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Objective To establish normal reference values for left ventricular trabecular and papillary muscle mass(TPMM)in Chinese adults using MRI and to explore its impact factors.Methods A total of 168 healthy Chinese adults were retrospectively included,and compacted and total left ventricular myocardial mass(LVM)were measured using traditional and dedicated methods,respectively.TPMM was calculated from the difference between total and compacted LVM.Independent sample t-tests and analysis of variance were used to explore the differences in TPMM among genders and age groups,while multiple linear regression was used to explore the independent correlation between TPMM and age,gender,heart rate,systolic blood pressure(SBP),fasting blood glucose(FBG),and body mass index(BMI).Results TPMM for men was significantly larger than that for female(P<0.001).TPMM in the elderly group was significantly larger in female(P<0.05),but not in men.Multiple linear regression showed that BMI and SBP were both independently positively correlated with TPMM,and female and heart rate were independently negatively correlated with TPMM(P<0.05).Conclusion This study provides age-and gender-specific normal reference values for TPMM in Chinese adults.Gender,heart rate,BMI,and SBP are all independently associated with TPMM.
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Objective:To evaluate the application value of patient-based real-time quality control (PBRTQC) algorithms in intralaboratory comparison between various hematology analyzers.Method:From April 1 st 2020 to March 31 th 2021, data of white blood cell (WBC) counts and daily comparison results of fresh venous blood, measured by five hematology analyzers, were collected at the Department of Laboratory Medicine in Hebei Children′s Hospital. First, the professional intelligent PBRTQC software system was applied to conduct the parameter setting, program establishment, and performance verification. Three concentration ranges of WBC were selected, low concentration (2.5-4.5)×10 9/L, medium concentration (6.0-8.0)×10 9/L and high concentration (12.0-14.0)×10 9/L for the comparison. Next, WBC counts were calculated with both of the EWMA and median methods, the results were then analyzed by PBRTQC using the module of"intralaboratory comparison of hematology analyzers". Finally, bias of intralaboratory comparison among various hematology analyzers analyzed by means of EWMA and daily comparison results of fresh venous blood were compared. Based on the standard of WS/T 406-2012,allowable error ±7.50% in WBC counts was set as the relative bias standard among different instruments. Results:(1) A total of 38 313 sample results were included, there were 70 warning results out of these samples based on the EWMA quality control method established on the data of patients with white blood cell count in our laboratory, with an early warning rate of 0.183‰, a probability of error detection of 100%, and a probability of false loss of control of 0. EWMA quality control efficiency met the quality objectives. (2) In the comparison monitoring of the results of 5 blood cell analyzers at high concentrations, the coincidence rate between EWMA and median method were both 100% (46/46) in weekly and monthly comparison, and EWMA could maintain a relatively stable monitoring efficiency in daily comparison. (3) In the selected natural month, the consistency rate between EWMA method and fresh blood comparison method was 95.24% (20/21).Conclusion:PBRTQC can be used as a valuable supplementary tool of IQC to continuously and effectively monitor the consistency of data derived from intralaboratory hematology analyzers with different bands and types, which can not only reduce the risk of quality and operating costs, but also improve the efficiency of laboratory management.
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Objective@#To explore sex differences in 3D T1texture features in the progression of Alzheimer's disease (AD) and to predict the diagnosis of AD patients of different sex．@*Methods@#Seventy-seven AD patients (34 males and 42 females) ，74 amnestic mild cognitive impairment ( aMCI) patients ( 35 males and 39 females) and 75 healthy controls (HC) (35 males and 40 females) were recruited and high-resolution 3-dimensional T1 structural images were collected． Brain regions closely related to AD brain damage were selected as regions of interest ( ROIs) ，texture feature extraction and feature screening were performed．Analyses were performed by sex，and the support vector machine (SVM) was used for classification and prediction. @*Results @#In the AD vs HC，AD vs aMCI and aMCI VS HC groups by different sex，we obtained some brain regions with relatively high recognition index in different subgroups，and found that there were significant differences between female patients and male patients with high recognition index，and the recognition index of female patients ( area under the curve，accuracy，sensitivity and specificity were generally higher than that of male．@*Conclusion@#There are significant sex differences in texture features in AD process，and the classification and prediction ability of texture features in female patients is better， suggesting the importance of sex differences in AD research．This study provides some reliable biomarkers for early sex-specific identification of AD，which may be helpful for the early diagnosis and treatment of AD in the future.
