To Explore the Effect and Mechanism of Shenghui Granule on EC and CA1 Regions of AD Model Rats Based on p38 MAPK Signal Pathway / 世界科学技术-中医药现代化

Objective To observe the effect of Shenghui Granule on EC and CA1 regions of scopolamine-induced dementia rats and explore its mechanism based on p38 MAPK signal pathway.Methods 40 SD rats were randomly divided into four groups(blank group,model group,Shenghui granule group,donepezil group),and were treated with scopolamine.Morris water maze and open field test were used to evaluate the cognition and anxiety behavior of rats.The nerve injury of EC and CA1 was observed by HE staining.The activity of neurons in EC and CA1 regions was observed by c-Fos immunofluorescence staining.Western blot was used to detect p38 MAPK pathway related proteins.Results The behavioral experiment found that Shenghui Granule could improve the cognitive impairment and anxiety-like behavior of AD model rats.The results of HE staining showed that Shenghui granules had protective effects on EC and CA1 regions.The results of c-Fos immunofluorescence staining showed that Shenghui granules could increase the activity of neurons in EC and CA1 regions.Western blot results showed that Shenghui Granule could down-regulate the expression of Bax,reduce the levels of phosphorylated p38 and Tau,and increase the expression of Bcl-2.Conclusion Shenghui granule has protective effect on EC and CA1 regions of AD model rats,and may play a therapeutic role through p38 MAPK signal pathway.

Protective effect of Acronychia pedunculata water extract on photoaging keratinocyte model and its mechanism / 中华医学美学美容杂志

Objective:To investigate the protective effect and mechanism of Acronychia pedunculata water extracts on UV-induced light damage of human keratinocytes.Methods:The experiment was conducted from December 2018 to April 2020 in the Guangxi Medical University Laboratory of Genetics. The photoaged keratinocyte model was used, the cells were co-cultured with different concentrations of Acronychia pedunculata water extracts. The cell proliferation rate was detected by CCK-8 method. The levels of reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total antioxidant capacity (T-AOC) of cells were detected by a test kit. The levels of IL-1β, IL-6 and tumor necrosis factor-alpha (TNF-α) were determined by ELISA.Results:The proliferation of HaCaT cells was promoted by 0.5 mg/L-2.0 mg/L of the extracts. Compared with control group, the proliferation rate of HaCaT cells in the experimental group was significantly increased ( P<0.05). Compared with control group, the contents of ROS was decreased ( F=214.67, P<0.05), MDA was decreased ( F=811.88, P<0.05), SOD was increased ( F=28.95, P<0.05), CAT was increased ( F=213.31, P<0.05), GPX was increased ( F=65.10, P<0.05), T-AOC was increased ( F=305.58, P<0.05), IL-1β was decreased ( F=15.46, P<0.05), IL-6 was decreased ( F=59.2, P<0.05), and TNF-α was decreased ( F=33.13, P<0.05). Conclusions:The extracts of 0.5-2.0 mg/L of Acronychia pedunculata have protective effects on the photoaging cell model, which may be related to the increase of SOD, CAT, GPX and other antioxidant enzymes and the level of T-AOC in photoaging HaCaT cells, and the decrease of ROS, MDA content and the expression of inflammatory cytokines.

Intestinal microbiome changes in patients with postpartum depression / 西安交通大学学报(医学版)

