Isolation and quantification of a 17-epi-dammarane triterpenoid rhuslactone from roots of Rhus chinensis and its preventive effects on coronary heart disease and thrombosis in zebrafish / 中国中药杂志

Based on mass spectrometry(MS)-guided separation strategy, compound 1 was obtained from the roots of Rhus chinensis. By comprehensive analysis of high resolution-electrospray ionization-mass spectrometry(HR-ESI-MS), nuclear magnetic resonance(NMR) data, and quantum chemical calculation of NMR(qcc-NMR) parameters, compound 1 was elucidated as rhuslactone, a 17-epi-dammarane triterpenoid with a rare 17α-side chain. An HPLC-ELSD method for its quantification in R. chinensis was established and adopted for the quantification of rhuslactone in different batches of R. chinensis. Rhuslactone displayed a good linear relationship within the range of 0.021 3-1.07 μmol·mL~(-1 )(r=0.997 6), and the average recovery was 99.34% [relative standard deviation(RSD) 2.9%). Moreover, the results of the evaluation test of the preventive effects of rhusalctone on coronary heart disease(CHD) and thrombosis showed that rhuslactone(0.11 nmol·mL~(-1)) significantly alleviated heart enlargement and venous congestion and increased cardiac output(CO), blood flow velocity(BFV), and heart rate, thereby reducing thrombus formation in zebrafish with CHD. The effects of rhuslactone on CO and BFV were superior to that of digoxin(1.02 nmol·mL~(-1)), and its effect on improving heart rate was comparable to that of digoxin. This study provides experimental references for the isolation, identification, quality control, and application of rhuslactone from R. chinensis against CHD. It is worth mentioning that this study has discussed some omissions in the determination of the stereochemistry of C-17 in dammarane triterpenoids in the present coursebook Chemistry of Chinese Medicine and some research papers, that is, the compound may be 17-epi-dammarane triterpenoid. This paper has also proposed steps for the establishment of C-17 stereochemistry.

Comparison of different protocols for protein extraction from formalin-fixed paraffin embedded esophageal squamous cell carcinoma tissues / 解剖学报

Objective To explore protein extraction efficiency from formaldehyde-fixed paraffin embedded (FFPE) esophageal squamous cell carcinoma (ESCC) tissue samples with different protocols. Methods Six different lysis buffers with 100 °C or 105 °C. treatments were used for protein extraction, followed by evaluation of protein quantity and quality with Bradford, sodium dodecyl sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis, Western blotting and immunohistochemistry (IHC), using 8 FFPE samples of ESCC. Results The optimal method for protein extraction from FFPE ESCC tissue was Laemmli lysis buffer (Buffer 4) treated with 100 °C incubation, evidenced by highest amount of protein recovery. Western blotting and IHC method measured consistent 14-3-3σ expression in FFPE ESCC tissue samples. Protein precipitated by two volumes of acetonitrite acetonitrile(ACN) (0.1% trifluoroacetic acid) relative to protein amount reduced background staining on SDS-PAGE gels by commassie staining. Conclusion Laemmli lysis buffer combined with 100 °C incubation has the highest protein extraction efficiency from FFPE ESCC tissue samples for Western blotting measurement of protein biomarkers, and ACN protein precipitation can further eliminate residual cross- linked protein by FFPE.

Verification on the Developmental Toxicity of Short-term Exposure to Phenol in Rats / 生物医学与环境科学（英文）

Objective@#To verify the health advisory for short-term exposure to phenol.@*Methods@#The method of this validation experiment was the same as the US Environmental Protection Agency (EPA) methodology for toxicology experiments used to determine phenol drinking water equivalent level (DWEL). Pregnant female Sprague-Dawley rats were administered phenol in distilled water by gavage at daily doses of 15, 30, 60, 120, and 240 mg/kg body weight (b.w.) from implantation (the 6th day post-mating) to the day prior to the scheduled caesarean section (the 20th day of pregnancy). The following information was recorded: general behavior; body weight; number of corpus luteum, live birth, fetus, stillbirth, and implantation; fetal gender; body weight; body length; tail length; and abnormalities and pathomorphological changes in the dams.@*Results@#In the 60 mg/kg b.w. dose group, the mortality of pregnant rats increased with increasing doses, suggesting maternal toxicity. Fetal and placental weights decreased as phenol dose increased from 30 mg/kg b.w., and were significantly different compared those in the vehicle control group, which suggested developmental toxicity in the fetuses. However, the phenol-exposed groups showed no significant change in other parameters compared with the vehicle control group ( > 0.05).@*Conclusion@#Despite using the same method as the US EPA, a different NOEAL of 15 mg/(kg·d) was obtained in this study.

