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1.
Exp. mol. med ; Exp. mol. med;: 341-348, 2009.
Article de Anglais | WPRIM | ID: wpr-136584

RÉSUMÉ

The basic route and mechanism for diapedesis has not yet to be fully defined. Here we present evidence that "cell-cell separation" between endothelial cells (ECs) may provide a route for leukocyte diapedesis. We unexpectedly found that extensive interaction between peripheral blood leukocytes and ECs that were activated by TNF-alpha induced the opening of EC contacts and, surprisingly, resulted in cell-cell separation. This event was specific to the intercellular adhesion molecules-1 (ICAM-1)/leukocyte function-associated antigen-1 interaction, as demonstrated by the following: (1) ICAM-1 expression correlated with increased EC contraction; and (2) the blocking of ICAM-1 selectively inhibited EC separation. Thus, we suggest that "cell-cell separation" could be a mechanism for diapedesis in situations that may require massive leukocyte infiltration.


Sujet(s)
Humains , Mouvement cellulaire , Cellules cultivées , Cellules endothéliales/cytologie , Cytométrie en flux , Technique d'immunofluorescence , Molécule-1 d'adhérence intercellulaire/métabolisme , Leucocytes/cytologie , Antigène-1 associé à la fonction du lymphocyte/métabolisme
2.
Exp. mol. med ; Exp. mol. med;: 341-348, 2009.
Article de Anglais | WPRIM | ID: wpr-136585

RÉSUMÉ

The basic route and mechanism for diapedesis has not yet to be fully defined. Here we present evidence that "cell-cell separation" between endothelial cells (ECs) may provide a route for leukocyte diapedesis. We unexpectedly found that extensive interaction between peripheral blood leukocytes and ECs that were activated by TNF-alpha induced the opening of EC contacts and, surprisingly, resulted in cell-cell separation. This event was specific to the intercellular adhesion molecules-1 (ICAM-1)/leukocyte function-associated antigen-1 interaction, as demonstrated by the following: (1) ICAM-1 expression correlated with increased EC contraction; and (2) the blocking of ICAM-1 selectively inhibited EC separation. Thus, we suggest that "cell-cell separation" could be a mechanism for diapedesis in situations that may require massive leukocyte infiltration.


Sujet(s)
Humains , Mouvement cellulaire , Cellules cultivées , Cellules endothéliales/cytologie , Cytométrie en flux , Technique d'immunofluorescence , Molécule-1 d'adhérence intercellulaire/métabolisme , Leucocytes/cytologie , Antigène-1 associé à la fonction du lymphocyte/métabolisme
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