Résumé
Objective The purpose of this study is to explore the applicability of chemiluminescent immunoassay in screening hepatitis C infection,and to find the infection status of heptitis C virus in Ningxia,and to provide scientific basis for its pre vention and control.Methods A total of 209 889 hepatitis C antibody (anti-HCV) data detected by chemiluminescent immu noassay (CIA) were collected from the General Hospital of Ningxia Medical University during 2011～2015.According to the 《Laboratory Hepatitis C Antibody Detection and Results Report Guide of 2003 CDC》of the United States,Cases were divid ed into weak positive (0.9≤S/CO value≤8.0) and positive (S/CO value＞8.0) two groups.According to the clinical diag nostic criteria of 《 Hepatitis C Prevention Guide》 of 2015 version to complete the diagnosis of acute and chronic hepatitis C.The chi square test and curve estimation of regression analysis was used to analyze the diagnosis rate of hepatitis C and the distribution characteristics of the patients with hepatitis C.Results In the S/CO value＞8.0 group,the diagnosis rate of hepatitis C was 97.57％.In the 0.9≤S/CO value≤8.0 group,the diagnosis rate of hepatitis C was only 2.05％.Among the people infected with HCV,there was no significant difference between male and female (x2 =1.432,P＞0.05).The infection rate was high in the individuals of ages between 21～60 years old,the infection rate of children and the elderly was low (x2 254.901,P＜0.01),almost all Departments of the hospital were distributed.The majority of false positive groups were elderly,children,pregnant women and tumor patients.The main departments were obstetrics,oncology,pediatrics and oral and maxillofacial surgery.Conclusion The method of Chemiluminescent immunoassay to detect anti-HCV is suitable for clinical screening of hepatitis C.but it is necessary to pay attention to the treatment of false positives.It is necessary to improve the detection of hepatitis C in the population with high prevalence in Ningxia,and pay attention to the prevention and treatment of nosoco-mial infection,and constantly improve the screening and monitoring system for hepatitis C.
Résumé
<p><b>OBJECTIVE</b>To investigate ESI-MS/MS fragmentation pattern of bile acids contained in Qingkailing injection, and the rapid identification method.</p><p><b>METHOD</b>HPLC-ESI-MS/MS technology was adopted to investigate ESI-MS/MS fragmentation pattern of five bile acids contained in Qingkailing injection, and rapidly identify bile acids contained in Qingkailing injection from eight different manufacturers.</p><p><b>RESULT</b>5 bile acids showed similar ESI-MS/MS fragmentation patterns, based on which 14 bile acids were rapidly separated from Qingkailing injection. Among them, 11 were found in injections of all of the manufacturers, and the rest three were found in individual manufacturers.</p><p><b>CONCLUSION</b>HPLC-ESI-MS/MS is used to rapidly identify bile acids from complex material systems, which provides an effective method for interpreting the complex material base of compound traditional Chinese medicine preparations and enhance the quality standards.</p>