Clinicopathological features of adult Wilms tumor with BRAF V600E mutation / 中华病理学杂志

Objective: To identify BRAF V600E mutations in adult Wilms tumor (WT) with overlapping histologic features of metanephric adenoma (MA) and to investigate the clinicopathological features of adult WT. Methods: The clinical features of adult WT diagnosed at the Fudan University Shanghai Cancer Center, Shanghai, China from 2012 to 2021 were reviewed. HE-stained slides of all cases were reviewed by 2 expert pathologists. Representative tissues were selected for BRAF V600E immunohistochemical (IHC) staining and gene sequencing. Results: In adult WT with MA-like areas (cohort Ⅰ, n=6), 5 of the 6 cases were composed of epithelial-predominant and were positive for WT-1 and CD56, respectively, and all were positive for CD57. All 6 cases revealed highly variable Ki-67 indices, ranging from 1% in some areas to 60% in others. 5 of the 6 cases harbored a BRAF V600E mutation. All cases in cohort I were followed up for 23 to 71 months, and all survived. In classical adult WT without MA-like areas cohort (cohort Ⅱ, n=13), all 7 cases with available material were negative for BRAF by IHC and none of them had any BRAF mutation. Conclusions: BRAF V600E mutations are frequently present in adult WT with overlapping morphologically features of MA, but not in those without. More importantly, adult WTs with overlapping histologic features of MA may be an intermediate entity between typical MA and WT that may have a favorable prognosis and possible therapeutic targets.

Microbial community changes in contaminated soils in response to phenanthrene amendment

Leachate and reclaimed wastewater have become the important sources of polycyclic aromatic hydrocarbons in soils. However, the information on bioremediation of leachate and reclaimed wastewater-contaminated soils is still lacking. Identification of changes in microbial structure or of enriched genera related to biodegradation could aid identification of particular organisms or consortia capable of degrading polycyclic aromatic hydrocarbons in these contaminated soils. In this study, terminal restriction fragment length polymorphism, coupled with 16S Ribosomal ribonucleic acid clone library analysis, was applied to investigate the composition of bacterial community in leachate-contaminated soil or grassland soil irrigated reclaimed wastewater and the response to phenanthrene amendment. Results showed that phenanthrene amendment had significant but different impacts on microbial community structure, dependent on soil source. Several greatly enriched terminal restriction fragments with phenanthrene biodegradation were identified. Moreover, genus rhizobacteria, possibly linked to phenanthrene biodegradation, was firstly reported in this study. This work might provide some new insights into bioremediation of polycyclic aromatic hydrocarbons-contaminated soils