair bulbs. Samples for two markers from the polymorphic regions were characterized and confirmed by real-time PCR using the ABI Prism® 7500 Sequence Detection System (Applied Biosystems). Allele and genotype frequencies were determined using the TaqMan™ detection system, where the primer and probe release different fluorescence signals for each allele of the polymorphism. The sampled herd showed frequencies of 28.27% for the A1 allele and 71.73% for the A2 allele. Genotype frequencies were 52.96% (223/421) for A2A2; 37.53% (158/421) for the A1A2 genotype; and 9.50% (40/421) for the A1A1 genotype. The frequency of the A1 allele for ß-casein in dairy herds from the northern region of Tocantins was low and is per the results of previous studies. Although the A2A2 genotype of ß-casein had a high relative frequency, the A1A2 genotype is still rather frequent, warranting greater selection pressure.(AU)
Atualmente existe uma preocupação em relação à qualidade e doenças que estão relacionadas ao consumo de leite, pois o mesmo pode gerar desconfortos e reações alergicas em alguns indivíduos devido aos seus constituintes protéicos. Assim, o presente estudo teve como objetivo identificar a frequência alélica e genotípica de genes para beta caseína, A1 e A2, em rebanhos leiteiros da região de Araguaína-TO. Foram utilizados material genético de 421 animais (bovinos leiteiros mestiços em lactação), e todos os animais foram numerados para identificação e amostras de DNA foram extraídas de bulbo de folículos pilosos. As amostras para dois marcadores das regiões polimórficas foram caracterizadas e confirmadas por PCR em tempo real, usando um sistema de detecção de sequências ABI Prism® 7500 (Applied Biosystems). As frequências alélicas e genotípicas foram determinadas utilizando o sistema de detecção TaqMan ™, no qual o primer e a sonda emitem diferentes sinais de fluorescência para cada alelo do polimorfismo. Observou-se frequência do alelo A1 de 28,27%, e do alelo A2 de 71,73% no rebanho amostral. A frequência genotípica de A2A2 foi de 52,96% (223/421), com genótipo A1A2 de 37,53% (158/421), e de 9,50% (40/421) animais com genótipo A1A1. A frequência do alelo A1 para beta-caseína em rebanhos leiteiros da região norte do Tocantins foi baixa e seguiu a mesma tendência já observada em estudos anteriores. Os genótipos A2A2 da beta-caseína apresentaram frequência relativa alta, entretanto o genótipo A1A2 ainda é bastante frequente, necessitando de maior pressão de seleção.(AU)
Asunto(s)
Animales , Bovinos , Caseínas/administración & dosificación , Leche/química , Alelos , Ganado/genética , Genotipo , Reacción en Cadena de la PolimerasaRESUMEN
The application of spatial and temporal analysis in epidemiology aims to understand the causes and consequences of spatial and temporal heterogeneity in studies of infectious diseases. Bovine tuberculosis (bTB) is a chronic and progressive infectious disease caused by Mycobacterium bovis and is an important zoonosis worldwide. The aim of this study was to conduct spatial and temporal analyses of a secondary database of bTB-positive cases registered by the Federal Inspection Service in two slaughterhouses from the West of Minas mesoregion of Minas Gerais state, Brazil, from 2008 to 2012 and to suggest its use in epidemiological surveillance. The culled cattle with bTB macroscopic lesions during post mortem inspection were considered positive in this study. The data used were the positive cases registered on the "Sistema de Informações Gerenciais do Serviço de Inspeção Federal" (SIG/SIF - Management Information System of the Federal Inspection Service) of the "Ministério da Agricultura, Pecuária e Abastecimento" (MAPA - Ministry of Agriculture, Livestock and Supplies), the number of animals slaughtered monthly and their municipalities of origin, per slaughterhouse. The prevalence (%) of cases per cattle slaughtered was calculated, and the relationship between these cases of bTB and cases of human tuberculosis (hTB) and the GDP per capita of the mesoregions comprising the municipalities surveyed was evaluated. The prevalence was 1,030 cases of bTB (0.28%). The lowest case numbers and prevalence rates were obtained in 2010, while the highest rates were observed in 2011. Slaughterhouse 1 showed stationarity, while Slaughterhouse 2 showed an increasing annual trend for data since October 2009. A statistical correlation between the numbers of cases of bovine and human TB (p=0.006, r=0.148) was observed. The prevalence for Slaughterhouses 1 and 2 were not correlated (p>0.05). The municipalities of origin demonstrated randomness in their spatial distribution. SIG/SIF-MAPA may be used for the analysis of spatial and temporal distribution to contribute to the monitoring of animal health services with information on the prevalence of culling for bTB in the West of Minas, South/Southwest of Minas, Campo das Vertentes and Zona da Mata mesoregions of Minas Gerais, Brazil.(AU)
