Résumé
BACKGROUND: Endometritis is the most common disease of dairy cows and traditionally treated with antibiotics. Lactic acid bacteria can inhibit the growth of pathogens and also have potential for treatment of endometritis. Using PacBio single-molecule real-time sequencing technology, we sequenced the fulllength l6S rRNA of the microbiota in uterine mucus samples from 31 cows with endometritis, treated with lactic acid bacteria (experimental [E] group) and antibiotics (control [C] group) separately. Microbiota profiles taken before and after treatment were compared. RESULTS: After both treatments, bacterial species richness was significantly higher than before, but there was no significant difference in bacterial diversity. Abundance of some bacteria increased after both lactic acid bacteria and antibiotic treatment: Lactobacillus helveticus, Lactococcus lactis, Lactococcus raffinolactis, Pseudomonas alcaligenes and Pseudomonas veronii. The bacterial species that significantly decreased in abundance varied depending on whether the cows had been treated with lactic acid bacteria or antibiotics. Abundance of Staphylococcus equorum and Treponema brennaborense increased after lactic acid bacteria treatment but decreased after antibiotic treatment. According to COG-based functional metagenomic predictions, 384 functional proteins were significantly differently expressed after treatment. E and C group protein expression pathways were significantly higher than before treatment (p < 0.05). CONCLUSIONS: In this study, we found that lactic acid bacteria could cure endometritis and restore a normal physiological state, while avoiding the disadvantages of antibiotic treatment, such as the reductions in abundance of beneficial microbiota. This suggests that lactic acid bacteria treatment has potential as an alternative to antibiotics in the treatment of endometritis in cattle.
Sujets)
Animaux , Femelle , Bovins , Maladies des bovins/traitement médicamenteux , Endométrite/traitement médicamenteux , Lactobacillales/métabolisme , Séquençage nucléotidique à haut débit/méthodes , Antibactériens/administration et posologie , Bactéries/isolement et purification , Bactéries/croissance et développement , Bactéries/effets des médicaments et des substances chimiques , Utérus/microbiologie , ARN ribosomique 16S/génétique , Acide lactique , Lactobacillales/génétique , MicrobioteRésumé
The immune performance, SNPs and expression levels of candidate genes (IL1-β, Nramp1, TLR4, MyD88, NF-кB and NLRC5) were analyzed in carrier chickens of a Chinese indigenous breed infected with Salmonella enterica Serovar Pullorum at different persistence periods (12, 19 and 24 weeks of age). Carrier birds at 19 weeks of age presented significant difference in most immune parameters, as compared to carriers at 12 and 24 weeks of age, while no significant difference in most immune parameters was observed between carriers at 12 and 24 weeks of age. The genotype distributions of IL1-β and TLR4 presented significant differences between carriers and healthy birds. The expression levels of most candidate genes in carriers at 19 weeks of age were significantly higher than that in carriers at 12, 24 weeks of age and healthy birds and reached 1% level of significance between carriers at 19 weeks of age and healthy birds. The expression patterns of all genes, but IL-1β and NLRC5 between carriers at 12 and 24 weeks of age in all tissues were similar. Compared with carriers at 12 weeks of age, IL1-β was significantly down-regulated, but NLRC5 was significantly up-regulated in carriers at 24 weeks of age. Our study demonstrated that immune performance of carrier birds was severely impaired at age of sexual maturation and NLRC5 might play as a negative mediator of NF-кB pathway involved in immune response to asymptomatic infection by S. Pullorum. The TLR4/MyD88/NF-кB pathway might be suitable for study on S. Pullorum infection in Chinese indigenous breeds.
Sujets)
Animaux , Poulets/génétique , /génétique , /immunologie , Facteur de transcription NF-kappa B/génétique , Facteur de transcription NF-kappa B/immunologie , Salmonella enterica/génétique , Salmonella enterica/immunologieRésumé
<p><b>OBJECTIVE</b>To compare the long-term clinical effects of two kinds of crowns and bridges made of porcelain fused to Au-Pt alloy and Ni-Cr alloy.</p><p><b>METHODS</b>A total of 131 teeth (64 patients) were rehabilitated using porcelain-fused-to-metal crowns, among which 59 were rehabilitated with Au-Pt alloy metal ceramic crown and 72 with Ni-Cr alloy metal ceramic crown.The porcelain fracture, shade, marginal adaptation, gingival discoloration, and gingival status after finishing restoration and 36 months of follow-up were evaluated.</p><p><b>RESULTS</b>After 36 months of restoration, porcelain fused to Au-Pt alloy crown showed better clinical effects than porcelain fused to Ni-Cr alloy crown in shade, marginal adaptation, gingival discoloration, as well as gingival status (all P < 0.05).</p><p><b>CONCLUSION</b>The Au-Pt alloy ceramic crown is superior to Ni-Cr alloy ceramic crown in long-term clinical effects.</p>