RÉSUMÉ
Objective @#To explore the mechanism of mesenchymal epithelial transformation ( EMT) mediated by Wnt/β-catenin signaling pathway in the inhibition of endometrial fibrosis by endometrial mesenchymal stem cells (eMSCs) . @*Methods @#Eighteen female SD rats were randomly divided into Sham group , Model group and eMSCs group , with 6 rats in each group . Rats in Sham group merely had laparotomy without any treatment. A rat model of intrauterine adhesion (IUA) was established in the Model group and eMSCs group . In eMSCs group , the total a- mount of eMSCs cell suspension transplanted immediately after model injury was 0. 05 ml(2 ×107 cells/ml) per u- terus for treatment. Three weeks later , the uterus with unilateral injury was collected for hematoxylin-eosin (HE) staining and Masson staining. Endometrial fibrosis , EMT , Wnt/β-catenin pathway protein expression were analyzed by protein blot. @*Results @#In the Model group , the structure of the uterine cavity was destroyed and the number of glands were significantly reduced with a large number of blue collagen fibers were accumulated . However , after eM- SCs treatment, the number of endometrial glands increased, and the fibrotic area decreased significantly. Compared with Sham group , the expression levels of type I collagen and α-SMA protein in Model group increased significantly (P < 0. 05) , but decreased significantly in eMSCs group (P < 0. 05) . In the Model group , the expressions of N- cadherin , vimentin and ZEB1 increased significantly , while the expression of E-cadherin decreased . However , in eMSCs group , the changes of protein of the above molecules were completely opposite . Compared with Sham group , the expression of β-catenin and C-myc increased in Model group (P < 0. 05) . Compared with the Model group , the expressions of CyclinE , β-catenin and C-myc increased in eMSCs group ( P < 0. 05) .@*Conclusion @#eMSCs can promote endometrial repair in IUA rats by inhibiting EMT and endometrial fibrosis , which is partly achieved by ac- tivating Wnt/β-catenin signaling pathway.
RÉSUMÉ
Objective To evaluate the efficacy of laparoscopic surgery combined with sanjiezhentong capsule for endometriosis. Methods Thirty-three cases of endometriosis who accepted conservative surgery were divided into 2 groups: 19 cases accepted treatment of sanjiezhentong capsule (sanjiezhentong capsule group, infertile patients were 9 cases) and 14 cases accepted goserelin (goserelin group, infertile patients were 8 cases ). The treatment of two groups were given drugs on the first day of menstrual onset after operation for 3 months. The effect, side effect and pregnant was compared after treatment. Results The total effective rate of sanjiezhentong capsule group was 73.7%( 14/19) and in goserelin group was 78.6%( 11/14), there had no statistically significant (P> 0.05). The pregnancy rate of secondary infertile patients in sanjiezhentong capsule group was 55.6%(5/9) and in goserelin group was 62.5%(5/8)(P> 0.05). The side effect was lower in sanjiezhentong capsule group than that in goserelin group. Conclusion Sanjiezhentong capsule is a safe and effective drug in treating post-operative endometriosis.