RÉSUMÉ
By consulting literatures of traditional Chinese medicine(TCM), comparing data about the morphological description of several species from genus of Salvia, and combining the biological studies in resources,this paper made a textual research for the origin,name,medicinal history,functional dominance and folk application Salviae Yunnanensis Radix. This study aimed to provide a reference for further studies and utilization of Salviae Yunnanensis Radix. The findings revealed that the origin of Zidanshen recorded in ancient Chinese herbal literatures was S. yunnanensis,which were widely used for amenorrhea,dysmenorrhea,irregular menstruation,tumescent pain,limb numbness and many kinds of blood stasis. Relevant clinical application and modern pharmacological evidences of Salviae Yunnanensis Radix are mutually supportive,which indicates that TCM based on clinical experience has a high development value. The herbal literatures revealed that Salviae Yunnanensia Radix has a high research and development value in gynecology,blood stasis and other fields. We shall fully excavate folk experience in clinical application of Salviae Yunnanensis Radix. On this basis, we shall further strengthen relevant researches on material basis,pharmacodynamics and mechanism,in order to promote the utilization of resources and product development of Salviae Yunnanensis Radix.
RÉSUMÉ
Fingerprints of lipophilic components in the roots of Salvia miltiorrhiza and S.yunnanensis were analyzed by UPLC-DADand UPLC coupled with mass spectroscopy to evaluate the differences and similarities of the lipophilic components in the two kinds of herbs.The UPLC analysis of 18 batches of S.miltiorrhiza and 16 batches of S.yunnanensis was performed on a 25℃Thermo Accucore C_(18)column(2.1 mm×100 mm,2.6μm)by Shimadzu LC-20AD;mobile phase was 0.026%phosphoric acid(A)-acetonitrile(B)with gradient elution;flow rate was 0.4 m L·min~(-1);detection wavelength was set at 270 nm;injection volume was 2μL.The molecular structures of the lipophilic components were analyzed on a 25℃Thermo Accucore C_(18)column(2.1 mm×100 mm,2.6μm)by Thermo U3000 UPLC Q Exactive Orbitrap LC-MS/MS with a mobile phaseconsisting of 0.1%formic acid water(A)and 0.1%formic acidacetonitrile(B).The mass spectrometry was acquired in positive modes using ESI.There are 10 common peaks in the lipophilic components of S.miltiorrhiza.The similarity between the 16 batches of S.miltiorrhiza and their own reference spectra was greater than 0.942,and the average similarity was 0.973.There are 12 common peaks in the lipophilic components of S.yunnanensis.The similarity between the 18 batches of S.yunnanensis and their own reference spectra was greater than 0.937,and the average similarity was 0.976.The similarity between the reference chromatograms of S.miltiorrhiza and S.yunnanensis was only 0.900.There are three lipophilic components in S.yunnanensis,which are not found in S.miltiorrhiza,and one of which isα-lapachone.There is a lipophilic component in S.miltiorrhiza not found in S.yunnanensis,which may be miltirone.The two herbs contain 8 common lipophilic components including dihydrotanshinoneⅠ,cryptotanshinone,tanshinoneⅠ,tanshinoneⅡ_A,nortanshinone in which the content of tanshinoneⅡ_A,dihydrotanshinoneⅠand cryptotanshinone of S.yunnanensisis significantly lower than that of S.miltiorrhiza(P<0.01),and the contents of tanshinoneⅠand nortanshinone are significantly lower than that of S.miltiorrhiza too(P<0.05).There are significant differences in the types and contents of lipophilic components between the roots of S.miltiorrhiza and S.yunnanensis,and the similarity between the fingerprints of interspecies is much lower than that between the same species.Therefore,the roots of S.miltiorrhiza and S.yunnanensis are two kinds of herbs which are quite different in chemical compounds and compositions.