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1.
Protein & Cell ; (12): 874-887, 2023.
Article de Anglais | WPRIM | ID: wpr-1010762

RÉSUMÉ

The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has been widely used for genome engineering and transcriptional regulation in many different organisms. Current CRISPR-activation (CRISPRa) platforms often require multiple components because of inefficient transcriptional activation. Here, we fused different phase-separation proteins to dCas9-VPR (dCas9-VP64-P65-RTA) and observed robust increases in transcriptional activation efficiency. Notably, human NUP98 (nucleoporin 98) and FUS (fused in sarcoma) IDR domains were best at enhancing dCas9-VPR activity, with dCas9-VPR-FUS IDR (VPRF) outperforming the other CRISPRa systems tested in this study in both activation efficiency and system simplicity. dCas9-VPRF overcomes the target strand bias and widens gRNA designing windows without affecting the off-target effect of dCas9-VPR. These findings demonstrate the feasibility of using phase-separation proteins to assist in the regulation of gene expression and support the broad appeal of the dCas9-VPRF system in basic and clinical applications.


Sujet(s)
Humains , Activation de la transcription , RNA, Guide, CRISPR-Cas Systems , Régulation de l'expression des gènes , Systèmes CRISPR-Cas/génétique
2.
Protein & Cell ; (12): 316-335, 2022.
Article de Anglais | WPRIM | ID: wpr-929165

RÉSUMÉ

Recent advances in genome editing, especially CRISPR-Cas nucleases, have revolutionized both laboratory research and clinical therapeutics. CRISPR-Cas nucleases, together with the DNA damage repair pathway in cells, enable both genetic diversification by classical non-homologous end joining (c-NHEJ) and precise genome modification by homology-based repair (HBR). Genome editing in zygotes is a convenient way to edit the germline, paving the way for animal disease model generation, as well as human embryo genome editing therapy for some life-threatening and incurable diseases. HBR efficiency is highly dependent on the DNA donor that is utilized as a repair template. Here, we review recent progress in improving CRISPR-Cas nuclease-induced HBR in mammalian embryos by designing a suitable DNA donor. Moreover, we want to provide a guide for producing animal disease models and correcting genetic mutations through CRISPR-Cas nuclease-induced HBR in mammalian embryos. Finally, we discuss recent developments in precise genome-modification technology based on the CRISPR-Cas system.


Sujet(s)
Animaux , Systèmes CRISPR-Cas/génétique , ADN/génétique , Embryon de mammifère/métabolisme , Endonucleases/métabolisme , Édition de gène , Mammifères/métabolisme
3.
Protein & Cell ; (12): 811-822, 2017.
Article de Anglais | WPRIM | ID: wpr-756922

RÉSUMÉ

β-Thalassemia is a global health issue, caused by mutations in the HBB gene. Among these mutations, HBB -28 (A>G) mutations is one of the three most common mutations in China and Southeast Asia patients with β-thalassemia. Correcting this mutation in human embryos may prevent the disease being passed onto future generations and cure anemia. Here we report the first study using base editor (BE) system to correct disease mutant in human embryos. Firstly, we produced a 293T cell line with an exogenous HBB -28 (A>G) mutant fragment for gRNAs and targeting efficiency evaluation. Then we collected primary skin fibroblast cells from a β-thalassemia patient with HBB -28 (A>G) homozygous mutation. Data showed that base editor could precisely correct HBB -28 (A>G) mutation in the patient's primary cells. To model homozygous mutation disease embryos, we constructed nuclear transfer embryos by fusing the lymphocyte or skin fibroblast cells with enucleated in vitro matured (IVM) oocytes. Notably, the gene correction efficiency was over 23.0% in these embryos by base editor. Although these embryos were still mosaic, the percentage of repaired blastomeres was over 20.0%. In addition, we found that base editor variants, with narrowed deamination window, could promote G-to-A conversion at HBB -28 site precisely in human embryos. Collectively, this study demonstrated the feasibility of curing genetic disease in human somatic cells and embryos by base editor system.


Sujet(s)
Femelle , Humains , APOBEC-1 Deaminase , Génétique , Métabolisme , Séquence nucléotidique , Blastomères , Biologie cellulaire , Métabolisme , Systèmes CRISPR-Cas , Embryon de mammifère , Métabolisme , Anatomopathologie , Fibroblastes , Métabolisme , Anatomopathologie , Édition de gène , Méthodes , Expression des gènes , Cellules HEK293 , Hétérozygote , Homozygote , Mutation ponctuelle , Culture de cellules primaires , Régions promotrices (génétique) , Analyse de séquence d'ADN , Globines bêta , Génétique , Métabolisme , bêta-Thalassémie , Génétique , Métabolisme , Anatomopathologie , Thérapeutique
4.
Protein & Cell ; (12): 601-611, 2017.
Article de Anglais | WPRIM | ID: wpr-756965

