Ulinastatin alleviated Lps-induced injury of cardiac micro vascular endothelial Cell via NF-kB pathway

The aim of this study to investigate the effect of ulinastatin [UTI] on lipopolysaccharide [LPS] -induced injury of cardiac micro vascular endothelial cell, and explore potential mechanisms Primary cardiac micro vascular endothelial cells were harvested from neonatal Sprague Dawley rats. Cardiac micro vascular endothelial cells were prepared for further treatment after subculture. The experiment was designed into 4 groups: Control group, LPS [0.1U/ml] group, UTI [100U/ml] group and [UTI+LPS] group. MTT assay and scratch test were performed to assess cell viability of cardiac micro vascular endothelial cells. Flow cytometry was performed to examine apoptosis. Western blot was performed to examine expression of multiple proteins, including pAkt, Bcl-2, NF-kB, TNF-alpha and Caspase-3. Compared with control group, LPS treatment indeed increased protein expression of Caspase-3, and resulted in significant apoptosis of cardiac micro vascular endothelial cells. Compared with LPS group, UTI+LPS group had a higher level of cell viability, verified by MTT assay and scratch test. Moreover, expressions of pAkt, NF-kB and Bcl-2 were decreased after UTI treatment, suggesting UTI significantly alleviated LPS induced-apoptosis of cardiac micro vascular endothelial cell via Akt/NF-kB pathway. Ulinastatin could protect cardiovascular system by alleviating LPS-induced injury of cardiac micro vascular endothelial cell. The potential mechanism is Akt/NF-kB pathway

Cardiac field potentials and activation sequence in Langendorff perfused heart, cardiac tissue slices and cultured ventricular myocytes recorded by microelectrode arrays system / 中华心血管病杂志

<p><b>OBJECTIVE</b>To observe field potentials (FPs) and activation sequence at Langendorff perfused guinea pigs heart, SD rat cardiac tissue strips perfused by Tyrode's solution and cultured ventricular cardiomyocytes of suckling mice by microeletrode arrays (MEA) technique.</p><p><b>METHOD</b>FPs and activation sequence were recorded from perfused heart, cardiac tissue strips (5 mm x 5 mm) and cultured ventricular cardiomyocytes by MEA.</p><p><b>RESULTS</b>(1) FPs could be recorded in hearts perfused for 30 to 90 min with a heart rate 90 - 120 beats/min. FP durations of both ventricular and atrial tissue were (210 +/- 78) ms and (164 +/- 58) ms, respectively and atrial ventricular conduction time was (320 +/- 150) ms. (2) The excitability of Tyrode's solution perfused tissue strips was visible for more than 2 h, and FP durations of ventricular and atrial tissue strips were (115.80 +/- 11.61) ms and (83.71 +/- 6.48) ms, respectively. (3) Spontaneous beating frequency (150 +/- 100) beats/min and FPs could be readily recorded in cardiomyocytes cultured between 2 to 72 hours.</p><p><b>CONCLUSION</b>MEAs is a sensitive, low noise and stable technique for observing local tissue action potential and activation sequence of whole heart, cardiac tissue strips and cultured cardiomyocytes.</p>