Nanotopography and oral bacterial adhesion on titanium surfaces: in vitro and in vivo studies

Abstract The present study aimed to evaluate the influence of titanium surface nanotopography on the initial bacterial adhesion process by in vivo and in vitro study models. Titanium disks were produced and characterized according to their surface topography: machined (Ti-M), microtopography (Ti-Micro), and nanotopography (Ti-Nano). For the in vivo study, 18 subjects wore oral acrylic splints containing 2 disks from each group for 24 h (n = 36). After this period, the disks were removed from the splints and evaluated by microbial culture method, scanning electron microscopy (SEM), and qPCR for quantification of Streptococcus oralis, Actinomyces naeslundii, Fusobacterium nucleatum, as well as total bacteria. For the in vitro study, adhesion tests were performed with the species S. oralis and A. naeslundii for 24 h. Data were compared by ANOVA, with Tukey's post-test. Regarding the in vivo study, both the total aerobic and total anaerobic bacteria counts were similar among groups (p > 0.05). In qPCR, there was no difference among groups of bacteria adhered to the disks (p > 0.05), except for A. naeslundii, which was found in lower proportions in the Ti-Nano group (p < 0.05). In the SEM analysis, the groups had a similar bacterial distribution, with a predominance of cocci and few bacilli. In the in vitro study, there was no difference in the adhesion profile for S. oralis and A. naeslundii after 24 h of biofilm formation (p > 0.05). Thus, we conclude that micro- and nanotopography do not affect bacterial adhesion, considering an initial period of biofilm formation.

Stability of beta-titanium T-loop springs preactivated by gradual curvature

ABSTRACT Objective: Evaluate changes in the force system of T-Loop Springs (TLS) preactivated by curvature, due to stress relaxation. Methods: Ninety TLSs measuring 6 x 10 mm, produced out with 0.017 x 0.025-in TMA® wire and preactived by gradual curvature, were randomly distributed into nine groups according to time point of evaluation. Group 1 was tested immediately after spring preactivation and stress relief, by trial activation. The other eight groups were tested after 24, 48 and 72 hours, 1, 2, 4, 8 and 12 weeks, respectively. Using a moment transducer coupled to a digital extensometer indicator adapted to a universal testing machine, the amount of horizontal force, moment and moment-to-force ratios were recorded at every 0.5 mm of deactivation from 5 mm of the initial activation, in an interbracket distance of 23 mm. Results: The horizontal forces decreased gradually among the groups (p< 0.001) and the moments showed a significant and slow decrease over time among the groups (p< 0.001). All groups produced similar M/F ratios (p= 0.532), with no influence of time. Conclusions: The TLSs preactivated by curvature suffered a gradual deformation over time, which affected the force system, specifically the moments, which affected the horizontal forces produced.

RESUMO Objetivo: avaliar as mudanças no sistema de forças das molas T pré-ativadas por curvatura, devido ao alívio de tensão estrutural. Métodos: noventa molas em forma de "T", medindo 6 mm x 10 mm, confeccionadas com fio de beta-titânio TMA® de 0,017" x 0,025" e pré-ativadas por curvatura gradual, foram distribuídas aleatoriamente em nove grupos, de acordo com o momento de avaliação. O Grupo 1 foi testado imediatamente após a pré-ativação da mola e liberação do estresse, por meio de simulação da ativação. Os outros oito grupos foram divididos conforme o intervalo de tempo testado: após 24, 48 e 72 horas; 1, 2, 4, 8 e 12 semanas. Usando um transdutor de momentos acoplado a um indicador digital para extensometria e adaptado a uma máquina universal de ensaios, as magnitudes da força horizontal, do momento e da proporção momento/força (M/F) foram registradas a cada 0,5 mm de desativação a partir da ativação inicial de 5 mm, utilizando-se uma distância interbraquetes de 23 mm. Resultados: as forças horizontais reduziram-se gradualmente nos grupos (p< 0,001) e o momento apresentou uma queda lenta e significativa ao longo do tempo (p< 0,001). Todos os grupos tiveram proporções M/F semelhantes (p= 0,532), não influenciadas pelo período de tempo. Conclusões: as molas pré-ativadas por curvatura gradual sofreram deformação progressiva ao longo do tempo, o que afetou o sistema de forças - especificamente o momento -, alterando as forças horizontais produzidas.