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Objective:To explore the effect of visual processing patterns on emotional face processing in patients with Alzheimer's disease (AD).Methods:From June 2020 to August 2021, twenty-two AD patients (AD group) who met the conditions of this study were selected from the memory impairment clinic of the First Affiliated Hospital of Anhui Medical University, and demographically matched twenty-one elderly healthy people (control group) were selected from the patients' family members and community residents. The two groups of subjects performed emotional face visual scanning and facial recognition experiments after completing the evaluation of the cognitive scale and eye movement data were recorded in the emotional face visual scanning task. Statistical analysis of the obtained results was performed using SPSS 23.0 Windows version software. The data that conformed to the normal distribution were tested by independent samples t-test and variance analysis, and the data that did not conform to the normal distribution were tested by nonparametric test. Results:(1)In the emotional face recognition task, the total accuracy of facial emotion recognition of AD patients(0.52(0.42, 0.59)) was lower than that of the normal control group(0.67(0.64, 0.69)), and the difference was statistically significant( Z=-4.023, P<0.01), which was mainly manifested in recognizing complex facial emotion. (2) In the emotional face visual processing task, the saccade count ((1.96±0.97), (2.50±0.44)), fixation count ((3.93±2.58), (6.37±2.08))and fixation time ((1 205.89±727.32)s, (1 761.38±525.54)s)of AD patients were lower than those of the control group( t=-2.314, -3.402, -2.880, all P<0.05), and the surrounding facial fixation time (384.95 (276.51, 587.78)s, 276.06 (190.03, 384.55)s) was higher than that of the control group( Z=-2.478, P=0.013). Patients with AD had a lower fixation count than that in the control group on the eye area of surprise ((3.76±2.90), (6.25±2.19)), anger ((4.48±2.72), (7.06±2.55)) and disgust ((4.10±2.45), (6.67±2.45)), and the differences were statistically significant ( t=-3.164, -3.207, -3.436, all P<0.05). Patients with AD had a lower fixation time than those of the control group on the eye area of surprise ((1 150.26±753.22)s, (1 779.91±551.66)s), angry ((1 430.85±869.52)s, (1 944.51±612.63)s) and disgust ((1 266.14±765.67)s, (1 898.33±676.02)s), and the differences were statistically significant ( t=-3.115, -2.247, -2.865, all P<0.05). (3) Spearman correlation analysis showed that the accuracy of overall emotional face recognition was positively correlated with the fixation time in the eye area in AD patients ( r=0.429, P<0.05). Conclusion:The impaired visual processing of AD patients causes emotional face recognition disorders. Therefore, AD patients have different visual processing patterns in emotional face processing than age-matched normal controls, mainly manifested as the decreased fixation on the eye area.
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Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of large amounts of autoantibodies and the involvement of multiple organs. The polarization imbalance and abnormal activation of macrophages are closely related to the occurrence and development of SLE, and M1 macrophages, specifically, play a pro-inflammatory role in the pathogenesis of SLE. Changes in the immune microenvironment of SLE patients would promote the metabolic reprogramming of macrophages, and induce fluctuations in sugar metabolism, lipid metabolism, and amino acid metabolism, which lead to the accumulation of intermediate metabolites that would act as inflammatory signaling molecules to aggravate the inflammatory response and cause a series of complications. Therefore, a full understanding of the metabolic process of macrophages in SLE will help elucidate the pathogenesis of SLE and lay the foundation for targeted therapy of macrophages.
