RÉSUMÉ
OBJECTIVE: To investigate the changes of extraction rates of forsythiaside A and forsythin in Forsythia suspensa compatible with other medicinal material of Menshi huwei formula before and after decoction. METHODS: HPLC method was used to determine the extraction amounts and to calculate the extraction rates of forsythiaside A and forsythin in F. suspensa (5 g×7 doses), F. suspensa (5 g×7 doses) compatible with Pinelliae rhizoma praeparatum cum zingibere et alumine (PRZA), Menshi huwei formula [including 6 ingredients as F. suspense (5 g×7 doses), PRZA] after decocted with water. The determination was performed on Diamonsil C18 column with mobile phase consisted of acetonitrile-0.2% formic acid solution (gradient elution). The detection wavelength was set at 278 nm, and the column temperature was 30 ℃. The flow rate was 1.0 mL/min. RESULTS: The linear range of forsythiaside A and forsythin were 0.61-6.1, 0.246-2.46 μg (r=0.999 7, 0.999 9), respectively; RSDs of precision, stability (within 20 h) and reproducibility tests were all lower than 2% (n=6). Average recovery rates were 96.10%-99.37% (RSD≤2.36%,n=6) respectively. In F. suspensa, extraction rates of forsythiaside A and forsythin were 96.90% and 66.67%. In F. suspensa compatible with PRZA, extraction rates of them were 101.61% and 54.55%. In Menshi huwei formula, extraction rates of them were 98.39% and 84.85%. CONCLUSIONS: After F. suspensa is compatible with PRZA, the extraction rates of forsythiaside A is increased while forsythin is decreased. After compatible with other medicinal material in Menshi huwei formula, extraction rates of both are increased slightly.