Effect of 3D laparoscopic hepatectomy in glissonian approach for hepatocellular carcinoma on the cellular immune function / 实用医学杂志

Objective To compare the effects of 3D laparoscopic hepatectomy (3D-LH) and open hepatectomy(OH)in glissonian access for hepatocellular carcinoma(HCC)on postoperative immunosuppression by analyzing cellular immune function. Methods During January 2014 to December 2017,sixty patients randomly scheduled to undergo hepatectomy were divided into 3D-LH group and OH group,with 30 patients in each group. All patients' peripheral blood was sampled to measure cell-mediated-immunologic markers(CD4+,CD8+T cell) before operation and in the mornings of the 1st,7thday after surgery. The effects of different operative methods on cellular immune function in patients organism were investigated. Results The decreased level in 3D-LH group was less than that in OH group in postoperative 1stday. The improvement in the 3D-LH group was more faster than that in the OH group on postoperative 7thday. There was statistical significance between the two groups(P<0.05), respectively. Conclusion When compared to open hepatectomy ,3D laparoscopy liver resection in glissonian approach for HCC,less effects in cellular immune function.

Comparison of 5-year outcomes of zotarolimus-eluting stents and everolimus-eluting stents / 中国介入心脏病学杂志

Objective To compare 5-year clinical outcomes of the safety and efficacy of zotarolimus-eluting stent(ZES)and everolimus-eluting stent(EES).Methods A total of 480 patients receiving either ZES(n=244)or EES(n=236)placement from January 2011 to December 2012 in Wuhan Asia Heart Hospital were included in this study.The primary end point of the study was 5-year all-cause mortality including acute myocardial infarction,and target vessel revascularization after the index EES/ZES placement.The secondary end points included subsequent in-stent restenosis and in-stent occlusion at 5 years.Results There were no significant differences in all-cause mortality and acute myocardial infarction outcomes between the ZES and the EES groups(P＞0.05).Compared with patients receiving EES,patients receiving ZES had a significantly higher rate of 5-year target vessel revascularization(10.2％vs.5.1％,P=0.040),in-stent restenosis(11.5％vs.5.9％,P=0.036),and in-stent occlusion(6.1％vs.2.1％,P=0.038).Conclusions At 5 years,patients receiving EES generally had better or comparable long-term outcomes compared with patients receiving ZES.

Comparison of the diagnostic value of routine electrocardiogram and dynamic electrocardiogram in the diagnosis of arrhythmia and heart rate variability in children / 中国基层医药

Objective To compare the application value of routine electrocardiogram and dynamic electrocardiogram in the diagnosis of arrhythmia and heart rate variability in children.Methods In our hospital,pediatric arrhythmia patients were given routine electrocardiogram and dynamic electrocardiogram.The relationship between arrhythmia and heart rate variability in children was analyzed.Results Supraventricular tachycardia (13.33%),ventricular tachycardia(5.56%),premature ventricular contraction (21.11%),premature atrial contraction (14.44%),block (12.22%) in the dynamic electrocardiogram were significantly better than the conventional electrocardiogram,and the differences were statistically significant x2=11.93,4.56,9.22,10.74,11.55,all P<0.05).There were significant differences in the heart rate variability between ventricular arrhythmias and non-ventricular arrhythmias in children[(78.85±0.88),(4.68±2.43),(24.78±2.56),(21.66±0.94), t=10.88,11.79,9.84,9.92,all P<0.05].Conclusion Compared with routine electrocardiogram, dynamic electrocardiogram has better diagnostic value for pediatric arrhythmia of ventricular tachycardia.The detection rate of ventricular tachycardia,ventricular tachycardia,premature ventricular contraction,atrial premature contraction,conduction block and other arrhythmias are obviously superior.And dynamic ECG measurement of heart rate variability is helpful to assess the severity of the disease.