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@#To observe the effects of cannabidiol（CBD）on cognitive function and AMPA receptor expression in sporadic Alzheimer’s disease（sAD）rats induced by bilateral intraventricular injection of streptozotocin（STZ）. MethodsForty adult male Sprague-Dawley（SD）rats were randomly divided into four groups：sham operation group（S），cannabidiol（CBD）+ sham operation group（CBD+S），model group（STZ），CBD+model group（CBD+STZ）. The cognitive function of rats was tested by Morris water maze（MWM）and step-down test. Western blot was used to detecte the expression of GluR1 and GluR2 in the cerebral cortex and hippocampus. ResultsIn the Morris water maze test，compared with the S group，the escape latency in the STZ group was significantly prolonged（P<0.01），and the escape latency in the CBD+STZ group was significantly shortened（P<0.01）. In the step-down test，compared with the latency and the number of errors in the S group，the latency in the STZ group was significantly shortened（P<0.01），and the number of errors was significantly increased（P<0.01）. Compared with the STZ group，the latency of CBD+STZ group was significantly prolonged（P<0.05），and the number of errors of CBD+STZ group was significantly reduced（P<0.05）. Compared with the STZ group，the contents of GluR1 in cortex and hippocampus of the CBD+STZ group were significantly（P<0.01）（P<0.05）decreased. Compared with STZ group，the contents of GluR2 in cortex and hippocampus of the CBD+STZ group were significantly（P<0.01）（P<0.01）decreased. ConclusionCannabidiol improves STZ-induced cognitive impairment in rats by regulating AMPA receptor expression in the brain.
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Objective:To detect IgG and neutralizing antibodies response to SARS-CoV-2 vaccine by comparing enzyme-linked immunosorbent assay (ELISA), commercial magnetic particle chemiluminescence assay(CLIA) and neutralization test(NT).Methods:ELISA, CLIA and NT were used to detect 143 healthy people before and after 28 days immunization with 2 doses of SARS-CoV-2 vaccine, and calculate the positive conversion rate, quantitative results and analysis the consistency of the three methods.Results:The positive conversion rate of SARS-CoV-2 vaccine antibody detected by ELISA, CLIA and NT were respectively 97.9%, 98.6% and 85.3%. The geometric mean of the highest dilution of the serum quantitatively detected by ELISA was 586.6; The mean of CLIA S/CO value was 11.26; The geometric mean titer of the NT was 7.6. The correlation coefficient between ELISA, CLIA and NT were respectively 0.69( P<0.01) and 0.65( P<0.01), and the correlation coefficient between ELISA and CLIA was 0.79( P<0.01). Conclusions:The three methods all detected high levels of antibodies response to SARS-CoV-2 vaccine immunization. ELISA and CLIA are more consistent to detect IgG antibody, and have a good correlation with the quantitative detection results of the NT.
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Objective:To explore whether sleep quality suffers in patients with mild Alzheimer's disease(AD)and mild cognitive impairment(MCI), and to further investigate the correlation between sleep disorders and cognitive function in these patients.Methods:In this study, 30 mild AD patients, 39 MCI patients and 43 demographically matched healthy controls were enrolled.Sleep quality was assessed by the Pittsburgh sleep quality index(PSQI), and cognitive function was assessed by the mini-mental state examination(MMSE), the Montreal cognitive assessment(MoCA)and a set of neuropsychological scales.The correlation of sleep quality with cognitive function was analyzed for the three groups.Results:Differences were significant in sleep time score[0.0(1.0), 1.0(2.0) vs.1.0(1.0), F=8.18, P=0.02]and daytime function score[1.0(1.0), 1.0(1.0) vs.0.0(1.0), F=8.73, P=0.01]between mild AD, MCI and health control groups.Spearman correlation analysis suggested that scores of sleep disorders were negatively correlated with DSB( r=-0.43, P=0.02)and scores of daytime function were positively correlated with ADL( r=0.39, P=0.03)in patients with mild AD.In addition, scores of sleep quality were negatively correlated with the DSB score( r=-0.40, P=0.01), scores of sleep disorders were positively correlated with ADL( r=0.45, P<0.01), scores of daytime function were negatively correlated with DSF( r=-0.42, P=0.01), DSB( r=-0.62, P<0.01)and VFT-S( r=-0.33, P=0.04), and the total PSQI score was negatively correlated with DSF( r=-0.45, P=0.01)and DSB( r=-0.44, P=0.01)in the MCI group. Conclusions:Patients with mild AD and MCI have longer sleep time and impaired daytime function than healthy people, and sleep quality is correlated with memory, attention and daily living ability in patients with mild AD and MCI.
