Analysis of Genetic Variability, Correlation and Path for Yield and Its Attributing Traits in Wheat (Triticum aestivum L.)

The current study was undertaken to analyze the genetic variability, correlation and path for yield and its attributing traits at agriculture research farm, Department of Genetics and Plant Breeding, School of Agriculture, Lovely Professional University, Punjab during during Rabi 2021-22 and 2022-23. Augmented design with F2 seeds planted in ear to row fashion in 4 rows were used to study variability, correlation and path including 2 parent and its 6 F2’s for the 9 traits viz. plant height, spike length, number of tillers per plant, number of spikes per plant, number of spikelets per ear, number of grains per ear, biological yield, harvest index and grain yield. Finding revealed that grain yield revealed positive and highly significant correlation with number of tillers per plant (rp= 0.8387, rg= 0.8709) and biological yield (rp= 0.9671, rg= 0.9965) while positive and significant correlation with Number of spikes per plant (rp= 0.7168, rg= 0.7253) at both phenotypic and genotypic level. Biological yield per plant revealed highest positive direct effect (1.0642, 0.8700) with significant association with grain yield (0.9965, 0.9671) whereas Number of tillers reveals least positive direct effect on grain yield (0.0183, 0.0834) at both genotypic and phenotypic level. PCV was higher than GCV for all the traits indicated an influence of environment over the traits. Number of spikelets per ear recorded highest PCV and GCV followed by Grain yield. Heritability (h2b) found higher for grain yield (84.2) followed by Number of spikelets per ear (83.4), Number of Grains per ear (82.7) and Plant height (80.1). Genetic advance (GA) recorded higher Number of spikelets per ear (35.07) followed by Grain yield (11.93). Moderate GA recorded for Plant Height (7.67) and Biological yield (6.88) whereas Spike length (0.62) recorded lowest GA. Hence, selection for these mentioned characters could bring improvement in yield and yield components.

Performance & clinical utility of oropharyngeal versus nasopharyngeal swabs in COVID-19

Background & objectives: The oropharyngeal (OP) and nasopharyngeal (NP) swab samples are the most recommended clinical specimens for detecting SARS-CoV-2 in an individual through the quantitative real-time reverse-transcriptase-polymerase chain reaction (rRT-PCR) method. The primary objective of this study was to compare the performance of NP and OP swabs for the diagnosis of COVID-19 among 2250 concomitant samples (1125 NP + 1125 OP) using rRT-PCR test. Methods: This study was conducted at a tertiary care hospital in southern India. The study compared the specificity and efficacy of the two samples (NP & OP swabs) in 1125 individuals suspected having COVID-19 infection. The rRT-PCR values from all the samples were compared based on gender, age group and viral load. The differences between unmatched proportion and matched proportion were analysed. Agreement between the two methods was assessed using Kappa statistic. Absolute sensitivity, specificity, positive and negative predictive values (PPV and NPV) for OP and NP swabs were analysed. Results: The study identified a fair degree of agreement between OP and NP swabs in diagnosis of COVID-19 (kappa = 0.275, P<0.001). There was also a fair degree of agreement between NP and OP swabs irrespective of gender, age or duration of symptoms. NP swabs had better sensitivity and NPV as compared to OP swabs, however, specificity and PPV were 100 per cent for both. Interpretation & conclusions: The present study showed that both OP and NP swabs had similar sensitivity and specificity for predicting the presence of SARS-CoV-2.

Dynamics of the occurrence of influenza in relation to seasonal variation in Chennai, Tamil Nadu: A 7 -year cumulative study

Background: Influenza viruses have emerged as virulent pathogens causing considerable burden across the world. A thorough understanding of the pattern in occurrence of influenza globally is the need of hour. The present study deals with analysis of the dynamics of Influenza virus, especially the influence of seasonal change on viral circulation and causation of epidemics/pandemics in the context of subtropical region. Methods: During the 7 year (2009–2015) study, 36670 specimens were subjected to influenza analysis. Nasopharyngeal swabs collected from suspected patients from Chennai, Tamil Nadu, were tested and typed by real-time polymerase chain reaction assay. Results: During 2009 pandemic, among influenza A positives 95.16% were Apdm09, indicating that there was a predominant circulation of Apdm09. During postpandemic period, there were waves in the occurrence of Apdm09 which indicates fall in immunity with buildup in the susceptible population. Conclusion: In Chennai, Tamil Nadu, influenza positivity started with the onset of monsoon and peaks during the postmonsoon months throughout the study period. The assessment of meteorological factors compounding influenza activity can help in raising alerts to the public health officials of impending disaster which suggests that Influenza vaccination can be initiated before monsoon months in South India.

Poisoning Among Children In A Tertiary Care Centre Of Andhra Pradesh.

Abstracts: Background & Aim: Accidental Poisoning in children focuses on the effect of substances caused by accidental poisonings from drugs of abuse, household products, or various other chemicals. Aim is to analyze children with accidental poisoning for the epidemiological factors; type of poison, time elapsed to reach the hospital, mode of poisoning, socioeconomic status and outcome. Methodology: This was a hospital based prospective observational study carried out at department of pediatrics, SVRR Government General Hospital during the periods of November 2011 to October 2013 . Total of 127 children admitted in the hospital were studied. Results: Most common cause of poisoning in children was kerosene (37.8%) followed by organophosphates(24.4%). 54.3%of the poisoned cases were in the age group1month-5year, compared to 37% and 8.6% of the age groups 6-10 and 11-12 yr respectively. Overall incidence of poisoning in males was much higher than females, but in the age group above 10 years there is increased incidence in females. Conclusion: outcome of poisoning is directly related to the interval between poisoning and presentation to emergency care. Immediate access to the medical management , responsible monitoring of the poisoned patients, rapid treatment and follow-ups are essential to improve the condition of these poisoned children.

