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Mem. Inst. Oswaldo Cruz ; 115: e200339, 2020. tab, graf
Article de Anglais | LILACS | ID: biblio-1154865

RÉSUMÉ

We evaluated sweat, blood and urine specimens obtained from an ongoing cohort study in Brazil. Samples were collected at pre-established intervals after the initial rash presentation and tested for Zika virus (ZIKV) RNA presence by real-time reverse transcriptase polymerase chain reaction (rRT-PCR). From 254 participants with confirmed infection, ZIKV RNA was detected in the sweat of 46 individuals (18.1%). Sweat presented a median cycle threshold (Ct) of 34.74 [interquartile range (IQR) 33.44-36.04], comparable to plasma (Ct 35.96 - IQR 33.29-36.69) and higher than urine (Ct 30.78 - IQR 28.72-33.22). Concomitant detection with other specimens was observed in 33 (72%) of 46 participants who had a positive result in sweat. These findings represent an unusual and not yet investigated virus shedding through eccrine glands.


Sujet(s)
Humains , Mâle , Femelle , Adulte , Sueur/virologie , ARN viral/génétique , Virus Zika/isolement et purification , Infection par le virus Zika/diagnostic , Urine/virologie , Sang/virologie , Brésil/épidémiologie , ARN viral/isolement et purification , ARN viral/classification , Études de cohortes , RT-PCR , Réaction de polymérisation en chaine en temps réel , Virus Zika/génétique , Infection par le virus Zika/épidémiologie
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