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Objective To explore whether the ability of intertemporal choice is impaired in patients with mild cognitive impairment. Methods Twenty-seven patients with mild cognitive impairment and thirty-five healthy controls were recruited in this study. All subjects completed the " delay-discounting" task and the delayed discount rate (K value) was used to evaluate the patients' decision-making ability. Results (1) The delay discount rate(median=0. 0256,P25=0. 016,P75=0. 100) in mild cognitive impairment patients was higher than that of healthy cotrols(median =0. 006,P25=0. 0025,P75=0. 016). There was significant difference in K-value between the two groups ( Z=-2. 988,P<0. 01). ( 2) Spearman correlation analysis showed that the significant negatively correlation between K-value and digital span forward test (r=-0. 389, P=0. 045) in mild cognitive impairment group. Conclusion The ability of intertemporal choices is impaired in patients with mild cognitive impairment and negatively correlated with attention function.
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Vascular endothelial growth factor (VEGF) plays an important role in angiogenesis and vascular regeneration after cerebral ischemia.On one hand,VEGF plays a neuroprotective effect by promoting angiogenesis and neurogenesis;on the other hand,VEGF induced endothelial permeability can cause blood-brain barrier (BBB) dysfunction.Exercise preconditioning can up-regulate the expression of VEGF and reduce the ischemic brain damage by enhancing the cerebral microvascular integrity.VEGF can be used as a therapeutic target for ischemic stroke.
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Objective To investigate the role of miR-155 in diabetic nephropathy(DN)and its mecha-nism. Methods MiR-155 expression level in kidney was detected by real-time PCR and in situ hybridization. The target gene of miR-155 was predicted by bioinformatics and verified by Western Blot and double luciferase reporter activity. Western Blot was used to detect the related marker proteins of mesangial cells proliferation and mesangial matrix. Results (1)The expression of miR-155 increased in DN renal tissue and high glucose-stimulated renal cells.(2)MiR-155 was related to the regulation of Smad5 gene expression.(3)MiR-155 promoted the mesangial cells proliferation and increased extracellular matrix by down-regulating Smad5 expression. Conclusions MiR-155 can promote the mesangial cells proliferation and renal fibrosis by regulating Smad5 gene,providing a basis for further understanding the pathogenesis of DN.
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Objective To investigate the role of miR-155 in diabetic nephropathy(DN)and its mecha-nism. Methods MiR-155 expression level in kidney was detected by real-time PCR and in situ hybridization. The target gene of miR-155 was predicted by bioinformatics and verified by Western Blot and double luciferase reporter activity. Western Blot was used to detect the related marker proteins of mesangial cells proliferation and mesangial matrix. Results (1)The expression of miR-155 increased in DN renal tissue and high glucose-stimulated renal cells.(2)MiR-155 was related to the regulation of Smad5 gene expression.(3)MiR-155 promoted the mesangial cells proliferation and increased extracellular matrix by down-regulating Smad5 expression. Conclusions MiR-155 can promote the mesangial cells proliferation and renal fibrosis by regulating Smad5 gene,providing a basis for further understanding the pathogenesis of DN.
