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ObjectiveTo investigate the mechanism of salvianolic acid F (Sal F) in repairing the high glucose-induced injury in human kidney-2 (HK-2) cells via the B-cell lymphoma-2 (Bcl-2)-associated X protein (Bax)/cysteinyl aspartate-specific proteinase 3 (Caspase-3)/gasdermin-E (GSDME) pathway. MethodThe cell counting kit-8 (CCK-8) was used to measure the relative viability of HK-2 cells exposed to high glucose and different concentrations (2.5, 5, 10, 20 μmol·L-1) of Sal F and the relative viability of HK-2 cells treated with Sal F for different time periods. The levels of lactate dehydrogenase (LDH) and interleukin-1β (IL-1β) in the supernatant of the cell culture were measured by the LDH assay kit and enzyme-linked immunosorbent assay (ELISA) kit, respectively. Flow cytometry combined with Annexin V-FITC/propidium iodide (PI) and Hoechst 33342/PI staining was employed to reveal the proportion of PI-positive HK-2 cells exposed to high glucose. Western blotting was employed to determine the protein levels of Bax, Bcl-2, cytochrome C, cysteinyl aspartate-specific proteinase (Caspase)-9, Caspase-3, and GSDME in the HK-2 cells exposed to high glucose and treated with Sal F. The 2,7-dichlorodihydrofluorescein diacetate fluorescence probe (DCFH-DA) and mitochondrial membrane potential assay kit (JC-1) were used to determine the production of reactive oxygen species (ROS) and the mitochondrial membrane potential in the HK-2 cells exposed to high glucose and treated with Sal F. ResultCompared with the blank group, the model group showed decreased cell viability (P<0.01), elevated levels LDH and IL-1β, increased proportion of PI-positive cells (P<0.01), up-regulated protein levels of Bax, cytochrome C, Caspase-9, Caspase-3, and GSDME (P<0.01), down-regulated protein level of Bcl-2 (P<0.01), decreased mitochondrial membrane potential, and excessive ROS accumulation. Compared with the model group, Sal F repaired the high glucose-induced injury in HK-2 cells (P<0.05), lowered the levels of LDH and IL-1β (P<0.05, P<0.01), and decreased the proportion of PI-positive cells (P<0.01). In addition, Sal F down-regulated the protein levels of Bax, cytochrome C, Caspase-9, Caspase-3, and GSDME and up-regulated the protein level of Bcl-2 (P<0.05, P<0.01), increased the mitochondrial membrane potential, and decreased the accumulation of ROS in HK-2 cells. ConclusionSal F can reduce the production of ROS, restore the balance of mitochondrial membrane potential, and inhibit pyroptosis via the Bax/Caspase-3/GSDME signaling pathway to repair the high glucose-induced injury in HK-2 cells.
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Purpose@#Immunotherapy (IO) plus tyrosine kinase inhibitor (TKI) has become the first-line treatment for advanced renal cell carcinoma, despite the lack of prognostic biomarkers. Cyclin-dependent kinase 5 (CDK5) affects the tumor microenvironment, which may influence the efficacy of TKI+IO. @*Materials and Methods@#Two cohorts from our center (Zhongshan Metastatic Renal Cell Carcinoma [ZS-MRCC] cohort, Zhongshan High-risk Localized Renal Cell Carcinoma [ZS-HRRCC] cohort) and one cohort from a clinical trial (JAVELIN-101) were enrolled. The expression of CDK5 of each sample was determined by RNA sequencing. Immune infiltration and T cell function were evaluated by flow cytometry and immunohistochemistry. Response and progression-free survival (PFS) were set as primary endpoints. @*Results@#Patients of low CDK5 expression showed higher objective response rate (60.0% vs. 23.3%) and longer PFS in both cohorts (ZS-MRCC cohort, p=0.014; JAVELIN-101 cohort, p=0.040). CDK5 expression was enhanced in non-responders (p < 0.05). In the ZS-HRRCC cohort, CDK5 was associated with decreased tumor-infiltrating CD8+ T cells, which was proved by immunohistochemistry (p < 0.05) and flow cytometry (Spearman’s ρ=–0.49, p < 0.001). In the high CDK5 subgroup, CD8+ T cells revealed a dysfunction phenotype with decreased granzyme B, and more regulatory T cells were identified. A predictive score was further constructed by random forest, involving CDK5 and T cell exhaustion features. The RFscore was also validated in both cohorts. By utilizing the model, more patients might be distinguished from the overall cohort. Additionally, only in the low RFscore did TKI+IO outperform TKI monotherapy. @*Conclusion@#High-CDK5 expression was associated with immunosuppression and TKI+IO resistance. RFscore based on CDK5 may be utilized as a biomarker to determine the optimal treatment strategy.
