Molecular mechanism of Shengmaiyin for arrhythmia based on integrative pharmacology / 药学学报

Shengmaiyin is widely used in the treatment of arrhythmia and has achieved a good effect. Due to the complexity of traditional Chinese medical formula, the pharmacological mechanism of Shengmaiyin in the treatment of arrhythmia is unclear. In this study, we used the internet-based Computation Platform (www.tcmip.cn) to explore the molecular mechanism. Shengmaiyin was found to treat the arrhythmia by modulating the pathway related to energy metabolism such as carbon metabolism, purine metabolism, carbohydrate metabolism, or by regulating the level of ATP. In this study, we find that the main active drug component in Shengmaiyin may be ginseng.

Studies on HPLC-fingerprint of Rhizoma Fagopyri Dibotoryis / 中国中药杂志

<p><b>OBJECTIVE</b>To establish an HPLC fingerprint of Rhizoma fagopyri dibotoryis.</p><p><b>METHOD</b>The HPLC-electrochemical detection assay was used to establish the fingerprint of Rhizoma fagopyri dibotoryis. The sample was performed on a column of Diamonsil C18 (4.6 mm x 250 mm, 5 microm) which was eluted with methanol- 0.1 mol x L(-1) phosphate buffer (pH 2.5), the flow rate was 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the work electrode was glassy carbon, the counter electrode was Pt platmun.</p><p><b>RESULT</b>The HPLC fingerprint profiles of 6 Rhizoma fagopyri dibotoryis contains 6 common chromatographic peaks, and gallic acid, protocatechuic acid, protocatechuic aldehyde and ( - ) -epicatechin were tested the samples. The contents of protocatechuic acid and protocatechuic aldehyde were from 0.004% to 0.05% and from 0.003% to 0.015%, respectively.</p><p><b>CONCLUSION</b>The method can be used to control the quality of Rhizoma fagopyri dibotoryis.</p>

Key influencing factors on essential oil components of Atractylodes lancea and study on its division of climate adaptability / 中国中药杂志

<p><b>OBJECTIVE</b>To find the key factors that influence the content of the essential oil components of Atractylodes lancea and classify the habitat of A. lancea based the essential oil components, as well as explore a new method to conduct division of Chinese natural medicin through geographic information system (GIS) ultimately.</p><p><b>METHOD</b>The key factors were obtained by canonical correlation and stepwise regressive analysis. Then the interpolation of climatic data for 30 years (1971-2000) was done by Sufer 7.0, and the habitat division was done by ARCGIS 9. 0.</p><p><b>RESULT</b>There were 6 correlation models between climate factors and 6 main essential oil componets were obtained, and a division map about essential oil components content of A. lancea base on clamate adaptability were drawn. It was found that the climate condition in Octobers played a key role on forming the essential oil components and the interaction of tempreture and precipitation was the most important factor on forming the essential oil components of A. lancea.</p><p><b>CONCLUSION</b>The adaptive habitat for forming the essential oil components of A. lancea was different from growing A. lancea ones. And GIS can be used perspective for division of Chinese natural medicine.</p>

Microwave sintering of nanometer powder of alumina and zirconia-based dental ceramics / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The objective of the present study was to investigate the feasibility and reliability of sintering alumina and zirconia-based all-ceramic materials through a recently introduced microwave heating technique. The variation of crystal phases, the growth of grain sizes and microstructural features of these materials were evaluated after sintering.</p><p><b>METHODS</b>Four different groups of powder (l00%Al2O3, 60%Al2O3+40%ZrO2, 40% Al2O3+60%ZrO2, 100% ZrO2) were respectively press-compacted to fabricate green disk samples, 5 specimen of each group were prepared. All the samples were surrounded by refractory materials for heat containment and processed at 1 600 degrees C in a domestic microwave oven (850 W, 2 450 MHz), 1 600 degrees C/5 min for heating rate, 10 min for holding time. After sintering, the phase composition and average grain size of these ceramics were examined using X-ray diffraction (XRD). Their microstructure characteristics were studied by scanning electron microscopy (SEM).</p><p><b>RESULTS</b>All the specimens were successfully sintered with the application of microwave heating system in combination with a suitable thermal insulator. No phase change was found in alumina while monoclinic-zirconia was found to be transformed to tetragonal-zirconia. A little grain size growth of Al2O3 and ZrO2 has been observed with Al2O3 24.1 nm/before and 51.8 nm/after; ZrO2 25.3 nm/before and 29.7 nm/after. The SEM photos indicated that the microwave-sintered Al2O3-ZrO2 ceramics had a uniform crystal distribution and their crystal sizes could be maintained within the range of nanometers.</p><p><b>CONCLUSION</b>It is expected that in the near future microwave heating system could be a promising substitute for conventional processing methods due to its unparalled advantages, including more rapid heating rate, shortened sintering time, superfine grain size, improved microstructure and much less expensive equipment.</p>

Studies on GC fingerprint of volatile oil of Houttuynia cordata / 中国中药杂志

<p><b>OBJECTIVE</b>To establish a GC fingerprint of the volatile oil of Houttuynia cordata.</p><p><b>METHOD</b>The volatile oil was extracted from H. cordata by water stream distillation method, and analyzed by GC coupled with FID.</p><p><b>RESULT</b>12 bathes of samples collected from different regions were analyzed; the GC fingerprint of the volatile oil of H. cordata was subsequently established.</p><p><b>CONCLUSION</b>The established GC fingerprint can be used for the identification of H. cordata.</p>

DNA Immunization of Hepatitis E Virus (HEV) / 中国病毒学

Two plasmid constructs, pcE2 and pcE3, containing 3' fragment of open reading frame 2 (ORF2,1163 bp) of hepatitis E virus (HEV) and full-length ORF3 (369 bp), were injected into bilateral tibialis of Swiss mice respectively，for three times (0, 2nd and 4th weeks) and observed the HEV IgG by ELISA. HEV IgG was induced after the injection of pcE2 or pcE3 or both, and the percentage of seraconversion was 100% after two weeks of the third injection. Compared with injection of either construct, the antibody titers were higher in the group with combined injection of two constructs.