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Objective: To evaluate the effects of blood lipid stability on progression of carotid atherosclerosis
Methods: A total of 416 patients who had physical examination in our hospital annually from January 2010 to December 2015 were selected and divided into a progression group [n=216] and a non-progression group [n=200] according to the intima-media thickness measured by carotid ultrasound. The levels of lipidrelated parameters within five years were retrospectively analyzed to calculate the smoothness index [SI = x/s]
Results: The cross-sectional TG, HDL-C, ApoAI, ApoB, ApoE and Lpa levels were similar in the two groups [p>0.05]. The non-progression group had significantly higher TC [[4.15 +/- 0.82 vs. 4.50 +/- 1.04] mmol/L] and LDL-C [[2.53 +/- 0.76 vs. 2.99 +/- 1.03] mmol/L] levels than those of the progression group [p<0.05].The progression group had significantly lower TC SI [5.29 +/- 1.28 vs. 5.65 +/- 1.76], TG SI [2.13 +/- 0.71 vs. 2.79 +/- 0.82], LDL-C SI [3.66 +/- 1.17 vs. 4.36 +/- 1.58], ApoB SI [3.37 +/- 0.88 vs. 3.62 +/- 0.95] and Lpa SI [1.53 +/- 0.49 vs. 1.62 +/- 0.43] than those of the non-progression group [p<0.05]
Conclusion: Compared with cross-sectional results, SI was better correlated with the progression of atherosclerosis. The progression group had lower SI values
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The neuroanatomical basis of meridian effects is one of the focuses of acupuncture mechanism studies. Meridian effects depend mainly on the generation, conduction, integration and output of acupuncture information in the acupoint, spinal cord and brain.The neural tracer technique is a basic method in neurobiology. This article presents recent years' advances in the application of the neural tracer technique to acupuncture field and summarizes the types of neural tracer used in acupuncture studies and regularities in the distribution of neurons and their fibers related to labeled meridians and acupoints in spinal segments and brain regions to provide methodological reference for studies on neurobiological mechanism of acupuncture effects.
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Objective To observe the effects ofShenshuaiⅡ Prescription on expressions of pathologic morphology and TNF-α in nephridial tissue of chronic renal failure (CRF) rats induced by 5/6th kidney (ablation/infarction, A/I); To investigate its possible mechanism of action.Methods 5/6 (A/I) nephrectomized CRF rat models were prepared and randomly divided into sham-operation group, model group, Western medicine group and TCM group. Each medication group was given relevant medicine. The levels of serum creatinine (SCr), blood urea nitrogen (BUN), hemoglobin (Hb) and endogenous creatinine clearance (Ccr) were measured after 60 days of administration. The histopathology of residual kidney was observed, and the expression of TNF-α in residual renal tissue was detected by immunohistochemistry.Results Compared with the model group, the levels of SCr and BUN significantly decreased, while Hb and Ccr significantly increased in each medication group (P<0.05,P<0.01). The pathological changes of the residual renal tissue were improved in each medication group. The expression of TNF-α protein in the rats of medication group was significantly lower than that in the Western medicine group (P<0.05, P<0.01).Conclusions henshuaiⅡ Prescription can improve renal function and alleviate changes of renal pathology in CRF rats. The mechanism of anti-renal interstitial fibrosis may be related to the improvement of renal ischemia and hypoxia and the regulation of the expression of TNF-α.
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Objective To understand the association between pregnancy intention and pregnancy-related anxiety in the second and third trimester and its strength.Methods A prospective cohort study was conducted in Ma' anshan,Anhui province.A total of 3 474 eligible pregnant women within 14 weeks of gestation were recruited.The information about their demographic characteristics were collected in early pregnancy.The completed questionnaire of pregnancy-related anxiety were asked to return in the second and third trimester.Logistic regression analysis was conducted to evaluate the association between pregnancy intention and pregnancy-related anxiety in the second and third trimester.Results A total of 3 083 pregnant women were included in final analysis,The rate of unintentional pregnancy was 15.00％ (n=461).The detection rates of pregnancy-related anxiety in the second and third trimester were 29.13％ (n=898) and 30.36％ (n=936).After controlling potential confounding factors,unintentional pregnancy increased the risk of pregnancy-related anxiety in the second trimester compared with intentional pregnancy (OR=1.85,95％ CI:1.44-2.38);The risk of pregnancy-related anxiety also increased in the third trimester (OR=1.84,95％ CI:1.44-2.35).Intentional pregnancy did not increase the risk of pregnancy-related anxiety in the second and third trimester.Conclusion The study results suggests that unintentional pregnancy could increase the risk of pregnancy-related anxiety in the second and third trimester.