Analysis of growth plate material property effect on knee injury of six-year-old child occupant / 医用生物力学

Objective To develop the finite element model of six-year-old child occupant lower extremity with higher biofidelity and validate the model of knee joints, as well as analyze the biomechanical responses of growth plate under frontal impact load and injury mechanisms of the knee joint by using this model. Methods The six-year-old child occupant lower extremity with growth plate was modeled based on children’s anatomy and CT images, and corresponding material properties of the lower extremity model were assigned．The model was validated according to biomechanical experiments by Kerrigan et al. and Haut et al. and then was used to analyze the injury results of growth plate with different material properties. Results The model validation was qualified by comparing the curves from the experimental and simulation results．The growth plates at knee regions could change injury patterns of the child occupant lower extremity fracture. The material properties of growth plate could affect threshold of axial damage of the femur as well as relative position of the fracture． Conclusions The validated model can be used for related study and application on biomechanical responses and injury mechanisms of six-year-old child occupant lower extremities．

Development and validation for finite element model of one-year-old toddler head with detailed anatomical structures / 医用生物力学

Objective To explore the brain injury mechanism and enrich the database of human finite element (FE) biomechanical model by developing the FE model of one-year-old toddler head. Methods Based on CT data from Chinese one-year-old toddler head with substantial and detailed information, the head model with detailed anatomical structure was constructed by using the medical software Mimics to get the head geometry data, as well as the reverse engineering software to divide NURBS surface and build the geometric model. Finally, the FE pre-processing software was used mesh the model. The FE model of one-year-old toddle head was validated by data from anatomic and cadaver experiments, and was used for preliminary analysis on damage mechanism of one-year-old toddler head. Results The FE model of Chinese one-year-old male toddler head was developed, which included and distinguished the gray matter and white matter of brain and cerebellum, hippocampus, fontanel, sagittal suture, coronal suture, brainstem and ventricles. The cadaver head static compression experiments and drop experiments were reconstructed by using this head model, and the results showed that the FE model of head had similar mechanical properties with the cadaver, which proved the validity of the FE model. Simulation results showed that skull stiffness and skull injury severity varied with different compression rates. Conclusions The FE model of one-year-old toddler head with detailed anatomical structures is of great biofidelity. The FE head model can be used to further investigate the detailed injury mechanism of deep brain tissues, especially for the closed craniocerebral injury, which provides an effective way and tool for the related research and clinical application.

Analysis of growth plate material property effect on knee injury of six-year-old child occupant / 医用生物力学

Objective To develop the finite element model of six-year-old child occupant lower extremity with higher biofidelity and validate the model of knee joints,as well as analyze the biomechanical responses of growth plate under frontal impact load and injury mechanisms of the knee joint by using this model.Methods The sixyear-old child occupant lower extremity with growth plate was modeled based on children's anatomy and CT images,and corresponding material properties of the lower extremity model were assigned.The model was validated according to biomechanical experiments by Kerrigan et aL and Haut et aL and then was used to analyze the injury results of growth plate with different material properties.Results The model validation was qualified by comparing the curves from the experimental and simulation results.The growth plates at knee regions could change injury patterns of the child occupant lower extremity fracture.The material properties of growth plate could affect threshold of axial damage of the femur as well as relative position of the fracture.Conclusions The validated model can be used for related study and application on biomechanical responses and injury mechanisms of sixyear-old child occupant lower extremities.

Analysis of growth plate material property effect on knee injury of six-year-old child occupant / 医用生物力学

Objective To develop the finite element model of six-year-old child occupant lower extremity with higher biofidelity and validate the model of knee joints,as well as analyze the biomechanical responses of growth plate under frontal impact load and injury mechanisms of the knee joint by using this model.Methods The sixyear-old child occupant lower extremity with growth plate was modeled based on children's anatomy and CT images,and corresponding material properties of the lower extremity model were assigned.The model was validated according to biomechanical experiments by Kerrigan et aL and Haut et aL and then was used to analyze the injury results of growth plate with different material properties.Results The model validation was qualified by comparing the curves from the experimental and simulation results.The growth plates at knee regions could change injury patterns of the child occupant lower extremity fracture.The material properties of growth plate could affect threshold of axial damage of the femur as well as relative position of the fracture.Conclusions The validated model can be used for related study and application on biomechanical responses and injury mechanisms of sixyear-old child occupant lower extremities.

