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Objective @#To explore the mechanism of motor symptoms in Parkinson' s disease ( PD) aggravated by decreased glial cell line-derived neurotrophic factor ( GDNF) in striatum．@*Methods @#Male C57 / BL mouse (6 －8 weeks) ，were administered of PBS，AAV-GDNF or AAV-shGDNF in striatum by brain stereoscopic injection，com- binated with sub-acute PD model ，1-Methyl-4-phenyl-1，2，3，6-tetrahydropyridine ( MPTP) 30 mg / ( kg · d) was administered through intraperitoneal injection for five consecutive days ; subsequently，mice were randomly divided into PBS group，negative control ( NC) group，AAV-shGDNF group ，MPTP group ，MPTP + AAV-GDNF group， MPTP + AAV-shGDNF group．Behavior tests (rotarod，pole and field) were applied for assessing the motor ability of mice ; ELISA kit was used to detect striatal glutamic acid ( Glu) content ; Western blot and other techniques were carried out to detect the expression and distribution of GLAST，GLT-1 and GluN2B in striatum ; TUNEL stainingwas applied for observing the apoptosis of neurons in striatum. @*Results @#Compared with the NC group，the mice of the AAV-shGDNF group，with down-regulation of GDNF in striatum，had poor motor ability，decreased Glu trans- porter ( GLAST and GLT-1) ，and increased Glu content． Compared with the PBS group，the mice of the MPTP group had increased Glu content and decreased GluN2B in striatum．Compared with the MPTP group，the mice of the MPTP + AAV-GDNF group showed enhanced motor ability ，along with decreased Glu content ，increased GluN2B and less neurons apoptosis in striatum ; while，the mice of the MPTP + AAV-GDNF group showed worse motor ability，along with augmented Glu content，reduced GluN2B and more neurons apoptosis in striatum．@*Conclusion@#In PD pathological process ，decreased striatal GDNF may promote the neurons apoptosis by enhancing Glu excitotoxicity，thereby leading to the aggravation of motor symptoms.
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Mantle cell lymphoma is a B-cell malignancy with unique biological, pathological and clinical characteristics, accounting for about 5%-10% of non-Hodgkin lymphoma (NHL), and most patients are diagnosed at advanced stage. Mantle cell lymphoma has the aggressive characteristic of aggressive lymphoma and the refractory characteristic of indolent lymphoma, and the prognosis of patients is poor. In recent years, with the development of high-dose chemotherapy, autologous hematopoietic stem cell transplantation and new drug research, the survival time of patients has been significantly prolonged.
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Objective:To explore the effect of continuous obstetric-pediatric cooperative nursing on anxiety, depression and the parenting sense of competence in NICU premature mothers.Methods:Totally 140 mothers of NICU preterm infants in hospital from January 2018 to December 2018 were selected and randomly divided into the control group and intervention group according to the method of random number table, and each group 70 cases. The control group received the routine nursing mode in obstetrics and paediatrics and the intervention group received the continuous obstetric-pediatric cooperative nursing based on routine nursing. To compare the anxiety level, depression level, the score of parenting sense of competence, hospitalization days of preterm infants, the outpatient rate and the readmission rate of preterm infants within 1 months of discharge between two groups.Results:The levels of anxiety and depression of the mothers of premature infants in the intervention group during hospitalization ,1 week after discharge and 1 month after discharge were (43.17±6.89), (8.96±2.67), (34.65±6.87), (7.65±1.92), (34.97±6.06), (6.29±1.54) points, lower than control group (47.03±8.06), (10.97±3.34), (45.44±7.72), (9.43±2.61), (41.46±7.26), (8.28±2.23) points, the differences between the two groups showed statistical significance ( t value was 2.331-6.097, P<0.05). The two dimensions of parenting efficacy and parenting satisfaction were (31.47±4.97), (43.38±4.21) points, which were higher than (25.91±5.42), (39.43±5.91) points of the control group, there were significant statistical differences among the two groups ( t value was -6.314 and -4.528, P<0.01). The hospitalization days of premature infants, outpatient and readmission rate within 1 month of discharge were (13.25±3.27) days, 8.57% (6/70), 2.86% (2/70), which were shorter or lower than (15.59±2.79) days, 24.29% (17/70), 12.86% (9/70) in the control group, the differences were statistically significant ( t value was 2.909, χ2 values were 6.295, 4.834, P<0.05). There were significant statistical differences among interaction effect, time effect and intergroup effect ( F values were 4.921-99.310, P<0.05). Conclusion:Obstetrical-pediatric cooperative nursing can effectively to lower the anxiety and depression level of preterm mothers, improve the parental competence, promote the mental health of preterm mothers, and ultimately shorten hospital days and decrease the rate of outpatient and readmission of premature infants, which is conducive to preterm infants′ rehabilitation.
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Staphylococcus aureus (SAU) is one of the most common pathogens in nosocomial infections, and it often appears in the nasal vestibule, and the antibiotics resistance problemshas become more and more serious.Asymptomaticcolonization with SAU is a high risk factor that causes nosocomial infections, and it′s also the most easily neglected infection prevention and control links in clinical care. With the in-depth understanding of the colonization of the bacteria, the related epidemiological and decolonization nursing reports are gradually increasing. This review summarizes the clinically relevant aspects of nasal carriage of S. aureus, the relationship between colonization and nosocomial infection,and the nursing of decolonization. Which may provide guidance for prevention of nosocomial infections.
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Objective:To explore and discuss the differences of pregnancy contents and inform formats in in-formed consent form ( ICF) for the drug clinical trial between China and foreign countries. Methods:We collected Chinese and foreign ICFs for drug clinical trial that had been audited by the Ethics Committee of the third Xiangya Hospital for the past five years. Based on the relevant domestic and foreign law, we concluded the element stand-ards and inform formats about pregnancy inform. By analyzing the integrity of the whole elements, the inform rate of every element and the using rate of every inform format, we compared the differences of pregnancy contents and in-form formats between Chinese ICFs and foreign ICFs. Results:The total number of ICFs was 177 in this study, in-cluding 107 Chinese ICFs and 70 foreign ICFs. The integrity rate of pregnancy in Chinese ICFs was statistically lower than them in foreign ICFs (19% vs. 56%, P=0. 000). Compared with foreign ICFs, the low informed ele-ments were the study of the pregnancy risk (32% vs. 73%, P=0. 000), the pregnancy test during the following-up period (33% vs. 56%, P=0. 002) and the measurements for contraception (22% vs. 53%, P=0. 000). Conclusion:The integrity level of pregnancy content in Chinese ICFs was lower than that of the foreign ICFs. And the three elements including pregnancy risk study, pregnancy test during the following-up period and measure-ments for contraception was obviously defected. Pregnancy informing forms of informed consent in China was inferi-or to abroad.
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To investigate the application of seminar teaching on postgraduate education of ophthalmology,postgraduate from Grade 2008 to Grade 2010 were divided into experiment groups and control groups.Seminar teaching and conventional teaching were applied to two groups respectively.The performances of seminar teaching groups at each grade were better than the control groups ( P ＜ 0.05 ).Thus,seminar teaching can enhance theory and operation ability of postgraduate.