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1.
China Pharmacy ; (12): 1715-1721, 2024.
Article de Chinois | WPRIM | ID: wpr-1039349

RÉSUMÉ

OBJECTIVE To identify the chemical constituents of Rhamni Songoricae Fructus and to establish their fingerprints and the method for simultaneous determination of four constituents to comprehensively evaluate the quality of Rhamni Songoricae Fructus. METHODS The chemical constituents in Rhamni Songoricae Fructus were qualitatively analyzed by ultra-performance liquid chromatography quadrupole-time-of-flight tandem mass spectrometry. The fingerprints of 15 batches of Rhamni Songoricae Fructus were established by HPLC and chemometric analysis was performed by using SPSS 26.0 and SIMCA 14.1 software; the contents of quercetin, kaempferol, kaempferide and emodin were determined by the same method. RESULTS A total of 35 constituents were identified, including 28 kinds of flavonoids, 5 kinds of anthraquinones and 2 kinds of organic acids. A total of 19 common peaks were identified in the HPLC fingerprints, recognizing quercetin, kaempferol, kaempferide and emodin. The similarities between HPLC fingerprints of 15 batches of samples and control chromatograms were greater than 0.9. The results of cluster analysis showed that 15 batches of samples were divided into 2 classes, of which S1-S5, S7 and S9 were one class and the rest were one class, similar to the results of principal component analysis. The results of the orthogonal partial least squares- discriminant analysis showed that the variable importance projections of peaks No. 2, 6, 1, 11 (quercetin), 3, 14, 8, 10, 19 (emodin), 5 were all greater than 1. The contents of quercetin, kaempferol, kaempferide and emodin ranged from 0.710 to 10.478 mg/g, 0.236 to 0.660 mg/g, 0.334 to 3.039 mg/g, and 0.261 to 0.504 mg/g. CONCLUSIONS The constructed chemical constituent identification, fingerprint and content determination methods are simple, feasible and reproducible, which combined with chemometric analysis can be used for comprehensive evaluation of the quality of Rhamni Songoricae Fructus.

2.
China Pharmacy ; (12): 452-457, 2022.
Article de Chinois | WPRIM | ID: wpr-920462

RÉSUMÉ

OBJECTIVE To establish the fing erprint of Temurin- 5 powder,conduct chemical pattern recognition analysis ,and determine the contents of 4 components simultaneously. METHODS The fingerprints of 10 batches of Temurin- 5 powder were established and similarity evaluation was performed by using high performance liquid chromatography (HPLC)combined with the Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine (2012 edition);common peaks were identified by comparing with mixed substance control. The common peaks were analyzed by systematic cluster analysis and principal component analysis with SPSS 26.0 software. The HPLC method was used to determine the contents of gallic acid , geniposide,chlorogenic acid and ellagic acid in 10 batches of samples. RESULTS A total of 15 common peaks were identified from the fingerprints of 10 batches of Temurin-5 powder,and the similarity was 0.997-0.999. It was identified that peak 1 was gallic acid ,peak 3 was geniposide ,peak 5 was chlorogenic acid and peak 12 was ellagic acid. Among the 10 batches of samples , S4 and S 9 were grouped into one category ,S6-S8 were grouped into one category ,and the other batches of samples were grouped into one category. The accumulative variance contribution rate of first three principal components was 89.245%. The linear ranges of gallic acid ,geniposide,chlorogenic acid and ellagic acid were 5.55-177.5,15.98-511.5,2.56-82.0 and 13.48-431.5 μg/mL, respectively. RSDs of precision ,stability(24 h)and repeatability tests were all less than 2%(n=6 or n=7). The average recoveries were 101.56%,102.21%,98.60% and 96.62%,respectively,RSDs were 1.90%,1.61%,1.58% and 1.73%(n=6). Average contents of above components were 5.03-5.64,10.38-12.16,1.40-1.69,6.47-7.11 mg/g,respectively. CONCLUSIONS The established fingerprint is stable and feasible ,and the content determination method meets the relevant regulations. Combined with chemical pattern recognition analysis ,it can be used for the quality control of Temurin- 5 powder.