【Objective】 To analyze the changes of gut microbes in patients with postpartum depression so as to explore the relationship between postpartum depression and gut microbes. 【Methods】 A total of 60 postpartum subjects were recruited to participate in this study. The depression status of the participants was scored using the Edinburgh Postpartum Depression Scale (EPDS). Those with a score ≥13 were included in the postpartum depression group (PPD group), while those with a score less than 13 were included in the postpartum healthy control group (PPHC group). The feces of these 60 subjects were collected, and the fecal whole genome DNA was extracted for 16S rDNA sequencing. The data of changes in the bacterial diversity between the groups were obtained, and the possible correlation between the changes of intestinal microbes and postpartum depression was analyzed. 【Results】 The number of microorganisms in PPD patients was significantly reduced (P<0.001); the Chao1 index (P<0.001) and ACE index (P<0.001) of α diversity decreased significantly. There were also significant differences in β diversity between the two groups. Analysis of the bacteria in the groups showed that Acetanaerobacterium, Adlercreutzia, Allobaculum, Alloprevotella, Bifidobacterium, Christensenella, Escherichia, Eubacterium, Faecalicatena, Fusobacterium, Haemophilus, Intestinimonas, Lactobacillus, Megamonas, Monoglobumus, Muribaculum, Oscillospira, Paraprevotella, Streptococcus, Raoultibacter, Ruminococcus and Stomatobaculum were significantly enriched in PPHC group. In contrast, Kineothrix, Lachnoclostridium, Acinetobacter, Aquisphaera, Enterococcus, and Mucispirillum were enriched in PPD group. RDA/CCA analysis showed that EPDS was positively correlated with Prevotella, Kineothrix, and Alistipes, but negatively correlated with Lachnospira. 【Conclusion】 This study found that the intestinal flora of patients with postpartum depression was significantly disrupted, and there was a correlation between the intestinal flora and postpartum depression symptom score. Therefore, intestinal microbial markers may contribute to the diagnosis and treatment of patients with postpartum depression.

Correlation of serum inflammatory factors with clinical symptoms and cognitive functions in schizophrenia / 西安交通大学学报(医学版)

【Objective】 To study the correlation of clinical symptoms and cognitive functions with serum inflammatory factors in schizophrenia. 【Methods】 A total of 42 SCz patients (case group) and 47 healthy controls (control group) were enrolled in this study. We used enzyme-linked immunosorbent assay (ELSA) to determine six inflammatory factors in serum. PANSS was used to assess clinical symptoms and MCCB was used to assess the patients’ cognitive functions. 【Results】 ① Inflammatory factors: The serum levels of IL-1β, IL-4, IL-6 and IL-8 were significantly higher in case group than in control group (P<0.01). ② Cognitive functions: The scores of Trail Making Test, Hopkins Verbal Learning Test, Symbol Coding, Spatial Span, Brief Visuospatial Memory Test, Assessment Battery-Mazes, Category Fluency and Test-Managing Emotions of case group were significantly lower than those of control group (P<0.05). ③ Correlation between serum inflammatory factors and clinical symptoms: There was no correlation between serum inflammatory factors and psychiatric symptoms in schizophrenia. ④ Correlation between serum inflammatory factors and cognitive functions: The levels of IL-6 (rs=-0.33, P<0.05) and IL-8 (rs=-0.50, P<0.01) in case group were significantly negatively correlated with the scores of Space Scan test. 【Conclusion】 Patients with schizophrenia are presented with immune dysfunction, and the latter is correlated with cognitive impairments.

Comparison of the osteogenic effect of three different bone graft materials / 中国组织工程研究