Influence of intratracheal instillation of curosurf on right ventricular function in neonatal respiratory distress syndrome / 中国生化药物杂志

Objective To investigate the effect of intratracheal instillation of curosurf on neonatal respiratory distress syndrome ( NRDS ) in children with right ventricular function.Methods 52 patients with NRDS were retrospectively selected and divided into two groups according to different treatment.The patients in the conventional group were treated with nasal airway ventilation.Based on the conventional group, the curosurf group was taken with curosurf.26 cases were in each group.The blood gas index (PaCO2, PaO2, pH), inflammatory reaction (TNF-α, IL-10), SF of the two groups were compared, the complications and curative effect of the two groups before and after treatment were taken for statistics.Results There was no significant difference in pH value between the two groups at each time point.The PaO2 expression levels in the curosurf group at different time points after treatment were significantly lower than the conventional group (P<0.05).The levels of TNF-αand IL-10 in the curosurf group were more stable than those in the control group at different time points after treatment (P<0.05), and the degree of SF increasing at different time points were higher (P<0.05).The total effective rate 80.77% of the curosurf group was significantly higher than that of the conventional group 61.54%(P<0.05).The total complication rate 19.24% had no significant differences with the conventional group 23.08%.Conclusion Intratracheal instillation of CsA in the treatment of NRDS has the advantages of simple operation, little side effect, rapid recovery of blood gas index and inflammatory factors, so it is a feasible method for clinical treatment of NRDS.

Development and validation of finite element model for the 6-year-old pediatric neck / 医用生物力学

Objective To predict biomechanical responses of neck injuries under different loading conditions based on the finite element model of the 6-year-old pediatric neck. Methods The finite element model of the 6-year-old pediatric neck with real anatomical structural muscles was developed, according to the CT images. The model was verified by reconstructing the dynamic tensile test of different cervical spine segments, the tensile test of full cervical spine and the low speed impact experiment of the pediatric volunteers. Results The force-displacement curves, obtained from the simulations on tensile test of different cervical spine segments and tensile test of full cervical spine, were in good agreement with the experimental curves. The head angular velocity-time curve obtained from simulations on pediatric volunteer was consistent with the corridor obtained from experimental data. Conclusions The model is validated and can be used for studying the biomechanical responses and injury mechanism of pediatric neck under different loading conditions.

Effects from material parameters of craniocerebral tissues on impact responses of child head / 医用生物力学

Objective In view of the uncertainties of material parameters for child craniocerebral tissues, the effects of such parameters on responses of child head under direct impact loads were investigated. Methods The impact simulation experiments were conducted under direct impact loads by using a validated finite element model of 3-year-old child head. Taguchi orthogonal method and variance analysis were performed to analyze the material properties of craniocerebral tissues in child head. Results Elastic modulus of the skull had statistically significant effects on impact responses of child head. With the skull elastic modulus increasing, the coup pressure decreased significantly (P=0.000), whereas the contrecoup pressure (P=0.000) and maximum Von Mises stress of the skull increased significantly (P=0.000). The linear viscoelastic material parameters of brain tissues also had statistically significant effects on impact responses of child head. With the increase of the short-time shear modulus of brain tissues, the maximum shear stress of brain tissues increased significantly (P=0.000) whereas the maximum principal strain decreased significantly (P=0.000). Conclusions These statistical analysis results can provide references for selecting material parameter of craniocerebral tissues in finite element model of child head, which will be helpful to improve diagnosis accuracy of brain injuries such as concussion difficult to be definitely diagnosed when using brain CT images in clinic.

