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Article de Anglais | WPRIM | ID: wpr-210971

RÉSUMÉ

Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.


Sujet(s)
Animaux , Chiens , Structures anatomiques de l'animal/composition chimique , Anticorps antihelminthe/sang , Antigènes d'helminthe/composition chimique , Clonage moléculaire , Dirofilaria immitis/composition chimique , Modèles animaux de maladie humaine , Escherichia coli/génétique , Expression des gènes , Données de séquences moléculaires , Masse moléculaire , Protéines de fusion recombinantes/composition chimique , Analyse de séquence d'ADN , Marqueurs biologiques tumoraux/composition chimique
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