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Objective To understand the association between pregnancy intention and pregnancy-related anxiety in the second and third trimester and its strength.Methods A prospective cohort study was conducted in Ma' anshan,Anhui province.A total of 3 474 eligible pregnant women within 14 weeks of gestation were recruited.The information about their demographic characteristics were collected in early pregnancy.The completed questionnaire of pregnancy-related anxiety were asked to return in the second and third trimester.Logistic regression analysis was conducted to evaluate the association between pregnancy intention and pregnancy-related anxiety in the second and third trimester.Results A total of 3 083 pregnant women were included in final analysis,The rate of unintentional pregnancy was 15.00％ (n=461).The detection rates of pregnancy-related anxiety in the second and third trimester were 29.13％ (n=898) and 30.36％ (n=936).After controlling potential confounding factors,unintentional pregnancy increased the risk of pregnancy-related anxiety in the second trimester compared with intentional pregnancy (OR=1.85,95％ CI:1.44-2.38);The risk of pregnancy-related anxiety also increased in the third trimester (OR=1.84,95％ CI:1.44-2.35).Intentional pregnancy did not increase the risk of pregnancy-related anxiety in the second and third trimester.Conclusion The study results suggests that unintentional pregnancy could increase the risk of pregnancy-related anxiety in the second and third trimester.
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Objective To observe microstructural changes of white matter in Alzheimer's disease (AD),and to find out the relationship between white matters lesion and declined cognition.Methods Twenty-four AD patients and 24 controls with normal cognition performed diffusion tensor imaging MR scan.Tract-based spatial statistical analysis (TBSS) was used to investigate microstructural change of white matter,and then its correlation with Mini-Mental State Examination (MMSE) scores was analyzed.Results Fractional anisotropy (FA) values were decreased in corpus callosum,fornix,left corticospinal tract,bilateral cerebral peduncle,superior cerebellar peduncle,internal capsule,corona radiata,posterior thalamic radiation,sagittal stratum including inferior longitidinal fasciculus and inferior fronto-occipital fasciculus,external capsule,cingulate gyrns,hippocampus,superior longitudinal fasciculus and uncinate fasciculus,while mean diffusivity (MD) values were increased in fornix,left hippocampus,bilateral internal capsule,corona radiata,posterior thalamic radiation,sagittal stratum including inferior longitidinal fasciculus and inferior fronto-occipital fasciculus,external capsule,cingulate gyrus,superior longitudinal fasciculus,superior fronto-occipital fasciculus and uncinate fasciculus (all P ＜ 0.05,threshold-free cluster enhancement corrected).MMSE scores had a positive correlation with decreased FA values of left sagittal stratum (r =0.535,P =0.007) and negative correlations with increased MD values of left sagittal stratum (r =-0.427,P =0.037),left superior longitudinal fasciculus (r =-0.424,P =0.039) and left uncinate fasciculus (r =-0.505,P =0.012).Conclusions There exist widespread white matter lesions in AD with impairment of white matter connections of intra-and inter-hemispheres.Impaired white matters in left hemisphere may have close relationships with cognition decline.