Taxonomic Implications with Special Reference to Stomatal Variations in Solanum Species Using Light and Scanning Electron Microscope.

The systematic analysis between 15 different species and one species with two accessions in Solanum has been carried out and need all over revisions and investigations. In continuation to our previous morphometric analysis, currently stomatal variation is analyzed using light microscopy and scanning electron microscopy. In addition to classical anomocytic and anisocytic stomatal types, new types of stomata (anisotricytic, paratetracytic, diacytic and paracytic) are noticed for the first time in the genus Solanum. The scanning electron microscopic study reveals the nature of stomatal pore, nature of peristomatal rims as well as the presence of epicuticular wax deposits and wax flakes which are of taxonomic significance. This study also indicates that foliar stomatal characteristics are valuable taxonomic traits, which can be utilized to address the taxonomic issues within the genus.

Cutaneous fungal infection in a renal transplantation patient due to a rare fungus belonging to order Pleosporales.

Fungal infections are being increasingly reported from immuno-compromised as well as immuno-competent patients. Transplant patients are on long term immunosuppressive therapy which makes them highly vulnerable to opportunistic fungal infections .These infections can be cutaneous or systemic. Several fungi have been reported to be the culprits such as Candida spp., Aspergillus spp., C. neoformans, P. carinii, and zygomycetes group of fungi. Cutaneous infections are most commonly caused by Pityriasis (tinea) versicolor, dermatophytes, and candida sp but these days the demtiaceous fungi are becoming more frequently reported .Here we report a case of post renal transplant cutaneous infection caused by dematiaceous fungus belonging to the order Pleosporales.

Larsen Syndrome: A Case Report.

Larsen syndrome was first described in 1950 by Larsen, Schottstaedt and Bost. This rare inherited disorder is characterized by congenital dislocation of multiple joints along with other anomalies of heart, face, hands and bones. Awareness of this condition and assosciated complications helps in better follow up and management of these patients.

Cell proliferation and natural killer cell activity by polyherbal formulation, Immu-21 in mice.

Immunomodulatory activity of an Ayurvedic polyherbal formulation, Immu-21 containing extracts of Ocimum sanctum, Withania somnifera, Emblica officinalis and Tinospora cordifolia was studied on proliferative response of splenic leukocytes to T cell mitogens, concanavalin (Con)-A and phytohemagglutinin (PHA) and B cell mitogen, lipopolysaccharide (LPS) in vitro by [3H]-thymidine uptake assay in mice. The cytotoxic activity of Immu-21 was tested by measuring the splenic leukocyte natural killer (NK) cell activity against K 562 cells. Intraperitoneal (i.p.) treatment with Immu-21 (30 mg/kg) once a day for 14 and 21 days did not cause change in body weight and spleen weight, where as splenocytes/spleen count was increased. Treatment of Immu-21 (30 mg/kg, i.p.) for 14 days and 1 mg/kg for 21 days significantly increased LPS induced leukocyte proliferation. NK cell activity was significantly increased when mice were pretreated with Immu-21 (10 and 30 mg/kg, i.p.) once a day for 7 days. The results indicate that pretreatment with Immu-21 selectively increased the proliferation of splenic leukocyte to B cell mitogen, LPS and cytotoxic activity against K 562 cells in mice.

RH-5992--an ecdysone agonist on model system of the silkworm Bombyx mori.

RH-5992 is a novel synthetic non-steroidal ecdysteroid agonist with a high selectivity towards Lepidopteran species. The effect of ecdysone agonist RH-5992 on larval period, larval weight, silk gland weight and haemolymph protein profile were examined in the model organism, the larvae of silkworm, Bombyx mori. The LD50 values were found to be 16.21 and 12.01 micrograms/larva for 72 and 96 hr respectively. In the present study, three sublethal concentrations of 1/5th, 1/10th and 1/20th of LD50 at 72 hr were selected and applied on the mid-dorsal line of the silkworm B. mori. The maximum mortality of 35% was observed in the group which received the highest (3.2 micrograms/larva) concentration of RH-5992. The mortality rate was found to be dose dependent as well as time dependent. Interesting results were observed in haemolymph profile of the RH-5992 treated larvae as staining intensity of 30 kDa protein decreased significantly whereas the effect was not marked on other major proteins like storage proteins and vitellogenin polypeptides. From the results, it is confirmed that RH-5992 causes changes in larval characters and protein profile of silkworm B. mori. It is proposed that RH-5992 may cause negative effect specifically on reproductive characters like development of ovary and egg production due to decrease in 30 kDa protein.

Left ventricular mass index in patients of end stage renal disease (non-diabetic) on regular haemodialysis.

Echocardiography in patients of end stage renal failure (non diabetic) on regular haemodialysis revealed increase in left ventricular mass index in comparison to healthy controls. Anaemia, chronic uraemic state and hypertension were the probable contributory factors.

Hepatic cysts in adult polycystic kidney disease.

Of ninety two adult polycystic kidney patients 18.5% had liver cysts. Mostly they were multiple. Liver cysts were more common in men.