Research progress in prevention and cure of glucagon-like peptide-1 receptor agonists-1 receptor agonists for diabetic retinopathy / 中华眼底病杂志

Diabetic retinopathy (DR) is one of the most frequent complications of diabetes (T2DM), which is the main eye disease causing blindness in adults in recent years. At present, glucagon-like peptide-1 receptor agonists (GLP-1RA) have become the main drugs used in the treatment of diabetes due to its superior hypoglycemic, lipid-lowering, hypertensive and cardiovascular effects. A large number of studies have shown that GLP-1RA drugs can protect retinal microvascular and optic nerves in the treatment of diabetes through various ways, but some studies have found that GLP-1RA drugs represented by semaglutide may lead to the progress of DR. Therefore, GLP-1RA should be used cautiously for patients who with severe non-proliferative DR or proliferative DR. Regardless of whether T2DM patients are complicated with DR, the fundus retinal condition should be monitored regularly after the use of GLP-1RA drugs, and timely countermeasures should be taken when DR occurs and develops. The benefits of GLP-1RA used by diabetes patients are obvious to all, and scientific and rational drug use can prevent the occurrence and progress of DR, which can better benefit DR Patients.

Current status of age-related eye diseases in elderly population and their visual function and visual-related quality of life / 中华全科医师杂志

Objective To explore the prevalence of age-related eye diseases (AREDs) among people aged 70 years or above in Hangzhou and evaluate the impact of AREDs on visual function (VF) and visual-related quality of life (QOL) in elders.Methods This study involved a total 2 111 elderly people (≥70 years).All participants received visual acuity and comprehensive eye examinations and complete VF and QoL questionnaires.Results The main cause of visual impairment was AREDs.And the causes were age-related cataract (79.82％),AMD (45.64％),glaucoma (10.95％) and diabetic retinopathy (DR,7.30％).VF and QoL scores declined gradually with age.And the scores declined more rapidly with declined visual acuity among the elders.VF and QoL scores in patients with age-related cataract,AMD,glaucoma and DR were successively lower.After adjusting for age,gender and visual acuity,the elders with AREDs had lower scores across all domains of VF and QoL.Scores for subscales of VF and QoL domains were significantly lower among those with DR and glaucoma compared with those with age-related cataract and AMD.Conclusion Age,presenting vision and AREDs are associated with VF and QoL in this elderly population.And senile people with glaucoma and DR have severe declines in VF and QoL,independent of presenting visual acuity.

Effects of subretinal fluid on proliferation of cultured human retinal pigment epithelial cells and fibroblast cells / 中华实验眼科杂志

ObjectiveTo detect the effect of subretinal fluid （SRF） from different grades of proliferative vitreoretinopathy（PVR） on cultured retinal pigmental epithelial cells（RPE） and fibloblasts（FB）．MethodsThe effect of SRF from different grades of PVR on RPE and FB was determined using Brdu labeling technique and MTT assaying method，respectively．Results All samples showed a stimulating effect on the two kinds of cellular elements in a certain degree．At 1∶10 concentration，the cellular proliferation stimulating activity of SRF with PVR＜C1，PVR C1 and PVR＞C1 on RPE and FB was 128.5%，139.8%，156.8%and 126.3%，143.1%，172.3%of the control groups, respectively after 24 h stimulation.ConclusionsSRF’s proliferation-stimulating activity is correlated with the grades of PVR．

Roles of subretinal fluid on expressio n of bcl-2 oncoprotein of cultured human retinal pigment epithelial cells / 中华眼底病杂志

Objective To investigate the effect of subretinalfluid (SRF) with different grades of proliferative vitreoretinopathy (PVR) onｂcl-2 oncoprotein expression in retinal pigment epithelium (RPE) cells and fib roblast (FB)．Methods Using immunohistologicalstain ingtechniｑue and Westernblotting method to detect the expression of ｂcl-2 protein in RPE cells and FB under the stimulation of SRF．Res u ltsThe expression levels of ｂcl-2 increased in both types oｆcells to a certain extent compared with those of the control group 4 hours afterthe cells subjected to SRF； 36 hours later, the expression levels of ｂcl-2 o f experimental groups decreased more quickly thanthose of the controlgroup， and the controlgroup showed relatively higher ｂcl-2 protein levels at the end of observation． Conclusions SRF may stimulate the e xpression of ｂcl-2 in RPE cells and FB under culture at early stage, but accel arate the declining of ｂcl-2 protein levels a certain time after subjected toSRF．

Roles of subretinal fluid on expression of bcl-2 oncoprotein of cultured human retinal pigment epithelial cells and fibroblast cells / 中华眼底病杂志

Objective To investigate the effect of subretinal fluid (SRF) with different grades of proliferative vitreoretinopathy (PVR) on bcl-2 oncoprotein expression in retinal pigment epithelium (RPE) cells and fibroblast (FB). Methods Using immunohistological staining technique and Western-blotting method to detect the expression of bcl-2 protein in RPE cells and FB under the stimulation of SRF. Results The expression levels of bcl-2 increased in both types of cells to a certain extent compared with those of the control group 4 hours after the cells subjected to SRF; 36 hours later, the expression levels of bcl-2 of experimental groups decreased more quickly than those of the control group,and the control group showed relatively higher bcl-2 protein levels at the end of observation. Conclusions SRF may stimulate the expression of bcl-2 in RPE cells and FB under culture at early stage, but accelarate the declining of bcl-2 protein levels a certain time after subjected to SRF.