3.
Article de Chinois | WPRIM | ID: wpr-663234

RÉSUMÉ

Objective To observe the effect of electroacupuncture on early enteral nutrition (EEN) support in patients with severe craniocerebral injury.Methods A prospective, randomized, controlled study was conducted, 50 patients with severe craniocerebral injury admitted to the the Department of Intensive Care Unit of Traditional Chinese Medicine Hospital of Ningbo from January 2014 to October 2016 were enrolled, and they were randomly divided into electroacupuncture experimental group (26 cases) and conventional treatment control group (24 cases) by randome number table. Enteral nutrition (EN) support was implemented in 24 - 48 hours after admission for all the patients, additionally, the electroacupuncture experimental group was treated by electroacupuncture stimulating acupoint from the 1st day after admission, once 30 minutes daily for consecutive 10 days. The levels of EN calorie reaching standard situation and the parenteral nutrition (PN) necessary for addition were recorded on 3 days and 5 days after admission; the nasal feeding amount of the two groups was recorded on the 1, 4, 7 days; the incidences of complications during EN support period were observed in both groups.Results The proportion of EN calorie reaching the standard in electroacupuncture experimental group was significantly higher than that in the conventional treatment control group in 5 days of treatment [92.3% (24/26) vs. 70.8% (17/24),P < 0.05]. The proportions necessary for addition of PN support were lower in electroacupuncture experimental group than those in the conventional treatment control group on 3 days and 5 days of treatment [3 days: 19.2% (5/26) vs. 25.0% (6/24), 5 days: 7.7% (2/26) vs. 16.7% (4/24)], but there were no significant statistical differences between the two groups (bothP > 0.05). The nasal feeding amounts in electroacupuncture experimental group were significantly higher than those in the conventional treatment control group on 4 days and 7 days of treatment [4 days (mL): 1292.31±123.04 vs.1204.17±139.81,7 days (mL):1342.31±113.75 vs.1275.00±103.21, bothP < 0.05]. The incidence of complications of the electroacupuncture experimental group was significantly lower than that of the conventional treatment control group in 10 days of treatment [26.92% (7/26) vs. 41.67% (10/24),P < 0.05].Conclusions Application of electroacupuncture has a certain clinical value during implementing EN support for treatment of patients with severe craniocerebral injury, early intervention of electroacupuncture can effectively improve the patients' gastrointestinal function and elevate the successful rate of EN, and the therapy is simple, effective and safe without any obvious adverse reactions.

4.
Article de Chinois | WPRIM | ID: wpr-494585

RÉSUMÉ

Objective To observe the clinical efficacy of warm needling in treating chronic prostatitis due to kidney-yang deficiency.Method Seventy-eight patients with chronic prostatitis due to kidney-yang deficiency were randomized into a treatment group of 40 cases and a control group of 38 cases. The treatment group was intervened by warm needling, while the control group was by oral administration of Terazosin hydrochloride tablets. The National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) and white blood cell (WBC) count in prostatic fluid were observed before and after treatment, and the clinical efficacies were compared.Result The total effective rate was 92.5% in the treatment group versus 76.3% in the control group, and the difference was statistically significant (P0.05).Conclusion Warm needling is an effective approach in treating chronic prostatitis due to kidney-yang deficiency.