Campomanesia velutina leaves extracts exert hypouricemic effects through inhibition of xanthine oxidase and ameliorate inflammatory response triggered by MSU crystals

ABSTRACT Gout is a destructive arthritis with a high prevalence worldwide. However, the available therapy is not able to increase life quality in many patients. Campomanesia velutina (Cambess) O. Berg, Myrtaceae, is used in Brazilian folk medicine to treat pain, inflammation and rheumatism. The aim of this study was to evaluate the potential of ethanolic and aqueous extracts from C. velutina leaves to treat hyperuricemia and inflammation in gout arthritis model. Ethanolic extract of leaves and aqueous extract of leaves were in vitro assayed on xanthine oxidase inhibitory effect and in vivo on an experimental model of oxonate-induced hyperuricemia in mice, liver xanthine oxidase inhibition and monosodium urate crystal-induced paw edema model. The extracts at both tested doses (100 and 300 mg/kg) reduced serum urate levels. They were also able to inhibit xanthine oxidase in vitro and in vivo, demonstrating that this might be the mechanism of action underlying the urate-lowering effects. In addition, the extracts showed significant anti-inflammatory activity on monosodium urate crystal-induced paw edema, especially aqueous extract (100 and 300 mg/kg) that reduced edema at all evaluated times. Rutin and myricitrin were identified in ethanolic and in aqueous extracts. In this study, myricitrin was able to reduce serum uric acid levels and inhibit liver xanthine oxidase at the dose of 15 mg/kg. The anti-hyperuricemic activity of rutin has been previously reported. Thus, rutin and myricitrin seem to contribute to the observed effects of ethanolic and aqueous extracts. The results demonstrated the ability of aqueous and ethanolic extracts to lower serum urate levels and to reduce edema induced by monosodium urate crystals. Therefore, they may contribute to the management of gout in the future.

Effects of Initial Periodontal Treatment in Moderately Compensated and Decompensated Type 2 Diabetic Patients.

Objective: The objectives of this research were to evaluate the effect of periodontal therapy in moderately compensated and decompensated type 2 diabetic patients. Materials and Methods: 20 patients with type 2 diabetes mellitus (DM2) and periodontal disease were selected and divided into two groups (based on HbA1c level): Group 1: Moderately compensated; Group 2: Decompensated. The analyses including clinical periodontal parameters and the quantification of gingival crevicular fluid (GCF), the IL1-β expression in the GCF and Glycated hemoglobin (HbA1c) and fasting glucose (FG) from venous blood were performed at 0, 3, and 6 months. Results: Both groups presented improvement in all clinical periodontal parameters as well as quantification of gingival crevicular fluid and in the expression of IL-1ß present in the fluid after 6 months. However, no statistically significant difference was found in the levels of HbA1C in the group 1 after 6 months, although a significant increase was found after six months in the group 2. While in relation to the FG, a great improvement was found in the group 1 and a significant increase in the group 2 after six months. Conclusions: It was possible to observe that conventional periodontal treatment (scaling and root planning) is more effective for moderately compensated type 2 diabetic patients’ glycemic control rather than for the decompensated patients.

Oferta do teste sorológico para hepatite B durante o pré-natal: a vivência das puérperas / Serological test for hepatitis B offered during antenatal care: the women’s experience / Oferta de prueba serológica para hepatitis B durante el prenatal: la vivencia de las puérperas

Com a finalidade de reduzir os níveis de infecção pela transmissão vertical da Hepatite B, é necessário acompanhamento mais eficaz durante pré-natal. Objetivou-se identificar como ocorreu a oferta do teste sorológico para a Hepatite B durante o pré-natal, segundo as puérperas. A pesquisa é quantiqualitiva e utilizou-se discurso do sujeito coletivo. Foi realizada no alojamento conjunto em uma maternidade escola no Rio de Janeiro. Foram entrevistadas 41 puérperas de janeiro a março de 2011. Quando questionadas sobre oferta do teste, a maioria respondeu que foi solicitado junto com os exames de rotina do pré-natal, o profissional de saúde apenas leu o resultado. Mesmo havendo recomendação do Ministério da Saúde para oferta e realização do teste sorológico para Hepatite B, há um déficit na forma como é abordado o tema com a mulher.

In order to reduce B Hepatitis vertical transmission, more effective antenatal care is necessary. Objectives: to identify how, from the women’s standpoint, the serologic test for Hepatitis B was offered during antenatal care. This quantiqualitative study using collective subject discourse was conducted at the rooming-in ward of a maternity hospital school in Riode Janeiro, Brazil. Between January and March 2011, 41 puerperal women were interviewed. When asked how the test was offered, most answered that it was requested together with the other routine antenatal tests; health professionals merely read the result. Despite the Ministry of Health recommendation that Hepatitis B testing be offered and performed, therre shortfall in the way the subject is addressd treated with women about to give birth.