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Objective To investigate the effect of neonatal overfeeding on the expression of lipid metabolic associated enzymes and molecular mechanisms in the livers of rats. Methods Male Sprague-Dawley rats were randomly assigned to litter sizes of three group ( small litters,SL group) or ten ( normal litters,NL group) on postnatal day 3. Body weight,milk intake,liver and fat pad (epididymal and retroperitonea) weight,and hepatic histological anal-ysis were recorded in week 2 and week 3,respectively. The levels of lipids were detected by the automatic biochemical analyzer. The mRNA expressions of acetyl-CoA carboxylase ( ACC) ,lipoprotein lipase ( LPL) ,liver-type fatty acid-binding protein (L-FABP), carnitine palmitoyltransferase 1(CPT1),microsomal triglyceride transfer protein (MTP), sterol regulatory element-binding protein-1c (SREBP-1c) and peroxisome proliferator activated receptorα(PPARα) in liver were determined by real time PCR;the protein expressions of SREBP-1c and PPARα were determined by Western lot. Results As early as week 2,the body weight of rats in SL group began to elevate (t=-5. 997,P<0. 001) and food intake (t=-3. 462,P=0. 002) compared with the rats in NL group,and persistent to weaning (body weight:t=-17. 019,P<0. 001;food intake:t=-2. 276,P=0. 031). By the time of 3 weeks old,SL rats increased visceral fat pad [ret-roperitonea (t=-7. 643,P<0. 001),epididymal (t=5. 997,P=0. 001)],liver weight (t=-7. 812,P<0. 001),hepatosomatic index (t=-3.829,P=0. 003) and serum triglyceride (TG) level (t=-2. 703,P=0. 022) compared with those of NL rats,as well as the level of hepatic ACC mRNA (t=-3. 751,P=0. 007),LPL (t=-2. 721,P=0. 017) and L-FABP mRNA (t=-2. 521,P=0. 026) . While CPT1 mRNA (t=-1. 531,P=0. 155) and MTP mRNA (t=-1. 741,P=0. 098) levels remained unchanged in both groups. Hepatic SREBP-1c mRNA expression increased in SL rats after 2 to 3 weeks (t=-2. 836,P=0. 016),paralleled with ACC and LPL mRNA expression;while the mRNA and protein expression of PPARα re-mained unchanged (t=-0. 854,P=0. 411). Conclusions Postnatal overfeeding can promote higher liver pad and dyslip-idemia at the time of weaning. The process may be regulated by up-regulated expression of ACC, LPL and L-FABP. SREBP-1c may be participated in the regulation of ACC,a rate-limiting enzyme involved in lipogenesis.
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<p><b>OBJECTIVE</b>To evaluate the associations between pregestational body mass index (BMI), weight gain during first half of pregnancy and the risk for gestational diabetes mellitus (GDM).</p><p><b>METHODS</b>A prospective cohort study was conducted among 1,914 local pregnant women, receiving the first prenatal examination during the first 14 weeks of gestation, in Ma'anshan of Anhui province from May 2013 to September 2014. The body weight and height were measured for these pregnant women and questionnaire surveys were conducted among them at enrollment, middle gestation and late gestation, respectively. During 24-28 week of gestation, 75 g oral glucose tolerance test was conducted for them. The independent and joint associations between pregestational BMI/weight gain and the risk of GDM were examined by using logistic regression model.</p><p><b>RESULTS</b>The prevalence of GDM was 14.73%. There was significant negative correlation between pregestational BMI and weight gain during the first half of pregnancy (r=-0.085, P<0.01), meanwhile the weight gain of GDM women was significantly higher than that of women without GDM. The women with pregestational overweight or obesity had increased risks of GDM. The results from the logistic regression analysis showed that the risk factors included age≥35 years (OR=3.06, 95% CI: 1.68-5.58), fasting plasma glucose level during early pregnancy (OR=2.17, 95% CI: 1.57-3.00), pregestational overweight (OR=2.08, 95% CI: 1.38-3.13), pregestational obesity (OR=3.73, 95% CI: 1.84-7.56).</p><p><b>CONCLUSION</b>Pregestational overweight or obesity and body weight gain during pregnancy were associated with increased risk of GDM.</p>