Gene Expression Changes of Drug-resistant Saos-2 Cells Induced by Anticancer Drug / 대한해부학회지

During the treatment of cancers, especially with anticancer drugs, the recurrence of cancer is the most important factor for survival rate. The most common cause of the recurrence is the resistance of cells to anticancer drugs. To explore and analyze the changes of gene expression during the induction of resistance by anticancer drugs in human osteogenic cancer cell line Saos-2. The drug resistance was induced with adriamycin, cisplatin or vincristine at 10(-7) M concentration of each and cDNA microarray was performed. Total RNA was purified from Saos-2, adriamycin-resistant (Saos-2AdR), cisplatin-resistant (Saos-2CpR) and vincristine-resistant (Saos-2VcR) and expressed genes were investigated with a Affymetrix Human HG-U133Plus2.0 GeneChip(TM). The genes of anticancer drug resistant cells that showed more than 2.5 fold expression change than Saos-2 were selected for differential expression. Four hundred seventeen genes were selected for Saos-2 vs Saos-2AdR. Two thousand five hundred thirty six genes were selected for Saos-2 vs Saos-2CpR. Two hundred twenty five genes were selected for Saos-2 vs Saos-2VcR. Eighty seven genes were selected for common differential expression. The results showed that many genes were changed in expression during the acquiring of resistance to anticancer drugs but most of genes were not in common among the three anticancer durg-resistant Saos-2. This means the different anticancer drug takes the different mechanism for acquiring resistance to anticancer drug even we use same cells.

Profile of Gene Expression Changes During Doxorubicin Induced Apoptosis of Saos-2 / 영남의대학술지

BACKGROUND: Doxorubicin has proved to be a useful chemotherapeutic agent especially for osteogenic sarcoma. It induces cancer cell death via apoptosis. MATERIALS AND METHODS: To explore and analyze the changes of gene expression during doxorubicin induced apoptosis on human osteogenic sarcoma, Saos-2 cell, cDNA microarray was performed. After treatment with doxorubicin, total RNA was purified and expressed genes were investigated with a 17k human cDNA microarray. RESULTS: For analysis of the cDNA microarray, the genes were filtered using the sum of the median value of Cy3 and Cy5 signal intensity of greater than 800. Expression of 264 genes was changed by more than 2 fold, and the expression of 35 genes was changed more than 3 fold after treatment with doxorubicin. The genes were primarily related to cell death, cell growth and maintenance, signal transduction, cellular component, transport, and metabolism. CONCLUSION: Treatment with doxorubicin induced expressional change of many genes. Some of the genes might be related with apoptosis directly or indirectly. Further study is now needed to characterize these genes.