RÉSUMÉ

Targeted point mutagenesis through homologous recombination has been widely used in genetic studies and holds considerable promise for repairing disease-causing mutations in patients. However, problems such as mosaicism and low mutagenesis efficiency continue to pose challenges to clinical application of such approaches. Recently, a base editor (BE) system built on cytidine (C) deaminase and CRISPR/Cas9 technology was developed as an alternative method for targeted point mutagenesis in plant, yeast, and human cells. Base editors convert C in the deamination window to thymidine (T) efficiently, however, it remains unclear whether targeted base editing in mouse embryos is feasible. In this report, we generated a modified high-fidelity version of base editor 2 (HF2-BE2), and investigated its base editing efficacy in mouse embryos. We found that HF2-BE2 could convert C to T efficiently, with up to 100% biallelic mutation efficiency in mouse embryos. Unlike BE3, HF2-BE2 could convert C to T on both the target and non-target strand, expanding the editing scope of base editors. Surprisingly, we found HF2-BE2 could also deaminate C that was proximal to the gRNA-binding region. Taken together, our work demonstrates the feasibility of generating point mutations in mouse by base editing, and underscores the need to carefully optimize base editing systems in order to eliminate proximal-site deamination.


Sujet(s)
Animaux , Humains , Souris , APOBEC-1 Deaminase , Génétique , Métabolisme , Protéines bactériennes , Génétique , Métabolisme , Séquence nucléotidique , Protéine-9 associée à CRISPR , Systèmes CRISPR-Cas , Cytidine , Génétique , Métabolisme , Transfert d'embryon , Embryon de mammifère , Endonucleases , Génétique , Métabolisme , Édition de gène , Méthodes , Cellules HEK293 , Séquençage nucléotidique à haut débit , Souris de lignée C57BL , Microinjections , Plasmides , Chimie , Métabolisme , Mutation ponctuelle , Génétique , Métabolisme , Thymidine , Génétique , Métabolisme , Zygote , Métabolisme , Transplantation
5.
Protein & Cell ; (12): 363-372, 2015.
Article de Anglais | WPRIM | ID: wpr-757583

RÉSUMÉ

Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious knowledge gap remains in our understanding of DNA repair mechanisms in human early embryos, and in the efficiency and potential off-target effects of using technologies such as CRISPR/Cas9 in human pre-implantation embryos. In this report, we used tripronuclear (3PN) zygotes to further investigate CRISPR/Cas9-mediated gene editing in human cells. We found that CRISPR/Cas9 could effectively cleave the endogenous β-globin gene (HBB). However, the efficiency of homologous recombination directed repair (HDR) of HBB was low and the edited embryos were mosaic. Off-target cleavage was also apparent in these 3PN zygotes as revealed by the T7E1 assay and whole-exome sequencing. Furthermore, the endogenous delta-globin gene (HBD), which is homologous to HBB, competed with exogenous donor oligos to act as the repair template, leading to untoward mutations. Our data also indicated that repair of the HBB locus in these embryos occurred preferentially through the non-crossover HDR pathway. Taken together, our work highlights the pressing need to further improve the fidelity and specificity of the CRISPR/Cas9 platform, a prerequisite for any clinical applications of CRSIPR/Cas9-mediated editing.


Sujet(s)
Humains , Blastocyste , Systèmes CRISPR-Cas , Hémoglobines anormales , Génétique , Métabolisme , Zygote
6.
Protein & Cell ; (12): 194-202, 2014.
Article de Anglais | WPRIM | ID: wpr-757510

RÉSUMÉ

Pluripotent stem cells (PSCs) have the potential to produce any types of cells from all three basic germ layers and the capacity to self-renew and proliferate indefinitely in vitro. The two main types of PSCs, embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), share common features such as colony morphology, high expression of Oct4 and Nanog, and strong alkaline phosphatase activity. In recent years, increasing evidences suggest that telomere length represents another important internal factor in maintaining stem cell pluripotency. Telomere length homeostasis and its structural integrity help to protect chromosome ends from recombination, end fusion, and DNA damage responses, ensuring the divisional ability of mammalian cells. PSCs generally exhibit high telomerase activity to maintain their extremely long and stable telomeres, and emerging data indicate the alternative lengthening of telomeres (ALT) pathway may play an important role in telomere functions too. Such characteristics are likely key to their abilities to differentiate into diverse cell types in vivo. In this review, we will focus on the function and regulation of telomeres in ESCs and iPSCs, thereby shedding light on the importance of telomere length to pluripotency and the mechanisms that regulate telomeres in PSCs.


Sujet(s)
Animaux , Humains , Modèles biologiques , Cellules souches pluripotentes , Métabolisme , Telomerase , Métabolisme , Télomère , Métabolisme , Homéostasie des télomères
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