RÉSUMÉ
Object To show that ELSD is an excellent detector for the detection of chemical compounds devoid of chromophore, such as sarsasapogenin Methods Sarsasapogenin in crude Anemar rhena asphodeloides Bunge and its preparation was determinated by RP HPLC ELSD Results The well separated chromatographic peaks show linearity with recovery of the added sample of 100 5% in crude medicinal material and 91 38% in its preparation, r= 0 999 0 Conclusion The method was advanced, reliable, simple and can be used for quality control of crude A asphodeloides and its preparations
RÉSUMÉ
Object To explore the regularity of microwave extraction (ME) on Chinese medicines in different morphological structure and different polar compositions. Methods Anthraquinone in Radix et Rhizoma Rhei (RRR), Semen Cassiae (SC), cholorogenic acid in Flos Lonicerae, baicalin in Radix Scutellariae were determined as index compositions by HPLC. The extraction rate was measured by orthogonal design. Results ME selectivity to different anthraquinone in RRR is not significant, while at the same temperature, the extraction rates of emodin, chrysophanol, physcion in RRR are higher than those in SC. Conclusion The ME selectivity to the different morphological structure of Chinese medicines is obvious, but to the different polar compositions is not distinct.
RÉSUMÉ
Objective: To evaluate the characteristic and explore the mechanism of microwave extraction (MAE) in extracting Chinese medicines by comparing with conventional extractions on Radix et Rhizoma Rhei. Methods : The sum of anthraquinone was determined by spectrophotometry and aphrostase in paraffin section was observed by microphotography. Results : Among the four methods, the efficiency of MAE was significantly the highest, which was 3.5 times of supersonic extraction and 1.5 times of Sohlex extraction and 1.5 times of decocting by water, respectively. The time of MAE was the shortest. MAE could destroy the cell organization to pick up the speed of dissolving. Conclusion : MAE is efficient, saving energy and time in extracting Chinese medicines.
RÉSUMÉ
AIM: To evaluate the characteristic and explore the mechanism of MAE on Semen Cassiae by comparing MAE with commonly used extraction method. METHODS : The amount of anthraquinone was determined by spectrophotometer. The surface and cross section of Semen Cassiae were observed by microphotography. RESULTS : Among the four methods,the efficiency of MAE is 16 times that of ultrasonic extraction,3 times that of Sohlex extraction and 1.1 times of decocting by water,respectively. Micrographs taken after extraction differed markedly indicated that the degree of damage varied considerably. CONCLUSION : The MAE method is more advantageous than other traditional extraction methods (Soxhlet extraction and ultrasonic extraction) with regard to the extraction yield and the time and cost of the procedure.