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Article de Chinois | WPRIM | ID: wpr-280304

RÉSUMÉ

The lack of effective in vitro infection model for hepatitis E virus (HEV) has greatly hindered the quantitative analysis of neutralizing titers of anti-HEV antibodies and human sera, thus impeding further studies of HEV-stimulated antibody responses and the immunological mechanisms. In order to improve this situation, the infection of HepG2 cells that are inefficient for HEV replication was continuously monitored until the viral load reached the limit of detection on day 13, the results of which confirmed the feasibility of using this cell line to establish the infection model. Then, neutralization assays of five anti-HEV murine monoclonal antibodies and serum samples collected from four HEV vaccine recipients (collected before and after vaccination) were performed by 96 multi-channel parallel infections, nucleic acid extraction, and qPCR. The results showed that the cell model can be applied for quantitative evaluation of the neutralizing capacity of different antibodies and antiserum samples from HEV vaccine recipients. In this study, we have successfully established a high-throughput in vitro HEV replication model, which will prove to be useful for the evaluation of HEV vaccines and studies of HEV epitopes.


Sujet(s)
Animaux , Humains , Souris , Anticorps antiviraux , Allergie et immunologie , Anticorps de l'hépatite , Allergie et immunologie , Hépatite E , Allergie et immunologie , Virologie , Virus de l'hépatite E , Chimie , Allergie et immunologie , Physiologie , Tests de criblage à haut débit , Méthodes , Souris de lignée BALB C , Tests de neutralisation , Méthodes , Réplication virale
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