RÉSUMÉ
Introduction: Type 2 diabetes is one of the most common metabolic diseases. Nitrate has been introduced as a new therapeutic agent for type 2 diabetes. Considering that both diabetes and nitrate have some effects on blood cell count and 30% of diabetic patients have anemia, the aim of this study was to determine the effect of sodium nitrate on blood cell count in obese type 2 diabetic rats
Materials and Methods: Forty-eight male Wistar rats were divided into four groups: Control, Control + nitrate, Diabetes and Diabetes + nitrate. The groups that received nitrate [Control + nitrate, Diabetes + nitrate] again were divided into two subgroups, which received sodium nitrate [100 and 250 mg/L in drinking water] for two months: control+nitrate100 [CN100], control+nitrate 250 [CN250], diabetes+nitrate100 [DN100], and diabetes+nitrate250 [DN250]. Diabetes was induced using a high-fat diet for 14 days and injection of streptozotocin. Blood cell count was performed at the end of the study
Results: In diabetic rats, nitrate administration reduced body weight, blood glucose, hematocrits, and neutrophils [all p<0.05] but increased total number of white blood cells and lymphocytes [p<0.05]. Nitrate administration had no effect on the number of red blood cells, hemoglobin concentration, MCV, MCH, MCHC, or platelet numbers
Conclusion: Administration of sodium nitrate, which is considered as a therapeutic agent in type 2 diabetes, decreased blood glucose in the type 2 diabetic rats but had no major harmful effects on blood parameters; in addition, it may also have anti-inflammatory effects by decreasing the number of neutrophils
RÉSUMÉ
Introduction: diabetes, one of the most common chronic diseases worldwide, has many complications and current treatments impose a high cost on health system necessitating that newer treatments be investigated. Type 2 diabetes mellitus is the most common form of diabetes, characterized by insulin resistance and dysfunction of pancreatic beta cells. Considering the restrictions of human studies, animal models of diabetes are appropriate tools for researches. Rodents are the first choice for inducing diabetes mellitus due to their short generation time and economic considerations. There are several animal models of type 2 diabetes, of which, those with both beta cell impairment and insulin resistance are preferable. In the high fat diet-streptozotocin model, as a model of type 2 diabetes, high fat diet induces insulin resistance and streptozotocin causes partial beta cell destruction. The high fat diet-streptozotocin model is a cost-effective and appropriate model for studying type 2 diabetes. In conclusion high fat diet-streptozotocin model has metabolic characteristics similar to those of human type 2 diabetes and can be used for investigating the effects of more recent antidiabetic medications available for the condition