A aplicação de análises espacial e temporal em epidemiologia visa compreender as causas e consequências da heterogeneidade espacial e temporal nos estudos de doenças infecciosas. A tuberculose bovina (TBb) é uma doença infecciosa crônica e progressiva causada pelo Mycobacterium bovis e uma importante zoonose em todo o mundo. O objetivo deste estudo foi realizar análises espaciais e temporais em um banco de dados secundário de casos positivos de TBb registrados pelo Serviço de Inspeção Federal em dois frigoríficos da mesorregião Oeste de Minas, estado de Minas Gerais, Brasil, de 2008 a 2012 e sugerir sua utilização na vigilância epidemiológica. Os bovinos abatidos com lesões macroscópicas de TBb durante a inspeção post mortem foram considerados positivos neste estudo. Os dados utilizados foram os casos positivos cadastrados no Sistema de Informações Gerenciais do Serviço de Inspeção Federal do Ministério da Agricultura, Pecuária e Abastecimento (SIGSIF/MAPA), o número de animais abatidos mensalmente e seus municípios de origem, por frigorífico. Foi calculada a prevalência (%) de casos por bovinos abatidos e avaliada a relação entre esses casos de TBb e os casos de tuberculose humana (TBh) e o PIB per capita das mesorregiões que compõem os municípios pesquisados. A prevalência foi de 1.030 casos de TBb (0,28%). Os menores números de casos e prevalências foram obtidos em 2010, enquanto as maiores prevalências foram observadas em 2011. O Frigorífico 1 apresentou estacionariedade, enquanto o Frigorífico 2 apresentou tendência anual crescente para os dados desde outubro de 2009. Correlação estatística entre o número de casos de TBb e TBh (p=0,006, r=0,148) foi observada. As prevalências nos Frigoríficos 1 e 2 não se correlacionaram (p>0,05). Os municípios de origem demonstraram aleatoriedade em sua distribuição espacial. O SIGSIF/MAPA pode ser utilizado para a análise da distribuição espacial e temporal a fim de contribuir com o monitoramento dos serviços de saúde animal com informações sobre a prevalência de condenações por TBb nas mesorregiões Oeste de Minas, Sul/Sudoeste de Minas, Campo das Vertentes e Zona da Mata de Minas Gerais, Brasil.(AU)
Asunto(s)
Animales , Bovinos , Tuberculosis Bovina/epidemiología , Distribución Temporal , Ganado , Monitoreo Epidemiológico , Análisis Espacial , Mycobacterium bovis/patogenicidadRESUMEN
Fipronil was registered in Uruguay in 1997, and, since then, it has been used for the control of Haematobia irritans irritans and Rhipicephalus microplus. The susceptibility of H. irritants to this drug has not been evaluated. Therefore, the goal of the present study was to evaluate the resistance of H. irritans to fipronil. Additionally, a survey was carried out with the farmers to evaluate the use of fipronil for H. irritans control in the ranches where the flies came from. For the bioassays, 31 field populations of H. irritans were exposed to 10 concentrations of fipronil (3.2-16.0μg.cm2), and their LC50 values were calculated using probit analysis. A bioassay was performed with horn flies from the susceptible colony maintained at the USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory for comparison and calculation of resistance ratios (RRs). All 31 field populations surveyed in the study were susceptible to fipronil, with resistance ratios ranging from <0.5 to 2.2. Four populations with RRs >1 did not differ significantly from the susceptible strain. A single population showed an RR >2.2. Overall, the survey shows that fipronil was mostly used for R. microplus control, and in only three ranches, which were free of R. microplus, was fipronil used for horn fly control. Seventeen farmers did not use fipronil at all in the last three years. It is concluded that, in Uruguay, field populations of horn flies remain susceptible to fipronil.(AU)
O fipronil foi registrado no Uruguai em 1997 e, desde então, tem sido utilizado no controle de Haematobia irritans irritans e Rhipicephalus microplus. O objetivo do presente estudo foi avaliar a susceptibilidade de populações de campo de H. irritans ao fipronil. Além disso, foi realizada uma pesquisa para avaliar a utilização de fipronil e as práticas de controle de H. irritans nas fazendas de onde provinham as moscas. Para os bioensaios, 31 populações de campo de H. irritans foram expostas a 10 concentrações de fipronil (3,2-16,0μg.cm2), e seus valores de CL50 foram calculados usando análise probit. Um bioensaio foi realizado com H. irritans da colônia suscetível mantida no USDA-ARS Knipling-Bushland U.S. Livestock Insects Research Laboratory para comparação e cálculo das razões de resistência (RRs). Todas as 31 populações de campo pesquisadas no estudo eram suscetíveis ao fipronil, com taxas de resistência variando de <0,5 à 2,2. Quatro populações com Rrs >1 não diferiram significativamente da cepa suscetível. Uma única população apresentou RR >2,2. No geral, o fipronil tinha sido usado principalmente para o controle de R. microplus, e em apenas três fazendas, que estavam livres de R. microplus, o fipronil era utilizado para o controle da H. irritans. Em 17 fazendas não tinha sido utilizado fipronil nos últimos três anos. Conclui-se que no Uruguai as populações de H. irritans no campo permanecem suscetíveis ao fipronil.(AU)
