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2.
Korean J. Ophthalmol ; Korean J. Ophthalmol;: 270-279, 2015.
Article de Anglais | WPRIM | ID: wpr-89398

RÉSUMÉ

PURPOSE: Chronic use of topical hypotensive agents induces several side effects caused by preservatives. The purpose of this study was to evaluate the effects of prostaglandin analogs with varying concentrations of benzalkonium chloride (BAC), preservative-free (PF), and alternative preservatives on mouse corneal tissue. METHODS: Thirty-five, 8- to 10-week-old female C57BL/6 mice (five mice for each group) were used for this study. To the control group, we applied normal saline, and to each drug-treated group we applied 0.02% BAC, bimatoprost 0.01% (with BAC 0.02%), latanoprost 0.005% (with BAC 0.02%), travoprost 0.004% (with 0.001% polyquad) or tafluprost 0.0015% with/without 0.001% BAC, once a day (9 p.m.) for 4 weeks. Corneal fluorescein staining was evaluated in all groups. After harvest, the corneal tissues were embedded in paraffin and then Hematoxylin-Eosin stain was performed for histopathological examination. Immunofluorescence staining was done against TNF-alpha, IL-6, HLA DR, pJNK, and pAkt. RESULTS: In corneal fluorescein staining, severe punctate epithelial keratitis was seen in the groups of 0.02% BAC, 0.02% BAC containing bimatoprost 0.01% and latanoprost 0.005%. The surface desquamation, irregular surface, loss of cell borders, anisocytosis and stromal shrinkage were observed in the groups of BAC-containing eye drops. Moreover, the groups treated with BAC-containing eye drops have high inflammatory markers, significantly decreased cell viability-related signal, pAkt, and higher apoptosis-inducing signal, pJNK, than the control group. On the other hand, travoprost 0.004% and PF tafluprost 0.0015% have less cellular morphologic changes, lower inflammation, and higher cellular viability than BAC-containing formulations. CONCLUSIONS: Corneal damage, increased inflammation and apoptosis and low cell viability were observed in BAC-containing groups. PF or alternatively preserved glaucoma medications seem to be a reasonable and viable alternative to those preserved with BAC.


Sujet(s)
Animaux , Femelle , Souris , Survie cellulaire , Conjonctive/effets des médicaments et des substances chimiques , Modèles animaux de maladie humaine , Épithélium antérieur de la cornée/effets des médicaments et des substances chimiques , Glaucome/traitement médicamenteux , Souris de lignée C57BL , Microscopie de fluorescence , Solutions ophtalmiques , Conservateurs pharmaceutiques , Prostaglandines synthétiques/administration et posologie
3.
Article de Anglais | WPRIM | ID: wpr-176593

RÉSUMÉ

Peroxisome proliferator-activated receptors-gamma (PPAR-gamma) is critical for phenotype determination at early differentiation stages of mesenchymal cells, whereas its physiological role is unclear. Therefore, we investigated the role of 15-deoxy-delta 12,14-prostaglandinJ2 (15d-PGJ2), the natural receptor ligand for PPAR-gamma, on dedifferentiation and inflammatory responses, such as COX-2 expression and PGE2 production, in articular chondrocytes. Our data indicate that the 15d-PGJ2 caused a loss of differentiated chondrocyte phenotype as demonstrated by inhibition of type II collagen and proteoglycan synthesis. 15d-PGJ2 also induced COX-2 expression and PGE2 production. The 15d-PGJ2-induced dedifferentiation effect seems to be dependent on PPAR-gamma activation, as the PPRE luciferase activity increased and PPAR-gamma antagonist, BADGE, abolished type II collagen expression. However, BADGE did not block 15d-PGJ2-induced COX-2 expression. Collectively, our findings suggest that PPAR-gamma-dependent and -independent mechanisms of 15d-PGJ2-induced dedifferentiation and inflammatory responses in articular chondrocytes, respectively. Additionally, these data suggest that targeted modulation of the PPAR-gamma pathway may offer a novel approach for therapeutic inhibition of joint tissue degradation.


Sujet(s)
Animaux , Lapins , Artères/métabolisme , Différenciation cellulaire , Chondrocytes/métabolisme , Cyclooxygenase 2/métabolisme , Dinoprostone/métabolisme , Relation dose-effet des médicaments , Régulation de l'expression des gènes codant pour des enzymes , Gènes rapporteurs , Immunotransfert , Récepteur PPAR gamma/métabolisme , Prostaglandine D2/analogues et dérivés , Facteurs temps , Transfection
4.
Exp. mol. med ; Exp. mol. med;: 53-59, 2003.
Article de Anglais | WPRIM | ID: wpr-78039

RÉSUMÉ

Tumor target-derived soluble secretary factor has been known to influence macrophage activation to induce nitric oxide (NO) production. Since heme oxigenase-1 (HO-1) is induced by a variety of conditions associated with oxidative stress, we questioned whether soluble factor from tumor cells induces HO-1 through NO-dependent mechanism in macrophages. We designated this factor as a tumor-derived macrophage-activating factor (TMAF), because of its ability to activate macrophages to induce iNOS. Although TMAF alone showed modest activity, TMAF in combination with IFN-gamma significantly induced iNOS expression and NO synthesis. Simultaneously, TMAF induced HO-1 and this induction was slightly augmented by IFN-gamma. Surprisingly, however, induction of HO-1 by TMAF was not inhibited by the treatment with the highly selective iNOS inhibitor, 1400 W, indicating that TMAF induces the HO-1 enzyme by a NO-independent mechanism. While rIFN-gamma alone induced iNOS, it had no effect on HO-1 induction by itself. Collectively, the current study reveals that soluble factor from tumor target cells induces HO-1 enzyme in macrophages. However, overall biological significance of this phenomenon remains to be determined.


Sujet(s)
Animaux , Humains , Souris , Antinéoplasiques/pharmacologie , Tumeurs de la vessie urinaire/métabolisme , Lignée cellulaire , Interactions médicamenteuses , Régulation de l'expression des gènes codant pour des enzymes/effets des médicaments et des substances chimiques , Heme oxygenase (decyclizing)/analyse , Interféron gamma/pharmacologie , Activation des macrophages/effets des médicaments et des substances chimiques , Macrophages péritonéaux/métabolisme , Souris de lignée C57BL , Monoxyde d'azote/biosynthèse , Nitric oxide synthase/génétique , Nitrites/analyse , Cellules cancéreuses en culture
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