Total glycosides of Ranunculus japonius prevent hypertrophy in cardiomyocytes via alleviating chronic Ca(2+) overload / 中国医学科学杂志(英文版)

<p><b>OBJECTIVE</b>To evaluate the in vitro anti-hypertrophic effect of total Glycosides of Ranunculus Japonius (TGRJ).</p><p><b>METHODS</b>Neonatal rat cardiomyocytes were cultured and hypertrophy was induced by administrating isoproterenol (ISO, 10 µmol/L) or angiotensin 2 (Ang 2, 1 µmol/L) for 48 hours. In the treatment groups, cells were pretreated with TGRJ (0.3 g/L) for 30 minutes prior to hypertrophic stimuli. The anti-hypertrophic effects of TGRJ were examined by measuring cell size, total protein content, and protein synthesis. Intracellular free Ca(2+) concentration ([Ca(2+)]i) was evaluated using fluorescence dye Fura-2/AM. Sacroplasmic/endoplasmic reticulum Ca(2+) ATPase 2a (SERCA2a), atrial natriuretic peptide (ANP), B-type natriuretic peptide (BNP), and beta-myosin heavy chain (β-MHC) protein expression levels were measured by Western blotting . SERCA2a activity was assayed by p-nitrophenal phosphate disodium salt hexahydrate method.</p><p><b>RESULTS</b>Increased cell size, total protein content, and protein synthesis following ISO or Ang 2 stimulation were significantly inhibited by pretreatment with TGRJ (all P<0.05). This anti-hypertrophic effect of TGRJ was confirmed by its suppressing effect on elevated expression of the three hypertrophic related genetic markers, ANP, BNP, and β-MHC. In addition, TGRJ inhibited ISO or Ang 2 induced up-regulation of [Ca(2+)]i under chronic but not acute conditions. And ISO or Ang 2 induced down-regulation of SERCA2a expression and activity was also effectively rectified by TGRJ pretreatment.</p><p><b>CONCLUSIONS</b>The results of present study suggested that TGRJ could prevent ISO or Ang 2 induced cardiac hypertrophy through improving chronic [Ca(2+)]i disorder, might via normalizing SERCA2a expression and activity.</p>

Effects of polysaccharide of radix ranunculi ternati on immunomodulation and anti-oxidation / 中国中药杂志

<p><b>OBJECTIVE</b>To study effects of polysaccharide of Radix Ranunculi Ternati (PRT) on immunological function and anti-oxidation activity of mouse.</p><p><b>METHOD</b>Cell proliferations of splenocyte, thymocyte and peritoneal macrophage were measured by MTT colorimetry. The phagocytic function of peritoneal macrophage was measured by neutral red colorimetric method. The disoxidation power of PRT was measured by Prussian blue method. The clearing effect of PRT on hydroxyl radical was measured by salicylic acid capture method. The clearing effect of PRT on superoxide anion free radical was measured by pyrogallol auto oxidation method.</p><p><b>RESULT</b>PRT among 25-400 mg x L(-1) could enhance thymocytes and spleen lymphocyte proliferation and macrophage phagocytosis. PRT(200 mg x L(-1)) has the strongest macrophage proliferation. PRT in different concentration has shown some disoxidation effects. PRT in 8 g x L(-1) has nearly the same ability of clearing x OH by Vit C with the same concentration. The clearance rate of PRT on O2*- is 95.39%.</p><p><b>CONCLUSION</b>PRT can enhance the cell proliferation capability of thymocytes, spleen lymphocytes and peritoneal macrophages. PRT can enhance macrophage phagocytosis in a dose-response relationship. PRT has saome disoxidation power and strong ability of clearing x OH and O2*-.</p>

Studies on chemical constituents of Ranunculus japonicus / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and determine the chemical constituents of Ranunculus japonicus in Liupan Mountain, Ningxia province, China.</p><p><b>METHOD</b>The herb was extracted with ethanol by ultrasonic bath. The extractives were divided to petroleum ether, ethyl acetate, n-butanol parts. The first two parts were separated and purified with silica gel and Sephadex LH -20 column chromatography. The structures of the separated compounds were idnetefied by physical and chemical properties and spectral analysis.</p><p><b>RESULT</b>Nine compounds were isolated and identified as follows: scoparone (1), tricin (2), protocatechuic acid (3), luteolin (4), anemonin (5), scopoletin (6), 5-hydroxy-6, 7-dimethoxyflavone (7), ternatolide (8), 5-hydroxy-7, 8-dimethoxy-flavone (9).</p><p><b>CONCLUSION</b>Compounds 1-9 were isolated from Ranunculus japonicus for the first time.</p>