BACKGROUND: Dentin particles, tricalcium phosphate/collagen protein composites and Bio-oss particles can repair jaw defects, but the excellent osteogenic effect is not clear. OBJECTIVE: To compare osteogenic effects of three different bone graft materials on mandibular defects in beagle dogs. METHODS: Eight 1-year-old beagles were selected. A boxed bone defect area of 10 mm × 8 mm × 2 mm was prepared at the bilateral mandibular external oblique line and randomly divided into four groups with four bone defect models in each group. Undemineralized dentin particles were implanted in group A; tricalcium phosphate/ collagen protein composite materials were implanted in group B; Bio-oss particles were implanted in group C; and group D was used as blank control. Three months after transplantation, the samples were taken for histological observation. The experimental animals were approved by the Ethics Committee of the Experimental Animal Center of Dalian Medical University. RESULTS AND CONCLUSION: (1) In group A, dentin particles were slightly absorbed, surrounded by new bone tissue; new bone trabeculae and capillaries could be seen, and a large number of fibrous connective tissue surrounded dentin particles in the central area of bone graft. In group B, a small number of new bone trabeculae and osteoblasts could be seen; a large number of powdered β-tricalcium phosphate particles and a small amount of inflammatory cells could be seen in the fibrous connective tissue; and some β-tricalcium phosphate particles were surrounded by new bone tissue. Bone marrow cavity could be seen in the new bone. In group C, some Bio-oss particles were surrounded by new bone tissue; some Bio-oss particles were wrapped by surrounding fibrous connective tissue, and fibers, particles and new bone were intertwined. There was no new bone formation in group D, and many capillaries could be seen in a large number of fibrous connective tissue. (2) The rate of new bone formation in groups A, B and C was higher than that in group D (P < 0.05); the rate in groups A and C was higher than that in group B (P < 0.05). (3) The results show that all the three kinds of bone graft materials can promote the formation of new bone. The short-term osteogenic effects of undecalcified xenogeneic dentin particles and Bio-oss particles are better than tricalcium phosphate/collagen protein composites, but the long-term effects need to be further observed.

Effect of Hospital - community Integration Follow - up on Medication Compliance in Patients with Mental Disorder / 现代医院

Objective To explore the effect of hospital-community integration follow-up pattern on medication compliance in patients with mental disorder. Methods From January 2016 to January 2017, 96 patients were treated effectively with systemic therapy in psychosomatic department and TCM WM department, and 96 discharged mental disorder patients were treated with brief psychiatric rating scale (BPRS) ≤ 28. The patients were randomly divided into 48 cases in the intervention group and the control group; both groups were standardized medication; control group received an outpatient referral, intervention group combined with medical doctors in our hospital and community doctors for one year follow-up intervention. Observed the condition of illness and medication compliance after six months and one year intervention between two groups, compared the patient recurrence rate of one year after discharge. Results BPRS score of intervention group was significantly lower than control group (P＜0. 01), the difference was statistically significant, while intervention group had higher compliance than control group (P＜0. 01), and the difference was statistically significant. Recurrence rate (20. 83％) of intervention group was significantly lower than control group (38. 78％) ( 2 = 4. 03, P＜0. 05), the difference was statistically significant. Conclusion Hospital-community integration follow-up has positive effect on stabilizing patients with mental disorder and improving compliance and decreasing relapse rate.

The investigation of the Patients' condition in maintenance hemodialysis and the tumor occurrence / 中国综合临床

Objective To investiGate the constituent ratio of etioloGy,complications and tumor occurrence of maintenance hemodialysis patients. Methods Obseved the patients with maintenance hemodialysis in ZhonGshan Hospital Affiliated to Dalian University in 20l3. The patientsˊ aGe,Gender, protopathy,and other basic information includinG statistics complication and tumor occurrence rate were surveyed. Results There was same ratio of male and female in 222 cases. The primary disease of diabetic nephropathy accounted for 30. 2%( 67 cases ),hypeertensive nephropathy 2l. 2%( 47 cases ),chronic Glomerulonephritis l8. 0%(40 cases),polycystic kidney 6. 8%(l5 cases),obstructiverenal injury accounted for 5. 4%(l2 cases)and other diseases. HemoGlobin of 73 cases(32. 0%)reached the tarGet value(lll G/L) . There was 55. 2% of patients ( l23 cases ) experienced calcium and phosphorus metabolism disorder. Parathyroid hormone levels of 47. 4% patients( l47 cases ) were hiGher than normal. The tumor incidence was 7. 6%( l7/222 ). Conclusion The major primary disease of the patients with maintenance hemodialysis are diabetic nephropathy,hypertension nephropathy and chronic Glomerulonephritis. The level of hemoGlobin,calcium and phosphorus metabolism disorder and parathyroid hormone are low. There is hiGh incidence of tumor.