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Fibrosis refers to the final outcome of damage in multiple-type tissue and the imbalance of tissue repair especially in the process of chronic inflammatory response diseases.Fibrosis can occur in various organ tissues.Its continuous progression may lead to organ dysfunction and failure,which is a huge threat to human health.Traditional Chinese medicine has significant therapeutic effects in preventing and treating fibrosis.Due to its characteristics of multiple components,pathways,and targets,it has become a hot research topic in the field of fibrosis.Astragali Radix,a Chinese medicinal for supplementing qi,is the root of Astragalus membranaceus(Fisch.)Bge.var.mongholicus Hisao or Astragalus membranaceus(Fisch.)Bge.It has the effects of replenishing qi and elevating yang,generating fluid and nourishing blood,expelling toxin and draining pus,astringing sore and promoting granulation.It has found that Astragali Radix contains many chemical components such as polysaccharides,saponins,and flavonoids,which have good anti-inflammatory and antioxidant effects.Astragali Radix can effectively intervene in the fibrosis process of multiple organ tissues such as the heart,kidney,liver,and lung.Therefore,this article reviews the anti-fibrotic effects and mechanisms of Astragali Radix and its chemical components,hoping to provide ideas and references for the development and utilization of Astragali Radix.
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ObjectiveTo investigate the possible mechanism of different doses of L-Borneolum,Borneolum,and Borneolum Syntheticum in the electrophysiology,anti-inflammation,and regulation of hypoxia inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) cardiovascular protection of the experimental acute myocardial infarction (AMI) rats. MethodSD male adult rats were randomly divided into thirteen groups according to their body weight,namely the sham operation group,the model group,the solvent model group,the nitroglycerin group,the Borneolum high,medium,and low-dose (0.6,0.3, 0.15 g·kg-1) groups,the L-Borneolum high,medium,and low-dose (0.2,0.1, 0.05 g·kg-1) groups,and the Borneolum Syntheticum high,medium,and low-dose (0.2,0.1, 0.05 g·kg-1) groups,with 10 rats in each group. Rats were given 10 mL·kg-1 by gavage for 3 d of pre-administration. Thirty minutes after the last administration,the left anterior descending coronary artery (LAD) was ligated to induce the model,and the successful rat model was continuously treated for 3 d. BL-420N biosystem was used to analyze the electrocardiogram (ECG) and heart rate variability (HRV) before and after modeling and after 3 d of treatment. Real-time quantitative polymerase chain reaction (Real-time PCR) was used to determine the mRNA expressions of interleukin-1β (IL-1β) and interleukin-6 (IL-6) in the myocardial tissue Western blot and immunohistochemistry were used to determine the protein expression levels of VEGF receptor 1 (VEGFR1),HIF-1α,and CD34. ResultCompared with the sham operation group,the model group significantly increased the heart rate,ECG ST wave,T wave,QRS duration,QTC interval,and Q wave on the day of modeling and after 3 d of treatment,and significantly changed HRV and T wave (P<0.05,P<0.01). As compared with the solvent model group,on the day of modeling,the heart rate of the L-Borneolum medium and low-dose groups and the Borneolum groups,the ST wave of the L-Borneolum groups,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum high-dose group,HRV parameters of the L-Borneolum groups,the Borneolum medium and low-dose groups,and the Borneolum Syntheticum high-dose group,LF/HF of the L-Borneolum high and medium-dose group,the Borneolum low-dose group,and the Borneolum Syntheticum groups,T wave of the L-Borneolum high-dose group,the Borneolum Syntheticum high-dose group,and Borneolum medium-dose group,QTC interval of the L-Borneolum medium and low-dose groups and the Borneolum high and medium-dose groups,and QRS duration of the L-Borneolum high and low-dose groups,the Borneolum high and low-dose groups,and the Borneolum Syntheticum groups were significantly reduced or shortened (P<0.05,P<0.01). After 3 d of treatment,the heart rate of the L-Borneolum groups,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum medium-dose group,ST wave of the L-Borneolum group,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum high-dose group,OTC interval,ORS duration,and Q wave of the L-Borneolum high-dose group,the Borneolum high-dose group,and the Borneolum Syntheticum high and medium-dose groups,QRS duration