Construction of CD19-CAR retroviral vector and modification of its transduction of human T-lymphocytes / 中华血液学杂志

<p><b>OBJECTIVE</b>To improve the MigR1-CD19-CAR (chimeric antigen receptor) that contains a single chain variable region (scFv) which targeted to CD19 through a retroviral vector transduction efficiency of T-lymphocytes.</p><p><b>METHODS</b>Insert the CD19-CAR fragment into the retroviral vector (MigR1) through recombinant DNA technology, after transfecting plat-A packaging cell lines, viral supernatant was collected to transduce K562 cell line and activated human T-lymphocytes. We used flow cytometry to determine the transduction efficiency and RT-PCR to confirm the transcription of CD19-CAR gene. The ability of the transduced T cells to produce IFN-γ and TNF-α in a CD19-specific manner was measured in an enzyme-linked immunosorbent (ELISA) assay.</p><p><b>RESULTS</b>(1)Using MigR1-CD19-CAR retroviral vector to produce the high titer retrovirus. (2)MigR1-CD19-CAR transduction efficiency of K562 cell line was significantly higher than human T-lymphocytes (P<0.01). (3)120 min centrifugation could significantly improve transduction efficiency of T-lymphocytes to (54.5±14.6)%. (4)Transduction efficiency could be improved by deciding transduce time according to T-lymphocytes proliferation fold in vitro individually, and the highest transduction efficiency in the study was 69.3%. The CD19-CAR gene sequence was transcripted specificly with high efficiency. (5) IFN-γ and TNF-α released by CD19-CAR transduced T-lymphocytes significantly increased to (13 230±1 543) pg/ml and (4 217±211) pg/ml when coculture with CD19-K562 cells.</p><p><b>CONCLUSION</b>We have successfully constructed a second generation CAR which targeted to CD19 through a retroviral vector called MigR1 (MigR1-CD19-CAR). Deciding transduce time according to T-lymphocytes proliferation fold in vitro individually and 120 min centrifugation could improve the CAR transduction efficiency of T-lymphocytes. RT-PCR confirmed that the CD19-CAR gene was specificly transcripted with high efficiency. IFN-γ and TNF-α released by CD19-CAR transduced T-lymphocytes significantly increased when activated by target cells.</p>

Impact of libido at 2 weeks after stroke on risk of stroke recurrence at 1-year in a chinese stroke cohort study / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>There were few studies on the relation between changes in libido and incidence of stroke recurrence. The aim of this study was to investigate the relationship between libido decrease at 2 weeks after stroke and recurrent stroke at 1-year.</p><p><b>METHODS</b>It is a multi-centered, prospective cohort study. The 14 th item of the Hamilton Depression Rating Scale-17 was used to evaluate changes of libido in poststroke patients at 2 weeks. Stroke recurrence was defined as an aggravation of former neurological functional deficit, new local or overall symptoms, or stroke diagnosed at re-admission.</p><p><b>RESULTS</b>Among 2341 enrolled patients, 1757 patients had completed follow-up data, 533 (30.34%) patients had decreased libido at 2 weeks, and 166 (9.45%) patients had recurrent stroke at 1-year. Multivariate logistic regression analysis showed that, compared with patients with normal libido, the odds ratio (OR) of recurrent stroke in patients with decreased libido was reduced by 41% (OR = 0.59, 95% confidence interval [CI]: 0.40-0.87). The correlation was more prominent among male patients (OR = 0.52, 95% CI: 0.31-0.85) and patients of ≥60 years of age (OR = 0.57, 95% CI: 0.35-0.93).</p><p><b>CONCLUSIONS</b>One out of three stroke patients in mainland China has decreased libido at 2 weeks after stroke. Decreased libido is a protective factor for stroke recurrence at 1-year, which is more prominent among older male patients.</p>

Development and validation for the finite element model of child head / 医用生物力学

Objective To investigate brain responses from children during traffic accident and drop impact by developing a 3-year-old child head finite element (FE) model. Methods Based on the CT data from a 4-year-old child head, the FE child head model with detaile

Establishment of the CD19-K562 cell line over-expressing stably CD19 gene and its subcutaneous xenograft model in NOD-SCID mouse / 白血病·淋巴瘤