Con el fin de reducir los niveles de infección por la transmisión vertical de la Hepatitis B, es necesario acompañamiento más eficaz durante el prenatal. Se procuró identificar como se produjo la oferta de la prueba serológica para Hepatitis B durante el prenatal, según las puérperas. La investigación es cuantitativa y se empleó discurso del sujeto colectivo. Se realizó en el alojamiento conjunto en una maternidad escuela en Río de Janeiro. Se entrevistaron 41 puérperas entre enero y marzo de 2011. Cuando interrogadas sobre la oferta de la prueba, la mayoría respondió que fue solicitado junto a las pruebas de rutina del prenatal, el profesional sanitario apenas leyó el resultado. Incluso habiendo recomendación del Ministerio de Sanidad para oferta y realización de la prueba serológica para Hepatitis B, hay un déficit en la forma de abordaje del tema con la mujer.

Avaliação da qualidade dos tratamentos endodônticos em centros de especialidades odontológicas da Grande Natal - RN / Quality of endodontic treatments at dental specialty centers in the Greater Natal, RN, Brazil

Objetivo: Avaliar a qualidade técnica dos tratamentos endodônticos realizados em Centros de Especialidades Odontológicas (CEOs) da Grande Natal/RN no período de 2006 a 2008. Método: Estudo transversal com amostra de 282 dentes submetidos a tratamento endodôntico há pelo menos um ano. Os participantes da pesquisa foram avaliados através de exames clínicos e radiográficos. Os dentes tratados endodonticamente foram examinados por uma especialista em endodontia, que comparou e analisou as radiografias realizadas ao final do tratamento e anexadas ao registro do paciente no CEO com tomadas radiográficas atuais. Os dados coletados foram apresentados de forma descritiva através de números absolutos, percentuais e médias. Para a determinaçÆo da associaçÆo entre as variáveis independentes e as variáveis dependentes realizou-se análise bivariada através do teste do qui-quadrado e exato de Fisher. Resultados: Dos elementos dentários examinados 79,8% apresentaram-se sem lesÆo periapical, 84,4% sem dor, 8,2% fraturados e 3,2% extraídos. As obturaçães dos canais encontravam-se adequadas em 81,2%. Com relaçÆo ao limite longitudinal, 135 (47,9%) das obturaçães foram consideradas com limite ideal, 50 (17,7%) sobreobturados e 88 (31,2%) subobturados. A persistência de lesÆo periapical esteve associada ao estado periapical inicial (p<0,05). ConclusÆo: Os tratamentos endodônticos que foram realizados nos Centros de Especialidades Odontológicas possuem uma adequada qualidade técnica, resultando no sucesso da terapia endodôntica realizada nestes Centros...

Objective: To evaluate the technical quality of the endodontic treatments performed at Dental Specialty Centers (DSC) in the Greater Natal, RN, Brazil, between 2006 and 2008. Method: A cross-sectional design was used with a sample of 282 teeth with endodontic treatments completed at least 1 year before the study. The participants were subjected to clinical and radiographic. The endodontically treated teeth were examined by an endodontist who compared and analyzed the radiographs taken at the end of the treatment as part of the patient?s documentation at the DSC with the current radiographs. The collected data were presented by descriptive statistics in absolute numbers, percentages and means. Association among the independent and dependent variables was made by bivariate analysis with the chi-square and Fisher?s exact tests. Results: As much as 79.8% of the teeth did not present periapical lesion, 84.4% had no associated painful symptomatology, 8.2% were fractured and 3.2% had been extracted. The root fillings were adequate in 81.2% of the teeth. Regarding the apical limit of root canal filling, adequate filling was observed in 135 teeth (47.9%), overfilling in 50 (17.7%) and underfilling in 88 (31.2%). Persistence of periapical lesion was associated with the initial stage of apical periodontitis (p<0.05). Conclusion: The endodontic treatments performed at the DSC have adequate technical quality, resulting in success of the root canal therapy...

Circulation of the rabies virus in non-hematophagous bats in the city of Rio de Janeiro, Brazil, during 2001-2010 / Circulação do vírus da raiva em morcegos não-hematófagos na cidade do Rio de Janeiro no período entre 2001-2010

INTRODUCTION: Rabies is one of the most known lethal zoonosis, responsible for 55,000 human deaths per year. It is transmitted to humans mainly by the bite of domestic or wild animals infected with the virus. This paper shows the circulation of this virus in non-hematophagous bats in the City of Rio de Janeiro, Brazil. METHODS: A survey was performed on the number of bats that had been sent for diagnosis by the Seção de Virologia of the Instituto Municipal de Medicina Veterinária Jorge Vaitsman and were positive for rabies. The positive animals were identified, and the isolated viruses were sent for antigenic typification with indirect immunofluorescence. The results were compared with the antigenic panel of the Centers for Disease Control and Prevention. RESULTS: During 2001-2010, the laboratory received 555 non-hematophagous bats for rabies diagnosis, with 198 (35.7%) from Rio de Janeiro City. A total of 11 (5.5%) animals were positive for this disease. Antigenic typification revealed the predominance of variant 3 in 9 (81.8%) of the isolated viruses; 1 virus was classified as variant 4 and 1 variant was identified that segregated with the viruses in insectivorous bats. CONCLUSIONS: The data obtained in this study showed the presence of the rabies virus in synanthropic populations of non-hematophagous bats in the City of Rio de Janeiro. The circulation of this agent in these animals represents a serious risk to human and animal health and requires attention and control measures by the authorities.