of the L-Borneolum medium-dose group,QTC interval of the Borneolum medium-dose group,and Q wave of the Borneolum Syntheticum low-dose group were all significantly reduced or shortened(P<0.01). The mRNA expressions of IL-1β and IL-6 in the L-Borneolum medium and low-dose groups,the Borneolum medium and low-dose groups,and the Borneolum Syntheticum high and medium-dose groups were significantly down-regulated(P<0.01),and LF/HF in the L-Borneolum high and medium-dose groups,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum high and low-dose groups were significantly reduced (P<0.05,P<0.01). HRV in the L-Borneolum high-dose group,the Borneolum groups,and the Borneolum Syntheticum high and low-dose groups,and T wave in the Borneolum high and medium-dose groups and the Borneolum Syntheticum high-dose group were increased significantly. The protein expressions of HIF-1α,VEGFR1,and CD34 in the L-Borneolum medium and low-dose groups,the Borneolum low-dose group,and the Borneolum Syntheticum high-dose group were significantly up-regulated,as well as those of VEGFR1 and CD34 in the Borneolum medium-dose group (P<0.05,P<0.01). ConclusionThe 3 kinds of Borneolum improves the heart rate,heart rate variability,and electrocardiogram of AMI model rats to different degrees,and may play a myocardial protective effect by anti-inflammation and promotion of angiogenesis. The combined effect suggests that L-Borneolum has the superior effect next to Borneolum,and Borneolum Syntheticum has the inferior effect.
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ObjectiveTo investigate the possible mechanism of different doses of L-Borneolum,Borneolum,and Borneolum Syntheticum in the electrophysiology,anti-inflammation,and regulation of hypoxia inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) cardiovascular protection of the experimental acute myocardial infarction (AMI) rats. MethodSD male adult rats were randomly divided into thirteen groups according to their body weight,namely the sham operation group,the model group,the solvent model group,the nitroglycerin group,the Borneolum high,medium,and low-dose (0.6,0.3, 0.15 g·kg-1) groups,the L-Borneolum high,medium,and low-dose (0.2,0.1, 0.05 g·kg-1) groups,and the Borneolum Syntheticum high,medium,and low-dose (0.2,0.1, 0.05 g·kg-1) groups,with 10 rats in each group. Rats were given 10 mL·kg-1 by gavage for 3 d of pre-administration. Thirty minutes after the last administration,the left anterior descending coronary artery (LAD) was ligated to induce the model,and the successful rat model was continuously treated for 3 d. BL-420N biosystem was used to analyze the electrocardiogram (ECG) and heart rate variability (HRV) before and after modeling and after 3 d of treatment. Real-time quantitative polymerase chain reaction (Real-time PCR) was used to determine the mRNA expressions of interleukin-1β (IL-1β) and interleukin-6 (IL-6) in the myocardial tissue Western blot and immunohistochemistry were used to determine the protein expression levels of VEGF receptor 1 (VEGFR1),HIF-1α,and CD34. ResultCompared with the sham operation group,the model group significantly increased the heart rate,ECG ST wave,T wave,QRS duration,QTC interval,and Q wave on the day of modeling and after 3 d of treatment,and significantly changed HRV and T wave (P<0.05,P<0.01). As compared with the solvent model group,on the day of modeling,the heart rate of the L-Borneolum medium and low-dose groups and the Borneolum groups,the ST wave of the L-Borneolum groups,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum high-dose group,HRV parameters of the L-Borneolum groups,the Borneolum medium and low-dose groups,and the Borneolum Syntheticum high-dose group,LF/HF of the L-Borneolum high and medium-dose group,the Borneolum low-dose group,and the Borneolum Syntheticum groups,T wave of the L-Borneolum high-dose group,the Borneolum Syntheticum high-dose group,and Borneolum medium-dose group,QTC interval of the L-Borneolum medium and low-dose groups and the Borneolum high and medium-dose groups,and QRS duration of the L-Borneolum high and low-dose groups,the Borneolum high and low-dose groups,and the Borneolum Syntheticum groups were significantly reduced or shortened (P<0.05,P<0.01). After 3 d of treatment,the heart rate of the L-Borneolum groups,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum medium-dose group,ST wave of the L-Borneolum group,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum high-dose group,OTC interval,ORS duration,and Q wave of the L-Borneolum high-dose group,the Borneolum high-dose group,and the Borneolum