Studies on chemical constituents of Ranunculus ternatus / 中国中药杂志

<p><b>OBJECTIVE</b>To study the active constituents of Ranunculus ternatus.</p><p><b>METHOD</b>The constituents were isolated with silica gel and Sephadex LH -20 gel column chromatography and purified by HPLC. Their structures were elucidated by spectroscopy.</p><p><b>RESULT</b>Sixteen compounds were obtained and identified as Stigmasta4, 6, 8 (14), 22-tetraen-3-one (1), 5-hydroxymethyl furaldehyde (2), gamma-keto-delta-valerolactone (3), pantolactone (4), 5-hydroxymethyl-dihydro-furan-2-one (5), methyl 5-hydroxy-4-oxopentanoate (6), methyl hydrogen succinate (7), succinic acid monoethyl ester (8), 3, 4-dihydroxybenzoic acid methyl ester (9), p-hydroxycinnamic acid (10), 4-oxo-pentanoic acid (11), succinic acid (12), nonanedioic acid (13), 4-hydroxybenzoic acid (14), 4-hydroxybenzaldehyde (15), stigmasterol-3-O-beta-D-glucopyranoside (16).</p><p><b>CONCLUSION</b>Compound 1, 4 -15 are obtained from this plant for the first time.</p>

Studies on the chemical constituents in herb of Ranunculus japonicus / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and determine the chemical constituents from Ranunculus japonicus.</p><p><b>METHOD</b>Compounds were isolated by column chromatography, and identified by MS, NMR data.</p><p><b>RESULT</b>Nine compounds were obtained andelucidated as scoparone (1), tricin (2), protocatechuic acid (3), luteolin (4), anemonin (5), scopoletin (6), 5-hydroxy-6, 7-dimethoxyflavone (7), 5-hydroxy-7, 8-dimethoxyflavone (8), ternatolide (9), respectively.</p><p><b>CONCLUSION</b>Compounds 1-9 were isolated from R. japonicus for the first time while compounds 7, 8 were separated from Ranunculus genus first time.</p>

A Case of Irritant Dermatitis due to Ranunculus japonicus / 대한피부과학회지

Irritant dermatitis is the most frequent type of plant-related dermatitis. Plant families most commonly associated with irritation are Ranunculaceae, Euphorbiaceae, Cruciferae, Brassicaceae, Urticaceae, and Solanaceae. We report a case of irritant contact dermatitis caused by Ranunculus japonicus for the treatment of arthralgia in a 78 year old female.

Studies on the chemical constituents in herb of Ranunculus sceleratus / 中国中药杂志

<p><b>OBJECTIVE</b>To isolate and determine the chemical constituents from Ranunculus sceleratus.</p><p><b>METHOD</b>Six compounds were isolated and purified by extraction and different kinds of column chromatography. The structures were determined on the basis of extensive spectral analysis such as EI-MS, 1H-NMR, 13C-NMR, DEPT and NOEDS.</p><p><b>RESULT</b>The structures were elucidated as stigmasta-4-ene-3,6-dione I, stigmasterol II, isoscopoletin III, scoparone IV, protocatechuic aldehyde V and protocatechuic acid VI, respectively.</p><p><b>CONCLUSION</b>Compound I, III, IV, V and VI were isolated from R. genus for the first time.</p>

A Case of Contact Dermatitis due to Ranunculus sceleratus Linne / 대한피부과학회지

The Ranunculus sceleratus Linne is a plant which belongs to Ranunculus Linne, Ranu-nculaceae. We report a case of contact dermatitis due to Rannunculus sceleratus Linne. The patient developed painful, inflamed erythematous patches with a tense bullae on application sites of the crushed plant for the therapy of arthralgia on both knees. We did patch tests on a health volunteer and topical application of a rabbit's skin with its crushed leaf, stalk and root. Their results were compared with each other.

A Case of Primary Irritant Dermatitis due to Ranunculus Tachiorei / 대한피부과학회지

Ranunculus tachiorei is a member of the buttercup family (Ranunculaceae) which contains unsaturated lactone and protoanemonine. Protoanemonine is formed by the breakdown of the glycoside Ranunculin after injury to the plant and causes severe vesiculation and linear streaks after contact with field buttercups(Ranunculus spp.) The amount of protoanemonine in buttercups varies widely with the species of plant and its stage of growth, the highest content being at the time of flowering. We report a case of primary irritant dermatitis due to contact with Ranunculus Tachiorei which was applied for the folk treatment of Bell palsy.