INTRODUÇÃO: A raiva é uma das mais letais zoonoses conhecidas, responsável pelo óbito de 55 mil pessoas anualmente. É transmitida ao homem principalmente pela mordida de animais, domésticos ou silvestres, infectados pelo vírus. O presente estudo mostra a circulação deste agente em morcegos não-hematófagos no município do Rio de Janeiro, Brasil. MÉTODOS: Na Seção de Virologia do Instituto Municipal de Medicina Veterinária Jorge Vaitsman, foi realizado um levantamento do número de morcegos recebidos positivos para o diagnóstico. Os animais positivos foram identificados, e o vírus isolado foi submetido à tipificação de variantes antigênicas pela técnica de imunofluorescência indireta, com os resultados comparados com o painel antigênico utilizado pelo Centro de Controle de Doenças (Center of Disease Control -CDC). RESULTADOS: Entre 2001-2010, o laboratório recebeu 555 morcegos nãohematófagos para o diagnóstico da raiva, sendo 135 (35,5%) do município do Rio de Janeiro. Um total de 11 (5,5%) animais foram positivos para a doença. A tipificação antigênica revelou a predominância da variante 3, com nove (81,9%) vírus isolados, um pertencente a variante 4 e outro de uma variante que segrega com a presente em morcegos insetívoros. CONCLUSÕES: Os dados observados no presente estudo demonstraram a presença do vírus da raiva em populações sinantrópicas de morcegos não-hematófagos no município do Rio de Janeiro. A circulação deste agente nesses animais representa um grave risco a saúde humana e animal, e requer atenção e medidas de controle e prevenção por parte das autoridades competentes.

Neuroblastoma intrarenal mimetizando tumor de Wilms / Intrarenal neuroblatoma mimics Wilms' tumor

No presente trabalho, os autores relatam o caso de uma criança com neuroblastoma intrarenal, que foi, inicialmente,diagnosticado como tumor de Wilms. Pré-escolar, sexo feminino, com um ano e três meses, apresentava uma tumoração endurecida que ocupava o hipocôndrio esquerdo e se estendia até a região do mesogástrio, acompanhada de febre e palidez. O ultra-som do abdome total revelou massa intrarenal. A biópsia por agulha fina, em vários pontos de acesso tumoral, revelou um tumor de Wilms. Entretanto, não foi possível naquele momento realizar a imunohistoquímica (IHQ), face à escassez de material. Diante da gravidade da paciente, foi iniciado o protocolo SIOP por quatro semanas. Como não houve resposta clínica, foi indicada uma laparotomia exploradora, com ressecção parcial do tumor, sendo também, nesse momento, realizada punção aspirativa de medula óssea (MO). O exame histopatológico revelou neoplasia maligna de pequenas células mal diferenciadas. A IHQ foi negativa para WT-1 e positiva para NB-84, cromogranina e sinaptofisina. A biologia molecular revelou amplificação de N-myc. O mielograma identificou infiltração medular por pequenas células redondas. O neuroblastoma intrarenal é um tumor raro que se assemelha clínica e radiologicamente ao tumor de Wilms. Esse trabalho procura enfatizar a importância do emprego de análises imunohistoquímica e moleculares para o diagnóstico do neuroblastoma intrarenal.

This work reports the case history of a child with intrarenal neuroblastoma, initially diagnosed as Wilms' tumor.The patient, a one year and three months old girl, presented a hard abdominal mass on the left flank that extended to the mesogastric region, plus fever and paleness. The ultrasound of the entire abdomen revealed an intrarenal mass. Biopsy with fine needle in many points of the tumor revealed Wilms' tumor. The scarcety of the material, however, made immunohistoquemistry impossible at that moment. Because of the child's severe condition the SIOP protocol was started. As no clinical response was observed, an exploratory laparatomy was indicated with partial resection of the tumor and bone marrow aspiration (MO). The histopathologic study revealed a malignant neoplasia of small cells, poorly differentiated. IHQ was negative for WT-1 and positive for NB-84, synaptofisin, cromogranine. N-myc amplification was observed by molecular biology. The bone marrow aspiration identified matastatic small round cells infiltration. Intrarenal neuroblastoma is a rare entity that clinically and radiographicallyresembles Wilms' tumor. The objective of this case report is to show the importance of immunohistochemical andmolecular analysis in the diagnosis of intrarenal neuroblastoma.