Syntheticum high and medium-dose groups,QRS duration of the L-Borneolum medium-dose group,QTC interval of the Borneolum medium-dose group,and Q wave of the Borneolum Syntheticum low-dose group were all significantly reduced or shortened(P<0.01). The mRNA expressions of IL-1β and IL-6 in the L-Borneolum medium and low-dose groups,the Borneolum medium and low-dose groups,and the Borneolum Syntheticum high and medium-dose groups were significantly down-regulated(P<0.01),and LF/HF in the L-Borneolum high and medium-dose groups,the Borneolum high and medium-dose groups,and the Borneolum Syntheticum high and low-dose groups were significantly reduced (P<0.05,P<0.01). HRV in the L-Borneolum high-dose group,the Borneolum groups,and the Borneolum Syntheticum high and low-dose groups,and T wave in the Borneolum high and medium-dose groups and the Borneolum Syntheticum high-dose group were increased significantly. The protein expressions of HIF-1α,VEGFR1,and CD34 in the L-Borneolum medium and low-dose groups,the Borneolum low-dose group,and the Borneolum Syntheticum high-dose group were significantly up-regulated,as well as those of VEGFR1 and CD34 in the Borneolum medium-dose group (P<0.05,P<0.01). ConclusionThe 3 kinds of Borneolum improves the heart rate,heart rate variability,and electrocardiogram of AMI model rats to different degrees,and may play a myocardial protective effect by anti-inflammation and promotion of angiogenesis. The combined effect suggests that L-Borneolum has the superior effect next to Borneolum,and Borneolum Syntheticum has the inferior effect.
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ObjectiveTo explore the active components and underlying mechanism of Huanglian Houpotang (HHD) against ulcerative colitis(UC) based on network pharmacology and animal experiments. MethodThe active components of HHD were preliminarily obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine (BATMAN-TCM) and screened out by TCMSP, SwissADME, and SwissTargetPrediction, and their targets were predicted. Relevant microarrays were searched for disease genes with the help of Gene Expression Omnibus (GEO). The common targets of HHD and disease genes were screened out to obtain the potential targets of HHD against UC. The drug-active component-target-disease network was constructed using Cytoscape 3.7.2. The potential therapeutic targets were imported into the DAVID 6.8 for GO-Biological process (GO-BP) analysis to predict related biological processes which were subsequently verified by the animal experiment. Enzyme-linked immunosorbent assay (ELISA) was used to detect the effect of HHD on inflammatory factors in colon tissues of mice. Western blot was used to detect the protein expression of B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and cysteinyl aspartate-specific protease 3 (Caspase-3). The IVIS system was used to detect the content of reactive oxygen species (ROS) in colon tissues of mice in each group. ResultNineteen active components of HHD were screened out, involving 32 potential therapeutic targets against UC and 158 biological processes. The results of the animal experiment showed that HHD exerted its anti-UC effect by inhibiting the expression of inflammatory factors tumor necrosis factor (TNF)-α and interleukin-1β (IL-1β), reducing the content of apoptotic proteins, and regulating the expression of ROS. ConclusionThis study revealed the rationality of predictions and guidance of network pharmacology in experimental design, and confirmed that HHD could exert its effects by participating in biological processes such as immune inflammation, apoptosis, and ROS, which is expected to provide a basis for the mechanism research of HHD in the treatment of UC.
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In recent years, peptide self-assembly has received much attention because of its ability to form regular and ordered structures with diverse functions. Self-assembled peptides can form aggregates with defined structures under specific conditions. They show different characteristics and advantages (e.g., good biocompatibility and high stability) compared with monomeric peptides, which form the basis for potential application in the fields of drug delivery, tissue engineering, and antiseptics. In this paper, the molecular mechanisms, types and influencing factors of forming self-assembled peptides were reviewed, followed by introducing the latest advances on fibrous peptide hydrogels and self-assembled antimicrobial peptides. Furthermore, the challenges and perspectives for peptide self-assembly technology were discussed.