5.
China Pharmacy ; (12): 2203-2206, 2016.
Article de Chinois | WPRIM | ID: wpr-504492

RÉSUMÉ

OBJECTIVE:To study the bioequivalence of Levodopa micro-capsule floating tablets in Beagle dogs after multi-dose administration. METHODS:6 dogs were collected and divided into Levodopa micro-capsule floating tablets group and Com-pound levodopa preparation group (Benserazide tablet,reference preparation). They were given levodopa 200 mg intragastrically, every 8 h,for consecutive 4 day. In two-period crossover test,HPLC method was established to determine the concentration of le-vodopa in dog. The pharmacokinetic parameter,bioequivalence and plasma concentration fluctuation of steady state were calculated. RESULTS:The main pharmacokinetic parameters of Levodopa micro-capsule floating tablets and reference preparation were as that cmax were(4.23±0.75)and(8.47±1.18)μg/ml;AUC0-∞ were(12.18±1.16)and(13.81±2.12)μg·h/ml;tmax were(1.83±0.26) and(0.67±0.13)h,respectively. 90% confidence intervals for the geometric mean ratio of AUC0-∞ for test and reference prepara-tion were 80.61%-97.90%,and that for cmax were 42.75%-57.63%,respectively. There was statistical significance in tmax between test and reference preparation. Degree of fluctuation of test and reference preparation at steady state were(283.914±43.217)% and (506.489±78.965)%,and fluctuation coefficient were(177.463±7.873)% and(187.405±1.650)%,respectively. The degree of fluctuation of test preparation was significantly less than that of reference preparation. CONCLUSIONS:Levodopa micro-capsule floating tablets show good sustained-release property,and are bioequivalent with reference preparation in absorption after multiple dose administration. It also has lesser fluctuation of blood concentration.

6.
Chinese Pharmacological Bulletin ; (12): 709-715, 2015.
Article de Chinois | WPRIM | ID: wpr-464371

RÉSUMÉ

Aims To study single dose toxicity of poly-phenols effective parts from Punica granatum,to eval-uate their safety,and thus to provide a theoretical basis for drug development and clinical use.To observe their protective effect on ethanol-induced gastric ulcer in rats.Methods 50 healthy Kunming mice were ran-domly divided into five groups and given different doses of polyphenols’effective parts from Punica granatum via intragastric administration.Toxicity and death in each group of mice were observed and recorded after administration for 14 d.The median lethal dose was calculated by Bliss method.70 rats were randomly di-vided into normal group,model group(constant volume of normal saline),sanjiuweitai particles(1 850 mg· kg-1 )group,colloidal bismuth subcitrate (33 mg · kg-1 )group and polyphenols effective parts from Puni-ca granatum low-dose,medium-dose,high-dose(430, 852,1 704 mg·kg-1 )groups.On the 9th day of 10 days’gavage,all except the normal group were fed ethanol (1.5 mL/only)to induce gastric mucosal inju-ry in rats with acute gastric ulcer.Gastric ulcer index, the rate of ulcer inhibition were calculated for each group.The morphological changes of gastric mucosa were observed.The gastric mucosa levels of PGE2 , NO,SOD and MDA were determined.Results The LD50 and 95%confidence limit of the polyphenols’ef-fective parts from Punica granatum were 8 520.9 mg· kg-1 and 7 291.2 ~9 914.4 mg·kg-1,respectively. Pathology showed that the organs receiving dose of 16 000 mg · kg-1 had different degrees of damage . Compared with the model group,the extract from Puni-ca granatum significantly repaired the gastric mucosa, and significantly increased the gastric mucosa levels of NO and reduced MDA content,and improved SOD content and the levels of PGE2 .Conclousion The dose of 5 063 mg · kg-1 of polyphenols effective part from Punica granatum showed no death.The dose of 16 000 mg · kg-1 of polyphenols effective parts from Punica granatum could cause varying degrees of dam-age in heart,liver,lung,kidney or the death of mice. The LD50 and 95% confidence limit of the polyphenols effective parts from Punica granatum were 8 520.9 mg ·kg-1 and 7 291.2 ~9 914.4 mg·kg-1,respective-ly.The extract from Punica granatum plays a protective role against gastric mucosa damage induced by absolute ethanol,and the mechanism may be related to promo-ting ulcer epithelial cells synthesis,enhancing mucosal regeneration function,regulating NO content and en-hancing antioxidant capacity.