Asunto(s)
Animales , Bovinos , Bioensayo , Control Biológico de Vectores , Rhipicephalus/patogenicidad , Dípteros , Ganado , Encuestas y Cuestionarios , Susceptibilidad a Enfermedades , LaboratoriosRESUMEN
This study aimed to establish the prevalence of animals persistently infected (PI) with bovine viral diarrhea virus (BVDV) in dairy farms at Parana State, Brazil. Samples were collected from 6,465 female Holstein Friesian Dairy Cattle, including animals less than two years old, females over two years old who had not given birth at the farm, and mothers of calves diagnosed as persistently infected. The cattle came from 40 dairy herds distributed in 10 municipalities in the State of Paraná. The samples were obtained from May 2015 to August 2018. The diagnosis of PI animals was made with an antigen-capture ELISA test. We detected PI animals in fifteen herds sampled (37.5%), ranging from one to sixteen animals per herd. The prevalence in Parana State's municipalities was 1.78%, ranging from 0.3 to 8.9% at positive herds. The analysis of the individual herds shows significant dissemination of the BVDV in Parana's municipalities, including endemic areas. With this, we highlight the need for measures to raise awareness among producers about the existence and importance of bovine viral diarrhea (BVD) in dairy herds, reinforcing the PI animals' role in disease epidemiology and the economic impact caused by the maintenance of these farm animals.(AU)
Com o intuito de se estabelecer a prevalência de animais persistentemente infectados (PI) com o BVDV em propriedades leiteiras no estado do Paraná. Foram coletadas amostras de 6.465 bovinos, fêmeas, da raça Holandês Preto e Branco (HPB). Amostraram-se animais com idade inferior a dois anos, fêmeas com mais de dois anos que não haviam tido partos na propriedade, e mães de bezerros que foram diagnosticados como persistentemente infectados. Os bovinos foram provenientes de 40 rebanhos leiteiros, distribuídos em 10 municípios no Estado do Paraná. A coleta deu-se no período de maio de 2015 a agosto de 2018. O diagnóstico dos animais PI foi feito por meio do teste de ELISA de captura de antígeno. Animais PI foram detectados em quinze rebanhos amostrais (37,5%), oscilando entre um e dezesseis animais por rebanho. A prevalência nos municípios do estado Paraná foi de 1,78%, oscilando entre 0,3 a 8,9% nos rebanhos positivos. Com a alta prevalência de animais PI observada, quando analisados os rebanhos amostrais individualmente, é possível afirmar que há uma disseminação importante do BVDV em municípios paranaenses, destacando inclusive áreas endêmicas. Com isso, vê-se a necessidade de medidas de conscientização dos produtores sobre a existência e importância da BVD nos rebanhos, destacando o papel dos animais PI na epidemiologia da doença, bem como o impacto econômico causado pela manutenção desses animais nos rebanhos.(AU)
Asunto(s)
Animales , Bovinos , Prevalencia , Virus de la Diarrea Viral Bovina , Ganado , Animales Domésticos , DiarreaRESUMEN
Resumen La brucelosis, principal zoonosis a nivel mundial tiene alta prevalencia en varios países de Latinoamérica. Se asocia con la exposición a ganado infectado por distintas especies del género Brucella. B. melitensis la más virulenta para el humano, causa con frecuencia complicaciones de predominio osteoarticular. En Colombia se cree que la infección por B. melitensis es una entidad ausente, a pesar de su plausibilidad biológica en nuestro contexto; sin embargo, son escasos los estudios sobre su ocurrencia y mínimo el índice de sospecha de la enfermedad, por lo cual creemos está subdiagnosticada. Presentamos el primer caso confirmado de brucelosis por B. melitensis en Colombia en una joven embarazada, con diagnóstico incidental, en quien el análisis retrospectivo de su cuadro clínico alertó sobre puntos clave que pueden impactar en el diagnóstico y tratamiento oportuno de la enfermedad. Se plantean preguntas de prevalencia real de esta entidad en Colombia.
Summary Brucellosis, the principal zoonoses globally is highly prevalent in different countries of Latin America. It is associated with the exposition of livestock infected with different Brucella species, being B. melitensis the most virulent for humans, and frequently causing osteoarticular complications. In Colombia it is believed that B. melitensis infection is an absent entity, despite its biological plausibility in our context; however, there are few studies on its occurrence and a minimum index of suspicion of the disease, which is why we believe it is underdiagnosed. We present the first confirmed case of brucellosis by B. melitensis in Colombia diagnosed in a young pregnant patient, with an incidental diagnosis, in whom a retrospective analysis of her clinical outcome warned of key points that may impact on the diagnosis and timely treatment of the disease. We present several questions surrounding the real prevalence of this entity in Colombia.