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Systèmes de délivrance de médicaments , Hydrogels , Peptides , Ingénierie tissulaireRésumé
In December 2019, a cluster of patients with pneumonia of unknown cause were linked to a seafood wholesale market in Wuhan, China. Some studies found that the virus was a new kind of virus which had never been found in the human body. Then, the virus was named 2019 novel coronavirus (2019-nCoV) by the World Health Organization (WHO). 2019-nCoV nucleic acid detection is one of the essential indicators of COVID-19. Recently, some false-negative cases in China-Japan Friendship Hospital and Hangzhou Hospital led the clinical doctors to question the value of the nucleic acid detection. In this paper, more than 3 000 results of 2019-nCoV detection in Zhongnan Hospital, Wuhan University were analyzed. Attention should be paid to the root cause of false-negative results and the related countermeasures should be taken.
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In December 2019, a cluster of patients with pneumonia of unknown cause were linked to a seafood wholesale market in Wuhan, China. Some studies found that the virus was a new kind of virus which had never been found in the human body. Then, the virus was named 2019 Novel Coronavirus (2019-nCoV) by the World Health Organization (WHO). 2019-nCoV nucleic acid detection is one of the essential indicators of NCP (Novel Coronavirus Pneumonia). Recently, some false-negative cases in China-Japan Friendship Hospital and Hangzhou Hospital led the clinical doctors to question the value of the nucleic acid detection. In this paper, more than 3 000 results of 2019-nCoV detection in Zhongnan Hospital, Wuhan University were analyzed. Attention should be paid to the root cause of false-negative results and the related countermeasures should be taken.
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Objective To synthesize 18F-AlF-1,4,7-triazacyclononane-l,4,7-triacetic acid (NOTA)-c (CGRRAGGSC),which could specifically bind to the α chain of interleukin (IL)-11 receptor (IL-11 R),and evaluate its targeting potential to IL-11 R-positive tumors.Methods Polypeptide c (CGRRAGGSC) was first coupled with NOTA and then labeled with 18F by AlF labeling method.The radiochemical purity and radiochemical yield of 18F-AlF-NOTA-c(CGRRAGGSC) were analyzed by high performance liquid chromatography,and the stability in vitro was evaluated.The tracer biodistribution in tumor-bearing mice (cell line SKOV3) was evaluated by the dynamic imaging with microPET 30 min,1 h,2 h after injection of 18F-AlF-NOTA-c (CGRRAGGSC).The tracer kinetics was performed in normal mice.Pharmacokinetics parameters were calculated using DAS2.0 software.Results The radiochemical purity of 18F-AlF-NOTA-c(CGRRAGGSC) was higher than 95% and the radiochemical yield was (30.0±7.4)%.It could be stably maintained in phosphatebuffered solution and plasma for at least 2 h.MicroPET imaging showed that 18F-AlF-NOTA-c(CGRRAGGSC)had a good affinity to SKOV3 tumor.The tumor/muscle ratios at 30 min,1 h,2 h after the injection of 18F-AlF-NOTA-c(CGRRAGGSC) were 6.26±2.98,7.19±3.63 and 9.05±4.30,respectively.The tracer was cleared rapidly in blood and mainly excreted by the liver and kidneys.The T1/2α and T1/2β were (0.38±0.14) h and (2.64±0.28) h,respectively.Conclusions 18F-AlF-NOTA-c(CGRRAGGSC) is easy to be synthesized and has a good affinity to IL-11R-positive tumors.It will be a potential IL-11R-targeting imaging agent.