Objective To construct a MigR1-CD19 recombinant vector which contains CD19 gene, and to establish a CD19-K562 cell line over-expressing stably CD19 gene and its subcutaneous xenograft model in NOD-SCID mouse.Methods The CD19 gene was inserted into the retroviral vector (MigR1) through recombinant DNA technology after transfection into Plat-A packaging cells, and viral supernatant was collected to transduce K562 cell line repeatedly to obtain stable transduction CD19-K562 cell line.Flow cytometry was used to determine the transduction efficiency and RT-PCR was used to confirmed CD19 gene expression.Cell proliferation and apoptosis were detected by cell count and Annexin V/PI, respectively.Then the subcutaneous xenograft subtype of CD19-K562-a cell line was constructed through subcutaneous inoculation and was cultured in vitro and in vivo.Then its subcutaneous xenograft model in NOD-SCID mouse was established.The characteristics of CD19-K562-a cells were detected by RT-PCR, Wright staining and immunohistochemistry.Results MigR1-CD19 recombinant vector was successfully constructed, and the CD19 positive efficiency of K562 cell line was (99.80±0.17) ％ through retrovirus centrifugation transduction.The transduction and passage had no effects on proliferation and apoptosis of CD19-K562 cells.The CD19-K562-a cell line was constructed after CD19-K562 cells were injected subcutaneously and were passaged in vitro and in vivo.The CD19 positive efficiency of the xenograft subtype CD19-K562-a cell line was (99.78± 0.04) ％.CD19-K562-a and CD19-K562 cells were in an undifferentiated state.NOD-SCID subcutaneous xenografts were established through subcutaneous inoculation of CD19-K562-a cells.CD19 in the CD19-K562-a subcutaneous xenografts was positive, while it was negative in its counterparts K562 cells.Conclusion The CD19-K562 cell line over-expressing CD19 gene and its subcutaneous xenograft model in NOD-SCID mouse are successfully established.

Analysis on constitutive models and biomechanical properties of brain / 医用生物力学

Brain injury has become the most severe injury in traffic accident due to its high incidence and high fatality rate. The brain injury model plays a critical role in researches on brain injury. The constitutive model and the material properties used in the model are key factors in determining the accuracy of injury prediction. The present paper aims to review the application of constitutive models and material properties in brain simulation so as to better understand biomechanical properties of brain and provide references for finite element researches on brain injury.

Skeleton and soft tissue contour reconstruction for severe progressive hemifacial atrophy / 中华整形外科杂志

<p><b>OBJECTIVE</b>To sum up the various procedures for skeleton and soft tissue contour reconstruction in severe progressive hemifacial atrophy.</p><p><b>METHODS</b>From Jan 2004 to May 2012, 25 patients with severe progressive hemifacial atrophy underwent the procedures of lipoinjection, microsurgical flap transplantation, dermis grafting, distraction osteogenesis, orthognathic surgery and so on for both skeleton and soft tissue reconstruction.</p><p><b>RESULTS</b>Among them, zygomatic augmentation and lipoinjection were performed in 24 cases, anterolateral thigh adipofascial flap in 10 cases and latissimus dorsi flap in one case, orthognathic surgery in 17 cases, including Le Fort I osetoectomy in 3 cases, genioplasty in 4 cases, mandibular distraction osteogenesis combined with secondary Le Fort I osteotomy in 3 cases, genioplasty combined with mandibular augmentation with Medpor implant in 7 cases. The patients were followed up for 6 months to 5 years. Through skelton and soft tissue reconstruction, the oblique occlusion plane and malocclusion were corrected with great improvement in face asymmetry.</p><p><b>CONCLUSIONS</b>For severe progreassive hemifacial atrophy, comprehensive procedures should be adopted for both skelton and soft tissue reconstruction to achieve good results.</p>

Finite element evaluation on skull-brain relative displacement of the 95th percentile Chinese human head / 医用生物力学

Objective To construct and validate the 95th percentile finite element model of Chinese human head, evaluate the relative displacement between the skull and brain according to four types of skull brain relative displacement experiments as references, and investigate the shear strain distributions on the brain. Methods By comparing the relative displacement curve derived from the experiment and simulation and referring to conclusions from animal and physical experiments, the position of higher shear strain was investigated. Results High correlations could be found between the simulated and experimental results for skull brain relative displacement. Peaks and troughs could always appear in curves of skull brain relative displacement due to the fact that the brain tissue tended to return to its initial state. The results that higher strain could occur at temporal lobe of the brain, corpus callosum and brain surface were consistent with conclusions from the animal and physical experiments. ConclusionsT he 95th percentile finite element model of Chinese human head is validated with good biofidelity. Due to the rotational motion of human head, higher shear strain will occur on brain surface and in deep brain, leading to injuries, which provids a scientific theoretical basis for the study on injury mechanism of human head in traffic accidents.