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Adolescent , Humains , Mâle , Femelle , Arrêter de fumer , Fumer , Prévention du fait de fumerRÉSUMÉ
No abstract available.
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Humains , Mâle , Femelle , Hypertension essentielle , Obésité , Tour de taille , Indice de masse corporelle , Maladies cardiovasculairesRÉSUMÉ
No abstract available.
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Coûts hospitaliers , Hôpitaux , Durée du séjour , Hôpitaux à haut volume d'activité , Coûts indirects de la maladie , Assurance maladie , Procédures de chirurgie opératoireRÉSUMÉ
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Haemorrhagic fever with renal syndrome (HFRS) caused by Hantaan viruses has been one of the principal acute febrile disease in Korea. To analysis the sero-epidemiological patterns of HFRS, 4,177 patient sera of acute febrile illness submitted for serological assay to National Institute of Health from Community Health Centers, Institutes of Health and Environment and hospitals from 1996 to 2005 were examined for antibodies against Hantaan virus by indirect immunofluorescent assay (IFA). Serum samples with greater than 1:32 antibody titer were considered positive. The results were analyzed seroepidemiologically by annual, sexual, seasonal, age and regional distribution of HFRS patients. Out of 4,177 serum samples tested, 1,415 samples (33.9%) were positive to Hantaan virus. The ratio of males (48.2%, 682/1,415) to females (38.2%, 541/1,415) was 1.3:1. Seasonal incidence showed that 69.5% (985/1,415) of cases occurred from October to December, resulting with higher prevalence in November (41.3%, 584/1,415). Regionally, seropositive rates of samples collected in Gyenggi, Gangwon and Chungbuk were 39.9% (564/1,415), 19.3% (274/1,415) and 8.5% (120/1,1415), respectively. Age distributions of seropositive of HFRS were detected from 20 to 79 years (78%).
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Femelle , Humains , Mâle , Académies et instituts , Répartition par âge , Anticorps , Centres de santé communautaires , Fièvre , Virus Hantaan , Fièvre hémorragique avec syndrome rénal , Incidence , Corée , Prévalence , SaisonsRÉSUMÉ
To investigate the pattern of drug-resistance of human influenza virus (A/H1N1) isolated in Korea during 2001~2002, the sequence analysis of hemagglutinin (HA) and neuraminidase (NA) genes and cell-based assay against neuraminidase inhibitor (NI) were performed. Analyses on the nucleotide sequences of NA genes showed that Korean isolates had 98.2 to 98.5% homology with that of the vaccine strain in 2001~2002 season, A/New Caledonia/20/99-like strain. However, there were no significant amino acid substitutions related to the drug-resistance such as E119V, R152K, I222R/Q, H274Y, and R292K. In the sequences of HA gene, no differences were observed on the major antigenic sites as well as the motifs related to the drug resistance. 50% inhibitory concentration (IC50) value against oseltamivir, one of NA inhibitors widely used in the treatment for the influenza, was determined by WST-1 assay. The SI values of Korean isolates against oseltamivir were 7.2 to 383.3, showing that these isolates displayed relatively low SI value against the drug. This result provides the useful information for the surveillance of drug-resistant influenza virus and the control of influenza in Korea.
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Substitution d'acide aminé , Séquence nucléotidique , Résistance aux substances , Hémagglutinines , Grippe humaine , Corée , Sialidase , Orthomyxoviridae , Oséltamivir , Saisons , Analyse de séquenceRÉSUMÉ
Japanese encephalitis is a potentially lethal disease of the central nervous system caused by infection with Japanese encephalitis virus (JEV). JEV is the most common cause of encephalitis over a large part of eastern Asia. To establish and characterize in vivo model to study the Japanese encephalitis, the immunohistochemical localization of JEV and the histopathological finding were investigated in the brains of young adult mice infected with JEV by intraperitoneal inoculation. JEV was localized to neurons in discrete regions of the brain. Histopathological finding showed typical pattern of acute viral encephalitis, such as inflammatory cell infiltration in brain parenchyme and perivascular cuffs of mononuclear cells. These results suggest that this in vivo system can be used to study the mechanism of virus entry into the brain, cell specific tropism, and pathophysiology in Japanese encephalitis.
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Animaux , Humains , Souris , Jeune adulte , Asiatiques , Encéphale , Système nerveux central , Encéphalite , Virus de l'encéphalite japonaise (espèce) , Encéphalite japonaise , Encéphalite virale , Extrême-Orient , Immunohistochimie , Neurones , Tropisme , Pénétration viraleRÉSUMÉ
The recent development of molecular diagnostic assays like as polymerase chain reaction (PCR) has provided powerful tools for the diagnosis of viral infection in the clinical fields. To ensure optimal therapeutic and prognostic value, it is important to establish whether viral load measurements are affected by repeated freeze-thaw (FT) cycles since the freezing of clinical samples is a universal method of specimen storage. This study was done to determine the effect of freezing and thawing of various samples on the quantitation and positivity of viral DNA. For this study, three different types of samples being used frequently in clinical fields were selected. Those samples contained ovine herpesvirus-2 (OvHV-2), a member of the gamma herpesviruses (genus Rhadinovirus). Two OvHV-2 DNA positive plasma samples, two peripheral blood mononuclear cell (PBMC) samples, and two nasal swab samples were randomly selected. They were carefully aliquated into 8 tubes for each sample. The aliquoted samples were frozen and thawed 0, 3, 6, 9, 12, 15, 18, and 21 times for each aliquot and then analyzed for changes on DNA levels and positivity. OvHV-2 DNA positivity and quantitation were tested by using nested PCR and real-time PCR, respectively. Twenty-one cycles of freezing and thawing did not significantly change this herpesviral DNA positivity in any of the samples tested. However, the decreases of viral DNA copies were observed in all samples by the increasing of FT cycles. In conclusion, the integrity of herpesviral DNAs in clinical specimens may be degraded by the increasing FT cycles. These results implicate that there is a need to aliquot specimen when it is first collected in order to reduce FT cycles during its analysis.