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Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;49(12): e5519, 2016. tab, graf
Article de Anglais | LILACS | ID: biblio-828177

RÉSUMÉ

The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.


Sujet(s)
Humains , Mâle , Femelle , Adulte d'âge moyen , Infections à Chlamydia/sang , Infections à Chlamydia/génétique , Valvulopathies/microbiologie , Lectine liant le mannose/sang , Lectine liant le mannose/génétique , Études cas-témoins , Infections à Chlamydia/diagnostic , Études transversales , Fréquence d'allèle , Prédisposition génétique à une maladie , Génotype , Valvulopathies/sang , Valvulopathies/chirurgie , Réaction de polymérisation en chaîne , Polymorphisme de nucléotide simple
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