Chemical Constituents of Pericarps of Zanthoxylum bungeanum / 中国实验方剂学杂志

<b>Objective::To study the chemical constituents of pericarps of <italic>Zanthoxylum bungeanum</italic>. <b>Method::The dried pericarps of <italic>Z</italic>. <italic>bungeanum</italic> were smashed, and then extracted and concentrated in 95%ethanol to obtain the total extract. The total extract was loaded on a silica gel CC, eluted with different polar solvents in sequence, and then concentrated to obtain corresponding parts. The methylene chloride fraction and the <italic>n</italic>-butanol fraction were separated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, ODS column chromatography, semi-preparative HPLC, etc. And their structures were identified based on physicochemical properties and various spectroscopic methods. <b>Result::Fourteen compounds were isolated from the dichloromethane fraction and the n-butanol fraction of the <italic>Z</italic>. <italic>bungeanum</italic> and identified as(1<italic>S</italic>, 3<italic>S</italic>)-1-methyl-1, 2, 3, 4-tetrahydro-<italic>β</italic>-carboline-3-carboxylic acid(<bold>1</bold>), (3<italic>S</italic>)-1, 2, 3, 4-tetrahydro-<italic>β</italic>-carboline-3-carboxylic acid(<bold>2</bold>), apigenin-7, 4′-dimethyl ether(<bold>3</bold>), genkwanin(<bold>4</bold>), lcariside F₂(<bold>5</bold>), breyniaionoside A(<bold>6</bold>), 3-methoxyphenethyl alcohol-4-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopynanoside(<bold>7</bold>), 1-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopyranosyloxy-3-methoxy-5-hydroxybenzene(<bold>8</bold>), orcinol-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopyranoside(<bold>9</bold>), syringin(<bold>10</bold>), 4-[(3<italic>S</italic>)-3-hydroxybutyl]-2-methoxyphenyl-<italic>β</italic>-<italic>D</italic>-glucopyranoside(<bold>11</bold>), (+ )-lyoniresinol-3a-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopyranoside(<bold>12</bold>), 2-methylpropanyl-6-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-apiofuranosyl-<italic>β</italic>-<italic>D</italic>-glucopyranoside(<bold>13</bold>)and(<italic>E</italic>)-6-hydroxy-2, 6-dimethylocta-2, 7-dienoic acid(<bold>14</bold>). <b>Conclusion::All compounds were isolated from <italic>Z</italic>. <italic>bungeanum</italic> for the first time, and compounds <bold>1-4, 12</bold> and <bold>14</bold> were isolated from this genus for the first time.

Isolation and Identification of Antioxidant Activity Constituents from Elephantopus scaber / 中国实验方剂学杂志

Objective:To study the chemical constituents of 95% ethanol extract of the whole plant of Elephantopus scaber and investigate its antioxidant activity. Method:The chemical constituents were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography,ODS column chromatography,semi-preparative high performance liquid chromatography (HPLC) and recrystallization methods,while their structures were identified on the basis of nuclear magnetic resonance (NMR), mass spectrometry and comparison of their spectral data with those reported in the literature. Result:Fourteen compounds were isolated and their structures were identified as:(E)-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)-2(3H)-furanone (1),esculetin (2),dihydrosyringenin (3),(+)-isololiolide (4),caffic acid (5),luteolin-7-O-β-D-glucuronide (6),luteolin-7-O-β-D-glucuronide methyl ester (7),chlorogenic acid methyl ester (8),blumenol A (9),(6R,9R)-3-oxo-α-ionol-β-D-glucopyranoside (10),byzantionoside B (11),3,5-O-dicaffeoyl quinic acid methyl ester (12),3,4-O-dicaffeoyl quinic acid methyl ester (13) and 4,5-O-dicaffeoyl quinic acid methyl ester (14). Conclusion:Compounds 1-4 and 8-10 were isolated from the genus Elephantopus for the first time. Compound 11 was isolated from E. scaber for the first time. The DPPH radical and ABTS+ radical scavenging experiments on twelve of these compounds showed that compounds 1,2,5,6,12 and 13 had significant antioxidant activity.

Chemical Constituents from Periplaneta americana / 中国药学杂志

OBJECTIVE: To study the chemical constituents from Periplaneta americana. METHODS: The 95% enthanol extract was isolated and purified by column chromatography on silica gel, Sephadex LH - 20, ODS and semi-PHPLC. All compounds were identified by chemical and spectral analyses. RESULTS: Fourteen compounds were isolated and purified from Periplaneta americana. Their structures were elucidated as salicylic acid (1), benzoic acid (2), phenylacetic acid (3), methyl 4-hydroxyphenylacetate (4), p-hydroxybenzoic acid (5), genistein (6), 3-hydroxybenzoic acid (7), methyl N-(2-hydroxybenzoyl) glycinate (8), 2, 5 -dihydroxybenzoic acid (9), (2-hydroxybenzoyl) glycine (10), uracil (11), cyclo(Val-Ala) (12), N-acetyldopamine (13), and cyclo( L-Pro-L-Tyr) (14), respectively. CONCLUSION: Compounds 2, 4, 7 - 10, 12 and 14 are obtained from this genus for the first time.

Megastigmane glucosides isolated from Dichrocephala benthamii / 中国天然药物

The present study was designed to investigate the chemical constituents of the whole herb of Dichrocephala benthamii. A new megastigmane glucoside (compound 1), together with its four known analogues (compounds 2-5), was obtained. Their structures were elucidated on the basis of spectroscopic analyses (UV, IR, MS, and 1D and 2D NMR). The absolute configuration of compound 1 was assigned on the basis of CD method and chemical evidence. In addition, their cytotoxicity against human hepatoma cells (HepG-2) was evaluated by the MTT method. Compound 5 showed weak activity against HepG-2, while the other compounds did not show remarkable inhibitory effects.