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Objective To discuss the feasibility and clinical outcomes of mini-open reduction through lateral deltoid muscle and locking plate fixation in treatment of proximal humeral fractures.Methods A retrospective study was carried out on 39 patients with proximal humeral fractures treated with mini-open reduction through lateral deltoid muscle and locking plate fixation from September 2012 to September 2015.There were 7 males and 32 females,with a mean age of 66.8 years (range,33-86 years).According to the Neer's classification system,there were 26 patients with two-part fracture and 13 with three-part fracture.visual analogue scale (VAS),Constant-Murley shoulder score,time of bone healing and complications were evaluated for all patients.Results All patients were followed up for 12-48 months (mean,21.3 months).All fractures were proved to be bone healing with duration of (12.0 ± 2.7) weeks.The VAS was improved from preoperative (5.7 ± 1.0) points to (0.9 ± 0.6) points at the final follow-up (P < 0.05).The Constant-Murley shoulder score was increased from preoperative (42.3 ±5.1) points to (89.2± 2.5)points at the final follow-up (P < 0.05).Ranges of shoulder motion were improved with satisfactions.There were no complications like axillary nerve injury,loss of reduction,nonunion or humeral head necrosis.Conclusion For proximal humeral fractures,the miniopen reduction through lateral deltoid muscle and locking plate fixation has advantages of minimal invasion,excellent shoulder function,high rate of bone healing and low incidence of complications.
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Purpose To study of predictive value for detection of high-grade cervical intraepithelial neoplasia (CIN2 +) by p16/Ki-67 dual-stained liquid-based cytology.Methods Random collection of 123 women including 103 samples of atypical squamous cell of undetermined significance (ASC-US) and above with results of high-risk human papillomavirus (HR-HPV)testing and cervical biopsy,20 samples of negative for intraepithelial lesion or malignancy (NILM) by using immunocytochemical p16/Ki-67 dual-stained and the morphology assessment.Results In normal control group,the expression of p16/Ki-67 dual-stained in squamous epithelial cells were negative.Sensitivity of p16/Ki-67 dual-staind cytology for biopsy-confirmed CIN2 + was 66.67% (ASC-US),91.67% (LSIL) and 92.86% (HSIL),specificity rates were 95.92% (ASC-US),95.00% (LSIL) and 0 (HSIL),positive predictive value were 50.00% (ASC-US),91.67% (LSIL) and 92.86% (HSIL),negative predictive value were 97.92% (ASC-US),95.00%(LSIL) and 0 (HSIL),respectively.Condusion p16/Ki-67 dual-stained cytology are improved obviously the predictive value for detection of CIN2 +,p16/Ki-67 dual-stained cytology may efficiently complement HPV-based screening programs to prevent cervical cancer.
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Objective To explore the therapeutic efficacy of percutaneous compression plate internal fixation in the treatment for elder-ly patients with interchanteric fractures. Methods All 17 patients with interchanteric fractures from March 2012 to January 2013 were trea-ted with percutaneous compression plate internal fixation(Fracture classification:type A1. 2 with 3cases,A1. 3 with 4cases,A2. 1 with 6 ca-ses,A2. 2 with 3 cases,A2. 3 with 1 case). After operation,the patients did functional exercises at the early stage. Then the efficacy were e-valuated and analyzed. Results Operation time was 30~80 min,with average time 50 min, blood loss was (40~100) mL,with average volume 60 mL,time of X-ray exposure was 12~22 s,with average time 20 s. All 17 patients were followed up for 3~18 months. Postoperative function were evaluated according to Harris Hip Score,15 cases were excellent,and 2 cases were good,the excellent and good rate was 100%. Conclusion Percutaneous compression plate internal fixation is suitable for the elderly patients of interchanteric fractures,with shorter oper-ation time,less blood loss,reliable fixation and less complications.
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Coronary artery lesions caused by Kawasaki disease have main effect to its prognosis.Thelesions can be showed as arteriitis,arteriectasis,aneurysm and arterial stenosis stenosis.Ultrasonic cardiography is the first choice to detect the lesions,especially in dilatation and aneurysm formation of the coronary artery.It can definitely show the diameter and the degree of dilatation of the coronary artery.With the development of ultrasonic equipments as well as the applications of new technique,ultrasonic cardiography is playing an important role in Kawasaki disease of early diagnosis,therapeutic effect,prognosis estimation and Iongterm follow -up.