7.
China Pharmacist ; (12): 1295-1299, 2015.
Article de Chinois | WPRIM | ID: wpr-670034

RÉSUMÉ

To optimize the formula of levodopa microcapsules floating tablets. Methods:The contents of levodopa and benserazide in the microcapsules floating tablets were determined by HPLC simultaneously. The release rate as the index, an orthogonal design was used to optimize the formula and preparation technology of levodopa microcapsules floating tablets. The release property of the microcapsules floating tablets was evaluated. Results:The HPLC method for the in vitro determination of levodopa and benserazide in the floating tablets met the methodological requirements. The selected formula was as following:the amount ratio of stearic acid, the drugs, acrylic resin and HPMC was 2∶5∶2∶1. The average weight of the tablets was 550 mg. The results of validated tests showed that the microcapsules floating tablets had floating and sustained release property, which could be used by divided dose. Conclusion: The optimized formula of the microcapsules floating tablets is reasonable, and the production process is stable and feasible.

8.
Chinese Journal of Sports Medicine ; (6): 309-312,320, 2010.
Article de Chinois | WPRIM | ID: wpr-598234

RÉSUMÉ

Objective To observe the effect of transcutanous electrical acupoint stimulation(TEAS)on the exercise ability of rats.Methods After stereotaxis surgery,twenty male adult SD rats wera randomly divided into control group (n=10)and TEAS group(n=10).Two days after surgery.the rats were trained to adapt the treadmill mnning and TEAS(continuous wave,2Hz,5mA)was applied to right Zusanli (ST 36) for 30min,once a day for 6 days.Basic microdialysis samples of extracellular serotonin(5-HT)in rats'dorsal raphe nucleus(DRN)were collected 7 days after surgery.During experiment,rats were running on treadmill (10m/min for 10min,then gradually increased to 24m/min for 1 hour,0% grade).Microdialysis samples were collected continuously until 5 hours after treadmill running.The frequency of brush-stimulation during the running was recorded.Meanwhile,plasma amino acid content was examined 5 hours after exercise.Results The frequencies of brush-stimulation during the treadmill running in control group and TEAS group were 2.04+0.46 and 0.40+0.080 times per minute,respectively (P0.05).However,plasma free-TRP(f-TRP)content,f-TRP/T-TRP ratio and f-TRP/BCAA ratio decreased significantly(P<0.05) in TEAS group as compared with the control group.Extracellular 5-HT levels in DRN in control group soon after exercise were higher than their base levels(P<0.05),and decreased in sequential two hours,and then increased significantly 3 hours and 4 hours after exercise as compared with that 2 hours after exercise(P<0.05).There was a slow decline in the levels of extracellular 5-HT in TEAS group at each time-point after exercise,and significant lower than that in control group within 5 hours after exercise(P<0.05).Conclusion TEAS atZusanli(ST36)can effectively improve exercised ability by reducing peripheral free-TRP level and transporting TRP into the brain,and thus decreasing the synthesis of 5-HT in the brain.

9.
Article de Chinois | WPRIM | ID: wpr-560493

RÉSUMÉ

[Objective] To observe the therapeutic effect of electroacupuncture(EA) collagen-induced arthritis(CIA) in rats and explore the partial mechanism via regulating blood rheology.[Methods] Bovine type II collagen was intradermally injected into the back of anesthetized rats to induce arthritis,the animal model of rheumatoid arthritis.Electroacupuncture(continuous waves,2 Hz,5 mA) was applied unilaterally and alternatively to Zusanli(ST 36) and Sanyinjiao(SP 6) for 30min,given once a day for 30 days.The paw edema,pain threshold and joint pathological changes in CIA rats were observed.Some indexes in blood rheology such as viscosity and erythrocyte aggregation were also examined.[Results] EA at point Zuansanli(ST 36) and Sanyinjiao(SP 6) reduced paw swelling and increased pain threshold significantly.Pathological damages of joint in CIA rats were effectively prevented by EA treatment.Meanwhile,the low blood viscosity in EA group were lower obviously than that in control group,and the erythrocyte aggregation indexes were down-regulated by EA.[Conclusion] EA has a pronounced therapeutic effect on collagen-induced arthritis in rats.Regulating the blood rheology in CIA rats may be the mechanism of EA.

10.
Article de Chinois | WPRIM | ID: wpr-681627

RÉSUMÉ

This paper reported all kinds of assay of soybean isoflavone on the basis of collecting and analyzing relating literatures at home and abroad. The assay conditions were listed and compared in this paper. The authors believed that the assay should be selected according to the circumstances.

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