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ObjectiveTo compare the efficacy and tolerability of 1-week 1% terbinafine hydrochloride cream, 1- and 4-week 2% miconazole nitrate cream in the treatment of interdigital tinea pedis, and to observe the relapse in patients treated with these regimens. MethodsA multi-center, randomized, double-blind and parallel group study was conducted. By using a stratified randomization protocol, patients were divided into 3 groups to apply terbinafine cream twice daily for 1 week and inert cream(placebo) for the next 3 weeks (1week terbinafine group), miconazole cream twice daily for 1 week and inert cream(placebo) for the next 3 weeks (1-week miconazole group), and miconazole cream twice daily for 4 weeks (4-week miconazole group),respectively. Clinical and mycological assessment was made on week 1, 3, 4, 6, 9 and 12 after the initiation of treatment. ResultsA total of 152 patients with positive baseline mycological culture were eligible for the efficacy analysis. After 4-week treatment, the mycological cure rates were 94.7%, 87.8% and 82.6%, global effective rates 89.5%, 81.6% and 63.0%, respectively for the 1-week terbinafine group, 4-week miconazole group and 1-week miconazole group. On week 12, the mycological relapse rates in 1-week terbinafine, 4-week miconazole and 1-week miconazole group were 13%, 14% and 21% respectively, and the incidence of adverse reaction was 2.38%, 2.38% and 3.57%, respectively. ConclusionsAs far as the efficacy and recurrence in patients are concerned, the 1-week terbinafine cream regimen is similar to the 4-week miconazole cream regimen for the treatment of interdigital tinea pedis.
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The birth rate of very low birth weight(VLBW)is rising year by year,and its survival rate increases with developing of modern methods of neonatal intensive care.The outcomes of VLBW have become a social concern.VLBW have different brain structure compared with term children,mainly in the reduction of brain volume.including the reduction of gray matter volume and white matter vollame.Because of these pathologic changes in brain.some VLBW show severe neurodevelopmental disorders in childhood,including cognitive problems.behavioral and psychological problems,motor clumsiness and so on.
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72 cases of treatments on insomnia were recorded in the medical books of Ming and Qing dynasties(A.D.1368~1911 years).The compatibility regularity of treating insomnia in these 72 cases were statistically analyzed with frequency and the R type cluster analysis methods. Combining the data and modern recognition, compatibility and medication regularity of treating insomnia was further analyzed.
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A new 2450MHz invasive antenna for microwave hyperthermia is designed in this paper. The finite element method is employed to simulate the choke antenna and improved choke antenna, i.e., choke-umbrella antenna. The specific absorption rates (SAR) in tumors are calculated with the two kinds of antennas. S11 of choke-umbrella antenna is also compared with that of choke antenna. Multi-layered block of tissue-equivalent phantom is heated with the fabricated invasive antenna. The distribution of the temperature field in the invasive layer as well as the three-dimensional distribution of the temperature field in phantom is obtained with an infrared thermo graphic technique. Research results show that the improved choke-umbrella antenna can generate more uniform temperature distribution and meets the requirements of the actual clinical microwave hyperthermia better.
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Conception d'appareillage , Hyperthermie provoquée , Micro-ondesRésumé
0.05), the same results were seen in the interferential experiment groups and the positive group. There were no alternating effects between nano red elemental selenium and CCl4. Conclusion In the present paper, nano red elemental selenium does not show an adverse effect on the liver of rats and a protective effect on liver injury induced by CCl4.
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Objective To study the distribution of genotypes of Candida albicans isolated from different body sites of patients with candidal vulvovaginitis(CVV).Methods PCR was designed to amplify group I intron-containing region in25S rDNA of Candida albicans.The strains of Candida albicans could be classified into three genotypes:genotype A(~450bp),B(~840bp)and C(~450bp and~840bp),on the basis of different ranges of bands of amplicons.Results Sixty women with CVV were recruited,of whom54were caused by Candida albicans.Among the54patients39had non-recurrent CVV and15had recurrent CVV(RCVV).Candida albicans could be isolated simultaneously from different body sites in32of54patients,including19(19/39)with non-RCVV and13(13/15)with RCVV.A total of92strains of Candida albicans were isolated from vagina,tongue and anus in54patients with CVV.Eighty strains of genotype A,8of genotype B and4of genotype C were found.The same genotypes of Candida albicans in different body sites were identified in24patients,and the different genotypes were identified in8patients.Conclusion Genotype A is predominant in CVV.The other two genotypes(B and C)are not commonly seen,and mainly isolated from non-vaginal sites.The colonization of Candida albicans in the non-vagina sites is more frequent in RCVV than that in CVV,and the intestinal reservoir theory may play a role in the relapse of RCVV.